• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.180-183
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• 2011

'Yeomyeong', a new welsh onion (Allium fistulosum L.) variety, is developed by the Bioenergy Crop Research Center, NICS, RDA in 2004. This variety, as interspecific $F_1$ hybrid, is developed by a cross between onion (Allium. cepa L.) and welsh onion (A. fistulosum L). The first cross was conducted in 2003 between MOS8, onion male sterile line and G2, welsh onion inbred line. Horticultural and yield characteristics of this hybrid $F_1$ line was investigated in greenhouse for 2 years from 2004 to 2005 with fall cropping cultivation. It has a single pseudostem plant type, anthocyanin-colored pseudostem, and male sterile umbel. It showed intermediate plant type of the maternal parents in overall plant characteristics. 'Yeomyeong' is fall sawing variety and has higher plant height and pseudostem length than that of check variety 'Gumjang'. The yield potential of this variety was about 130.1MT/ha in greenhouse in spring harvseting season. This variety would be adaptable to the fall sowing cultivation in green house.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.6
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• pp.345-350
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• 2006

Recently we have interest on rice products developed by environment-friendly management. The technology of paper mulching was practised without herbicide in machine transplanting cultivation of paddy. A field experiment was conducted on Gangseo series (coarse loamy, mixed, nonacid, mesic family of Aquic Fluventic Eutrochrepts) at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Republic of Korea in 2004. This experiment was carried out to evaluate rice growth, weed control and nitrogen efficiency by the different controlled-release fertilizer levels in paper mulching transplanting. Treatments consisted of conventional fertilization, controlled-release fertilizer (100%, 80%, 60%) compared with nitrogen amount ($110kg\;ha^{-1}$) of conventional fertilization and no nitrogen plot. Mulching paper consisted of recycled paper which was coated with biodegradable plastics. There were no differences between conventional rice transplanting and paper mulching on missing hills. Weed occurrence and control were diverse and low as fertilizer amount increased. Plant height and tiller number per hill increased as fertilizer amount decreased. There were no difference between controlled-release fertilizer 80% and conventional fertilization plot on rice growth traits. Leaf color and $NH_4{^+}-N$ in soil had similar trends. There was no difference in yield between controlled-release fertilizer 80% and conventional fertilization plot. Agronomic nitrogen-use efficiency was high as fertilizer amount decreased. Therefore, these results suggested controlled-release fertilizer 80% should be optimum amount under paper mulching transplanting of rice considering weed control, rice growth and nitrogen efficiency.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.495-500
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• 2007

Recently we are interest in organic farming of rice. The technology of paper mulching was practised without herbicide in machine transplanting cultivation of paddy. A field experiment was conducted in Gangseo series (coarse loamy, mixed, nonacid, mesic family of Aquic Fluventic Eutrochrepts) at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Republic of Korea in 2004. This experiment was carried out to evaluate soil redox potential, weed control and rice growth by the different organic matter in paper mulching transplanting. Compost, rice straw and soiling rye were incorporated as organic matter. The nitrogen amount of controlled-release fertilizer (CRF) plot was 80% compared with nitrogen amount ($110kg\;ha^{-1}$) of conventional fertilization. Mulching paper consisted of recycled paper which was coated with biodegradable plastics. There were no difference between conventional rice transplanting and paper mulching on missing hills after organic matter application. Weed control were the highest at added soiling rye plot. The redox potential of soil was the lowest in rice straw + CRF 80% plot at tillering stage. The $NH_4{^+}-N$ in soil was the highest at soiling rye + CRF 80% tillering stage. There was no difference in yield between soiling rye + CRF 80%, compost + CRF 80% and conventional fertilization plot.

• The Korea Journal of Herbology
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• v.36 no.3
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• pp.15-24
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• 2021

Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 ㎍/㎖). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.

• Korean Journal of Breeding Science
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• v.49 no.4
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• pp.396-402
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• 2017

'Daname' is a foxtail millet(Setaria italica Beauv.) variety developed and registered by Department of Southern Area Crop Science, NICS, RDA in 2014. This variety which collected from IT252182 was developed through pure line selection. 'Daname' is a early-maturing variety having 100 days of growth period from seeding to harvesting in Milyang of Korea. The culm length is about 97 cm which is 20cm shorter culm length than standard variety 'Hwanggeumjo', and it showed tolerant to lodging in field. The panicle shape is a cylindrical and the length is about 19cm. According to the grain, seed color and dehusked grain colors are orange and yellow and endosperm characteristic is non-glutinous. The yield performance of this variety was about $3.81t\;ha^{-1}$ in local adaptability test from 2013 to 2014 and it showed similar with standard variety 'Hwanggeumjo'. As the addition amount of 'Daname' in rice increased from 0% to 30% and 100%, the antioxidant ability of 'Daname' added rice and hardness/stickiness balances were increased. But we could not find out the difference of hardness/stickiness balances between 0%and 10% addition. So, 10% addition amount was concluded to increase antioxidant ability regardless eating quality of cooked rice. (Variety registration No. 6243)

• Korean Journal of Breeding Science
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• v.40 no.1
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• pp.39-47
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• 2008

The 1,874 rice lines were selected from 3,000 Ds insertional mutant pool by Basta herbicide treatment and were surveyed for trait variation and molecular characteristics of genes knocked out by Ds insertion. Compared with "Donjin", an original japonica cultivar used for transformation, Ds insertion mutant pool showed large variation in major agronomic traits including tiller, panicle, and heading etc. Southern blot analysis demonstrated that these lines on the average had two Ds copies in Donjin genome, resulting in 38.4% of one copy, 32.5% of two copies, 16.7% of three copies, and 11.3% of over four copies. GUS analysis showed that 3.9% of lines (73/1,860) had tissue-specific expression in leaves, nodal parts, floral organs such as stigma and pollen, and roots. Data set obtained from agricultural trait variation and molecular characteristics for individual Ds insertional lines would provide researchers with more information for understanding the function of unknown rice genes controlling economically important traits.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.515-524
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• 2010

The amylose content of starch is a major factor in the texture of cooked cereal grains. Therefore, down-regulation of amylose synthesis is one of the alternative method to improve eating quality of rice. We developed transgenic rice plants designed to suppress granule-bound starch synthase I(GBSSI) gene using RNA interference(RNAi) technology. Transgenic plants with RNAi vector containing the 3'-UTR region of GBSSI showed a lower amylose content in rice endosperm than that of wild-type. The range of amylose content was 5.9~9.0% in the transgenic plants, whereas that of wild-type was 17.7~18.0%. Transgenic rices showed the decrease of short chain and the increase of long chain by analyzing chain length distribution of amylopectin in the endosperm. In the SEM micrographs, we found that compound starch granules in whole grains of the wild-type rice were readily split during fracturing, while the starch granules in RNAi-transgenic lines showed small voluminous, non-angular rounded bodies.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.468-473
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• 2009

Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used for chromosome analysis of hybrids (2n=16) between onion (Allium cepa L., 2n=2X=16) and welsh onion (A. fistulosum L., 2n=2X=16). 5S rDNA, 45S rDNA, and tandemly repeated DNA (TSD) sequence were used as probes for FISH analysis. A. fistulosum specific DNA probe of telomeric repeats and A. fistulosum DNA were used for GISH analysis. In the analysis of meiotic chromosome GISH revealed that hybrids have 7 bivalants and 2 univalents chromosome and 2 univalents were derived from A. fistulosum chromosomes. In somatic chromosomes of hybrid each 8 chromosomes were derived from A. cepa and A. fistulosum, respectively. FISH signal of 45S rDNA probe in A. fistulosum was detected at secondary constriction of chromosomes, while FISH signal in A. cepa was observed in both secondary constriction and telomere of chromosomes. TDS signals in A. fistulosum chromosomes were detected at all subtelomeric of 8 chromosomes and also in 2 pericentromeric of the chromosomes, whereas TDS signals in A. cepa were observed only in subtelomeric in all chromosomes. The pattern of TDS signal in hybrid chromosomes was similar to those of A. fistulosum chromosomes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.18-24
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• 2019

Isoflavones, a group of secondary metabolites present in plants and especially abundant in soybeans, are garnering increasing interest because of their medicinal properties. Numerous environmental factors influence isoflavone accumulation in plants. Successful attempts to increase isoflavone contents in soybean seeds have not been widely reported. In this report, we examined an effective method for producing a high amount of isoflavones during soybean seed germination, by treating the seed with 4 abiotic factors. Soybean seeds were exposed to ultrasound (50 Hz) and heat ($40^{\circ}C$) for 30minutes, then germinated for 5 days in darkness. Another sample was subjected to water deficit during germination in darkness, and the other sample was subjected to light conditions during the germination period. The results showed that the ultrasound treatment increased isoflavone content during soybean germination. Three days after germination, the ultrasound-treated seeds presented the highest isoflavone content (6,042.1 ug/g dry weight) compared to the other treatments, which was also 1.3-fold increases over the content of the raw seed's before germination. With respect to each type of isoflavone group, aglycone (partially genistein) content was markedly higher, whereas glucoside was lower than that of the untreated samples. In other experiment, total isoflavone content after heat, water deficit, and light treatments was 5,600.0, 4,740.1, and 5,631.4 ug/g, respectively. These present work suggests that ultrasound treatment is a novel approach for improving the production of isoflavones in soybean seeds during germination.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.2
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• pp.77-83
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• 2021

Rye (Secale cereal L.) is the most tolerant to abiotic stress including low temperature, drought, and unfavorable soil conditions among the winter cereals. Rye is the rapid growth of early spring results from increasing areas for the use of the forage and green manure in the middle part of Korea. "Jungmo2509", a rye cultivar was developed by the National Institute of Crop Science (NICS), RDA in 2014. It was developed from a cross between "Olhomil", a self-compatible cultivar, and "Synthetic II", a self-incompatible line. "Jungmo2509" is an erect plant type and of a middle size, with a green leaf color, a yellowish-white colored culm, and a yellowish brown-colored, small-size grain. The heading date of "Jungmo2509" was April 23, which was 5 days later than that of "Gogu", respectively. But "Jungmo2509" showed greater resistance to lodging compared to that of the check cultivar, with similar to winter hardiness, wet injury, and disease resistance. "Jungmo2509" was a higher to than "Gogu" in terms of protein content (9.4% and 8.0%, respectively), total digestible nutrients (TDN) (55.7% and 55%, respectively). The seed productivity of "Jungmo2509" was approximately 2.08 ton 10a-1, which was 11% lower than that of the check. Almost all rye cultivars are out-crossing due to genes controlling incompatibility, but "Jungmo2509" is higher seed fertility (56%) than that of Gogu (0%). it has self-compatible genes. "Jungmo2509" is erect in plant type and resistance for lodging. Therefore, "Jungmo2509" can produce uniform seeds for processed grains of human consumption and utilize them as parents for breeding the rye hybrids with high forage yields.