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Trait Variation and Molecular Characterization of Ds insertional rice lines  

Ahn, Byung-Ohg (National Institute of Agricultural Biotechnology)
Kang, Kyung-Ho (National Institute Crop Science)
Eun, Moo-Yong (National Institute of Agricultural Biotechnology)
Jeon, Yong-Hee (National Institute Crop Science)
Yun, Doh-Won (National Institute of Agricultural Biotechnology)
Ji, Hyeon-So (National Institute of Agricultural Biotechnology)
Park, Sung-Han (National Institute of Agricultural Biotechnology)
Nam, Min-Hee (Yeongnam Agricultural Research Institute, NICS)
Suh, Seok-Cheol (National Institute of Agricultural Biotechnology)
Lee, Myung-Chul (National Institute of Agricultural Biotechnology)
Publication Information
Korean Journal of Breeding Science / v.40, no.1, 2008 , pp. 39-47 More about this Journal
Abstract
The 1,874 rice lines were selected from 3,000 Ds insertional mutant pool by Basta herbicide treatment and were surveyed for trait variation and molecular characteristics of genes knocked out by Ds insertion. Compared with "Donjin", an original japonica cultivar used for transformation, Ds insertion mutant pool showed large variation in major agronomic traits including tiller, panicle, and heading etc. Southern blot analysis demonstrated that these lines on the average had two Ds copies in Donjin genome, resulting in 38.4% of one copy, 32.5% of two copies, 16.7% of three copies, and 11.3% of over four copies. GUS analysis showed that 3.9% of lines (73/1,860) had tissue-specific expression in leaves, nodal parts, floral organs such as stigma and pollen, and roots. Data set obtained from agricultural trait variation and molecular characteristics for individual Ds insertional lines would provide researchers with more information for understanding the function of unknown rice genes controlling economically important traits.
Keywords
Rice; Ac/Ds; Trait variation; Herbicide resistance; GUS;
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