• Journal of Food Hygiene and Safety
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• v.18 no.1
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• pp.6-13
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• 2003

Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy$^{TM}$, Wizard$^{TM}$, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.

• The KIPS Transactions:PartB
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• v.10B no.2
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• pp.229-236
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• 2003

Named entity recognition is the process indispensable to question answering and information extraction systems. This paper presents an HMM based named entity (m) recognition method using the construction principles of compound words. In Korean, many named entities can be decomposed into more than one word. Moreover, there are contextual relationships among nouns in an NE, and among an NE and its surrounding words. In this paper, we classify words into a word as an NE in itself, a word in an NE, and/or a word adjacent to an n, and train an HMM based on NE-related word types and parts of speech. Proposed named entity recognition (NER) system uses trigram model of HMM for considering variable length of NEs. However, the trigram model of HMM has a serious data sparseness problem. In order to solve the problem, we use multi-level back-offs. Experimental results show that our NER system can achieve an F-measure of 87.6% in the economic articles.

• Fisheries and Aquatic Sciences
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• v.20 no.7
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• pp.9.1-9.13
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• 2017

Background: Metal-responsive transcription factor-1 (MTF-1) is a key transcriptional regulator playing crucial roles in metal homeostasis and cellular adaptation to diverse oxidative stresses. In order to understand cellular pathways associated with metal regulation and stress responses in Pacific abalone (Haliotis discus hannai), this study was aimed to isolate the genetic determinant of abalone MTF-1 and to examine its expression characteristics under basal and experimentally stimulated conditions. Results: The abalone MTF-1 shared conserved features in zinc-finger DNA binding domain with its orthologs; however, it represented a non-conservative shape in presumed transactivation domain region with the lack of typical motifs for nuclear export signal (NES) and Cys-cluster. Abalone MTF-1 promoter exhibited various transcription factor binding motifs that would be potentially related with metal regulation, stress responses, and development. The highest messenger RNA (mRNA) expression level of MTF-1 was observed in the testes, and MTF-1 transcripts were detected during the entire period of embryonic and early ontogenic developments. Abalone MTF-1 was found to be Cd inducible and highly modulated by heat shock treatment. Conclusion: Abalone MTF-1 possesses a non-consensus structure of activation domains and represents distinct features for its activation mechanism in response to metal overload and heat stress. The activation mechanism of abalone MTF-1 might include both indirect zinc sensing and direct de novo synthesis of transcripts. Taken together, results from this study could be a useful basis for future researches on stress physiology of this abalone species, particularly with regard to heavy metal detoxification and thermal adaptation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.6
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• pp.1666-1672
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• 2005

Oriental medicinal drugs have a broad spectrum of clinical use for the cure of nervous system diseases including brain ischemic damages or neuropathies. Yet, specific drugs or drug components used in the oriental medicine in relation to none fiber regeneration are not known. In the present study, possible growth promoting effects of oriental medicinal drugs were investigated in the injured sciatic nerve system in the rat. By immunofluorescence staining, we found that Jahageo (JHG, Hominis placenta) increased Induction levels of axonal growth associated protein GAP-43 in the rat sciatic none. Small growth promoting activity was found in Golsebo (GSB, Drynariae rhizoma) and Baikhasuo (BHSO, Polygoni multiflori radix) drugs. JHG also increased cell cycle protein Cdc2 levels in the injured area of the sciatic nerves. Immunofluorescence staining indicated that induced Cdc2 protein was mostly localized in the Schwann cells in the injury area, implying that JHG activity might be related to increased Schwann cell proliferation during axonal regeneration. Moreover, levels of phospho-extracellular signal-regulated (ERK) pathway in the injured neNes were elevated by JHG treatment while levels of total ERK were unaltered. In vivo measurement of axonal regeneration using retrograde tracer showed that JHG, GSB and BHSO significantly enhanced Dil-labeled regenerating motor neurons compared with saline control. The present data suggest that oriental medicinal drugs such as JHG, GSB, and BHSO may be a useful target for developing specific drugs of axonal regeneration.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.5
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• pp.1189-1197
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• 2011

To manage sensing information such as temperature, humidity and so on efficiently, it is need to use special purpose protocol. In this reason, IETF WG proposed CoAP protocol, and it is on Internet draft. If it is possible to work on a specific protocol, sensor end-nodes and network devices will be managed efficiently. However, end-nodes have restricted resources, it is hard to applying to CoAP protocol directly. In this paper we analyse a CoAP protocol stack for USN. To verify this protocol quickly, at first we implemented CoAP protocol stack over PC environments. After the logical verification, we applied this protocol to the USN environment. To do this, we ported CoAP protocol to Cygwin environment, and proposed solutions for hardware dependencies, and it is verified through experiments.

• Korea Trade Review
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• v.41 no.4
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• pp.41-59
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• 2016

Given the increasing importance of entrepreneurship in a nation's economic growth, this study empirically examined the effect of entrepreneurial behavior and environment on economic growth based on country level data. While previous studies have centered on entrepreneurship as a dominant variable that impacts economic growth, this study has extends the discussion by empirically testing the effects of two entrepreneurial variables, which are entrepreneurial behavior and entrepreneurial environment, on economic growth. Furthermore, the study attempted to examine the moderating effects of trade and innovation on the relation between the independent variables and economic growth. According to the panel analysis using data from GEM and World Bank, both entrepreneurial behavior and environment affected national economic growth, completely or partly. Results showed that opportunity-driven entrepreneurial behavior has a significant effect on economic growth, suggesting that qualitative aspect of entrepreneurial activities are critical to economic growth. The results also indicated that both trade and innovation have significant moderating effects on the relationship between governmental support program and economic growth.

• Mycobiology
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• v.40 no.2
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• pp.117-123
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• 2012

Nuruk contributes to the unique characteristics of Korean alcoholic beverages. In this study, the effects of nuruk extracts (NE) on anti-oxidant characters, melanogenesis, and anti-photoaging activity were investigated. NEs were obtained from the 70% ethanol extracts of six types of nuruk, which have been used in brewing of fermented alcohol beverages in Korea. First, various antioxidant characteristics were identified in terms of 2,2'-azino-bis(3-ethylbenzthiozoline-6-sulphonic acid) (ABTS) radical scavenging activity, superoxide dismutase (SOD) expression, and inhibition of xanthine oxidase activity. NE#4 exhibited potent ABTS radical scavenging activity ($IC_{50}$ = 19.51 ${\mu}g$/mL). Compared with NE#4, relatively lower levels of activity were observed for NE#3 and NE#6, with $IC_{50}$ values of 90.99 and 76.88 ${\mu}g$/mL, respectively. According to results of western blot analysis for determination of SOD expression in $H_2O_2$-treated HepG2 cells, NE#5 and NE#6 induced a dramatic increase in the expression ratio of SOD, compared to the group treated with $H_2O_2$ only. Activity of xanthine oxidase, which converts xanthine into uric acid, generating superoxide ions, was inhibited by NE#4 and NE#6 in a dose-dependent manner. NE#4 induced significant inhibition of mushroom tyrosinase activity. A reduction in cellular melanin contents of 80% was observed in B16F1 melanocytes treated with NE#5 and NE#6; these effects were similar to those of arbutin at 100 ${\mu}M$. In addition, gelatin zymography and reverse transcription-PCR analysis were performed for assessment of anti-photoaging activity of Nuruk. Treatment with NE#6 resulted in dramatically inhibited activities of matrix metalloproteinase (MMP)-2/-9, suppressed expression of MMP-1, and increased expression of type-1 procollagen. Results of gelatin zymography for NE#4 and NE#5 were similar, to a slightly lesser degree. These results suggest the potential of NE#4 and NE#6 as natural ingredients for use in functional foods and cosmetics.

• Journal of Korean Public Health Nursing
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• v.9 no.2
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• pp.33-42
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• 1995

The purpose of this study is to identify the smoking patterns and their relevant factors of the public officials who are placed to guide people's way of thinking as conservative middle-income group and thereupon, provide for basic data which can be used to reduce the diseases and death rate caused by smoking in terms of preventive medicine. For this purpose, 170 public officials working for the government agencies located in seoul were surveyed for Jan. 10-Jan. 20, 1995. The results of survey can be analyzed/summarized as follows; 1. In terms of daily smoking frequencies, 50 officials $(29.4\%)$ are driven by stress to smoke. Among these offcials, 250nes $(50.0\%)$ smoke 20-30 times a day. Meanwhile, 22 officials $(12.9\%)$ answered that they smoke for pleasure and comfort, and among them 6 officials $(21.4\%)$ smoke 20-30 times a day. 26 officials $(15.3\%)$ are found to be nicotine­addicted physically or psychologically, and to 10 of them $(35.7\%)$ smoke 20-30 times a day. On the other hand, 10 officials $(5.9\%)$ have habit of smoking and 4 of them $(40.0\%)$ smoke 20-30 times a day. 2. Out of 50 officials $(24.9\%)$ who began smoking on impulse, 44 actually stopped smoking, and the rest 6 officials did not even implement their plan. In view of successful cigarette-stopping, only 8 officials $(15.4\%)$ out of 44 impulse-driven smokers succeeded in stopping cigarettes, while the rest 36 failed all. Meanwhile, out of those 84 officials $(64.6\%)$. Who smoked for reduced stress, 28 ones succeeded, while the rest 56 ones failed. Out of 20 officials who were nicotine-addicted but attempted to stop smoking, only 2 smokers succeeded and the rest 18 were all failures. 3. Factors involving smoking and Their patterns 1) By age group (30's, 40's and 50's), the smoking patterns can be grouped into stress-removing, nocotine-addictive and habitual ones, respectively. 2) By acadeic background, our officials smoke on impulse or due to nocotine addition. 3) The length of career is co-related with nicotine addition. 4) The ownership of house is related with smoking for comfort or less stress. 5) The number of children is related with impulse-driven smoking or smoking for comfort.

• Korean Journal of Animal Reproduction
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• v.10 no.1
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• pp.100-108
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• 1986

Eik-Nes (1966) reported that the mechanism of spermatogenesis is controlled by FSH and LH and maintaned normally in scrotum terperautre which is 3-5$^{\circ}C$ lower than body termperature. But Ojeda and Ramirez (1972) have described that the abdominal testis was shrinked severely and lost its normal function in congenital cryptorchidism or surgically induced cryptorchidism. Ramirez and Sawyer (1974) reported that the compensatory hypertorphy occured in the remaining testis of unilateral castration and the scrotal testis of unilateral cryptorchidism. Cunninham et al. (1978) reported that the serum FSH levle increased after unilateral castration. Frankel and Wright (1982) reported that the serum LH level was unchanged greatly after unilateral castration. Gomes and Jain (1976) reported that the serum testosterone level increased temporarily but not varied after unilateral castration. On the other hand, Kormano et al. (1964) reported that the serum FSH level in unilateral cryptorchidism rat was unchanged in contrast with the control and Risbirdger et al. (1981) reported that the serum LH level was unchanged till 2 weeks after operation and after then increased to 77%. Kim (1984) reported that the serum testosterone level was somewhat lower than that fo control group but there was't significant different. There were many different reports on hormone levels among different investigators when the immarue rats were castrated unilaterally or induced cryptorchidism unilaterally. Liang and Liang (1970) and Cunningham et al. (1978) described that there were no true compenastory hypertrophy in the remaining testis of unilateral castration and scrotal testis of unilateral testis of unilateral cryptorchidism in rat but they grew faster than that of control. Kormano et al.(1964), Damber et al.(1976), Cunningham et al.(1978) and Karpe et al.(1981) reported that the testis weight, germinal epithelia height and seminiferous tubules diameter developed continuously and similarily in the control, the remaining testis of unilateral castration and scrotal testis of unilateral cryptorchidism increased, however, in the abdominal testis of the unilateral cryptorchidism, they were much smaller than those of other groups. In observation of the histological changes in the seminiferous epithelium of control, remaining tesis of unilateral castration and scrotal testis of unilateral cryptorchidism differentiated and developed fully(Cunningham et al., 1978). However, the abdominal testis of unilateral crytorchidism degenerated severely and only the germ cells in early stage and Sertoli cells were found in the seminiferous tubules. (Damber et al., 1976, Gomes and Jain, 1976 and Karpe et al., 1981). By electron microscopic observation, Nagano (1963) and Leason and Leeson (1970) found that the abdominal testis of unilateral cryptorchidism was thicked in boundary tissue, increased lipid droplet in the Sertoli cell, disarranged axial filament complex and increased lipid inclusions in the Sertoli cell.

• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.846-855
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• 2002

Several soft white spring and winter wheat cultivars were evaluated by analyzing physicochemical properties such as Single Kernel Characterization System (SKCS), milling properties, Rapid Visco-Analyzer (RVA), mixograph pattern, and sugar-snap cookie-making potentialities. Results of SKCS revealed kernel hardness had a positive correlation coefficient with test weight $(r=0.497^{*})$ and near-infrared reflectance (NIR) hardness $(r=0.495^{*})$. SKCS kernel weight had a significantly high correlation of $r=0.942^{**}$ with SKCS kernel size. The test weight had significantly high correlations with straight-grade flour yield $(r=0.720^{**})$, break flour yield $(r=0.490^{*})$, flour ash content $(r=-0.781^{**})$, and milling score $(r=0.838^{**})$. The average RVA peak viscosity of the soft white winter wheat was higher (195.1 unit) than the soft white winter wheat varieties (135.7 unit). A correlation was found between RVA peak viscosity and swelling volume. Significant correlation coefficients were obtained among cookie properties, milling properties, protein content, and mixograph absorption. The cookie top grain score had a correlation coefficient of $r=0.447^{*}$ with swelling volume.