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Comparative Evaluation on Qualitative PCR using Different Extraction Methods for Nucleic Acids on Soybean and Corn Processed Foods  

김영찬 (한국보건산업진흥원)
이철수 (한국보건산업진흥원)
황순욱 (한국보건산업진흥원)
김성조 (한국보건산업진흥원)
이영옥 (한국보건산업진흥원)
윤성원 (한국보건산업진흥원)
서정화 (한국보건산업진흥원)
남용석 ((주)코젠바이오텍)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.1, 2003 , pp. 6-13 More about this Journal
Abstract
Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy$^{TM}$, Wizard$^{TM}$, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.
Keywords
PCR; GMO; DNA; Promoter; Terminator;
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