• 약학회지
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• 제53권4호
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• pp.189-193
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• 2009

To investigate the antioxidant effect of lutein on N-nitrosodimethylamine (NDEA)-induced oxidative stress in mice, we measured lipid peroxidation, superoxide dismutase (SOD) and catalase of various tissues. Body weight was almost similar in lutein and control groups during 3 weeks. NDEA increased significantly the activities of typical marker enzymes of liver function (AST, ALT and ALP) in both groups. However, the increase of plasma aminotransferase activity significantly decreased in lutein group. Lipid peroxidation and SOD in various tissues, such as heart, lung, liver, kidney, spleen and plasma were significantly increased by NDEA, which were significantly reduced by lutein at a dose of 50 mg/kg. Catalase activity decreased significantly in control and lutein groups treated with NDEA, the effect being less in lutein group. Lesser effect on SOD and catalase in NDEA-treated lutein group indicates the improvement of protective mechanisms by lutein. Thus, it can be concluded from the present study that lutein can offer a useful protection against NDEA-induced oxidative stress.

• Applied Microscopy
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• 제39권4호
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• pp.283-289
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• 2009

본 실험은 N-nitrosodiethylamine (NDEA)와 사염화탄소($CCl_4$) 로 유발된 흰쥐의 간암에 대한 인진호탕의 효과를 알아보고자 하였다. 먼저 실험군 설정은 세 군으로 분류하였다. 정상군(Nor)에는 일반적인 식이를 공급하였고, 대조군(Con)에는 NDEA(200 mg/kgb.w., i.p.)와 $CCl_4$를 투여하였고, 인진호탕 추출액 투여군(IJH)에는 인진호탕 추출액(260 mg/kg/day)을 8주 동안 투여하였다. Superoxide dismutase (SOD)와 catalase와 같은 항산화계 효소를 측정한 결과, SOD의 활성은 Con군에서 증가하였으나, catalase의 활성은 Con군에서 감소하였다. 면역조직화학적 관찰에서, 인진호탕 추출액의 투여로 인해 Con군에서보다 p53 면역 반응율을 감소시켰다. 전자현미경 관찰의 경우, 간암 세포들은 Con군에서 과립세포질세망의 팽대와 무과립세포질세망의 증가가 뚜렷하게 관찰되었다. 이상의 결과로 보아 인진호탕 추출액 투여가 NDEA와 사염화탄소로 유도된 흰쥐의 간암을 억제 또는 지연하는 것으로 사료된다.

• The Korean Journal of Physiology and Pharmacology
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• 제13권4호
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• pp.321-326
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• 2009

The antioxidant effect of $CoQ_{10}$ on N-nitrosodiethylamine (NDEA)-induced oxidative stress was investigated in mice. Food intake and body weight were similar in both $CoQ_{10}$ and control groups during the 3-week experimental period. NDEA significantly increased the activities of typical marker enzymes of liver function (AST, ALT and ALP) both in control and $CoQ_{10}$ groups. However, the increase of plasma aminotransferase activity was significantly reduced in the $CoQ_{10}$ group. Lipid peroxidation in various tissues, such as heart, lung, liver, kidney, spleen and plasma, was significantly increased by NDEA, but this increase was significantly reduced by 100 mg/kg of $CoQ_{10}$. Superoxide dismutase activity increased significantly upon NDEA-induced oxidative stress in both the control and $CoQ_{10}$ groups with the effect being less in the $CoQ_{10}$ group. Catalase activity decreased significantly in both the control and $CoQ_{10}$ groups treated with NDEA, again with the effect being less in the $CoQ_{10}$ group. The lesser effect on superoxide dismutase and catalase in the NDEA-treated $CoQ_{10}$ group is indicative of the protective effect $CoQ_{10}$. Thus, $CoQ_{10}$ can offer useful protection against NDEA-induced oxidative stress.

• Toxicological Research
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• 제14권2호
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• pp.177-181
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• 1998

Dithiocarbamate and mixed disulfide containing allyl functions were designed and synthesized as putative chemopreventive agents, i.e. N,N-diallyldithiocarbamate (DATC) and S-(N,N-diallyldithiocarbamoyl)-N-acetylcysteine (AC-DATC). DATC and AC-DATC were administered and the activities of cytosolic glutathione S-transferase (GST), glutathione reductase (GR) and microsomal N-nitrosodiethylamine (NDEA) deethylase were assayed in order to test the effects of these organosulfur com-pounds on the detoxification and metabolic activation system of NDEA. The amounts of hepatic glutathione (GSH and GSSG) was also determined. The administration of DATC to rats led to an increase in the activity of GR and to an inhibition of CYP2E1-mediated NDEA deethylation. AC-DATC induced the activity of GR and GST, increased the hepatic GSH content and inhibited the rate of NDEA deethylation. The level of GSSG was decreased as a consequence of the increased activity of GR. These effects may contribute to possible antimutagenic and anticarcinogenic action of the dithiocarbamates investigated.

• 대한환경공학회지
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• 제36권1호
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• pp.49-57
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• 2014

본 연구에서는 낙동강에서의 nitrosamine류의 검출 현황에 대해 조사하였다. 본 연구결과에 따르면 6종의 nitrosamine류 (NDEA (N-nitrosodiethylamine), NDPA (N-Nitrosodi-n-propylamine), NDMA (N-nitrosodimethylamine), NMEA (N-nitrosomethylethylamine), NDBA (N-nitrosodi-n-butylamine) 및 NDPHA (N-Nitrosodiphenylamine))가 낙동강에서 검출되었다. 검출된 nitrosamine류 6종 중에서 NDEA와 NDPA가 주요 오염물질로 나타났으며, 몇몇 지점들에서의 NDEA의 검출농도는 미국 CDHCS (California Department of Health Care Services)의 대응수준인 100 ng/L를 초과하였으며, NDPA의 경우는 소수의 지점들에서 CDHCS의 대응수준(500 ng/L)에 근접한 농도로 검출되었다. 낙동강에서 검출된 nitrosamine류 9종의 최대 검출농도는 735.7 ng/L로 나타났다.

• 한국식품영양과학회지
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• 제38권4호
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• pp.436-441
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• 2009

본 실험은 N-nitrosodiethylamine(NDEA)와 사염화탄소 ($CCl_4$)로 유발된 흰쥐의 간암 생성에 대한 인진호탕의 효과를 조사하였다. 실험은 8주 동안 실시하였고, 세 군으로 분류하였다. 정상군(Nor), 간암을 유발한 대조군(Con), 간암 유발 후 인진호탕 추출액(260 mg/kg/day)을 투여한 실험군 (IJH)으로 구분하였다. 체중은 정상군에 비하여 대조군에서 유의적으로 감소하였지만 실험군에서는 증가하였다. NDEA로 유발된 흰쥐의 혈청 AST, ALT, LDH, ALP와 AFP의 수준은 증가하였다. 반대로, 인진호탕 추출액을 처리했을 때, 혈청 AST, ALT, LDH, ALP와 AFP의 수준이 감소하였다. 흰쥐의 간에서 bcl-2 mRNA의 수준은 대조군에 비해 실험군에서 증가되었다. 그러나 c-myc mRNA의 수준은 대조군에 비해 감소하였다. 또한 NDEA로 손상된 간세포 조직의 공포화가 인진호탕 추출액의 투여로 감소하는 것을 관찰 할 수 있었다. 이상의 결과로 보아 인진호탕 추출액 투여가 NDEA와 사염화탄소로 유도된 흰쥐의 간암 생성을 억제 또는 지연하는 것으로 사료된다.

• 한국식품위생안전성학회지
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• 제12권2호
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• pp.125-131
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• 1997

Gulbi, salted and dried yellow corvenia, is a favorite diet food in Korea from the ancient times. A Few studies have dealt with sanitary concerns related to its products, while a number of investigators studied its taste compounds. This study attemps to establish the basic data for ensuring the safety of Gulbi. The contents of nitrate and nitrite were detected 1.2 and <0.1 mg/kg in raw sample, but their contents were detected 3.6~3.9 and 1.2~2.0 mg/kg during its processing and storage of Gulbi, respectively. TMAO contents decreased while betaine, TMA, DMA and formaldehyde increased in yellow corvenia during its processing and storage. Recovery from raw, salted, salt-dried and stored sample spiked with 10$\mu\textrm{g}$/kg for NDBA was 83.2~102.7% average 92.7%). N-nitrosamine such as NDMA, NDEA and NDPA was not detected in raw sample and drastically increased during Gulbi processing and storage. The levels of NDMA, NDEA and NDPA in Gulbi stored for 30 days were about 2.7,4.5 and 5.3 times higher than those in salted sample, respectively. Regardless of cooking methods, NDMA, NDEA and NDPA during cooking when sample were cooked using direct heating methods such as a gas and a briquet fire than when sample were cooked using indirect methods such as an microwave oven and a fried pan. Indirect cooking methods was effective to minimize the N-nitrosamine formation such as NDMA, NDEA and NDPA during cooking of Gulbi.

• Industrial Engineering and Management Systems
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• 제14권4호
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• pp.347-371
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• 2015

A hybrid procedure based on multi-Criteria Satisfaction Analysis (MUSA) and a Network Data Envelopment Analysis (NDEA) is proposed to evaluate the relative efficiency of customer services in bank branches. First, a three-stage process including sub-processes such as customer expectations, customer satisfaction, and customer loyalty, is defined to model the banking customer services. Then, fulfillment of customer expectations, customer loyalty level, and the customer satisfaction degree are measured and quantified through a multi-dimensional questionnaire based on customers' perceptions analysis and MUSA method, respectively. The customer services scores and the other criteria such as mean of employee evaluation score, operation costs, assets, deposits, loans, number of accounts are considered in network three-stage DEA model. The proposed NDEA model is formed based on multipliers perspective, output-oriented, and constant return to scale assumptions. The proposed NDEA model quantifies and assesses the total efficiency of main process and assigns the efficiency to customer expectations, customer satisfactions, and customer loyalties sub-processes in bank branches. The whole procedure is applied on 30 bank branches in IRAN. The proposed approach can be used in other organizations such as airports, airline agencies, urban transportation systems, railway organizations, chain stores, chain restaurants, public libraries, and entertainment centers.

• 생약학회지
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• 제45권1호
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• pp.62-68
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• 2014

The present study was carried out to investigate the hepatoprotective effect of hot water extract of Korean white dandelion (Taraxacum coreanum Nakai) on liver cancer induced in rats by a single dose of N-nitrosodiethylamine (NDEA). The animals were divided into three groups. The animals in the normal (Nor) group were fed basal diet. Control (Con) group were administered with NDEA (200 mg/kg, i.p.). Hot water extract of Korean white dandelion (KDDA, KDDB) group treated with hot water extracts of Korean white dandelion (30, 60 mg/kg/day) for 8 weeks after NDEA. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and histological alternation of liver were examined by light microscopy. KDDA and KDDB group were decreased the increase of serum AST, ALT level induced by NDEA. The activities of SOD and CAT were significantly increased in KDDA, KDDB then in the Con. In the histological observation, KDDA and KDDB group were structure of liver lobula and nuclear shape was relatively cleared. In addition, the cytoplasm staining was similar to those of the Nor group. vacuolization of cytoplasm was not observed. These results suggest that administration of hot water extract of Korean white dandelion suppress or retard liver cancer induced by NDEA in rats.

• 한국물환경학회지
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• 제26권3호
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• pp.446-453
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• 2010

This study was investigated nine nitrosamines in small tributary rivers, sewage treatment plants (STPs) and drinking water treatment plants. They are N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosopyrrolidine (NPYR), N-nitrosodi-n-propylamine (NDPA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine (NPIP), N-nitrosodi-n-butylamine (NDBA) and N-nitrosodiphenylamine (NDPHA). The nine nitrosamines were analyzed by gas chromatography mass spectrometry (GC/MS) using solid phase extraction (SPE) with a coconut charcoal cartridge. Among the nine nitrosamines, NDMA, NMEA, NDEA, NDPA NDBA and NDPHA were detected in small tributary rivers and sewage tretment plants. In small tributary rivers, NDMA, NMEA, NDEA, NDPA, NDBA and NDPHA were obtained as ND~16.4 ng/L, ND~17.7 ng/L, ND~102.4 ng/L, ND~455.4 ng/L, ND~330.1 ng/L and ND~161.0 ng/L, respectively. Also NDMA, NMEA, NDEA, NDPA and NDBA were investigated ND~821.4 ng/L, 22.5~55.4 ng/L, 53.2~588.5 ng/L, ND~56.6 ng/L and ND~527.9 ng/L in STPs, respectively. In drinking water treatment plants, NMEA and NDEA concentration were increased to as high as 38.8 ng/L after ozonation process. However nitrosamines were decreased subsequent biological activated carbon (BAC) treatment process. It was supposed that nitrosamines were formed by $O_3$ oxidation and were removed by biodegradation of BAC.