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http://dx.doi.org/10.3746/jkfn.2009.38.4.436

The Effect of the Injinhotang Extract on the Hepatocarcinogenesis Induced by N-Nitrosodiethylamine and Carbon Tetrachloride in Rats  

Yoon, Jung-Sik (Dept. of Anatomy, College of Oriental Medicine, Dongshin University)
Kim, Jeong-Sang (Dept. of Anatomy, College of Oriental Medicine, Dongshin University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.4, 2009 , pp. 436-441 More about this Journal
Abstract
In order to examine the effect of Injinhotang extract on the hepatocarcinogenesis induced by N-nitrosodiethylamine (NDEA) and carbon tetrachloride ($CCl_4$) in 8 week-old rats. Experimental rats were subdivided into three groups; normal group (Nor), hepatic cancer inducing control group (Con), and control group administered Injinhotang extract 260 mg/kg/day (IJH). The body weight decreased significantly (p<0.05) in the Con compared with the Nor. The body weight of IJH group more increased than Con. Rats intoxicated with NDEA had significantly (p<0.05) increased levels of serum AST, ALT, LDH, ALP, and AFP. On the contrary, group treated with Injinhotang extract had inhibited levels of serum AST, ALT, LDH, ALP, and AFP. The bcl-2 mRNA expression levels in rat liver were more increased in the IJH than Con, but these levels of c-myc mRNA were more decreased in the IJH than Con. Also, cytoplasmic vacuolizations in the liver of NDEA-administrated rats were inhibited by the treatment of Injinhotang extract. These results suggest that administration of Injinhotang extract suppresses or retards NDEA and $CCl_4$-induced hepatocarcinogenesis in rats.
Keywords
Injinhotang; N-nitrosodiethylamine; hepatocarcinogenesis; AFP; bcl-2;
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