Browse > Article

The Effect of the Injinhotang Extract on Hepatocarcinogenesis in Rats  

Yoon, Jung-Sik (Department of Anatomy, College of Oriental Medicine, Dongshin University)
Kim, Jeong-Sang (Department of Anatomy, College of Oriental Medicine, Dongshin University)
Publication Information
Applied Microscopy / v.39, no.4, 2009 , pp. 283-289 More about this Journal
Abstract
In order to examine the effect of Injinhotang extract on the liver cancer induced by N-nitrosodiethylamine (NDEA) and carbon tetrachloride ($CCl_4$) in Rats. The animals were divided into three groups. The normal (Nor) group were fed basal diet. Control (Con) group were administered with NDEA (200 mg/kgb.w., i.p.) and $CCl_4$. Injinhotang extract (IJH) group treated with Injinhotang extract (260 mg/kg/day) for 8 weeks after NDEA+$CCl_4$. Enzymic antioxidants, such as superoxide dismutase (SOD) and catalase levels were determined in all the groups of animals. The activities of SOD were significantly increased in the Con, but the activities of catalase were decreased in the Con, but the anti-oxidative enzyme activities of superoxide dismutase and catalase were increased in the IJH. In the immunohistochemistry observation, treatment of Injinhotang extract reduced the rates of p53 immunoreactivity. According to the electron microscopical observation, in the liver cancer cells were increased the smooth endoplasmic reticulum and dilated the rough endoplasmic reticulum in the Con compared with IJH. These results suggest that administration of Injinhotang extract suppress or retard NDEA and $CCl_4$-induced liver cancer.
Keywords
Injinhotang extract; Liver cancer; N-nitrosodiethylamine; Antioxidants; p53;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bourdon JC, D'Errico A, Paterlini P, Grigioni W, May E, Debuire B: p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation. Gastroenterology 108 : 1176-1182, 1995   DOI   ScienceOn
2 Collier JD, Carpenter M, Burt AD, Bassendine MF: Expression of mutant p53 protein in hepatocellular carcinoma. Gut 35 : 98-100, 1994   DOI   ScienceOn
3 Han KH, Jeon YJ, Athukorala Y, Choi KD, Kim CJ, Cho JK, Sekikawa M, Fukushima M, Lee CH: A water extract of Artemisia capillaris prevents 2,2'-azobis(2-amidinopropane) dihydrochloride-induced liver damage in rats. J Med Food 9 : 342-347, 2006   DOI   ScienceOn
4 Kovalszky I, Szeberenyi S, Zalatnai A, Vincze I, Lapis K, Jeney A: Modification of DENA-induced hepatocarcinogenesis by $CCl_4$ cirrhosis. Comparison of the marker enzyme patterns. Carcinogenesis 13(5) : 773-778, 1992   DOI   ScienceOn
5 Lee J, Chae K, Ha J, Park BY, Lee HS, Jeong S, Kim MY, Yoon M: Regulation of obesity and lipid disorders by herbal extracts from Morus alba, Melissa officinalis, and Artemisia capillaris in high-fat diet-induced obese mice. J Ethnopharmacol 115 :263-270, 2008   DOI   ScienceOn
6 Legros Y, Meyer A, Ory K, Soussi T: Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene 9 : 3689-3694, 1994
7 Mulcahy LS, Gans JH: The fidelity of mouse liver mitochondrial DNA polymerase following long-term administration of carbon tetrachloride, diethylnitrosamine, or phenobarbital. Mol Pharmacol 24 : 329-335, 1983
8 Onishi M, Sokuza Y, Nishikawa T, Mori C, Uwataki K, Honoki K Tsujiuchi T: Different mutation patterns of mitochondrial DNA displacement-loop in hepatocellular carcinomas induced by Nnitrosodiethylamine and a choline-deficient l-amino acid-defined diet in rats. Biochem Biophys Res Commun 362 : 183-187, 2007   DOI   ScienceOn
9 Peng CH, Huang CN, Wang CJ: The anti-tumor effect and mechanisms of action of penta-acetyl geniposide. Curr Cancer Drug Targets 5 : 299-305, 2005   DOI
10 Tan RX, Zheng WF, Tang HQ: Biologically active substances from the genus Artemisia. Planta Med 64 : 295-302, 1998   DOI
11 Volkmann M, Hofmann WJ, M$\ddot{u}$ller M, R$\ddot{a}$th U, Otto G, Zentgraf H, Galle PR: p53 overexpression is frequent in European hepatocellular carcinoma and largely independent of the codon 249 hot spot mutation. Oncogene 9(1) : 195-204, 1994
12 Yoshikawa T, Murakami M, Yoshida N, Seto O, Kondo M: Effects of superoxide dismutase and catalase on disseminated intravascular coagulation in rats. Thromb Haemost 50 : 869-872, 1983
13 Deng HL, Zhang JT: Anti-lipid peroxilative effect of ginsenoside Rb1 and Rg1. Chin Med J 104(5) : 395-398, 1991   ScienceOn
14 Chu CY, Tseng TH, Hwang JM, Chou FP, Wang CJ: Protective effects of capillarisin on tertbutylhydro-peroxide-induced oxidative damage in rat primary hepatocytes. Arch Toxicol 73 :263-268, 1999   DOI   ScienceOn
15 Greenblatt MS, Hollstein M, Harris CC: Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res 54 : 4855-4878, 1994
16 Luo H, Lin S, Ren F, Wu L, Chen L, Sun Y: Antioxidant and antimicrobial capacity of Chinese medicinal herb extracts in raw sheep meat. J Food Prot 70 : 1440-1445, 2007   DOI
17 Matsuda H, Tomohiro N, Hiraba K, Harima S, Ko S, Matsuo K, Yoshikawa M, Kubo M: Study on anti-Oketsu activity of rhubarb II. Anti-allergic effects of stilbene components from Rhei undulati Rhizoma. Biol Pharm Bull 24 : 264-267, 2001   DOI   ScienceOn
18 Mori H, Xu QA, Sakamoto O, Uesugi Y, Ono Y, Koda A, Nishioka I: Immunological mechanisms of antitumor activity of some kinds of Chinese herbs: Meth A-induced delayed type hypersensitivity. Jpn J Pharmacol 48 : 37-46, 1988   DOI
19 Tamano S, Merlino GT, Ward JM: Rapid development of hepatic tumors in transforming growth factor alpha transgenic mice associated with increased cell proliferation in precancerous hepatocellular lesions initiated by N-nitrosodiethylamine and promoted by phenobarbital. Carcinogenesis 15(9) : 1791-1798, 1994   DOI   ScienceOn
20 Gutteridge JM, Beard AP, Quinlan GJ: Superoxide-dependent lipid peroxidation. Problems with the use of catalase as a specific probe for fenton-derived hydroxyl radicals. Biochem Biophys Res Commun 117 : 901-907, 1983   DOI   ScienceOn
21 Kimura Y, Okuda H, Arichi S: Effects of geniposide isolated from Gardenia jasminoides on metabolic alterations in high sugar diet-fed rats. Chem Pharm Bull 30 : 4444-4447, 1982   DOI   ScienceOn
22 Dragan YP, Hully JR, Nakamura J, Mass MJ, Swenberg JA, Pitot HC: Biochemical events during initiation of rat hepatocarcinogenesis. Carcinogenesis 15 : 1451-1458, 1994   DOI   ScienceOn
23 Hinds PW, Finlay CA, Quartin RS, Baker SJ, Fearon ER, Vogelstein B, Levine AJ: Mutant p53 DNA clones from human colon carcinomas cooperate with ras in transforming primary rat cells: a comparison of the 'hot spot' mutant phenotypes. Cell Growth Differ 1 : 571-580, 1990
24 Li X, Zhou XP, Guan YS, Wang YX: Magnetic resonance imaging of hepatocellular carcinoma induced by diethylnitrosamine in Sprague-Dawley rats. Hepatobiliary Pancreat Dis Int 4 : 427-432, 2005
25 Tessitore L, Pani P, Dianzani MU: Mechanisms of the enhanced liver carcinogenesis by choline in female rats: delay in liver growth after partial hepatectomy and stimulation of 2-AAF mitoinhibition. Carcinogenesis 13(10) : 1929-1932, 1992   DOI   ScienceOn
26 Watanabe S, Horie Y, Suzuki A: Hepatocyte-specific Pten-deficient mice as a novel model for nonalcoholic steatohepatitis and hepatocellular carcinoma. Hepatol Res 33 : 161-166, 2005   DOI   ScienceOn
27 Seol HC, Suzuki M, Ohnishi-Kameyama M, Oh MJ, Kim HR, Kim JH, Nagata T: Extraction and identification of antioxidant components from Artemisia capillaris herba. Plant Foods for Human Nutrition 58 : 1-12, 2003
28 Eliyahu D, Raz A, Gruss P, Givol D, Oren M: Participation of p53 cellular tumour antigen in transformation of normal embryonic cells. Nature 312 : 646-649, 1984   DOI   ScienceOn
29 Hu YQ, Tan RX, Chu MY, Zhou J: Apoptosis in human hepatoma cell line SMMC-7721 induced by water-soluble macromolecular components of Artemisia capillaris Thunberg. Jpn J Cancer Res 91 : 113-117, 2000   DOI   ScienceOn
30 Jin H, Sakaida I, Tsuchiya M, Okita K: Herbal medicine Rhei rhizome prevents liver fibrosis in rat liver cirrhosis induced by a choline-deficient L-amino acid-defined diet. Life Sci 76 : 2805-2816, 2005   DOI   ScienceOn
31 Yamahara J, Yamada T, Kimura H, Sawada T, Fujimura H: Biologically active principles of crude drugs. II. Anti-allergic principles in 'Shoseiryu-To' anti inflammatory properties of paeoniflorin and its derivatives. J Pharmacobiodyn 5 : 921-929, 1982   DOI
32 Scherer E, Emmelot P: Kinetics of induction and growth of precancerous liver-cell foci, and liver tumor formation by diethylnitrosamine in the rat. Eur J Cancer 11 : 689-696, 1975   DOI
33 Kus I, Ogeturk M, Oner H, Sahin S, Yekeler H, Sarsilmaz M: Protective effects of melatonin against carbon tetrachloride-induced hepatotoxicity in rats: a light microscopic and biochemical study. Cell Biochem Funct 23 : 169-174, 2005   DOI   ScienceOn
34 Kiso Y, Ogasawara S, Hirota K, Watanabe N, Oshima Y, Konno C, Hikino H: Antihepatotoxic principles of Artemisia capillaris buds. Planta Med 50 : 81-85, 1984   DOI
35 Farber E: The multistep nature of cancer development. Cancer Res 44 : 4217-4223, 1984   ScienceOn
36 Yamahara J, Kobayashi G, Matsuda H, Katayama T, Fujimura H: The effect of scoparone, a coumarin derivative isolated from the Chinese crude drug Artemisiae capillaris flos, on the heart. Chem Pharm Bull 37 : 1297-1299, 1989   DOI   ScienceOn