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The Study to Re-establish the Amount and Major Pollution Source of Wastewater from Seasonal Swine Farms (양돈농가에서의 계절별 세정수 발생량 및 주요 오염원 재설정 연구)

  • Kwag, J.H.;Choi, D.Y.;Kim, J.H.;Jeong, K.H.;Cho, S.H.;Jeong, M.S.;Kang, H.S.;Ra, C.S.
    • Journal of Animal Environmental Science
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    • v.15 no.3
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    • pp.225-230
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    • 2009
  • The purpose of this study was to determine the effect of re-establish the amount and major pollution source of swine wastewater from swine farms. The results obtained in this study was summarized as follow; The quantity of wastewater produced from the average volume of swine wastewater was $2.49{\ell}$/head/day and $2.49{\ell}$ in spring, $2.65{\ell}$ in summer, $2.47{\ell}$ in autumn, and $2.35{\ell}$ in winter. The quantity of wastewater production during summer season was higher than of the other seasons. The water pollutant concentration in wastewater of swine farms, $BOD_5$, SS, T-N and T-P, was $4,689\;mg/{\ell}$, $2,122\;mg/{\ell}$, $3,238\;mg/{\ell}$ and $118\;mg/{\ell}$, respectively.

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Antibacterial Activity of Korean Fig (Ficus carica L.) against Food Poisoning Bacteria (국내산 무화과의 식중독균에 대한 항균활성)

  • Jeong Mi-Ran;Cha Jeong-Dan;Lee Young-Eun
    • Korean journal of food and cookery science
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    • v.21 no.1 s.85
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    • pp.84-93
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    • 2005
  • The antibacterial activities of methanol extract and systematic solvent fractions( -hexane, chloroform, ethyl acetate, and butanol) from Korean common type figs at different ripening stages were tested by the broth dilution method against 8 representative food-poisoning bacteria- : L. monocytogenes, S. aureus, S. enteritidis, E. coli O157:H7, E. coli, Y. enterocolitica, V. parahaemolyticus, and S. typhimurium. The methanol extracts of unripened I and II showed stronger activity than that of the ripened figs especially against L. monocytogenes, S. enteritidis, E. coli O157:H7, Y. parahaemolyticus and S. typhimurium in 10 mg/mL. The systematic solvent fractions showed stronger antibacterial activities than the methanol extract, even al the lower concentrations. The hexane fraction of ripened figs showed higher growth inhibition than those of unripened I and II against L. monocytogenes, E. coli O157:H7, Y. enterocolitica and V. parahaemolyticus. The chloroform fraction showed strong antibacterial activity in all ripening stages against E. coli O157:H7 and V. parahaemolyticus. The butanol fraction showed better inhibition activity in unripened I and II than in the ripened figs. The hexane and chloroform fractions showed inhibition activity of more than $75\%$ against E. coli O157:H7, V. parahaemolyticus in 0.5 mg/mL. Each fraction showed a little different antibacterial activity according to the ripening stages of the fruits and the tested strains. Especially, figs in the unripened II stage revealed superior activity relatively and the hexane and chloroform fractions revealed the strongest activity, followed by the butanol fraction, while the ethylacetate and water fractions hardly showed any activity.

Estimating Grain Weight and Grain Nitrogen Content with Temperature, Solar Radiation and Growth Traits During Grain-Filling Period in Rice (등숙기 온도 및 일사량과 생육형질을 이용한 벼 종실중 및 종실질소함량 추정)

  • Lee, Chung-Kuen;Kim, Jun-Hwan;Son, Ji-Young;Yoon, Young-Hwan;Seo, Jong-Ho;Kwon, Young-Up;Shin, Jin-Chul;Lee, Byun-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.4
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    • pp.275-283
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    • 2010
  • This experiment was conducted to construct process models to estimate grain weight (GW) and grain nitrogen content (GN) in rice. A model was developed to describe the dynamic pattern of GW and GN during grain-filling period considering their relationships with temperature, solar radiation and growth traits such as LAI, shoot dry-weight, shoot nitrogen content, grain number during grain filling. Firstly, maximum grain weight (GWmax) and maximum grain nitrogen content (GNmax) equation was formulated in relation to Accumulated effective temperature (AET) ${\times}$ Accumulated radiation (AR) using boundary line analysis. Secondly, GW and GN equation were created by relating the difference between GW and GWmax and the difference between GN and GNmax, respectively, with growth traits. Considering the statistics such as coefficient of determination and relative root mean square of error and number of predictor variables, appropriate models for GW and GN were selected. Model for GW includes GWmax determined by AET ${\times}$ AR, shoot dry weight and grain number per unit land area as predictor variables while model for GN includes GNmax determined by AET ${\times}$ AR, shoot N content and grain number per unit land area. These models could explain the variations of GW and GN caused not only by variations of temperature and solar radiation but also by variations of growth traits due to different sowing date, nitrogen fertilization amount and row spacing with relatively high accuracy.

Biological activity in hot water extract from fermented Coix lacryma-jobi L. var. mayuen Stapf. by Hericium erinaceum (Bull. : Fr.) (노루궁뎅이균사발효 율무 열수추출물의 유용성분 및 생리활성)

  • Yun, Kyeong-Won;Kim, Kyung-Je;Jin, Seong-Woo;Koh, Young-Woo;Im, Seung-Bin;Ha, Neul-I;Jeong, Hee-Gyeong;Jeong, Sang-Wook;Seo, Kyoung-Sun
    • Journal of Mushroom
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    • v.18 no.1
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    • pp.20-28
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    • 2020
  • This study was conducted to determine the antioxidant, nitrite scavenging, melanin tyrosinase inhibitory, and melanogenesis inhibitory activities of fermented Coix lacryma-jobi L. var. mayuen Stapf. by Hericium erinaceum (Bull.: Fr.) mycelial hot water extract (FCLHE). Additionally, we analyzed β-glucan and ergosterol contents in FCLHE and C. lacryma-jobi hot water extract (CLHE). The ergosterol and β-glucan contents in FCLHE were 740.2 mg% and 245.3 mg%, respectively, whereas these components were not detected in CLHE. FCLHE showed higher cell viability than CLHE. When B16F10 cells were treated with 500 ㎍/mL each of CLHE and FCLHE, the FCLHE treated cells produced 8.9 uM nitric oxide (NO), which was lower than that produced by CLHE treated cells (10.6 uM). The FCLHE treated cells showed significantly greater tyrosinase inhibition and melanin production at all tested concentrations than when compared to the CLHE treated group. Antioxidant parameters such as DPPH and ABTS radical scavenging activities were higher in FCLHE than in CLHE. These results suggest that FCLHE can be used as a raw material for functional foods, for food additives, and in the cosmetic industry.

Isolation and Structure Identification of Photosensitizer from Perilla frutescens Leaves Which Induces Apoptosis in U937 (들깻잎(Perilla frutescens)으로부터 U937 세포에 apoptosis를 유도하는 광과민성 물질의 분리 및 구조동정)

  • Ha, Jun Young;Kim, Mi Kyeong;Lee, Jun Young;Choi, Eun Bi;Hong, Chang Oh;Lee, Byong Won;Bae, Chang Hwan;Kim, Keun Ki
    • Journal of Life Science
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    • v.25 no.1
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    • pp.53-61
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    • 2015
  • In this study, we tried to separate the photosensitizer that induces apoptosis of leukemia cells (U937) from perilla leaves. Perilla leaves (Perilla frutescens Britt var. japonica Hara) are a popular vegetable in Korea, being rich in vitamins (A and E), GABA, and minerals. Dried perilla leaves were extracted with methanol to separate the photosensitizer by various chromatographic techniques. The structure of the isolated compound (PL9443) was identified by 1D-NMR, 2D-NMR, and FAB-mass spectroscopy. Absorbance of the UV-Vis spectrum was highest at 410 nm and was confirmed by the 330, 410, and 668 nm. PL9443 compound was determined to be pheophorbide, an ethyl ester having a molecular weight of 620. It was identified as a derivative compound of pheophorbide structure when magnesium comes away from a porphyrin ring. Observation of morphological changes in U937 cells following cell death induced by treated PL9443 compound revealed representative phenomena of apoptosis only in light irradiation conditions (apoptotic body, vesicle formation). Results from examining the cytotoxicity of PL9443 substance against U937 cells showed that inhibition rates of the cell growth were 99.9% with the concentration of 0.32 nM PL9443. Also, the caspase-3/7 activity was 99% against U937 cells with the concentration of 0.08 nM of PL9443 substance. The result of the electrophoresis was that a DNA ladder was formed by the PL9443. The PL9443 compound is a promising lead compound as a photosensitizer for photodynamic therapy of cancer.

Effects of Topping Time and Split Fertilization on Growth and Root Yield of Scutellaria baicalensis G. (적심시기(摘芯時期)와 분시방법(分施方法)이 황금(黃芩)의 생육(生育) 및 근수량(根收量)에 미치는 영향(影響))

  • Kim, Myeong-Seok;Chung, Byeong-Jun;Park, Gyu-Chul;Park, Tae-Dong;Kim, Sang-Chul;Shim, Jae-Han
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.4
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    • pp.271-276
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    • 1998
  • This experiment was carried out to determine the effects of topping time and fertilizer split application on the growth and root yield of Scutellaria baicalensis G. In case of stem cutting at 25cm above the soil surface early in July and middle in August, plant height was reduced by 37cm compared with nontreatment, but stem diameter, the number of branch per plant and the growth of root increased. Topping treatment twice produced the highest dry root yield (182kg/10a) of all, 15% higher than nontreatment. When nitrogen and potassium were applied by the basal dressing with 60% and by the top dressing early in July and middle in August with 20% in each time, the growth of plant (both top and root) increased. The dry root yield per 10a in top dressing twice was 12% higher than once.

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Effect of Soil Textures and Fertilizer Application Conditions on the Growth, Yield and Quality of Scutellaria baicalensis G. (토성(土性) 및 시비조건(施肥條件)이 황금(黃芩)의 생육(生育), 수량(收量) 및 품질(品質)에 미치는 영향(影響))

  • Kim, Myung-Seok;Park, Jang-Hyun;Chung, Byung-Jun;Park, Gyu-Chul;Park, Tae-Dong;Kim, Sang-Chul;Shim, Jae-Han
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.2
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    • pp.91-98
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    • 2001
  • Scutellaria baicalensis G. was cultivated in plots of different soil textures which were sand loam(SL), loam(L) and clay loam(CL). Also three fertilizer conditions were used; non application (NA)treatment, rice straw manure(RSM) treatment and N-P-K, chemical fertilization(CF) treatment. The chemical-physical properties, such as organic matter, available phosphate, $K_2O$, CaO, clay contents and porosity ratio in CL plot with RSM application were the most proper in CL plot and RSM application for the culture of S. baicalensis plants. RSM had very high contents of total nitrogen, 2.25% and C/N ratios, 21.4. Thus the growth of shoot and root in loam plot with RSM treatment were greater compared to that of CL plot with NA treatment. Whereas, The highest baicalin, baicalein and wogonin contents in roots were found in CL plot with RSM treatment. There was significantly positive correlation between aerial and underground parts of plant, yield and contents of T-N, $K_2O$ but negatively correlated with the contents of baicalin, baicalein and wogonin in S. baicalensis roots.

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Comparison of Physico-Chemical Properties between Waxy and Non-waxy Wheat Grains (찰성밀과 보통밀간의 이화학적 특성 비교)

  • Lee Choon-Ki;Nam Jung-Hyun;Kang Moon-Seok;Ku Bon-Chol;Park Kwang-Keun;Kim Jae-Cheol;Son Young-Koo;Park Jeong-Hwa;Lee Yeong-Ho;Son Jong-Rok;Min Young-Kyoo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.419-427
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    • 2005
  • For the purpose to verify the physico­chemical properties of Korean waxy wheat, comparative analyses between waxy wheat lines and their respective maternal parents were performed on mixing and pasting properties, and flour particle sizes. The particle sizes of waxy wheat flour were significantly larger than those of their parents when milled in a same condition. Although the protein contents of flour in waxy wheat lines tested were high as much as those of bread wheat, the quality parameters showed lower baking uses based on sedimentation volumes and mixing characteristics. Waxy flour required more water than non-waxy flour to obtain the proper mixogram. Waxy wheat flour showed more or less higher onset pasting temperatures and much higher breakdown viscosities than their respective parent flour in the Rapid Viscograph test. Moreover, peak viscosity temperatures and final viscosities were dramatically reduced in waxy wheat lines by showing $79.4 - 81.7^{\circ}C$ and 101 ­116.9 RVU, respectively, compared to their parents in that the temperatures above $95^{\circ}C$ and the viscosity ranges of 148 -171.8 RVU.

Comparison of Grain and Milling Properties between Waxy and Non-waxy Wheat (찰성밀과 보통밀간의 종실 및 제분특성비교)

  • Lee Choon-Ki;Nam Jung-Hyun;Kang Moon-Seok;Ku Bon-Chol;Park Kwang-Keun;Kim Jae-Cheol;Son Young-Koo;Park Jeong-Hwa;Lee Yeong-Ho;Son Jong-Rok;Min Young-Kyoo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.428-434
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    • 2005
  • For the purpose to verify the grain and evaluate milling properties of Korean waxy wheat, com­parison analysis between waxy wheat lines and their respective maternal parents were performed. The waxy lines showed various grain yields of 4.76${\~}$5.79 t/ha depending on parentages, which were corresponding to $80{\~}96\%$ levels of their respective maternal parents. One thousand grain weights of waxy lines were also lighter than its respective part in its parentage by exhibiting 32.8${\~}$34.6 g compared to 32.9${\~}$45.2 g of their parents. Test weights of waxy lines and their parents were 720${\~}$798 g/l and 786${\~}$797 g/l, respectively. The proportions of the grains above 2.5 mm in width were higher in order of Keumgang, Olgeuru, Geuru, SW97134, Suwon 292, Woori, SW97105, and SW97110. Waxy lines exhibited low milling properties by showing the straight flour yields ranging from $61.8\%$ to $67.1\%$ compared with the yields of their parents ranging from $66.1\%$ to $72.5\%$; the waxy lines were significantly lower in first break flour (Bl) and first reduction flour (Rl) yields in the Buller test mill, while significantly higher in the yields of second and third reduction flour (R2 and R3) than the respective ones of their parent wheat.

Effect of Sink and Source Related Characteristics on Grain Weight and Grain Nitrogen Content in Rice (Sink와 Source 관련형질 변이가 벼 종실중 및 종실질소함량 변이에 미치는 영향)

  • Lee, Chung-Kuen;Kwon, Young-Up;Lee, Jae-Eun;Seo, Jong-Ho;Shin, Jin-Chul;Lee, Byun-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.1
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    • pp.45-54
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    • 2009
  • This experiment was conducted to clarify the effects of source and sink related characteristics on grain weight increase and grain nitrogen accumulation in rice. Source was modified by leaf removing treatment with no sink modification in 2002 and sink and source were modified at the same time by different sowing dates, N fertilization rates, row spacings, and thinning treatments at heading in 2007. Grain weight (GW) and grain assimilates produced by photosynthesis during grain-filling ($GW_P$) increased with the increase of source, while the grain assimilates retranslocated from leaf and stem ($GW_T$) decreased. Among the source-related characters, shoot dry weight were most closely related with GW. GW was dependant on $GW_P$ rather than on $GW_T$. Grain nitrogen content (GN) and grain nitrogen absorbed from soil during grain-filling ($GN_S$) increased with the increase of source, while the grain nitrogen retranslocated from leaf and stem ($GN_T$) decreased. Shoot nitrogen content among the source-related characters was related most closely with GN. The contribution of $GN_T$ to GN was relatively large although GN depended more largely on $GN_S$ than $GN_T$. In addition, GN was supplied firstly from $GN_S$ or from $GN_S$ and $GN_T$ at the same time.