• ALGAE
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• 제37권2호
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• pp.149-161
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• 2022

Noctiluca scintillans is a heterotrophic dinoflagellate that causes red-colored oceans during the day (red tides) and glowing oceans at night (bioluminescence). This species feeds on diverse prey, including phytoplankton, heterotrophic protists, and eggs of metazoans. Thus, many scientists have conducted studies on the ecophysiology of this species. It is easy to cultivate N. scintillans at a scale of <1 L, but it is difficult to cultivate them at a scale of >100 L because N. scintillans cells usually stay near the surface, while prey cells stay below the surface in large water tanks. To obtain mass-cultured N. scintillans cells, we developed an automatic system for cultivating N. scintillans on a scale of 100 L. The system consisted of four tanks containing fresh nutrients, the chlorophyte Dunaliella salina as prey, N. scintillans for growth, and N. scintillans for storage, respectively. The light intensities supporting the high growth rates of D. salina and N. scintillans were 300 and 20 µmol photons m^-2 s^-1, respectively. Twenty liters of D. salina culture from the prey culture tank were transferred to the predator culture tank, and subsequently 20 L of nutrients from the nutrient tank were transferred to the prey culture tank every 2 d. When the volume of N. scintillans in the predator culture tank reached 90 L 6 d later, 70 L of the culture were transferred to the predator storage tank. To prevent N. scintillans cells from being separated from D. salina cells in the predator culture tank, the culture was mixed using an air pump, a sparger, and a stirrer. The highest abundance of N. scintillans in the predator culture tank was 45 cells mL^-1, which was more than twice the highest abundance when this dinoflagellate was cultivated manually. This automatic system supplies 100 L of N. scintillans pure culture with a high density every 10 d for diverse experiments on N. scintillans.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.321-325
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• 2004

국내 충적층의 철, 망간 문제는 지속적으로 지적되어오던 수질항목이다. 충적층 지하수 내 철과 망간의 용존 특성을 확인하고자 강변여과수 부지에서 지하수의 수리지화학적 특성을 조사하였다. Fe와 Mn에 의해 오염된 관정은 D-2와 DS-3로 모두 3월에 채취한 시료이며 NO$_3$-N는 모두 0 m/L 이다. NO$_3$-N에 대해 다른 관정은 2 mg/L NO$_3$-N를 넘고 있으며, DS-8와 DS-3에서 11.30과 20.2 mg/L NO$_3$-N의 값으로 먹는물 수질기준을 초과하고 있다. Mn에 대해 오염된 관정은 SJ-3이다. 10월에 채취한 시료에서 DS-2+l8 m에서 채취한 시료가 1.16 mg/L인 것을 제외하고 대부분의 시료가 2 mg/L를 초과하고 있고, DS-6+l3 m과 SJ-3＋10 m에서 채취한 시료가 각각 10.71과 10.31 mg/L NO$_3$-N로 먹는물 수질기준을 초과하고 있다. DO 검층 자료와 NO$_3$-N 농도를 이용하여 Fe와 Mn이 먹는물 수질 기준을 초과하는 D-2와 DS-2 관정의 지하수 체의 혐기성 상태임을 확인하였다. 강변여과수 지역은 전반적으로 NO$_3$-N에 대해 인위적인 오염이 발생하고 있으며, 혐기성 상태 구간이 존재함에 따라서 Fe와 Mn이 먹는물 수질 기준을 초과하여 용존 상태로 존재한다.

• Communications of the Korean Mathematical Society
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• 제12권2호
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• pp.311-324
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• 1997

We show that if $f \in L^{\infty}(D^n)$ satisfies Sf = rf for some r in the unit circle, where S is any convex combination of the iterations of Berezin operator, then f is n-harmonic. And we give some remarks and a conjecture on the space $M_2={f \in L^2(D^2, m \times m)\midBf = f$.

• International Journal of Control, Automation, and Systems
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• 제3권3호
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• pp.430-443
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• 2005

A general construction algorithm of the Grassmann space parameters in linear systems - so-called, the Plucker matrix, 'L' in m-input, p-output, n-th order static output feedback systems and the Plucker matrix, $'L^{aug}'$ in augmented (m+d)-input, (p+d)-output, (n+d)-th order static output feedback systems - is presented for numerical checking of necessary conditions of complete static and complete minimum d-th order dynamic output feedback pole-assignments, respectively, and also for discernment of deterministic computation condition of their pole-assignable real solutions. Through the construction of L, it is shown that certain generically pole-assignable strictly proper mp > n system is actually none pole-assignable over any (real and complex) output feedbacks, by intrinsic rank deficiency of some submatrix of L. And it is also concretely illustrated that this none pole-assignable mp > n system by static output feedback can be arbitrary pole-assignable system via minimum d-th order dynamic output feedback, which is constructed by deterministic computation under full­rank of some submatrix of $L^{aug}$.

• Communications of the Korean Mathematical Society
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• 제32권3호
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• pp.779-787
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• 2017

Let m be the Lebesgue measure on ${\mathbb{C}}$ normalized to $m(D)=1,{\mu}$ be an invariant measure on D defined by $d_{\mu}(z)=(1-{\mid}z{\mid}^2)^{-2}dm(z)$. For $f{\in}L^1(D^n,m{\times}{\cdots}{\times}m)$, Bf the Berezin transform of f is defined by, $$(Bf)(z_1,{\ldots},z_n)={\displaystyle\smashmargin{2}{\int\nolimits_D}{\cdots}{\int\nolimits_D}}f({\varphi}_{z_1}(x_1),{\ldots},{\varphi}_{z_n}(x_n))dm(x_1){\cdots}dm(x_n)$$. We prove that if $f{\in}L^1(D^2,{\mu}{\times}{\mu})$ is radial and satisfies ${\int}{\int_{D^2}}fd{\mu}{\times}d{\mu}=0$, then for every bounded radial function ${\ell}$ on $D^2$ we have $$\lim_{n{\rightarrow}{\infty}}{\displaystyle\smashmargin{2}{\int\int\nolimits_{D^2}}}(B^nf)(z,w){\ell}(z,w)d{\mu}(z)d{\mu}(w)=0$$. Then, using the above property we prove n-harmonicity of bounded function which is invariant under the Berezin transform. And we show the same results for the weighted the Berezin transform in the polydisc.

• YAKHAK HOEJI
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• 제41권6호
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• pp.698-703
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• 1997

Recently, l-deprenyl (selegiline), a relative new antiparkinson`s drug, has been marketed in Korea. As its metabolites, l-methamphetamine and l-amphetamine, are the enantiomers of illicit drugs,d-methamphetamine and d-amphetamine, a method for analysis of enantiomers of methamphetamine and amphetamine in rat urine was investigated. The optical isomers of methamphetamine and amphetamine were analyzed with the chiral derivatizing reagent (S)-(-)-N-(trifluoroacetyl)-prolyl chloride (l-TFP), which was used to form the diastereomers of methamphetamine and amphetamine. And all diastereomers (l-TFP -l-AM, lTFP-d-AM, l-TFP-l-MA & l-TFP-d-MA) were well resolved by capillary gas chromatography. After administration of 10mg/kg l-deprenyl to rat, l-methamphetamine and l-amphetamine were detected without autoracemization to the d form in all urine samples collected during 24hrs, and the ratios of l-amphetamine/l-methamphetamine were 1.1~3.3. l-Amphetamine was detected in only 3 out of 8 urine samples collected during 24~48hrs where as no l-methamphetamine was detected in all cases.

• Imaging Science in Dentistry
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• 제31권1호
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• pp.1-7
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• 2001

Purpose : To evaluate the usefulness of the reconstructed 3-dimensional image on the personal computer in comparison with that of the CT workstation by quantitative comparison and analysis. Materials and Methods : The spiral CT data obtained from 27 persons were transferred from the CT workstation to a personal computer, and they were reconstructed as 3-dimensional image on the personal computer using V-works 2.0/sup TM/. One observer obtained the 14 measurements on the reconstructed 3-dimensional image on both the CT workstation and the personal computer. Paired Nest was used to evaluate the intraobserver difference and the mean value of the each measurement on the CT workstation and the personal computer. Pearson correlation analysis and % incongruence were also performed. Results: I-Gn, N-Gn, N-A, N-Ns, B-A, and G-Op did not show any statistically significant difference (p>0.05), B-O, B-N, Eu-Eu, Zy-Zy, Biw, D-D, Orbrd R, and L had statistically significant difference (p<0.05), but the mean values of the differences of all measurements were below 2 mm, except for D-D. The value of correlation coefficient y was greater than 0.95 at I-Gn, N-Gn, N-A, N-Ns, B-A, B-N, G-Op, Eu-Eu, Zy-Zy, and Biw, and it was 0.75 at B-O, 0.78 at D-D, and 0.82 at both Orbrd Rand L. The % incongruence was below 4% at I-Gn, N-Gn, N-A, N-Ns, B-A, B-N, G-Op, Eu-Eu, Zy-Zy, and Biw, and 7.18%, 10.78%, 4.97%, 5.89% at B-O, D-D, Orbrd Rand L respectively. Conclusion : It can be considered that the utilization of the personal computer has great usefulness in reconstruction of the 3-dimensional image when it comes to the economics, accessibility and convenience, except for thin bones and the landmarks which are difficult to be located.

• Journal of Korean Neurosurgical Society
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• 제58권4호
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• pp.368-372
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• 2015

Objective : The occurrence of acute cerebral infection following deep brain stimulation (DBS) is currently being reported with elevation of C-reactive protein (CRP) level. The aim of the present study was to establish normal range of the magnitude and time-course of CRP increases following routine DBS procedures in the absence of clinical and laboratory signs of infection. Methods : A retrospective evaluation of serial changes of plasma CRP levels in 46 patients undergoing bilateral, two-staged DBS was performed. Because DBS was performed as a two-staged procedure involving; implantation of lead and internal pulse generator (IPG), CRP was measured preoperatively and postoperatively every 2 days until normalization of CRP (post-lead implantation day 2 and 4, post-IPG implantation day 2, 4, and 6). Results : Compared with preoperative CRP levels ($0.12{\pm}0.17mg/dL$, n=46), mean CRP levels were significantly elevated after lead insertion day 2 and 4 ($1.68{\pm}1.83mg/dL$, n=46 and $0.76{\pm}0.38mg/dL$, n=16, respectively, p<0.001). The mean CRP levels at post-lead implantation day 2 were further elevated at post-IPG implantation day 2 ($3.41{\pm}2.56mg/dL$, n=46, respectively, p<0.01). This elevation in post-IPG day 2 rapidly declined in day 4 ($1.24{\pm}1.29mg/dL$, n=46, p<0.05) and normalized to preoperative value at day 6 ($0.42{\pm}0.33mg/dL$, n=46, p>0.05). Mean CRP levels after IPG implantation were significantly higher in patients whose IPGs were implanted at post-lead day 3 than those at post-lead day 5-6 ($3.99{\pm}2.80mg/dL$, n=30, and $2.31{\pm}1.56mg/dL$, n=16, respectively, p<0.05). However, there was no difference in post-IPG day 2 and 4 between them (p>0.05). Conclusion : The mean postoperative CRP levels were highest on post-IPG insertion day 2 and decreased rapidly, returning to the normal range on post-IPG implantation day 6. The duration of post-lead implantation period influenced the magnitude of CRP elevation at post-IPG insertion day 2. Information about the normal response of CRP following DBS could help to avoid unnecessary diagnostic and therapeutic efforts.

• Journal of the Korean earth science society
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• 제34권1호
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• pp.81-92
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• 2013

The purpose of this study was to investigate the communication structures that science gifted students used in small group activities, and to examine the relationship between communication styles and their achievement level. Eight small groups,5 members in each, participated in small group activities, in which they discussed how to calculate the average density of the earth. The communication structures and the achievement level presented in the group activities were analyzed using Pajek, Ucinet 6.0. As a result, we classified the communication styles of science gifted students into monopolistic type and co-ownership type according to the degree of dispersion of the interaction. We also classified it into $D_H{\cdot}N_H$ type, $D_H{\cdot}N_L$ type, $D_L{\cdot}N_H$ type, and $D_L{\cdot}N_L$ type based on the density and network centralization of interaction. The achievement levels of gifted students in their group work were affected by the density of interaction and the network centralization in small group activities, not by the dispersion of interaction among the members of the groups. Therefore, we recommend that teachers make the communication relevant to solving problem when they utilize a small group activity in science teaching.

• Journal of the Society of Cosmetic Scientists of Korea
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• 제35권1호
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• pp.73-78
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• 2009

In order to investigate the potential of a Dayflower (Commelina communis L.) extract as an active in gredient for whitening cosmetics, we prepared aqueous Commelina communis L. extract We measured its mushroom tyrosinase inhibitory activity, cellular tyrosinase activity, and melanin synthesis inhibitory activity in B16 melanoma cells. It did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity. In a melanin synthesis inhibition assay using mouse B16-F10 melanoma cell, it suppressed melanin production up to 32% at a concentration of $1,000{\mu}/mL$ without cytotoxicity, and also reduced cellular tyrosinase activity to above 50 % above the concentration of $250{\mu}g/mL$. In study on the melanogenic protein expressions, it had especially influence on expression of tyrosinase protein, which is a well-known key protein on melanogenesis, and tyrosinase expression was gradually decreased in a dose-dependent. Dayflower also blocked N-glycosylation of TRP-2, but affected on the expression of TRP-1 rather than on blocking of N-glycosylation processing. Therefore, this result suggests that aqueous Commelina communis L. extract could be used as an active ingredient for whitening cosmetics.