• 제목/요약/키워드: Mycelial Growth

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Excellent strain selection and optimal mycelial growth condition of Pleurotus cornucopiae (노랑느타리벗섯(Pleurotus cornucopiae)의 우수균주 선발 및 최적 균사배양)

  • Jang, In-Ja;Chung, Ki-Chul;Chang, Hyun-You
    • Journal of Mushroom
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    • 제3권1호
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    • pp.40-44
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    • 2005
  • The results of examining selection of optimal mycelial growth condition excellent strain for determining the condition of Pleurotus cornucopiae are as follows. 1) Mycelial growth and density of P. cornucopiae were the highest in the medium of MCM(87.8mm/10d) followed by the order of PDA, YM, MEA and Czapek. 2) Optimal temperature for the mycelial growth and density of P. cornucopiae was shown to be $25^{\circ}C$, but the hyphae were dead at $5^{\circ}C$. The mycelial growth and density of KNAC2003 strain was the highest at $25^{\circ}C$(87.8mm/10d) followed by the order of 25, 30, 20, 35, 10 and $5^{\circ}C$. 3) Optimal pH for the mycelial growth and density was revealed to be 6.0(88.3mm/10d), but the mycelial growth and density were shown to be retarded in above or below pH 6.0.

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Cultural Characteristics for the Enhanced Mycelial Growth of Ramaria botrytis

  • Lee, Tae-Hee;Han, Yeong-Hwan
    • Mycobiology
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    • 제33권1호
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    • pp.12-14
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    • 2005
  • The culture conditions for the enhanced mycelial growth of Ramaria botrytis was investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and 5.0, respectively. It was shown that starch was best of several carbon sources in Czapek-Dox medium as a minimal medium for the enhanced mycelial growth. Organic nitrogen sources were better than inorganic ones for mycelial growth. The appropriate vitamin and mineral salt were biotin and FeCl3, respectively. When this strain was cultured with $FeCl_3$ for 30 days, 19.23 g/l of dry mycelium of R. botrytis was obtained.

Pigment Production and Color Diference of Liquid Beni-koji under Submerged Cultural Conditions (액체 홍국의 배양조건에 따른 색소생산과 색조의 변화)

  • 강성국;정순택
    • Microbiology and Biotechnology Letters
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    • 제23권4호
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    • pp.472-478
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    • 1995
  • Mycelial growth, color difference and productivity of red pigment of beni-koji by Monascus anka KCCM 11832 were examined with respect to it's pigment in submerged culture with various medium and culture conditions. Shaking incubation was more promoted mycelial growth and the production of pigments than that for non-shaking incubation, and red color became ten times deeper. The production of red pigment was the highest when incubated at 25$\circ$C for 7 days in pH 6.0, but mycelial growth was showed the highest at 32.5$\circ$C. The levels of carbon and nitrogen source for maximum red pigment production were 2% rice powder and 0.05% peptone, respectively and the level of peptone for maximum pigment production was lower than that for maximum mycelial growth. Among pigmentation promoting agents tested, MgSO$_{4}$, was found to be suitable for the production of red pigment, and the optimum level was 0.1%.

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Culture conditions for mycelial growth of Poria cocos

  • Jo, Woo-Sik;Park, Ju-Ri;Oh, So-Ra;Kang, Min-Gu;Kim, Woo-Hyun;Park, Seung-Chun
    • Journal of Mushroom
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    • 제14권1호
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    • pp.6-13
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    • 2016
  • This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and $35^{\circ}C$, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $FeSO_4{\cdot}7H_2O$ as mineral salts.

Effect of Dry Heat Treatment of Red Ginseng and Red Ginseng Residue on Mycelial Growth and on Induced Tolerance of Fusarium oxysporum to Mercury Chloride (홍미삼과 홍삼정박의 건열처리가 Fusarium owsporum의 균사 생장과 승홍에 대한 내성에 미치는 영향)

  • Kim, Yeong-Ho;Park, Myeong-Han;Lee, Jong-Won
    • Journal of Ginseng Research
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    • 제16권2호
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    • pp.99-104
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    • 1992
  • Korean red ginseng and water extract residue of red ginseng roots were treated with dry heat and incorporated in PDA medium to examine the effect of the materials on induced tolerance against mercury chloride and mycelial growth of Fusarium oxysporum. Ginseng residue was not effective in the inducement of tolerance to mercury chloride regardless of dry heat treatment. However, the heat treatment of ginseng and ginseng residues stimulated the mycelial growth of the fungus. The materials responsible for the detoxification appeared to be water-soluble. The stimulation of the fungal mycelial growth on the media by the heat treatment was highest in the water extract of ginseng. Due to the heat treatment, the mycelial growth was also slightly increased in n-hexane and methanol extracts of ginseng, compared with the ginseng fractions without dry heat treatment.

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Effect of Water Potential on Mycelial Growth and Sporangial Formation by Pythium spp. (Water Potential이 Pythium속(屬) 균(菌)의 균사(菌絲) 신장(伸長)과 Sporangia 형성(形成)에 미치는 효과(效果))

  • Sung, Jae-Mo
    • The Korean Journal of Mycology
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    • 제11권3호
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    • pp.105-108
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    • 1983
  • The mycelial growth of Pythium graminicola, P. ultimum, P. rostratum was best at -3 osmotic potential. P. monospermum was best grown at -8 bars osmotic potential. Mycelial growth by Pythium spp. was prevented at -42 bars for P. graminicola, P. ultimum and P. monospermum. Sporangial formation by P. graminicola and P. ultimum was influenced by water potential in much the same way as mycelial growth: -3 bars was maximum and -42 bars or below was minimal. Pythium spp. tested was grown faster on PDA than other media including oat meal agar. P. ultimum was best grown on any media among Pythium spp. tested.

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Optimal Conditions for the Mycelial Growth of Coprinus comatus Strains

  • Jang, Myoung-Jun;Lee, Yun-Hae;Liu, Jun-Jie;Ju, Young-Cheol
    • Mycobiology
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    • 제37권2호
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    • pp.103-108
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    • 2009
  • The principal objective of this study was to acquire basic data regarding the mycelial growth characteristics for the artificial cultivation of Coprinus comatus. 12 URP primers were employed to evaluate the genetic relationships of C. comatus, and the results were divided into three groups. Among six kinds of mushroom media, MYP medium was selected as the most favorable culture medium for C. comatus. The optimal temperature and pH ranges for the mycelial growth of C. comatus were $23{\sim}26^{\circ}C$ and pH 6${\sim}$8, respectively. The carbon and nitrogen sources for optimal mycelial growth were sucrose and tryptone, respectively.

Optimization of Cultural Conditions for Mycelial Growth and Exo-Polysaccharide Production in Jar Fermentation by Fomitopsis pinicola

  • Cha, Wol-Suk;Jilu, Ding;Lee, Choon-Beom;Nam, Hyung-Geun;Lee, Jun-Han;Maeng, Jeung-Moo;Lim, Hwan-Hee
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2005년도 생물공학의 동향(XVI)
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    • pp.187-191
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    • 2005
  • The Study was carried out to investigate in the optimal mycelial growth and Exo-Polysaccharides of Fomitopsis pinicola. Jar fermentations were carried out to optimize the culture conditions for mycelial growth and exo- polysaccharide production. The optimal agitation speed and aeration rate were 200 rpm and 1.5 v.v.m., respectively. Under optimal culture conditions, the maximum mycelial growth and exo-polysaccharide production after 11 days with a 5 L jar fermenter containing the optimized medium were 10.21 g/L and 3.56 g/L, respectively. However, the fundamental information obtained this study is insufficient in the development of a efficient process for mycelial growth and exe-polysaccharide production from Fomitopsis pinicola.

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Studies on the nutrients sources of Pleurotus cornucopiae (노랑느타리버섯(Pleurotus cornucopiae)의 영양원에 관한 연구)

  • Jang, In-Ja;Chung, Ki-Chul;Chang, Hyun-You
    • Journal of Mushroom
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    • 제3권2호
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    • pp.75-78
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    • 2005
  • The results of studies for determining the nutrients sources of mycelial growth for optimal condition of Pleurotus cornucopiae are as follows; 1) Optimal carbon source of mycelial growth of Pleurotus cornucopiae is maltose(357mg/25ml/15d); 2) Optimal nitrogen source of mycelial growth of Pleurotus cornucopiae is peptone(347mg/25ml/15d); 3) Optimal organic acid source of mycelial growth of Pleurotus cornucopiae is glutamic acid(389mg/25ml/15d); 4) Optimal vitamin source of mycelial growth of Pleurotus cornucopiae is biotin(399mg/15d); and 5) Optimal C/N ratio for mycelial growth for determining the condition of Pleurotus cornucopiae is 40(398mg/15d).

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Optimal Condition of Mycelial Growth of Grifola frondosa (잎새버섯 균주의 균사체 생육 최적조건)

  • Chi, Jeong-Hyun;Kim, Jeong-Han;Won, Sun-Yee;Seo, Geon-Sik;Ju, Young-Cheoul
    • The Korean Journal of Mycology
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    • 제35권2호
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    • pp.76-80
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    • 2007
  • This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and investigated its optimal culture condition. Among four kinds of mushroom culture media, PDA medium was selected as the suitable culture medium. The optimal conditions for the mycelial growth of G. frondosa in PDA medium were $25^{\circ}C$ and $4{\sim}5$ of pH, respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively, and the highest mycelial growth was observed when the C/N ratio was $10{\sim}20$.