• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권4호
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• pp.271-281
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• 2004

Adenoid cystic carcinoma is malignant tumor in salivary gland, and its behavior is very invasive. Of all malignant tumor adenoid cystic carcinoma is occured in frequency of 4.4% in major salivary gland, and 1.29% in minor salivary gland. Histopathologically, adenoid cystic carcinoma is characterized by a cribriform appearance, and tubular form and solid nest type tumor can be seen. The tumor cell structure composed of modified myoepithelial cell, and basaloid cell. Extracellular matrix of this tumor cell contains variable ground substance with basement membrane component. Basement membrane matrix composed of collagen fibers, glycoproteins, proteoglycans, and its function is well known that it participate in differentiation, proliferation, and growth of tumor cell. Basement membrane molecule is essential for invasion of peripheral nerve, blood vessel, skeletal muscle in tumor cell of adenoid cystic carcinoma. In many studies, the tumor cell of adenoid cystic carcinoma containing modified myoepithelial cell participate in synthesis of proteoglycan. In this study, tissue sample of adenoid cystic carcinoma of human salivary gland were obtained from 15 surgical specimen, and all specimen were routinely fixed in 10% formalin and embedded. Serial $4-{\mu}m$ thick sections were cut from paraffin blocks. the histopathologic evaluation was done with light microscopy. And, the immunohistochemical staining, characteristics of glycosaminoglycan were observed. For biochemical analysis of glycosaminoglycan, isolation of crude glycosaminoglycan from tumor tissue and Western bolt analysis were carried out. With transmission electomicroscopy, tumor cell were observed. Biologic behavior of adenoid cystic carcinoma was observed with distribution and expression of basement membrane of glycosaminoglycan in tumor cells, The results obtained were as follows: 1. In immunohistochemical study, chondroitin sulfate is postively stained in tumor cell and interstitial space, dermatan sulfate is weakly stained in ductal cell. But keratan sulfate is negatively stained. 2. In immunohistochemical study, heparan sulfate is strong positive stained in tumor cell and basement membrane, especially in invasion area to peripheral nerve tissue. 3. In transmission electromicroscpic view, the tumor cells are composed modifed myoepithelial cells, and contains many microvilli and rough endoplasmic reticulum. 4. In Western blot analysis, the expression of glycosaminoglycan is expressed mostly in heparan sulfate. From the results obtained in this study, tumor cell of adenoid cystic carcinoma is composed modified myoepithelial cell, and glycosaminoglycan of basement membrane molecule of heparan sulfate and chondroitin sulfate mostly participate in the development and invasiveness of adenoid cystic carcinoma by immunohistochemical study and western blot analysis.

• Restorative Dentistry and Endodontics
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• 제34권6호
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• pp.537-550
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• 2009

본 연구에서는 Porphyromonas endodontalis와 죽상경화증 및 심혈관질환과의 관계를 알아보기 위해, P. endodontalis가 사람의 관상동맥 내피세포에 침투했을 때 나타나는 유전자 발현의 변화를 microarray와 real-time PCR로 측정하였고, 발현이 증가된 유전자 중에서 죽상경화증과 연관된 유전자들이 관련 KEGG pathway 상에서 유의성 있는 영향을 미치는지를 분석하여 다음과 같은 결과를 얻었다. 1. Porphyromonas endodontalis는 사람의 관상동맥 내피세포에 침투하였다. 2. Microarray 분석결과, 대조군보다 발현이 2배 이상 증가된 유전자는 625개였고, 1/2이하로 감소된 유전자는 154개였다. 3. 발현이 2배 이상 증가된 유전자 중에는 염증성 cytokine 및 chemokine, 세포자멸사, 혈액응고와 면역반응 관련 유전자들이 포함되었다. 4. Microarray 분석결과를 확인하기 위해 발현이 2배 이상 증가된 유전자 중에서 10개의 유전자를 선택하여 real-time PCR을 시행하였고, 그 결과 microarray에서와 마찬가지로 발현 정도가 대조군보다 모두 높게 나타났다. 따라서 P. endodontalis가 사람의 관상동맥 내피세포에 만성적으로 작용했을 때, 심혈관질환에서 중요한 부분을 차지하는 죽상경화증을 유발하는 위험 요소 중의 하나로 작용할 수 있을 것으로 판단된다. 이와 관련된 자세한 기전을 이해하기 위해서는 앞으로 더 많은 연구가 필요할 것으로 보인다.

• 한국수산과학회지
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• 제6권3_4호
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• pp.112-122
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• 1973

붕어와 금붕어에 기생하는 사상충, Philometroides carassii (Ishii, 1931)를 구제할 목적으로 1971년 4월부터 1972년 12월까지 당년어 붕어 200마리와 금붕어 150마리에 이 충의 유충을 여러가지 방법으로 감염시켜, 생활사를 규명하고 약물치료 실험을 하여 다음과 같은 결과를 얻었다. 1. 이 충은 Cyclops sp.가 중간숙주이며 이것의 복강에 침입한 유충은 3시간 이후에 감염력을 가졌다. 2. Cyclops sp.를 경유하지 않고 직접 먹힌 유충은 어체내에서 24시간내 장에서 소화되거나 배설되었다. 3. 중간숙주를 경유하여 어체의 장에 기생한 유충은 $1\~10$일후 복강속으로 침입하고, 여기서 성장하여 미성숙성충이 되었다. 붕어 복강속에서 기생하는 미성숙 성충은 $18\~20$개월 후 꼬리지느러미로 나와 성충이 되었다. 금붕어 복강속에 기생하는 미성숙 성충은 $7\~8$개월 후 꼬리지느러미로 나와 성충이 되었다. 4. 어체 복강속에 기생하는 미성숙 성중에서 본 종의 수컷을 최초로 발견했으며 암컷보다 대형이었다. 5. 성충에 대한 약물 치료효과는 기대할 수 없었고 성충에서 방출되는 유충에 대해 약욕 치료실험한 결과 dipterex가 가장 효과가 있었으며 4ppm으로 5일만에 대부분 폐사되었다.

• 대한세포병리학회지
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• 제7권2호
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• pp.144-150
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• 1996

Although bladder cancers are very common, little is known about their molecular pathogenesis. It is known that p53 alteration is found in about 60% of muscle-invasive bladder cancer, necessiating aggressive therapy and poor outcome. We examined the nuclear expression of p53 protein, using D07 monoclonal antibody in the urine samples from 31 patients with transitional cell carcinoma of the bladder to investigate the correlation of p53 overexpression with histologic grades and depth of invasion. The positive rate of p53 protein was 27% in superficial bladder tumor, but increased up to 71% in the invasive bladder carcinomas. The overexpression of p53 protein increased according to Mostofi grading system from 18% in grade I, 45% in grade II, and up to 100% in grade III. The p53 expression tended to be higher in the invasive and high grade bladder cancers than in the superficial and low grade ones(p<0.05). These results suggest that immunohistochemical analysis of the urine specimen in the bladder cancer patients could be a useful method of screening for the presence of p53 mutant protein. The mutant p53 protein expression may be an indicator of bladder cancer with more proliferative potential and/or aggressive biologic behavior.

• 대한한의학방제학회지
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• 제15권2호
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• pp.47-64
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• 2007

This report describes 94 prescriptions related to the use of Radix Angelicae Dahuricae main bl ended from Donguibogam. The following conclusions were reached through investigations on the prescriptions that use Radix Angelicae Dahuricae as a key component. 11.7% of wind. 10.6% of carbuncle and deep-rooted carbuncle. recorded the largest number of clinical frequency of the prescriptions in therapeutic use when Radix Angelicae Dahuricae was ta ken as a monarch drug in prescriptions, Prescriptions that utilize Radix Angelicae Dahuricae as t he main component are used in the treatment of apoplexy and carbuncle and deep-rooted carbuncle. headache. wound and they are also used for treating 26 different types of diseases. The prescriptions are compounded with Radix Angelicae Dahuricae as a monarch drug can ap ply to apoplexy, exogenous febrile disease, invasion by wind. wind-cold pathogen, wind-heat path ogen. epidemic disease, pestilence. bruise, bites, deficiency of liver and kidney, deficiency, phlegm-fire, phlegm-heat. The dosage of Radix Angelicae Dahuricae is 0.37g to 7.5g, however 3.75g has be en taken the most for clinical application. The function of Radix Angelicae Dahuricae is to expelling Wind and relieving pain, to expelling wound and forming muscle. to astrict and neutralizing poison. to expelling wind and to getting through body hole. to emit and relieving pain from the combination of drugs and prescriptions.

• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1539-1543
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• 2015

Background: Uroplakins have been widely investigated as potential markers in patients with bladder cancer because these proteins are specific to the urothelium. However, the role of uroplakin proteins in bladder cancer remains unknown. In this study, preoperative serum levels of uroplakin III were measured in patients with urothelial carcinoma of the urinary bladder and examined for possible association with clinicopathological features and clinical outcomes. Materials and Methods: This study included 52 bladder cancer patients at various stages and 28 healthy controls. Uroplakin III levels were detected in preoperative sera using an automated dot blot system and a micro-dot blot array. Results: There was a significant increase in serum uroplakin III levels in patients with bladder cancer as compared to healthy controls (p<0.05). In addition, serum uroplakin III levels were associated with muscle-invasive status, high grade and lymphovascular invasion (p<0.02). Log-rank tests indicated high serum uroplakin III to be significantly associated with cancer-specific mortality. Conclusions: Determination of serum uroplakin III level could be valuable for identifying patients with biologically aggressive bladder cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.1135-1138
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• 2015

Background: The objective of this study was to investigate the MSCT characteristics of PTL in order to enhance the awareness of this uncommon entity among both clinicians and radiologists. Materials and Methods: The clinicopathological data and MSCT images of 27 patients with PTL were retrospectively reviewed. The MSCT appearances were classified into three types: type 1, solitary nodule surrounded by normal thyroid tissue; type 2, multiple nodules in the thyroid, and type 3, enlarged thyroid glands with a reduced attenuation with or without peripheral thin hyperattenuating thyroid tissue. Results: The patients were enrolled in the study with a mean age of 68 years (range, 51-86years) and compression symptoms or enlarged cervical lymph nodes at diagnosis. Hashimoto's thyroiditis was in 20 patients. All patients had non-Hodgkin lymphoma of B-cell in origin, including 22 cases of diffuse large B-cell lymphoma (DLBCL) and 5 of low-grade B-cell lymphoma of mucosa-associated lymphoid tissue (MALT). For MSCT appearance, type 1 pattern was observed in 2 patients, type 2 in 8, and seventeen type 3 in 17. The lesions occurred in more than one lobe with a mean maximal transverse diameter of 6.9 cm and an ill-defined margin. Most tumors showed a homogeneous attenuation equal to that of surrounding muscles before contrast and obvious enhancement after contrast. Cervical lymph node involvement and invasion of the trahea and (or) esophagus were mainly observed in patients with DLBCL. Conclusions: PTL should be clinically considered in elder patients presenting with a history of Hashimoto's thyroiditis and cervical lymphadenopathy. The MSCT characteristics of PTL includes a mass diffusely affecting more than one thyroid lobe, isointense to muscle and obvious enhancement before and after contrast. DLBCL, the most common histological subtype of PTL, is associated with a higher invasive tendency.

• Childhood Kidney Diseases
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• 제18권2호
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• pp.116-122
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• 2014

신장 농양은 요로감염에 의한 신장 감염의 가장 심한 상태로 신주위로 파열되어 신장주위농양으로 진행할 수 있으며, 신장 흉터의 후유증을 남길 수 있다. 대개 항생제 치료가 지연되거나 적절한 항생제를 선택하지 못했을 때 발생할 수 있으며, 소아에서는 해부학적 요로계 기형을 동반하기도 한다. 저자들은 방광요관역류가 동반된 대장균에 의한 요로감염 환아에서 발열 초기에 민감한 항생제로 치료하였음에도 불구하고, 좌측 요근과 신피막 주위의 침윤을 동반한 신장 농양으로 진행하여 장기간의 항생제와 함께 경피적 흡인으로 치료된 1례와 해부학적 기형 없이 2주간 발열이 있었던 환아에서 포도알균에 의한 비장과 횡경막을 침범한 다격벽의 신장 농양이 확인되어 항생제 치료와 함께 도관삽입 후 배농하여 치료하였으나 이후 추적관찰에서 신장 흉터가 발생하였던 1례를 경험하였기에 이를 보고 하는 바이다.

• 한국어병학회지
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• 제3권1호
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• pp.1-9
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• 1990

새우 양식장(養殖場)에서 Vibrio병(病)으로 여겨지는 보리새우에서 분리(分離)한 6균주(菌株)를 검토(檢討)한 결과(結果) Vibrio sp.로 동정(同定)되었다. 분리균주(分離菌株)는 모두 같은 특징(特徵)을 나타내었으며 같은 군(群)으로 추정(推定)되었다. 분리균(分離菌)으로 보리새우에 재감염(再感染)시킨 결과(結果) 양식장(養殖場)의 병(病)든 새우와 같은 병리학적(病理學的) 변화(變化)를 나타내었다.

• 대한세포병리학회지
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• 제19권2호
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• pp.136-143
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• 2008

Urothelial carcinoma accounts for 90% of all the cases of bladder cancer. Although many cases can be easily managed by local excision, urothelial carcinoma rather frequently recurs, tends to progress to muscle invasion, and requires regular follow-ups. Urine cytology is a main approach for the follow-up of bladder tumors. It is noninvasive, but it has low sensitivity of around 50% with using the conventional cytospin preparation. Liquid-based cytology (LBC) has been developed as a replacement for the conventional technique. We compared the cytomorphometric parameters of $ThinPrep^{(R)}$ and cytospin preparation urine cytology to see whether there are definite differences between the two methods and which technique allows malignant cells to be more effectively discriminated from benign cells. The nuclear-to-cytoplasmic ratio value, as measured by digital image analysis, was efficient for differentiating malignant and benign urothelial cells, and this was irrespective of the preparation method and the tumor grade. Neither the $ThinPrep^{(R)}$ nor the conventional preparation cytology was definitely superior for distinguishing malignant cells from benign cells by cytomorphometric analysis of the adequately preserved cells. However, the $ThinPrep^{(R)}$ preparation showed significant advantages when considering the better preservation and cellularity with a clear background.