• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2013.05a
• /
• pp.585-586
• /
• 2013

• Applied Chemistry for Engineering
• /
• v.33 no.5
• /
• pp.451-458
• /
• 2022

Semiconductor quantum dots (QDs) are optical probes with excellent fluorescence properties. Therefore, they have been applied to various bio-medical imaging techniques and biosensors. Due to the unique optical characteristics of wide absorption and narrow fluorescence energy bands, multiple types of signals can be generated by the combination of fluorescence wavelengths from different QDs, which enables the simultaneous detection of more than two biomarkers. In this review, the advantages and applications of QDs and QD nanobeads (QBs) in multiple biomarker assays were described, and new developments or improvements in multiplexed biomarker detection techniques were summarized. In particular, recent reports were summarized, focusing on the design strategies in immunoassay construction and signal transducing materials for fluorescence-linked immunosorbent assays using QDs and immunochromatographic assays using QBs. New detection platforms will be developed for early diagnosis of diseases and other fields if multiplexed detection technologies of excellent accuracy and sensitivity are combined with artificial intelligence algorithms.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.12A
• /
• pp.1279-1284
• /
• 2004

In this paper, we propose a novel and simple optical technique for the detection of subcarrier-multiplexed(SCM) labels using optical interleavers. Optical-baseband packet signals with suppressed subcarriers appear at the through-pass port of the optical interleaver and SCM labels with suppressed optical carrier exit from the optical SCM extraction port. Since it does not require optical circulators, this structure shows less insertion loss than the previously proposed optical label detectors. The periodic nature of the interleaver transfer function makes it possible to detect multiple SCM channels simultaneously from an incoming wavelength-multiplexed signal stream. Detection of a 155-Mb/s ASK modulated 9.79-GHz subcarrier using a 10-GHz SCM optical label detector has been performed successfully and verified through optical spectra and bi t-error-rate measurements.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.1
• /
• pp.221-226
• /
• 2012

DNA-functionalized silver and silver/gold bimetallic nanoparticle superstructure probes with controllable sizes and optical properties are synthesized using monothiol DNA and dithiothreitol. The superstructures exhibit a very narrow size distribution, which can be easily controlled by balancing the ratio of dithiothreitol and DNA. These superstructures assemble reversibly in a highly cooperative manner, and are SERS active. Multiplexed colorimetric detection of DNA targets using these superstructure probes has been demonstrated to identify three different DNA target sequences that are associated with three lethal diseases, respectively.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.9
• /
• pp.1323-1329
• /
• 2021

Micro-scale magnetic beads are widely used for isolation of proteins, DNA, and cells, leading to the development of in vitro diagnostics. Efficient isolation of target biomolecules is one of the keys to developing a simple and rapid point-of-care diagnostic. A zinc finger protein (ZFP) is a double-stranded (ds) DNA-binding domain, providing a useful scaffold for direct reading of the sequence information. Here, we utilized two engineered ZFPs (Stx2-268 and SEB-435) to detect the Shiga toxin (stx2) gene and the staphylococcal enterotoxin B (seb) gene present in foodborne pathogens, Escherichia coli O157 and Staphylococcus aureus, respectively. Engineered ZFPs are immobilized on a paramagnetic bead as a detection platform to efficiently isolate the target dsDNA-ZFP bound complex. The small paramagnetic beads provide a high surface area to volume ratio, allowing more ZFPs to be immobilized on the beads, which leads to increased target DNA detection. The fluorescence signal was measured upon ZFP binding to fluorophore-labeled target dsDNA. In this study, our system provided a detection limit of ≤ 60 fmol and demonstrated high specificity with multiplexing capability, suggesting a potential for development into a simple and reliable diagnostic for detecting multiple pathogens without target amplification.

• Annals of Laboratory Medicine
• /
• v.38 no.6
• /
• pp.569-577
• /
• 2018

Background: The increasing prevalence of drug-resistant tuberculosis (TB) infection represents a global public health emergency. We evaluated the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform [QMAP], QuantaMatrix, Seoul, Korea) to rapidly identify the Mycobacterium tuberculosis complex (MTBC) and detect rifampicin (RIF) and isoniazid (INH) resistance-associated mutations. Methods: A total of 200 clinical isolates from respiratory samples were used. Phenotypic anti-TB drug susceptibility testing (DST) results were compared with those of the QMAP system, reverse blot hybridization (REBA) MTB-MDR assay, and gene sequencing analysis. Results: Compared with the phenotypic DST results, the sensitivity and specificity of the QMAP system were 96.4% (106/110; 95% confidence interval [CI] 0.9072-0.9888) and 80.0% (72/90; 95% CI 0.7052-0.8705), respectively, for RIF resistance and 75.0% (108/144; 95% CI 0.6731-0.8139) and 96.4% (54/56; 95% CI 0.8718-0.9972), respectively, for INH resistance. The agreement rates between the QMAP system and REBA MTB-MDR assay for RIF and INH resistance detection were 97.6% (121/124; 95% CI 0.9282-0.9949) and 99.1% (109/110; 95% CI 0.9453-1.0000), respectively. Comparison between the QMAP system and gene sequencing analysis showed an overall agreement of 100% for RIF resistance (110/110; 95% CI 0.9711-1.0000) and INH resistance (124/124; 95% CI 0.9743-1.0000). Conclusions: The QMAP system may serve as a useful screening method for identifying and accurately discriminating MTBC from non-tuberculous mycobacteria, as well as determining RIF- and INH-resistant MTB strains.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.33 no.9C
• /
• pp.734-742
• /
• 2008

In spatially multiplexed MIMO systems that enable high data rate transmission over wireless communication channels, the spatial demultiplexing at the receiver is a challenging task and various demultiplexing methods have been developed. Among the previous methods, maximum likelihood detection with QR decomposition and M-algorithm (QRM-MLD), sphere decoding (SD), QOC, and MOC schemes have been reported to achieve a (near) maximum likelihood (ML) hard decision performance. In general, however, the reliability of soft output of these schemes is not satisfactory. In this paper, we propose a method which enhances the reliability of soft output. By computer simulations, we demonstrate the improved performance by the proposed method.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.1C
• /
• pp.43-50
• /
• 2007

In the paper, we address QRM-MLD (Maximum Likelihood Detection with QR Decomposition and M-algorithm) signal detection method for spatially multiplexed MIMO (Multiple Input Multiple Output) systems. Recently, the QRM-MLD signal detection method which can achieve 1Gbps transmission speed for next generation mobile communication was implemented in a MIMO testbed for the mobile moving at a pedestrian speed. In the paper, we propose a novel signal detection method 'reduced complexity QRM-MLD' that achieves identical error performance as the QRM-MLD while reducing the computational complexity significantly. We rigorously compare the two detection methods in terms of computational complexity to show the complexity reduction of the proposed method. We also perform a set of computer simulations to demonstrate that two detection methods achieve identical error performance.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.7C
• /
• pp.616-626
• /
• 2007

In spatially multiplexed MIMO systems that enable high data rate transmission over wireless communication channels, the spatial demultiplexing at the receiver is a challenging task, and various demultiplexing methods have been developed recently by many researchers. Among the previous methods, maximum likelihood detection with QR decomposition and M-algorithm (QRM-MM)), and sphere decoding (SD) schemes have been reported to achieve a (near) maximum likelihood (ML) performance. In this paper, we propose a novel signal detection method that achieves a near ML performance in a computationally efficient manner. The proposed method is demonstrated via a set of computer simulations that the proposed method achieves a near ML performance while requiring a complexity that is comparable to that of the conventional MMSE-OSIC. We also show that the log likelihood ratio (LLR) values for all bits are obtained without additional calculation but as byproduct in the proposed detection method, while in the previous QRM-MLD, SD, additional computation is necessary after the hard decision for LLR calculation.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.33 no.4A
• /
• pp.394-402
• /
• 2008

In this paper, we propose a novel soft output generation method for spatially multiplexed MIMO systems. The receiver complexity of spatially mutiplexed MIMO system is in proportion to the number of candidate vectors. The ML signal detection method considers all possible vectors as candidates, thereby achieving a high performance, however, its complexity is very high. Low complexity receiver techniques involving a small number of candidate vectors, provide soft output values of low reliability. In this paper, we propose a method to improve reliability of the soft output values obtained using a small number of candidate vectors.