Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Recent Progress in Multiplexed Detection of Biomarkers Based on Quantum Dots

양자점 기반 다중 바이오마커 검출법의 연구동향

  • Kim, Yerin (Department of Chemical Engineering, Myongji University) ;
  • Choi, Yu Rim (Department of Chemical Engineering, Myongji University) ;
  • Kim, Bong-Geun (Department of Chemical Engineering, Myongji University) ;
  • Na, Hyon Bin (Department of Chemical Engineering, Myongji University)
  • Received : 2022.09.08
  • Accepted : 2022.09.15
  • Published : 2022.10.10
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Abstract

Semiconductor quantum dots (QDs) are optical probes with excellent fluorescence properties. Therefore, they have been applied to various bio-medical imaging techniques and biosensors. Due to the unique optical characteristics of wide absorption and narrow fluorescence energy bands, multiple types of signals can be generated by the combination of fluorescence wavelengths from different QDs, which enables the simultaneous detection of more than two biomarkers. In this review, the advantages and applications of QDs and QD nanobeads (QBs) in multiple biomarker assays were described, and new developments or improvements in multiplexed biomarker detection techniques were summarized. In particular, recent reports were summarized, focusing on the design strategies in immunoassay construction and signal transducing materials for fluorescence-linked immunosorbent assays using QDs and immunochromatographic assays using QBs. New detection platforms will be developed for early diagnosis of diseases and other fields if multiplexed detection technologies of excellent accuracy and sensitivity are combined with artificial intelligence algorithms.

반도체 양자점은 우수한 형광 특성을 가진 광학 탐침자로 생명-의학 영상화 기술 및 바이오센싱 분야에서 광범위하게 활용되고 있다. 양자점은 넓은 광흡수 에너지띠, 좁은 형광 에너지띠와 같은 광학 특성을 가지므로 서로 다른 형광 파장을 지닌 양자점을 조합해 다종의 신호를 생성할 수 있도록 구성하면 복수의 바이오마커를 동시에 검출할 수 있다. 본 총설에서는 이와 같은 다중 검출 분석법에서의 양자점 및 이에 기반한 양자점 나노비드가 가지는 장점과 활용 사례를 기술하고 다중 형광 바이오마커 검출법의 최근 개발 동향 및 개선사항을 요약 정리하였다. 특히 양자점을 활용한 형광-결합 면역흡착 분석법, 양자점 나노비드를 이용한 면역크로마토그래피 분석법 등 면역 분석법에서의 신호 전환 소재 디자인을 중심으로 최근의 연구 결과를 검토하였다. 정확성과 민감도가 우수한 다중 바이오마커 검출 기술이 확보된 데이터를 처리하고 해석하는 인공지능 알고리즘과 결합될 경우 질병의 조기 진단을 포함한 다양한 분야에 활용가능한 새로운 검출 플랫폼의 개발로 이어질 것으로 기대된다.

Keywords

Acknowledgement

이 성과는 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2021R1F1A1061247).

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