• Biomolecules & Therapeutics
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• 제26권3호
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• pp.282-289
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• 2018

Melanin is a pigment produced from tyrosine in melanocytes. Although melanin has a protective role against UVB radiation-induced damage, it is also associated with the development of melanoma and darker skin tone. Tyrosinase is a key enzyme in melanin synthesis, which regulates the rate-limiting step during conversion of tyrosine into DOPA and dopaquinone. To develop effective RNA interference therapeutics, we designed a melanin siRNA pool by applying multiple prediction programs to reduce human tyrosinase levels. First, 272 siRNAs passed the target accessibility evaluation using the RNAxs program. Then we selected 34 siRNA sequences with ${\Delta}G{\geq}-34.6kcal/mol$, i-Score value ${\geq}65$, and siRNA scales score ${\leq}30$. siRNAs were designed as 19-bp RNA duplexes with an asymmetric 3' overhang at the 3' end of the antisense strand. We tested if these siRNAs effectively reduced tyrosinase gene expression using qRT-PCR and found that 17 siRNA sequences were more effective than commercially available siRNA. Three siRNAs further tested showed an effective visual color change in MNT-1 human cells without cytotoxic effects, indicating these sequences are anti-melanogenic. Our study revealed that human tyrosinase siRNAs could be efficiently designed using multiple prediction algorithms.

• 한국건설관리학회논문집
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• 제9권6호
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• pp.268-276
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• 2008

In highway construction projects, concrete pavement productivity has been challenged with constructors and decision-makers; at present there are few methods available to accurately evaluate the factors impacting on it. Any inefficient method to analyze it leads to the excessive schedule, higher rehabilitation costs, shorter service life, and reduction of ride quality. To implement these negative outcomes, constructors or decision-makers need a systematic tool that can be used to categorize the factors related to construction productivity. This paper applies multiple regression technique for productivity analysis of the Jointed Plane Concrete Pavement (JPCP), identifies the significant factors, and provides a predictive model assisting in monitoring and managing the productivity of the JPCP construction process. The completed and progressive projects are employed to derive and assess the proposed model. The results are analyzed to illustrate its capabilities.

• 유통과학연구
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• 제12권6호
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• pp.41-48
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• 2014

Purpose - This study aims to investigate a solution to reduce the negative reactions arising from the performance of duties or the job distress of workers in the service industry, because human resource statistics regarding workers in the service sector have been unreported, while concerns regarding exposure to emotional exertion and poor working conditions have been continuously increasing. Research design, data, and methodology - This study specifically focused on workers in highway service areas. It differs from previous studies because it involves the perspective of the person-organization fit and regards workers' cognition of leaders through the psychological distance toward the leader and the contact frequency between workers and leaders within the framework of the leader-individual fit. Moreover, this study highlights the role of the self-leadership of workers as an important factor that becomes manifested in the individual-level fit to the organization. Hence, this study investigates whether the positive role of the above factors, in turn, could reduce job distress. Workers from highway service areas in Gyeonggi-do province provided data; 141 valid questionnaires are collected. SPSS 19.0 and AMOS 19.0 were used to test the reliability and validity of constructs. Simple regression, multiple regression, and 3 step mediation tests were used to test the hypotheses after the correlation tests. Results - Results indicated that leader-member contact frequency and psychological distance have negative effects on job distress but positive effects on self-leadership. A mediation test revealed that self-leadership, in the relationships between contact frequency and job distress and between psychological distance and job distress, resulted in partial mediation and full mediation, respectively. Conclusions - The result can be understood through two different possible explanations. First, service area workers generally possess a positive perspective toward their leaders. This can be interpreted to mean that increased contact frequency and psychological distance would be considered as supports from the leader, rather than intrusive controls or management schemes. Therefore, the management in highway service areas should invest efforts in increasing contact frequency as well as maximizing psychological similarities by adopting the viewpoints of workers in terms of moral and ethical management, to reduce the workers' job distress. The results relating to self-leadership also indicate that increased contact frequency must be accompanied by intentions for the effective promotion of workers' self-leadership. It also signifies the necessity of a strategic approach by leaders to induce workers to perceive "in-group"ness as suggested both by the similarity-attraction theory and by the social identity theory. In addition, the results of the mediation test of self-leadership indicate that because workers' self-leadership activates upon increased contact frequency by the leader, it should not be a means of control and should not be utilized only from the perspective of management. It is also suggested that strategies such as transfer of authority could have a positive effect in promoting the expansion of self-influence from workers.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.598-609
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• 2017

Pediococcus pentosaceus ID-7 was isolated from kimchi, a Korean fermented food, and it showed high activity for lactose hydrolysis. The ${\beta}$-galactosidase of P. pentosaceus ID-7 belongs to the GH2 group, which is composed of two distinct proteins. The heterodimeric LacLM type of ${\beta}$-galactosidase found in P. pentosaceus ID-7 consists of two genes partially overlapped, lacL and lacM encoding LacL (72.2 kDa) and LacM (35.4 kDa). In this study, Escherichia coli MM294 was used for the production of LacL, LacM, and LacLM. These three types of recombinant proteins were expressed, purified, and characterized. The specific activities of LacLM and LacL were 339 and 31 U/mg, respectively. However, activity was not detected with LacM alone. The optimal pH of LacLM and LacL was pH 7.5 and pH 7.0, and the optimal temperature of LacLM and LacL was $40^{\circ}C$ and $50^{\circ}C$, respectively. The optimal temperature changes indicate that LacLM is able to achieve higher activity at a relatively lower temperature. LacLM was strongly activated by $Mg^{2+}$, $Mn^{2+}$, and $Zn^{2+}$, which was not true for LacL. Consistent with this, EDTA strongly inactivated LacLM and LacL, but the presence of reducing agents did not dramatically alter the activity. Taken together, multiple alignment of amino acid sequences and phylogenetic analysis results of LacL and LacM of P. pentosaceus ID-7 suggest the evolution of LacL into LacLM and that the use of divalent metal ions results in higher activity.

• 한국의류학회지
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• 제32권7호
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• pp.1137-1148
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• 2008

The purposes of this study were to investigate the differences in clothing consumption behaviors among female consumer groups in their twenties and to understand the type of consumer group pursuing fast fashion. Questionnaire survey was conducted to 230 female consumers residing in Seoul and Kyung Gi area from June 18 to 29 in 2007, and 178 were used for data analysis. Data analysis were conducted with SPSS 12 program on the reliability test, factor analysis, cluster analysis, correlation analysis, ANOVA, Duncan's multiple range test. Factors analyses were employed for the attitude toward fashion and shopping, and shopping motives. Four factors were formulated for the attitude toward fashion: interest in fashion, fashion-orientation, fashion leadership and fashion conformity. Six factors for the attitude toward shopping were found: information searching, shopping enjoyment, store patronage, impulse buying, brand-orientation and convenience. Buying motives for fashion goods were classified into 3 factors: for matching & occasion, to-be-in-fashion and for necessity. Four clusters were identified based on the attitude toward fashion: the fashion-interested, the fashion-indifferent, fashion leaders and the individuality-oriented. Among the groups, significant differences were found in information searching, shopping enjoyment and store patronage. Fashion leaders tended to spend more for expensive and up-to-dated fashion items, and for higher quantity than other groups. Consequently fashion leaders showed attractive customer characteristics for the fast fashion companies.

• 한국프랜차이즈경영연구
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• 제9권4호
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• pp.33-43
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• 2018

Purpose - In hotel industry, quality of leader-member exchange(LMX) relationship is very critical, because it impacts on the employee's work attitude and behaviors. Thus, this research examines the effect of LMX on employee loyalty in the context of hotel business and identifies mediating roles of work stressors, work role performance in the relationship between LMX and employee loyalty. This research suggests the guidelines for how hotel leaders should manage their employees and build employee loyalty that improve management and business performance. Research design, data, and methodology - This study tests the structural relationship between LMX, work stressors, work role performance, and employee loyalty. Work role performance divide into three sub-dimensions such as individual task proficiency, individual task adaptivity, and individual task proactivity. In order to examine the purposes of this research, research model and hypotheses were developed. All constructs were measured with multiple items developed and tested in the previous studies. The data were collected from 113 franchise hotel employees and were analyzed using SPSS 22.0 and SmartPLS 3 program. Result - The findings of this research are as follows. First, leader-member exchange(LMX) have significant positive impacts on work stressors, work role performance, and employee loyalty. Second, work stressors have significant negative impacts on work role performance and employee loyalty. Third, work role performance has significant positive impact on employee loyalty. Conclusions - The outcomes of this research indicate that hotel leaders should focus on the dyadic relationship with their employees how to improve employee productivity through LMX relationship. In turn, the quality of this relationship influences employees's work attitudes and behaviors. As a result of increasing job demands in hotel business which relies heavily on human resources, the hotel leader must find ways to prevent or reduce stressors and associated strains. If hotel employees perceive the high quality of LMX relationship, they improve their work role performance which influences loyalty. Therefore, the hotel leaders should develop monetary or non-monetary reward system for the employees and, make an efforts to have unique social exchange relationships with employees.

• The Journal of Asian Finance, Economics and Business
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• 제7권6호
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• pp.485-494
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• 2020

The study aims to identify and measure factors affecting the salaries of employees in manufacturing enterprises in Hanoi, the important area of Vietnam's economy. We conducted a questionnaire consisting of 31 observation variables with a 5-point Likert scale. Independent variables were measured from 1 "without effect" to 5 "strongly". Based on the literature review and results of interviews, a total of 350 questionnaires were sent to participants; 300 of them met the standards and were subject to be analyzed. The results of Exploratory Factor Analysis (EFA) and Multiple Regression Analysis (MRA) identify six main determinants influencing the salaries of employees in manufacturing enterprises in Hanoi, including Paying views of business leaders (PV), Financial ability of the enterprise (FA), Capacity of workers (CW), Capacity of the contingent of employees engaged in salary work (CC), Role of grassroots trade unions (TU), and State policies and laws on labor - salaries (STL). Based on the findings, some recommendations have been proposed to help the firm leaders design appropriate personnel policies for creating better job satisfactions for employees in the future. On this basis, the authors propose a number of recommendations to improve the salaries of employees in manufacturing enterprises in Hanoi.

• 보건의료산업학회지
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• 제11권2호
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• pp.43-53
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• 2017

Objectives : This study aimed to investigate the effects of resonant leadership and structural empowerment on the job satisfaction of registered nurses working in hospitals. Methods : Data were surveyed from 307 nurses in three general hospitals in Seoul and Gyeonggi-do using self-administered questionnaires from March 15 to 31, 2016. We conducted t-test, ANOVA, Pearson's correlation analysis, and multiple regression analysis using SPSS 21.0 for analysis. Results : Resonant leadership and structural empowerment had significant effect on the job satisfaction of nurses. These two attributes of a quality work environment explained 45.3% and 40.1% of the variance in organizational and opportunity satisfaction while controlling for age, marital status, religion, education, work department, work type, career and work position. Conclusions : The findings of this study suggest that resonant leaders and structural empowerments contribute to nurse job satisfaction. Therefore, developing resonant leadership skills and structural empowerment among nurse leaders in hospitals will ensure the creation of healthy work environments that promote job satisfaction and retention of nurses.

• Asian Journal for Public Opinion Research
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• 제8권3호
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• pp.180-196
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• 2020

This paper considers Japanese political interviews to integrate conversation and facial expression analysis. The behaviors of political leaders will be disclosed by analyzing questions and responses by using the turn-taking system in conversation analysis. Additionally, audiences who cannot understand verbal expressions alone will understand the psychology of political leaders by analyzing their facial expressions. Integral analyses promote understanding of the types of facial and verbal expressions of politicians and their effect on public opinion. Politicians have unique techniques to convince people. If people do not know these techniques and ways of various expressions, they will become confused, and politics may fall into populism as a result. To avoid this, a complete understanding of verbal and non-verbal behaviors is needed. This paper presents two analyses. The first analysis is a qualitative analysis that deals with Prime Minister Shinzō Abe and shows that differences between words and happy facial expressions occur. That result indicates that Abe expresses disgusted facial expressions when faced with the same question from an interviewer. The second is a quantitative multiple regression analysis where the dependent variables are six facial expressions: happy, sad, angry, surprised, scared, and disgusted. The independent variable is when politicians have a threat to face. Political interviews that directly inform audiences are used as a tool by politicians. Those interviews play an important role in modelling public opinion. The audience watches political interviews, and these mold support to the party. Watching political interviews contributes to the decision to support the political party when they vote in a coming election.

• The Journal of Asian Finance, Economics and Business
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• 제8권4호
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• pp.687-697
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• 2021

This study aims to address the prevailing skepticism that conventional leadership models are not effective in the Middle East. With the same logic, selecting leaders is a confusing task in the Middle East due to the haphazardness surrounding this task and the lack of clarity of appropriate selection criteria. This study employs samples of leadership theories and frameworks that were scrutinized to classify specific leadership dimensions. The triangular model stemmed from the previous studies that addressed Leadership in general and the Leadership model for the Middle East. With the use of the literature review methodologies and historical analysis, the leadership model's description will be demonstrated. The results revealed that there are various dimensions of Leadership, especially concerning the Middle East's organizational environment. The findings of this study suggested a new model which assists in clarifying the issue by setting a group of 10 personality traits with eight behavioral skills and social status. Implications of this study enable making Leadership easier in practice. Furthermore, this will enable recognition of Leadership according to specific criteria, which will help make greater equality and empowerment at all levels of the system. This research localizes Leadership and paves the way to automate leaders' selection process with a guarantee of fairness among candidates.