• Food Science and Biotechnology
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• v.14 no.5
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• pp.566-571
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• 2005

Process of screw-pumping system (SPS) was optimized for mass production of encapsulated bifidus. SPS entrapment device was composed of feeding component, with optimized nozzle size and length of 18G (0.91 cm) and 4 mm, respectively, screw pump, and 37-multi-nozzle. Screw component had five wing turns [radius (r)=26 to 15 mm] from top to bottom of axis at 78-degree angle from middle of the screw, and two wings were positioned at screw edge to push materials toward nozzle. For nozzle component, 37 nozzles were attached to 20-mm round plate. Air compressor was attached to SPS to increase productivity of encapsulated bifidus. This system could be operated with highly viscous (more than 300 cp) materials, and productivity was higher than $1128\;{\pm}\;30\;beads/min$. Viability of encapsulated bifidus was $5.45\;{\times}\;10^8\;cfu$/bead, which is superior to that of encapsulated bifidus produced by other methods ($2.51{\times}10^8\;cfu$/bead). Average diameter of produced beads was $2.048\;{\pm}\;0.003\;mm$. Survival rate of SPS-produced encapsulated bifidus was 90% for Simulator of the Human Intestinal Microbial Ecosystem test and 88% in fermented milk (for 14 days). These results show SPS is effective for use in development of economical system for mass production of viable encapsulated bifidus.

• Journal of Korean Society on Water Environment
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• v.22 no.5
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• pp.796-804
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• 2006

The objective of this research was to develop ecological multi-metric models using natural fish assemblages for a diagnosis of current stream health condition, and apply the model to nationwide lotic ecosystems of the Geum River, the Youngsan River, and the Sumjin River. The ecological stream health model was based on the index of biological integrity (IBI), which was originally developed in North American streams by Karr (1981), and the Rapid Bioassessment Protocol (RBP), which was scientifically established by the US EPA (1999). The metric numbers and metric attributes were partially changed for the regional applications, so the scoring criteria was modified for the assessment. Overall, metric values, based on the IBI calculations, reflected conventional water quality characteristics, based on nutrient regime, and agreed with results of staticeco-toxicity tests. Some stations impaired in terms of stream health were identified by the IBI approach, and also major key stressors affecting the stream health were identified by additional evaluations of physical habitats. Our preliminary results suggested that biological integrity in stream ecosystems was largely disturbed by habitat degradation as well as chemical pollutions. This new approach would be used as a key tool for ecological restorations and species conservations in the degraded aquatic ecosystems in Korea and applied for elucidating major causes of ecological disturbances. Ultimately, this approach provides us an effective management strategy of stream ecosystems through establishments of ecological networks in various watersheds.

• Korean Journal of Environment and Ecology
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• v.25 no.3
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• pp.412-420
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• 2011

This study was conducted to quantitatively analyze the temporal change of green space network at multi-scales from 1975 to 2006 with the application of graph theory in Seoul, Korea. Remarkable change of connectivity was detected in green space networks at the scale ranging from 1,000 ~ 1,600 m during 30 years. Green spaces and their networks have been restoring after 1990 since forest areas had been fragmented in 1975. In 2006, we identified the important core habitat areas that can sustain diverse wildlife species and stepping stones composed of small patches that can link these core habitat areas. Green spaces showed high correlation with the relative importance value of green space connectivity. So, this study could graphically represent green space networks of Seoul City. Green spaces of core areas distributed at the northern and southern boundary, and those of stepping stones possessing the high value of betweenness centrality consisted at the middle, eastern and western boundary. These results indicate that green space network can be graphically and quantitatively explained by degree centrality, betweenness centrality and relative importance value of connectivity with the application of graph theory.

• Journal of the Korean Institute of Landscape Architecture
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• v.30 no.3
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• pp.46-56
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• 2002

This landscape design proposal was presented to a design competition for the Culture.Welfare.Administration Complex of Yongin-City which was held by Yongin-City in December, 2001. The site is located at 68-19 Yukbuck-dong, Yongin-City, Kyunggi-do and has an area of 236,449$m^2$. The judging criteria for landscape design set by the Yongin-City could be articulated as follows: an environment friendly design respecting the surrounding environment, a locale as a community center of Yongin-City, a resting place for the welfare of employees and visitors, and finally a place with diverse landscape elements reflecting the individual identity of each facility. This proposal chose the main design concept of this project as‘Nature meets City'. A grove of pine trees fragments the multi building complex, so that‘nature'was brought into the‘city'. A double ground system was introduced to respect the existing topography and to enhance the efficency of the land. This design proposal consists of four segments. Each segment has its own design theme. Outdoor space of this multi-purpose complex was designed with themes of nature, culture, welfare and administration. Space composition with grid and natural looking curvilinear lines was a design motive for this complex. The nature oriented planting design was introduced to respect the existing groves of Mt. Suksung. Specific species were chosen to follow its own spatial character in each space. An environmental sculpture called‘Dream Soaring'was placed in the middle of‘Citizen Plaza'. Its light and feather-looking shape symbolizes the vision of Youngin-City. At night people can watch the diverse color changes on its mirror-like surface.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.458-470
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• 2013

Haliotis discus, a useful abalone of herbivorous gastropod, shows feeding preference to marine algae depending upon their growth stage and recognition of taste. This study was carried out to investigate this abalone's algal preferences and the presence of feeding stimulants. In single-choice experiments the small (S) group generally preferred Ulva pertusa (Chlorophyta), whereas the medium (M) and large (L) group preferred both Laminaria japonica (Phaeophyta) and Undaria pinnatifida (Phaeophyta). In multi-choice experiments using 4 algal species of L. japonica, U. pertusa, U. pinnatifida and Ecklonia cava (Phaeophyta), the results were same as in the single-choice experiments; the S group preferred U. pertusa the most, while the M and L group preferred both U. pinnatifida and L. japonica. However E. cava was not preferred by any groups. In order to examine the presence of feeding stimulant, chemical compounds from algae used as feed were isolated and identified. The abalone responded to water soluble matters of L. japonica, U. pinnatifida and U. pertusa, but those of E. cava and Sargassum sagamianum (Phaeophyta) were not attractive to them. In feeding stimulant experiments using fat soluble matters, the S group preferred the fat soluble matter of U. pertusa the most, while the M group and the L group preferred those of U. pertusa and U. pinnatifida, and those of L. japonica, respectively. However the fat soluble matter of S. sagamianum was not attractive to the abalone. The results of feeding stimulant experiments were same as those of single-choice or multi-choice experiments, which showed that compound lipids in fat soluble matter might act as feeding-stimulant.

• Journal of Korean Society of Rural Planning
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• v.18 no.3
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• pp.149-164
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• 2012

The purpose of the present study is to construct a rural development strategy from the nexus between spatial changes in specialized crops and suitable cultivation area of the crops. This paper pays particular attention to identify product life cycle of specialized crops in rural areas and estimate the impact of climate change on alterations in spatial distribution of the crops. In order to do so, first of all, this study applies multi-level model (Random coefficient model) to estimate the regional coefficient of five orchard crops. It utilizes the data 1995 to 2010 Korea Agricultural Census. Futhermore, it also adopts overlay analysis by ArcGIS to identify the development path of the crops and the relationship with climate change. Based on the results, it suggests a mechanism activating regional agriculture. The findings propose re-searching and relocating specialized regions of the crops. Especially, it proves each rural area can drive the new agricultural strategy to strengthen regional agriculture by estimating the relationship between development of specialized crops and suitable cultivation areas. For instance, shifting specialized crops in particular regions and enriching genetic or species varieties can be primary measures and it will contribute to improve the reliable base for income sources in the rural communities. This paper also offers specific policy implications regarding rural development plans in response to crops' life cycle and climate changes.

• Journal of Microbiology and Biotechnology
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• v.33 no.4
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• pp.500-510
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• 2023

In this study, lactic acid bacteria were isolated from 21 top-selling probiotic products on Korean market and their antimicrobial resistance were analyzed. A total 152 strains were claimed to be contained in these products and 70 isolates belonging to three genera (Bifidobacterium, Lactobacillus, and Lactococcus) were obtained from these products. RAPD-PCR showed diversity among isolates of the same species except for two isolates of Lacticaibacillus rhamnosus from two different products. The agar dilution method and the broth dilution method produced different MICs for several antimicrobials. With the agar dilution method, five isolates (three isolates of Bifidobacterium animalis subsp. lactis, one isolate of B. breve, one isolate of B. longum) were susceptible to all nine antimicrobials and 15 isolates were multi-drug resistant. With the broth microdilution method, only two isolates (one isolate of B. breve and one isolate of B. longum) were susceptible while 16 isolates were multi-drug resistant. In this study, only two AMR genes were detected: 1) lnu(A) in one isolate of clindamycin-susceptible and lincomycin-resistant Limosilactobacillus reuteri; and 2) tet(W) in one tetracycline-susceptible isolate of B. longum B1-1 and two tetracycline-susceptible isolates and three tetracycline resistant isolates of B. animalis subsp. lactis. Transfer of these two genes via conjugation with a filter mating technique was not observed. These results suggest a need to monitor antimicrobial resistance in newly registered probiotics as well as probiotics with a long history of use.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.4
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• pp.37-49
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• 2015

The goal of this research is to examine air temperature changes according to tree type, plantation type, roadside green area structure, and green volume of street green area within a city. The plantation type that could be analyzed for comparison by tree type with over 3 species was 1 rows of tree+shrubs. The results of analysis of average air temperature difference between pedestrian and car streets vis-a-vis 1 row of tree+shrub in high air temperature areas were: Pinus densiflora, $1.35^{\circ}C$; Zelkova serrata, $1.84^{\circ}C$; Ginkgo biloba, $2.00^{\circ}C$; Platanus occidentalis, $2.57^{\circ}C$. This standard large wide canopy species was analyzed by the roadside to provide shade to have a significant impact on air temperature reduction. In terms of analysis of the relationship between plantation type of roadside trees and air temperature, the average air temperature difference for 1 row of tree type was $1.80^{\circ}C$; for 2 rows of trees it was $2.15^{\circ}C$. In terms of analysis of the relationship between the roadside green area structure and air temperature, for tree type, average air temperature $1.94^{\circ}C$: for tree+shrub type, average air temperature $2.49^{\circ}C$; for tree+mid-size tree+shrub type, average air temperature $2.57^{\circ}C$. That is, air temperature reduction was more effective in a multi-layer structure than a single layer structure. In the relationship analysis of green volume and air temperature reduction, the air temperature reduction effect was enlarged as there was a large amount of green volume. There was a relationship with the green volume of the road, the size of the tree and number of tree layers and a multi-layer structured form of planting. The canopy volume was large and there were a great number of rows of the tree layer and the plantation type of multi-layer structure, which is what is meant through a relationship with the green volume along the roadside. Green composition standards for air temperature reduction effects and functional improvement were proposed based on the result. For a pedestrian street width of 3m or less in the field being ideal, deciduous broadleaf trees in which the canopy volume is small and the structure of the tree+shrub type through the greatest 1m green bend were proposed. For a pedestrian street width of over 3m, deciduous broadleaf trees in which the canopy volume is large and is multi-layer planted with green bend over 1m, tree+mid-size tree+shrub type was proposed.

• Journal of Environmental Health Sciences
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• v.33 no.5
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• pp.408-421
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• 2007

The concentration characteristics of atmospheric particle matters (PM) including $PM_{2.5},\;PM_{10}$, and TSP were evaluated through the measurement data of PM_{2.5}$ (fine particulate), PM_{10-2.5}$ (coarse particulate), and PM_{over-10}$ collected using a MCI (multi-nozzle cascade impactor) sampler of a three-stage filter pack in spring of 2006 in Iksan area. During the sampling period of 10-15 March and 24 days from 8 April to 2 May, 32 samples for PM of each size fractions were collected, and then measured for PM mass concentrations and water-soluble inorganic ion species. Average concentrations of $PM_{2.5},\;PM_{10}$, TSP were $57.9{\pm}44.1mg/m^3$, $96.6{\pm}89.1mg/m^3$, and $114.8{\pm}99.7mg/m^3$, respectively. Water-soluble inorganic ion fractions to PM mass were found to be 36.5%, 18.0%, and 11.1% for $PM_{2.5}$, $PM_{10-2.5}$ and $PM_{over-10}$, respectively. By showing the high concentrations of PM samples during Asian dust events, those three fractions of PM were distinguished between the samples of Asian dust event and the samples of no event. However, the increase of PM concentrations observed during Asian dust events showed a different pattern for some Asian dust events. The differences of those three fractions in the size distribution may depend on differences on place of occurrence of Asian dust storm and course of transport from China continent to Iksan area in Korea. However, the extent of PM mass contribution during Asian dust events was generally dominated by the coarse particles rather than the fine fraction of PM. The variations of water-soluble inorganic ion species concentration in those three PM fractions between the samples of Asian dust event and the samples of no event were also discussed in this study.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.197-205
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• 2020

With the purpose of designing companion planting of groundcover plants for ornamental uses, this study identified the yearly growth characteristics of nine species of different life forms, analyzed the coverage characteristics of individual plants, and suggested combinations of plants suitable for each life form. Polygonatum odoratum var. pluriflorum, Liriope platyphylla and Hosta capitata, as short-grained plants that can grow to more than 20 cm, tended to grow for 60 days after planting in April and maintain their shape thereafter. Their aerial parts started to wither and enter dormancy after September. Saxifraga stolonifera, Dianthus chinensis and Sedum middendorffianum tended to continuously grow until September after planting in April and their growth declined after September. Lysimachia nummularia, as a creeping plant that grows creeping on the ground, started to show a rapid growth three months after planting. Sedum sarmentosum grew slowly until August and the aerial parts started to wither from September when the temperature decreases. The coverage characteristics of these nine species that grow differently after companion planting were surveyed and the growth of Sedum sarmentosum showed the highest number of companions. It was found that Hosta capitata can be companion planted with Sedum middendorffianum, Saxifraga stolonifera, and Lysimachia nummularia. These results indicate that among different shoot growth types species propagated with their stems creeping on the ground or those that can grow vegetatively with non-rhizome parts are more suitable for companion planting with others than those of which rhizomes branch.