Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Optimization of Screw Pumping System (SPS) for Mass Production of Entrapped Bifidus

  • Ryu, Ji-Sung (Department of Biological Engineering, Inha University) ;
  • Lee, Yoon-Jong (Department of Biological Engineering, Inha University) ;
  • Choi, Soo-Im (Department of Biological Engineering, Inha University) ;
  • Lee, Jae-Won (Department of Biological Engineering, Inha University) ;
  • Heo, Tae-Ryeon (Department of Biological Engineering, Inha University)
  • Published : 2005.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Process of screw-pumping system (SPS) was optimized for mass production of encapsulated bifidus. SPS entrapment device was composed of feeding component, with optimized nozzle size and length of 18G (0.91 cm) and 4 mm, respectively, screw pump, and 37-multi-nozzle. Screw component had five wing turns [radius (r)=26 to 15 mm] from top to bottom of axis at 78-degree angle from middle of the screw, and two wings were positioned at screw edge to push materials toward nozzle. For nozzle component, 37 nozzles were attached to 20-mm round plate. Air compressor was attached to SPS to increase productivity of encapsulated bifidus. This system could be operated with highly viscous (more than 300 cp) materials, and productivity was higher than $1128\;{\pm}\;30\;beads/min$. Viability of encapsulated bifidus was $5.45\;{\times}\;10^8\;cfu$/bead, which is superior to that of encapsulated bifidus produced by other methods ($2.51{\times}10^8\;cfu$/bead). Average diameter of produced beads was $2.048\;{\pm}\;0.003\;mm$. Survival rate of SPS-produced encapsulated bifidus was 90% for Simulator of the Human Intestinal Microbial Ecosystem test and 88% in fermented milk (for 14 days). These results show SPS is effective for use in development of economical system for mass production of viable encapsulated bifidus.

Keywords

References

  1. Process Biochem. v.26 Effective oxygenation of immobilized cells though reduction in bead diameter: a review Ogbonna, J.C.;Matsumura, M.;Kataoka, H. https://doi.org/10.1016/0032-9592(91)80025-K
  2. Biotechnol. Bioeng. v.49 Microencapsulation of yeast cells and their use as a biocatalyst in organic solvents Green, K.D.;Gill, I.S.;Khan, L.A.;Vilfson, E.N. https://doi.org/10.1002/(SICI)1097-0290(19960305)49:5<535::AID-BIT6>3.0.CO;2-K
  3. J. Phycol. v.33 The use of immobilization in alginate beads for long-term storage of Pseudanabaena galeata (Cyanobacteria) in the laboratory Romo, S.;Perezmartinez, C. https://doi.org/10.1111/j.0022-3646.1997.01073.x
  4. Biotechnol. Bioprocess Eng. v.6 Effect of skim milkalginate beads on survival rate of bifidobacteria Yu, W.K.;Yim, T.B.;Lee, K.Y.;Heo, T.R. https://doi.org/10.1007/BF02931959
  5. Biotechnol. Bioeng. v.42 Membrane formation by interfacial cross-linking of chitosan for microencapsulation of Lactococcus lactics Groboillot, A.F.;Champagne, C.P.;Darling, G.D.;Poncelet, D.;Neufeld, R.J. https://doi.org/10.1002/bit.260421005
  6. Can. Inst. Food Sci. Technol. J. v.22 Survival of microencapsulated Bifidobacterium pseudolongum in simulated gastric and intestinal juice Rao, A.V.;Shiwmarain, N.;Mahmj, I. https://doi.org/10.1016/S0315-5463(89)70426-0
  7. Biotechnol. Bioeng. v.38 Diffusion of lactose in K-carrageenan/locust bean gum gel beads with or without entrapped growing lactic acid bacteria Arnaud, J.P.;Lacroix, C. https://doi.org/10.1002/bit.260380913
  8. Biotechnol. Prog. v.9 Effect of chelatants on gellan gel rheological properties and setting temperature for immobilization of living bifidobacteria Camelin, I.;Lacroix, C.;Paquin, C.;Prevost, H.;Cachon, R.;Divies, C. https://doi.org/10.1021/bp00021a008
  9. Process Biochem. v.31 Influence of the immobilization conditions on the efficiency of $\alpha$-amylase production by Bacillus licheniformis Dobreva, E.;Ivanova, V.;Tonkova, A.;Radulova, E. https://doi.org/10.1016/0032-9592(95)00052-6
  10. Process Biochem. v.30 Immobilization of Bacillus stearothermophilus cells by entrapment in various matrices Manolov, R.J.;Kambourova, M.S.;Emanuilova, E.I. https://doi.org/10.1016/0032-9592(95)95712-R
  11. Biotechnol. Prog. v.13 Production of small, monodispersed alginate beads for cell immobilization Seifert, D.B.;Phillips, J.A. https://doi.org/10.1021/bp9700723
  12. Biotechnol. Bioeng. v.27 A new technique for the production of immobilized biocatalyst in large quantities Hulst, A.C.;Tramper, J.;Reit, K.V.;Westerbeek, M.W. https://doi.org/10.1002/bit.260270617
  13. Ind. Eng. Chem. Res. v.31 Formation of uniform liquid drops by application of vibration to laminar jets Haas, P.A. https://doi.org/10.1021/ie00003a043
  14. Biotechnol. Tech. v.5 Production of alginate beads by a rotative atomizer Begin, F.;Castaigne, F.;Goulet, J. https://doi.org/10.1007/BF00155493
  15. J. ACHE. v.40 Electrostatic droplet generation: formation Bufarski, B.;Li, Q.L.;Goosen, M.F.A.;Poncelet, D.;Neufeld, R.J.;Yunla, G. https://doi.org/10.1002/aic.690400613
  16. J. Electrostat. v.45 Monodisperse particle production: a new method to prevent drop coalescence using electrostatic forces Brandenberger, H.;Nssili, D.;Pich, V.;Widmer, F.
  17. Chem. Eng. Technol. v.10 New process (Jet cutting method) for the production of spherical beads from highly viscous polymer solutions Prsse, U.;Fox, B.;Kichhof. M.;Bruske. F.;Breford, J.;Vorlop, K.D.
  18. Process Biochem. v.35 Mass preparation and characterization of alginate microspheres Mofidi, N.;Aghai-Moghadam, M.;Sarbolouki, M.N.
  19. Eur. J. Pharm. Biopharm. v.47 Dry coating: an innovative enteric coating method using a cellulose derivative Obara, S.;Maruyama, N.;Nishiyama, Y.;Kokubo, H. https://doi.org/10.1016/S0939-6411(98)00087-3
  20. Int. Dairy J. v.13 Evaluation of encapsulation techniques of probiotics for yoghurt Wunwisa, K.;Bhesh, B.;Hilton, B. https://doi.org/10.1016/S0958-6946(02)00155-3
  21. Chem. Eng. J. v.66 Pumping characteristics of a screw agitator in a tube Rieger, F. https://doi.org/10.1016/S1385-8947(96)03150-6
  22. Chem. Eng. J. v.89 Pumping efficiency of screw agitators in a tube Rieger, F. https://doi.org/10.1016/S1385-8947(02)00022-0
  23. J. Biotechnol. v.63 A new multinozzle encapsulation/immobilisation system to produce uniform beads of alginate Brandenberger, H.;Widmer, F. https://doi.org/10.1016/S0168-1656(98)00077-7
  24. Biotechnol. Bioeng. v.70 Characterization of an encapsulation device for the production of monodisperse alginate beads for cell immobilization Serp, D.;Cantana, E.;Heinzen, C.;Stockar, U.V.;Marison, I.W. https://doi.org/10.1002/1097-0290(20001005)70:1<41::AID-BIT6>3.0.CO;2-U
  25. J. Microbiol. Biotechnol. v.9 Improvement of Bifidobacterium longum stability using cell-entrapment technique Woo, C.J.;Lee, K.Y.;Heo, T.R.
  26. J. Memb. Sci. v.160 Characteristics of sodium alginate membranes for the pervaporation dehydration of ethanol-water and isopropanol-water mixtures Huang, R.Y.M.;Pal, R.;Moon, G.Y. https://doi.org/10.1016/S0376-7388(99)00071-X