• Journal of Microbiology and Biotechnology
• /
• 제23권10호
• /
• pp.1413-1421
• /
• 2013

The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, $C20:5{\omega}-3$) and docosahexaenoic acid (DHA, $C22:6{\omega}-3$) that are important to human health. Here, we report a functional characterization of a ${\Delta}4$-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2005년도 추계 종합학술대회 논문집
• /
• pp.144-148
• /
• 2005

최근 디지털 환경의 발전으로 서사의 중요성이나 가치에 대해 큰 관심이 모아지고 있지만, 실질적으로 서사분석에 관한 구체적인 연구방법에 대해서는 아직 미흡한 단계에 있다. 특히, 애니메이션의 서사구조에 대한 연구는 주로 일본의 <아니메>와 미국 디즈니 애니메이션, 단편 애니메이션을 중심으로 이루어지고 있으며, 작품에 대해 상호비교의 관점이 아닌 각각의 작품에 대한 개별적인 서사분석이 행해지고 있다. 본 연구는 고맥락 문화의 대표국인 중국과 저맥락 문화의 대표국인 미국에서 제작된 애니메이션 가운데 동일한 테마의 작품인 <뮬란>에 대한 비교분석을 통해 문화적 맥락이 다른 두 나라의 애니메이션에 있어서의 서사적 특성을 밝히는데 그 의의가 있다.

• 한국콘텐츠학회논문지
• /
• 제7권6호
• /
• pp.119-126
• /
• 2007

최근 디지털 환경의 발전으로 서사의 중요성이나 가치에 대해 큰 관심이 모아지고 있지만, 실질적으로 서사분석에 관한 구체적인 연구방법에 대해서는 아직 미흡한 단계에 있다. 특히, 애니메이션의 서사구조에 대한 연구는 주로 일본의 <아니메>와 미국 디즈니 애니메이션, 단편 애니메이션을 중심으로 이루어지고 있으며, 작품에 대해 상호비교의 관점이 아닌 각각의 작품에 대한 개별적인 서사분석이 행해지고 있다. 애니메이션 감상수업에 있어서도 객관적이며 과학적인 분석방법에 대한 교수법 개발이 아직 체계화되지 않고 있는데, 이러한 서사분석의 방법론의 개발은 애니메이션 감상수업 교수법으로서 활용할 수 있는 하나의 방법이 될 것이라고 생각한다. 본 연구는 고맥락 문화의 대표국인 중국과 저맥락 문화의 대표국인 미국에서 제작된 애니메이션 가운데 동일한 테마의 작품인 <뮬란>에 대한 비교분석을 통해 문화적 맥락이 다른 두 나라의 애니메이션에 있어서의 서사적 특성을 밝히고 있으며, 애니메이션 감상수업을 위한 교수법 제안에 그 의의를 둔다.

• 한국방송∙미디어공학회:학술대회논문집
• /
• 한국방송공학회 1999년도 KOBA 방송기술 워크샵 KOBA Broadcasting Technology Workshop
• /
• pp.119-124
• /
• 1999

This paper proposes an evolutionary model for automatically generating artificial creatures of various shapes and colors according to insect ecology. This model offers a novel way to naturally evolve the shapes and colors of artificial creatures. The evolutionary model used in our research is based on Genetic Algorithms (GA). In this paper, artificial Computer Graphics(CG) creatures develop into various shapes and colors according to the evolutionary model. Later, they can be used as CG animated characters. This model also solves the problem of reducing the time and labor cost for mass production of various characters. It could be used in such areas as the cavalry battle scene in Disney's animation, “Mulan”. Our approach has two steps. At first, artificial creatures move according to information gathered form the five senses. This information is also used for generating the shapes of the five sense organs[1]. Then, based on the GA, evolutionary mode[2], we prepare prototype creatures, which evolve into various shapes and different colors in alternating generations. Finally, our evolutionary model successfully generates various character shapes and colors automatically.

• Journal of Microbiology and Biotechnology
• /
• 제19권10호
• /
• pp.1098-1102
• /
• 2009

Gamma-linolenic acid (GLA, C18:3 ${\Delta}^{6,9,12}$) is synthesized by a delta-6 fatty acid desaturase using linoleic acid (LA, C18:2 ${\Delta}^{9,12}$) as a substrate. To enable the production of GLA in the conventional yeast Pichia pastoris, we have isolated a cDNA encoding the delta-6 fatty acid desaturase from Cunninghamella echinulata MIAN6 and confirmed its function by heterogeneous expression in P. pastoris. Sequence analysis indicated that this cDNA sequence has an open reading frame of 1,404 bp, which encodes a 52 kDa peptide of 468 amino acids. This sequence has 64% identity to the previously reported delta-6 fatty acid desaturase from Rhizopus oryzae. The polypeptide has a cytochrome b5 domain at the N-terminus including the HPGG motif in the heme-binding region, as reported for other delta-6 fatty acid desaturases. In addition, this enzyme differs from other desaturases by the presence of three possible N-linked glycosylation sites. Analysis of the fatty acid composition demonstrated the accumulation of GLA to the level of 3.1% of the total fatty acids. Notably, the amounts of ginkgolic acid (C17:1) and palmitic acid (C16:0) were increased from 1.3% to 29.6% and from 15% to 33%, respectively. These results reveal that the modification of the fatty acid biosynthetic pathway by genetic manipulation in order to produce specific polyunsaturated fatty acids in P. pastoris is a promising technique.

• 디자인학연구
• /
• 제20권1호
• /
• pp.55-64
• /
• 2007

비언어적 의사소통 수단은 언어적 의사소통을 보조하고 대체한다는 점에서 매우 중요하다. 다양한 비언어적 의사소통 수단 중 신체언어는 몸을 통한 의사소통 전반을 말한다. 애니메이션의 경우, 시나리오 전달에 있어서 캐릭터가 가장 중요한 역할을 하므로 이와 같은 신체언어 연출은 매우 중요한 요소 중 하나이다. 애니메이션의 의사소통 구조는 일반적인 의사소통 구조와는 달리 이중적인 의사소통 구조를 가지는데, 첫째는 화자 캐릭터와 청자 캐릭터 사이의 의사소통이고, 둘째는 영상과 관객 간의 의사소통으로 이는 첫 번째 의사소통 구조까지 모두 포함한다. 본 연구에서는 이러한 이중적 구조에 기반하여 애니메이션의 의사소통 구조를 담화내적, 담화외적 구조로 나누고, 기능적 분류법을 이용하여 캐릭터의 신체언어를 적응, 상징, 설명, 조정, 감정표현 행위로 분류하였다. 특히 본 연구의 분석 애니메이션에서 큰 비중을 차지한 설명행위는 화용론적 분류인 발화 행위론으로 세분화하였다. 그리고 분류된 각각의 신체언어를 분석하여 애니메이션에서 표현되는 신체언어의 특징을 규명하고, 효과적인 시나리오 전달과 관객의 감정이입을 도울 수 있는 효율적인 신체언어를 제안하고자 하였다.

• Journal of Information Technology Applications and Management
• /
• 제27권6호
• /
• pp.1-14
• /
• 2020

Since the birth of animation, the United States has created many classic characters. For example, Mickey Mouse and Minnie, Tom and Jerry, Lion King, and Nemo, which are imaged with animal images, include Snow White, Bell, Mulan, and Aladdin. In addition, there are villain characters designed with strong personality and personality design, such as Snow White's stepmother Grimheel, Ursula, Bad Girl Crew El Radville, Scar, Captain Hook, and so on. These animation characters have been remembered for a long time with stories in people's minds, and have also brought laughter and emotion to people all over the world, which has brought a lot of business value to animation companies. Recently, the villain of American animation works is becoming more and more suited to the taste of the audience. The villain is not a symbolic image of the brutality we have seen before. They are not only visual images with rich and diverse personalities, but are also designed to suit the tastes of the public with a multifaceted inconsistency. They appear as ordinary people in our lives in works, or as powerful people who are not realistic. The villain characters designed in this way are real human miniatures appearing in the relationships in our lives, and they cannot judge good or bad only by their appearance. Through the study of villains in American animation, many villains in American animation were summed up, and villains could be classified into three types: brutal, violent, and sneaky. Based on this, it was possible to analyze the appearance and attire of the villain character in American animation, and to create a vivid and popular image of the villain, it was found that the character of the character should be emphasized when constructing the shape and costume of the villain character. In conclusion, the attractive formation of villain characters is an important part for successful animation. The production of vivid and long-lasting villain characters must begin with detailed settings such as personality, shape, and dress from the planning stage, which is not only the intention of the producer, but also a reflection of the aesthetic psychology that society should pay attention to today.

• BMB Reports
• /
• 제51권11호
• /
• pp.572-577
• /
• 2018

Recent studies showed that the PD-1/PD-L1 checkpoint blockade is a dramatic therapy for melanoma by enhancing antitumor immune activity. Currently, major strategies for the PD-1/PD-L1 blockade have mainly focused on the use of antibodies and compounds. Seeking an alternative approach, others employ endogenous proteins as blocking agents. The extracellular domain of PD-1 (ePD1) includes the binding site with PD-L1. Accordingly, we constructed a PD-1-based recombinantly tailored fusion protein (dFv-ePD1) that consists of bivalent variable fragments (dFv) of an MMP-2/9-targeted antibody and ePD1. The melanoma-binding intensity and antitumor activity were also investigated. We found the intense and selective binding capability of the protein dFv-ePD1 to human melanoma specimens was confirmed by a tissue microarray. In addition, dFv-ePD1 significantly suppressed the migration and invasion of mouse melanoma B16-F1 cells, and displayed cytotoxicity to cancer cells in vitro. Notably, dFv-ePD1 significantly inhibited the growth of mouse melanoma B16-F1 tumor cells in mice and in vivo fluorescence imaging showed that dFv-ePD was gradually accumulated into the B16-F1 tumor. Also the B16-F1 tumor fluorescence intensity at the tumor site was stronger than that of dFv. This study indicates that the recombinant protein dFv-ePD1 has an intensive melanoma-binding capability and exerts potent therapeutic efficacy against melanoma. The novel format of the PD-L1-blocked agent may play an active role in antitumor immunotherapy.