• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.716-721
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• 2005

This experiment was designed to investigate the effect of Farfarae Flos(FF) on immune cells and cytokines in murine asthma model. C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 9 weeks(3 times a week). The experimental group was treated with Farfarae Flos extract(FF) for the later 6 weeks(5 times a week). We measured IL-4, 1L-5, 1L-13, IgE, IFN-${\gamma}$ in bronchoalveolar lavage fluid of ovalbumin induced asthmatic mouse and observed murine lung tissue. The results were obtained as follows: Total leukocytes and eosinophils in BALF of the mice group treated with FF decreased significantly compared with those of control group. The concentration of IL-4, IL-5, IL-13, IgE in BALF of the mice group treated with FF decreased significantly compared with those of control group. The concentration of IFN-${\gamma}$ in BALF of the mice group treated with FF increased significantly compared with that of control group. According to the above results, it is suggested that FF extract might be useful applied for prevention and treatment of allergic asthma.

• Korean Journal of Food Science and Technology
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• v.34 no.3
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• pp.518-523
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• 2002

Administration of hot water extracts from Acanthopanax senticosus (GF-2) prophylactically and therapeutically inhibited the systemic anaphylactic shock induced by compound 48/80 in mouse. GF-2 significantly inhibited the production of histamine and eosinophyl in mouse serum through the injection of compound 48/80 in a dose-dependent manner. GF-2 inhibited dose dependently $TNF-{\alpha}$ production of peritoneal exudative cells activated by lipopolysaccharide. Intraperitoneal injection of GF-2 suppressed the production of IgG1 and IgE antibodies in mice immunized with a mixture of ovalbumin and aluminium hydroxide. These results suggest that GF-2 may be beneficial for the treatment of nonspecific and specific anaphylactic reactions and can be potentially applied to the treatment of allergic diseases.

• Archives of Pharmacal Research
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• v.28 no.12
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• pp.1350-1357
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• 2005

Nuclear factor kappa B (NF-$\kappa$B) regulates the expression of multiple cytokines, chemokines, and cell adhesion molecules that are involved in the pathogenesis of asthma. We investigated the anti-asthmatic effects and the mechanism of action of DA-9201, an extract of the black rice, in a mouse model of asthma. Mice immunized with ovalbumin (OVA) were administered with DA-9201 (30, 100 or 300 mg/kg) or dexamethasone (DEXA, 3 mg/kg) for 2 weeks and challenged with aerosolized OVA during the last 3 days. Anti-asthmatic effects were assessed by means of enhanced pauses, level of total lgE and Th2 cytokines in plasma or bronchoalveolar lavage fluid (BALF), the percentage of eosinophils in BALF, and histopathological examination. The expression of NF-$\kappa$B in nuclear and cytoplasmic fraction and its DNA-binding activity in lung tissues were analyzed by means of Western blotting and electrophoretic gel mobility shift assay (EMSA), respectively. DA-9201 significantly reduced airway hyperrespon-siveness (AHR), total lgE level in plasma and BALF, IL-4, IL-5, and IL-13 levels in BALF, and the percentage of eosinophils in BALF. Tissue inflammation was significantly improved by DA­9201 treatment. In addition, DA-9201 dramatically suppressed the expression of NF-$\kappa$B and its DNA-binding activity. These results suggest that DA-9201 may be useful for the treatment of asthma and its efficacy is related to suppression of NF-$\kappa$B pathway.

• IMMUNE NETWORK
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• v.14 no.5
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• pp.249-254
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• 2014

Allergic asthma is a chronic pulmonary inflammatory disease characterized by reversible airway obstruction, hyperresponsiveness and eosinophils infiltration. Toll-like receptors (TLRs) signaling are closely associated with asthma and have emerged as a novel therapeutic target in allergic disease. The functions of TLR3 and TLR4 in allergic airway inflammation have been studied; however, the precise role of TIR-domain-containing adapter-inducing interferon-${\beta}$ (TRIF), the adaptor molecule for both TLR3 and TLR4, is not yet fully understood. To investigate this, we developed a mouse model of OVA-induced allergic airway inflammation and compared the severity of allergic airway inflammation in WT and $TRIF^-/^-$ mice. Histopathological assessment revealed that the severity of inflammation in airway inflammation in TRIF-deficient mice was comparable to that in WT mice. The total number of cells recovered from bronchoalveolar lavage fluid did not differ between WT and TRIF-deficient mice. Moreover, TRIF deficiency did not affect Th1 and Th2 cytokine production in lung tissue nor the level of serum OVA-specific IgE, $IgG_1$ and $IgG_{2c}$. These findings suggest that TRIF-mediated signaling may not be critical for the development of allergic airway inflammation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.3
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• pp.710-718
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• 2006

The present study was carried out to investigate the effect of Chihyo-san (CHS) administration on asthma induced by Alum/OVA treatment in the mice. In CHS-treated animal group, lung weight, which was increased after asthma induction, was significantly decreased, and total number of cells in the lung, peripheral lymph node (PLN) and spleen tissue was significantly decreased in CHS-treated group compared to the asthma control group. The number of immune cells including natural killer (NK) cells in asthmatic animals was largely regulated by CHS treatment, showing a similar pattern as that of CsA-treated positive control group. Levels of mRNAs encoding inflammatory cytokines IL-5, IL-13, $TNF-{\alpha}$, and eotaxin were determined by RT-PCR in the lung tissue and showed decreases in CHS-treated group to the similar levels of CsA-treated control group, Histamine level in the serum was significantly lower in CHS-treated group than asthma-induced control group. Both haematoxylin and eosin staining and Masson's trichrome staining results showed decreased number of inflammatory cells, reduced immune cell infiltration, and normalized epithelial cell layering in the bronchial tissue of CHS-treated mouse group. Thus, the present findings suggest that CHS may be useful for protecting bronchial tissues from consistent inflammatory damages that occur in asthma patients.

• The Journal of Internal Korean Medicine
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• v.26 no.1
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• pp.156-168
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• 2005

Objective : The purpose of this research is to examine the effects of Pinelliae Rhizoma (PR) extract on immune cells and cytokines in ovalbumin (OVA)-induced asthmatic mice. Methods : In vivo, C57BL/6 mice were sensitized and handicapped by OVA for 12 weeks. During this experiment, the one group was then treated with PR extract for the 8 weeks (3 times per week) and analyzed by ELISA and flow cytometer. Results : In vivo, there were significant decreases in eosinophils, IL-4, IL-5, IL-13, IgE in BALF (bronchoalveolar lavage fluid) compared with that of control group. However, $IFN-{\gamma}$ in BALF of GS group increased significantly, compared with that of control group. Additionally, the population of $CD3e^-/CCR3^+,\;CD69^+/CD3e^+,\;IgE^+/B220^+,\;CD11b^+/Gr-l^+$ cells in the PR group decreased, compared with that of control group. Conclusion : The results of this study support a role for PR as an effective treatment for asthma in its experimental success in significantly decreasing inflammation and asthma reactions, and in increasing $IFN-{\gamma}$, which helps prevent such reactions.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.237-244
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• 2015

In this study, essential oils were extracted from the leaf of Chamaecyparis obtusa (CLEO), indigenous to Korea, CLEO constituents were analysed, and the effects of CLEO on airway hyperresponsiveness (AHR) and airway inflammation (AI) were investigated in Ovalbumin (OVA)-induced asthma mouse model. Terpenoid components among identified CLEO constituents made up more than 80%. The CLEO-treated group in comparison to the control group showed reduced AHR, the decrease of eosinophil number in the bronchoalveolar lavage fluid (BALF), reduced specific anti-OVA IgE level in the serum, and a significant reduction in Th2 cytokines levels in the BALF with concentration. We concluded that CLEO have an alleviating effect on asthma-like symptoms such as AHR and AI. Further studies about antiasthmatic effect are necessary on the focus of single component of CLEO.

• Korean Journal of Medicinal Crop Science
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• v.20 no.2
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• pp.129-135
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• 2012

The feature of asthma are airway inflammation (AI), reversible airway obstruction, and an increased sensitivity to bronchoconstricting agents, elevated airway hyperresponsiveness (AHR), excess production of Th2 cytokines, and eosinophil accumulation in the lungs. This study was performed to investigate if oral administration of $Scutellaria$ $baicalensis$ Georgi water extracts (SBG) have the antiasthmatic potential for the treatment of asthma. Asthmatic HI and AHR were induced by systemic sensitization to ovalbumin (OVA) with intratracheal instillation with 0.1 mg/mL of diesel exhaust particles (DEP) suspension once a week for 10 weeks in BALB/c mice. SBG was orally administered with the concentraion of 200 mg/kg 5 days a week for 10 weeks. Long-term SBG treatment suppressed the eosinophil infiltration into airways from blood, the asthmatic AI and AHR by attenuating the production of cytokine IL-4, IL-5 and IL-13, histamine and OVA-specific IgE. Our data suggest that SBG has inhibitory effects on AI and AHR in a mouse model of asthma, may act as a potential Th2 cytokine antagonist, and may have a therapeutic effect on allergic asthma.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.2
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• pp.446-451
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• 2005

This experiment was designed to investigate the effect of Mominis Placenta herbal acupuncture solution(HP-HAS) on immune cells and cytokines in murine asthma model. In vivo C57BL/6 mice were sensitized and challenged with OVA for 12 weeks. The experimental group was treated with Hominis Placenta herbal acupuncture solution(HP-HAS) at P'yesu(BL13) for the later 8 weeks(3 times a week) and analyzed by ELISA, flow cytometer. The results were obtained as follows Eosinophils in BALF(bronchoalveolar lavage fluid) of HP-HAS group decreased significantly compared with that of control group. IL-4, IL-5, IL-13, IgE in BALF of HP-HAS group decreased significantly compared with that of control group. Number of $CD3e^-/CCR3^+$, $CD69^+/CD3e^+$, $CD11b^+/Gr-1^+$ cells in the HP-HAS group decreased compared with that of control group.

• Journal of the Korean Electrochemical Society
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• v.25 no.1
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• pp.13-21
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• 2022

There is an increasing interest in monitoring of specific biomarker for determining progression of a disease or efficacy of a treatment. Conventional method for quantification of specific biomarkers as enzyme linked immunosorbent assay (ELISA) has high material costs, long incubation periods, requires large volume of samples and involves special instruments, which necessitates clinical samples to be sent to a lab. This paper reports on the development of an electrochemical biosensor to measure total immunoglobulin E (IgE), a marker of asthma disease that varies with age, gender, and disease in concentrations from 0.3-1000 ng/mL with consuming 20 µL volume of whole blood sample. The sensor provides rapid, accurate, easy, point-of-care measurement of IgE, also, sequential monitoring of total IgE with ovalbumin (OVA) induced mice is another application of sensor. Taken together, these results provide an alternative way for detection of biomarkers in whole blood with low volumes and long-term ex-vivo assessments for understanding the progression of a disease.