• The Plant Pathology Journal
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• v.40 no.1
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• pp.16-29
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• 2024

The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.

• ALGAE
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• v.39 no.2
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• pp.75-96
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• 2024

Rhodomonas (Cryptophyceae) and species assigned to this genus have undergone numerous taxonomic revisions. This also applies to R. marina studied here as it was originally assigned as a species of Cryptomonas and later considered a variation of R. baltica, the type species. Despite being described more than 130 years ago, R. marina still lacks a comprehensive characterization. Light and electron microscopy were employed to delineate a strain from western Greenland. The living cells were 18 ㎛ long and 9 ㎛ wide, elliptical in shape with a pointed to rounded posterior and truncated anterior in lateral view. Two sub-equal flagella emerged from a vestibulum, where also a furrow extended. In transmission electron microscopy, the furrow was associated with a tubular gullet and the pyrenoid embedded in a deeply lobed chloroplast. The chloroplast contained DNA in perforations and was surrounded by starch grains. A tubular nucleomorph was enclosed within the pyrenoid matrix. In scanning electron microscopy, the inner periplast consisted of rectangular plates with rounded edges and posteriorly these were replaced by a sheet-like structure. The water-soluble pigment was Crypto-Phycoerythrin type I (Cr-PE 545). A phylogenetic inference based on SSU rDNA confirmed the identity of strain S18 as a species of Rhodomonas as it clustered with congeners but also Rhinomonas, Storeatula, and Pyrenomonas. These genera formed a monophyletic clade separated from a diverse assemblage of other cryptophyte genera. To further explore the phylogeny of R. marina a concatenated phylogenetic analysis based on the SSU rDNA-ITS1-5.8S rDNA-ITS2-LSU rDNA region was performed but included only closely related species. The secondary structure of nuclear internal transcribed spacer 2 was predicted and compared to similar structures in related species. Using morphological and molecular signatures as diagnostic features the description of R. marina was emended.

• Journal of Veterinary Science
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• v.25 no.2
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• pp.32.1-32.14
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• 2024

Background: Aortic arch (AA) branching patterns vary among different mammalian species. Most previous studies have focused on dogs, whereas those on raccoon dogs remain unexplored. Objectives: The objective of this study was to describe the AA branching pattern in raccoon dogs and compare their morphological features with those of other carnivores. Methods: We prepared silicone cast specimens from a total of 36 raccoon dog carcasses via retrograde injection through the abdominal aorta. The brachiocephalic trunk (BCT) branching patterns were classified based on the relationship between the left and right common carotid arteries. The subclavian artery (SB) branching pattern was examined based on the order of the four major branches: the vertebral artery (VT), costocervical trunk (CCT), superficial cervical artery (SC), and internal thoracic artery (IT). Results: In most cases (88.6%), the BCT branched off from the left common carotid artery and terminated in the right common carotid and right subclavian arteries. In the remaining cases (11.4%), the BCT formed a bicarotid trunk. The SB exhibited various branching patterns, with 26 observed types. Based on the branching order of the four major branches, we identified the main branching pattern, in which the VT branched first (98.6%), the CCT branched second (81.9%), the SC branched third (62.5%), and the IT branched fourth (52.8%). Conclusions: The AA branching pattern in raccoon dogs exhibited various branching patterns with both similarities and differences compared to other carnivores.

• Economic and Environmental Geology
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• v.47 no.4
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• pp.431-439
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• 2014

The purposes of this research were to investigate the enrichment of metal-reducing bacteria from KURT groundwater and the identification of the microbial diversity by 16S rRNA as well as to examine microbial Fe(III)/Mn(IV) reduction and to analyze morphological features of interactions between microbes and precipitates and their mineralogical composition. To cultivate metal-reducing bacteria from groundwater sampled at the KURT in S. Korea, different electron donors such as glucose, acetate, lactate, formate, pyruvate and Fe(III)-citrate as an electron accepter were added into growth media. The enriched culture was identified by 16S rRNA gene sequence analysis for the diversity of microbial species. The effect of electron donors (i.e., glucose, acetate, lactate, formate, pyruvate) and electron acceptors (i.e., akaganeite, manganese oxide) on microbial iron/manganese reduction and biomineralization were examined using the 1st enriched culture, respectively. SEM, EDX, and XRD analyses were used to determine morphological features, chemical composition of microbes and mineralogical characteristics of the iron and manganese minerals. Based on 16S rRNA gene analysis, the four species, Fusibacter, Desulfuromonas, Actinobacteria, Pseudomonas sp., from KURT groundwater were identified as anaerobic metal reducers and these microbes precipitated metals outside of cells in common. XRD and EDX analyses showed that Fe(III)-containing mineral, akaganeite (${\beta}$-FeOOH), reduced into Fe(II)/Fe(III)-containing magnetite ($Fe_3O_4$) and Mn(IV)-containing manganese oxide (${\lambda}-MnO_2$) into Mn(II)-containing rhodochrosite ($MnCO_3$) by the microbes. These results implicate that microbial metabolism and respiratory activities under anaerobic condition result in reduction and biomineralization of iron and manganese minerals. Therefore, the microbes cultivated from groundwater in KURT might play a major role to reduce various metals from highly toxic, mobile to less toxic, immobile.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.2
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• pp.177-188
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• 1985

To clarify the characteristics and genesis of clayey terrace soils in Yeongnam area, macro-morphological features and soil profile development indexes were investigated with the sequential soils in Yeongcheon (inland region) and in Yeongil (coastal region). The results are summarized as follows; 1. According to the physiogaphy and landuse, the terrace soils were discernible to well drained yellowish red (5 YR) profile of higher terrace (Bancheon series), moderately well drained reddish yellow (7.5 YR) profile of middle terraces (Upyeong and Hwadong series), and lower terraces (Deogpyeong and Geugrag series) which were moderately well to imperfectly drained by paddy-fication. 2. The roundness and sphericity of the gravels contained in the terrace deposits were ranged around 0.543-0.546 and 0.723-0.722, respectively. The rounded to well rounded gravels were resemble to typical alluvial origins. 3. The amount of clay minerals formed in the soil horizons per 100g of parent materials were 50.8-30.7g while the rates of the clay leached were 30.1-7.4%, and the higher terrace had the more leaching rates. 4. The index of profile development of the terrace soils ranged from 37.95 to 22.01 and the index were positively correlated with relative elevations of the soils. The rates of clay leaching were positively correlated with the ratios of clay in the illuvial horizons to elluvial horizons. 5. A similarity was observed among cumulative grain size curves of the terrace soils, but the patterns of recalculated silt free cumulative grain size curve of terrace deposits in Upyeong soils in Yeongil area were abruptly different from down layer that suggest the soil to have bisequum profile.

• Development and Reproduction
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• v.13 no.4
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• pp.281-289
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• 2009

The slime flounder Microstomus achne were caught at Geomun Island, Yosu-si, Jeollanamdo from January to March in 2006. The fertilized eggs were observed for morphological development of egg, embryo and larva. Eggs were colorless transparent, separative pelagic, absent of oil globule, and the diameter was 1.64${\pm}$0.03 mm (n=50). The eggs were hatched at 168 hours 40 minutes after fertilization in the range of $9.8\sim13.0^{\circ}C$ (mean $11.4{\pm}1.6^{\circ}C$). Total length of newly hatched larva was 4.05${\pm}$0.18 mm (n=20). The larva had developed membranous fin showing waterdrop-shaped structure, and their mouth and anus were not open. The myotomes were 14~15+33~34=47~49. The egg yolks were 1.64${\pm}$0.12 mm in major axis, and 1.23${\pm}$0.19 mm in minor axis. At 12 days after hatching, the total length was 7.32${\pm}$0.42 mm(n=20). The egg yolk was completely absorbed and transferred to post larval stage. Star-shaped melanophores and branch-shaped xanthophores in the edge of membranous fin were more densed. Chrysanthemum-shaped melanophores in the notochord were densed and formed 4~5 melanophore bands. At 90~93 days after hatching, morphological features of the larva, 19.91${\pm}$1.63 mm TL(n=20), were transferred to juvenile stage showing similar features with those of the adult fish.

• Applied Microscopy
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• v.18 no.1
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• pp.1-20
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• 1988

There's been arguments on the different morphological status between the nucleus accumbens septi and nucleus fundus striati of ventral striatum. Authors carried out the comparative study on the neuronal cell types of these nuclei, in the chick and the rat. Results are summarized as follows: In the nucleus accumbens septi of the chick, there found 3 main cell types. Type I cells are oval or spindle-shaped. They are the most abundant cell types, comprising more than 80% of neurons. The pale nucleus is usually indented. The cytoplasm is also pale and contains small amount of mitochondria, rough r-ER and Golgi complexes. This cell has a few symmetric synapses on the cell membrane. Type II cells are pale large cells. They are polygonal or irregularly-shaped. They contain pale spherical nucleus, and the pale cytoplasm with relatively large amount of mitochondria, free ribosomes and well-developed Golgi complexes. Some axo-somatic synapes are found on the cell. Type III cells are oval or spherical-shaped. The nucleus is relatively pale and large, In the dense cytoplasm, well developed. r-ER formed typical Nissl's body, and there found many mitochondria, ribosomes and lysosomes. In the chick fundus striati nucleus, there also found 3 main cell types. Type I cells are small and spindle-shaped. This type is the most abundant one and constitutes more the 80% of the neurons. Morphological features other than it's shape, is generally similar with that of Type I cell in the nucleus accumbens. Type II cells are irregularly shaped large cells. Dense cytoplasm contains large amount of cell organelles. Some axo-somatic synapses are found. Type III cells are small dense cells. This oval cell contains the oval nucleus, and the plentiful cytoplasm with well developed r-ER, ribosomes and mitochondria. In the nucleus accumbens septi of the rat, there found 4 main cell types. Type I cells are small, oval or spherical cells, comprising more than 90% of all the neurons. Spherical nucleus shows typical chromatin rim along the nuclear membrane. Dense cytoplasm contains many ribosomes and mitochondria. Type II cells are large oval cells. The eccentric nucleus is deeply invaginated. Pale cytoplasm contains large amount of ribosomes, Golgi complexes, mitochondria, and dense bodies. Type III cells are pale, large, oval cells. They contain moderate amount of ribosomes and mitochondria, and some scattered stacks of r-ER. Type IV cells are small pale cells. Small oval nucleus is indented and shows chromatin rim. Only small amount of ribosomes and mitochondria can be found. In the nucleus fundus striati of the rat, there also found 4 main cell types. Type I cells are spherical or oval cells, comprising more than 90% of the neurons. The chromatin rim of the spherical nucleus is not so prominent as compared to the rim of type I cell in the nucleus accumbens septi. The cytoplasm contains moderate amount of mitochondria, ribosomes and some scattered r-ER. A few axo-somatic synapses were found. Type II cells are small round or polygonal cells. Golgi complexes are especially well-developed in this cell type. The cytoplasm also contains moderate amount of mitochondria, ribosomes, and dense bodies. Type III cells are small cells. The large nucleus shows prominent chromatin rim. The cytoplasm contains many ribosomes and mitochondria. Type IV cells are large, spheircal or oval cells. The nucleus is deeply indented. The plentiful cytoplasm contains large amount of ribosomes, mitochondria, Golgi complexes, neurotubules, but not r-ER. In the present study, it is clear that the nucleus accumbens septi and the nucleus fundus striati are independant cell groups, according to their cytoarchitectonics and the ultrastructural features of their cell types.

• Korean Journal of Veterinary Research
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• v.21 no.2
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• pp.105-112
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• 1981

The morphological and structural studios of Anisakinae larva has been carried out since Sept. of 1980. The larva were collected from naturally infested eleven swine of 1,531 examined at Kwang-Ju abattoir and from marine fishes, Somber japonicus, bought at Kwang-Ju fish market. The results observed were as follow : 1. Anisakis larva found in the stomach wall and on the surface of the mucosa were more or less degenerated. According to the progress of degeneration, the cross sections showed varied structures (Fig. 6, 7). 2. Size of the larva both from swine and fishes were measured respectively in average(mm); 18.0 and 18.7 in body length, 0.30 and 0.41 in body width, 1.64 and 1.68 in esophagus(muscular-part), 0.56 and 0.67 in ventriculus (glandular part), and 0.13 and 0.12 in tail. It was notable that body length of the larva in this present data, 18.0mm and 1.87mm, were shorter than those in previous dada, 24.3mm from human cases and 28.4mm from, however, the present data were almost similar to the data, 1.75mm, from swine case. 3. The Boring tooth, Mucron, long ventriculus and short round tail were observed in the larva of this present study. These structures were differentiated from Anisakis type II larvae which was provided with short ventriculus, and conical and tapering tall without mucron. 4. The ventricular appendix and intestnal caecum were not present in the larva. These might be differentiated from other Anisakidae larva such as Terranova larvae, Contracaecum larvae, Raphidascaris larvae and Thynnascaris larvae. 5. The findings through the histological observation were a pair of Y-shaped or butterfly-shaped lateal chords, ventral and dorsal chords, excretory(Renette) cell, high columnar epithelial cells of digestive tract and muscle cells. These morphological characteristics revealed varied features in the structures in the degenerative degree of the larva in the stomach wall. 6. The above-mentioned characteristics of the larva observed could be indentified as Anisakis type I larvae. 7. The reports on natural infestation of domestic animal with Anisakis type I larvae were two swine cases in Korea and Japan respectively, On the other hand two human cases of the larva were reported in Korea and more than one thousand cases in Japan. In Twiwan no reports of human and domestic animal cases could be found.

• Korean Journal of Remote Sensing
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• v.33 no.5_3
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• pp.835-854
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• 2017

Government regulations and policies are important means of restraining the indiscreet expansion of urban areas. According to the standards from those means, it is clear that the fluctuation of forest green proportion encroached by the increase of urban space is obvious. In this study, we interpreted the changes of urban areas as well as the green ones owing to the urban expansion by the decades from 1996, with focusing on the cities of Seoul and Daegu highly developed in South Korea. The purpose of this study is to analyze the spatial expansion and morphological characteristics of urban land cover using not only satellite imageries (1996, 2006, 2016). but also the urban expansion intensity index (UEII) and GUIDOS program. Ultimately, this study is to compare the changes in the rate of forests due to urban expansions annually analyzed based on areas of forest elevation, slope, and the area of single forest patch. In Seoul, the expansion begun from urban space to suburban areas was comparatively rapid, which led the forest fragmentation and the gradual decline of the single patch. However, when it comes to DEM (Digital elevation model) and slope above a certain standard, by the development regulations, there was little decrease in area by anthropogenic developments. The city of Daegu has increased at a slow speed since 1996 in urban and suburban areas, whereas green forests have greatly increased through green forest conservation campaigns. In this way, as to the government policies and regulations, the quantitative and morphological expansion of cities owing to development could be controlled and forest spaces could be preserved as well. Therefore, regulations and policies by the government should be appropriately utilized for sustainable cities.

• Journal of Korean Society of Forest Science
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• v.113 no.1
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• pp.88-96
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• 2024

This study was conducted to determine the optimal light conditions for the in situ and ex situ conservation and restoration of Osmanthus insularis, a rare plant species in South Korea. Evaluations included the growth performance, leaf morphological features, photosynthetic characteristics, and photosynthetic pigment contents of seedlings grown from April to November under different light conditions (100%, 55%, 20%, and 10% relative light intensity). The shoot lengths and root collar diameters did not differ significantly with relative light intensity. The dry weights of leaves, stems, and roots and the leaf number were highest at 55% relative light intensity. The leaf shape showed morphological acclimation to light intensity, with leaf area decreasing and thickness increasing as the relative light intensity increased. Several leaf parameters, including photosynthetic rate and stomatal conductance at light saturation point, net apparent quantum yield, and dark respiration, as well as chlorophyll a, chlorophyll b, and carotenoid contents, were all highest at 55% relative light intensity. Under full light conditions, the leaves were the smallest and thickest, but the chlorophyll content was lower than at 55% relative light intensity, resulting in lower photosynthetic ability. Plants grown at 10% and 20% relative light intensity showed lower chlorophyll a, chlorophyll b, and carotenoid contents, as well as decreased photosynthetic and dark respiration rates. In conclusion, O. insularis seedlings exhibited morphological adaptations in response to light intensity; however, no physiological responses indicating enhanced photosynthetic efficiency in shade were evident. The most favorable light condition for vigorous photosynthesis and maximum biomass production in O. insularis seedlings appeared to be 55% relative light intensity. Therefore, shading to approximately 55% of full light is suggested for the growth of O. insularis seedlings.