• Title/Summary/Keyword: Mono-mono cross

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Nuclear DNA inheritance of intraspecific somatic hybrids by mono-mono cross in Pleurotus ostreatus based on URP-PCR analysis (URP-PCR 분석에 의한 느타리 단핵 계통간 교잡주의 핵 DNA 유전)

  • Kim, Eun Jung;Shin, Pyung Gyun;Jang, Kab Yeul;Kong, Won Sik;Han, Young Sook;Yoo, Young Bok
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.171-180
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    • 2014
  • The primary objective of the present study is the characterization of the hybrids of monokaryon- monokaryon (mono-mono) crosses in mushroom breeding. We employed this technique for developing superior strains from Pleurotus species strains with 85 mono-mono intraspecific hybrids of 7 combinations between six Pleurotus ostreatus strains and one Pleurotus florida strain. In this study, the results of analysis on hybridization rate, nuclear DNA patterns, and colors and yields of fruit-bodies, are presented as follows. The crossability between mono-mono crossing ranges between 50 and 93.75%. The results of the analysis on the nuclear DNA patterns of 85 hybrid strains of mono-mono crosses share the nuclei of both parents, but their genetic similarities were predominated by either parent. The hybrid strain between P. florida and P. ostreatus showed patterns more similar to P. florida, while the hybrid strain between P. ostreatus and P. ostreatus either had patterns predominated by either parent strain. The fruiting body colors of the mono-mono crosses mostly had combined colors of both parents but showed the tendency of being more similar to that of either parent. 82% of the hybrid strain indicated similar fruiting body yields compared to both parent strains, while 0% was higher and 18% were lower than both parents. The present study was able to find out and suggest superior hybrid trains by identifying the nuclear DNA patterns of hybrids between Pleurotus species as well as the characteristics of their fruiting bodies. This study expects that the advantages of the mono-mono crossing are needs to be fully utilized in mushroom breeding and it is better to develop superior strains of Pleurotus species strains together with the mono-mono crossing.

Radar Cross Section Reduction by Planar Array of Dielectric Barrier Discharge Plasma under Atmospheric Pressure (평면 배열 유전체 장벽 방전 플라즈마 발생기의 대기압에서의 레이다 단면적 감소 효과)

  • Kim, Yuna;Kim, Sangin;Kim, Doo-Soo;Lee, Yongshik;Yook, Jong-Gwan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.28 no.8
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    • pp.646-652
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    • 2017
  • The effect of plasma on mono-static radar cross section under atmospheric pressure is demonstrated when the dielectric barrier discharge actuator has plasma layer. The volume of plasma layer is increased by using planar array of electrodes. Because the incident wave has electric field which is perpendicular to the electrode array, the undesired effect on radar cross section caused by structure of plasma actuator is minimized. In experiments, mono-static radar cross section is measured at the frequencies from 2 GHz to 25 GHz. The generated plasma reduces the radar cross section at frequencies above 18 GHz, and the amount of reduction reaches to 8 dB in maximum. The reduction can be controlled by changing the peak-to-peak voltage from high voltage generator. The result shows the possibility of plasma as a flexible radar cross section controller.

Comparison of Effects of Ultraviolet and $^{60}$ Co Gamma Ray Irradiation on Nylon 6 Mono-filaments

  • Ohtsuka, Mika;Suzuki, Yoshino;Sakai, Tetsuya;Netravali, Anil N.
    • Fibers and Polymers
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    • v.5 no.3
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    • pp.225-229
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    • 2004
  • The effect of UV and $^60{Co}$ gamma radiations on the physical and mechanical properties of nylon 6 mono-filaments with different draw ratios has been studied. Specimens were exposed to either up to 25 Mrad of gamma or up to 168 hrs of intense UV irradiation. The results show that nylon mono-filaments exposed to gamma rays, with much higher quantum energy than UV, undergo a larger extent of molecular chain scission. Higher irradiation dose also results in the production of insoluble, macroscopic three-dimensional cross-linked network structure. The amorphous regions with a lower density of cohesive energy (lower molecular orientation) show a higher extent of cross linking reaction whereas amorphous regions with a higher density of cohesive energy (higher orientation) show higher extent of chain scission reaction, irrespective of UV ray or gamma ray irradiation.

A Study on the Moment Gradient factor of Mono-symmetric I Beam (일축 대칭 I 형 보의 모멘트 구배계수에 대한 연구)

  • 김윤종;임남형;박남회;강영종
    • Proceedings of the KSR Conference
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    • 2000.05a
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    • pp.439-446
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    • 2000
  • In this study, 7 dof (Including warping) beam element was developed to estimate the effects of wagner effects and load height effects on the lateral buckling strength of mono-symmetric I beam. Finite element buckling analysis of mono-symmetric I-shaped girders subjected to transverse loading applied at different heights on the cross-section were conducted. Linear moment gradient were considered, too. In these cases, girders are subjected to both single-curvature and Reverse-curvature bending. An applicability of current LRFD C$\sub$b/ on the mono-symmetric I beam was studied from the finite element results. The problems of current LRFD C$\sub$b/ occurring from load height effects and reverse curvature bending in unbraced length when applied on the mono-symmetric I beam were studied. Solutions to these problems are also presented.

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Changes of Sugar Content during Germination and Germinability in Corn (옥수수 발아시 종자내 당함량의 변화와 발아 특성)

  • 김종진;이영찬
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.768-774
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    • 1995
  • These experiments were conducted to determine the change of sugar content during germination in corn, which were analyzed by using HPLC. Germination percentage and growth rate of Golden cross bantam 70 were higher than those of Suweon 19. The emergences of radicle and plumule of Golden cross bantam 70 were faster compared to those of Suweon 19. Three major components, sucrose, glucose and fructose, were detected during germination. Content of sucrose in two tested hybrids decreased rapidly as time passes. In embryo of Suweon 19, the content of sucrose was 38.92% on 12 hours after incubation but decreased to 4.52% on 72 hours. In that of Golden cross bantam 70, it decreased rapidly more than Suweon 19 from 53.03% on 12 hours to 8.18% on 72 hours. On the other hand, the contents of glucose and fructose increased.

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Development of New Soft Contact Lens Materials Using Ester-Monomers of Itaconic Acid from Aspergillus itaconicus (Aspergillus itaconicus 유래 itaconic acid의 ester-monomer를 이용한 새로운 soft contact lens 소재 개발)

  • You, Young-Hyun;Nam, Joo-Hyeung;Kim, Bieong-Kil;Kim, Soon-Bok;Moon, Ik-Jae;Kim, Jong-Pil;Seu, Young-Bae
    • Journal of Life Science
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    • v.19 no.4
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    • pp.538-542
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    • 2009
  • In this study, we confirmed water content and oxygen permeability of new polymeric materials synthesized from itaconic acid used for soft contact lenses. In this study, we polymerized materials for soft contact lenses using HEMA (2-hydroxyethyl methacrylate), the based-monomer of soft contact lenses, EGDMA (ethylene glycol dimethacrylate) as a cross linkage agent, and the new additives mono-ester or di-ester derived from itaconic acid commercially produced by the fermentation of A. itaconicus. New polymer materials for contact lenses were synthesized with the mixture of HEMA and mono- or di-ester at different ratios and water content and oxygen permeability (Dk) was analyzed. In polymerizing HEMA and mono-ester (15%), the water content and oxygen permeability of contact lenses were found to be of good value at 57.7% and 28.6 Dk respectively. The mixture of HEMA and mono-ester is more excellent than HEMA/di-ester in regards to water content and oxygen permeability. The water content and oxygen permeability of soft contact lenses made by new polymeric materials were highly represented.

Drug Interaction of Cimetidine and Cyclosporine in Human (정상지원자에서 Cimetidine과 Cyclosporine의 약물상호작용)

  • Choi, In;Choi, Jun Shik
    • Korean Journal of Clinical Pharmacy
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    • v.7 no.2
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    • pp.51-63
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    • 1997
  • The effect of cimetidine administration on the pharmacokinetic parameters of cyclosporine were determined in healthy voluteers. This study was performed in 10 volunteers of age ranged 22-48 years and body weight 48-62 kg. This study was performed with cross-over design. Mono cyclosporine and cyclosporine metabolites was extracted from whole blood analysed by fluororescence polarization immune assay (TDX-FLX, Abbott). After coadministration of cimetidine (300 mg) with cyclosporine (300 mg) orally, maximum concentration of mono cyclosporine was significantly increased $1221{\pm}143\;ng/ml\;to\;1562{\pm}184\;ng/ml$ (P<0.05), area under the time curve of cyclosporine (12 hr) also was significantly increased $7478{\pm}829\;ng/ml{\cdot}hr\;to\;9721{\pm}879\;ng/ml{\cdot}hr$ (P<0.05) and absolute baioavailability of cyclosporine was increased $50\pm5.6\%\;to\;57.6\pm6.1\%\;(P<0.05)$ compared to control group. The blood concentrations of cyclopsorine metabolites were significantly decrased (P<0.05) after coadministration of cimetidine. In cimetidine pretreated group, blood mono cyclosporine concentrations were increased significan시y $1220.0\pm203.00\;ng/ml\;to\;1510.0\pm204.00\;ng/ml$ compared with control group (P<0.05). In the mono cyclosporine pharmacokinetic parameter after oral administration absorption rate and maximum concentration were significantly higher in cimetidine coadministered and pretreated group than control group (P<0.05). The ratio of metabolites and mono cyclosporine concentrations was decreased significantly from $70.8\%\;in\;control\;to\;34.8\%$ in coadministration of cimetidine orally. As matter of facts these reults are considered to inhibition of cyclosporine hepatic metabolism and increasing of cyclosporine absorption rate in gastrointestinal tract because of maintaining cyclosporine stability in elevated gastric pH by cimetidine. We considered, it appeares that cimetidine increase bioavailability of cyclosporine by increasing oral absorption and by decreasing hepatic clearance. But the absorption and clearance of cyclosporine was highly variable individually, and therefore we consider that cyclosporine blood level monitoring would be essential in patients with cimetidine co-administration.

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Critical buckling moment of functionally graded tapered mono-symmetric I-beam

  • Rezaiee-Pajand, Mohammad;Masoodi, Amir R.;Alepaighambar, Ali
    • Steel and Composite Structures
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    • v.39 no.5
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    • pp.599-614
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    • 2021
  • This study deals with the Lateral-Torsional Buckling (LTB) of a mono-symmetric tapered I-beam, in which the cross-section is varying longitudinally. To obtain the buckling moment, two concentrated bending moments should be applied at the two ends of the structure. This structure is made of Functionally Graded Material (FGM). The Young's and shear modules change linearly along the longitudinal direction of the beam. It is considered that this tapered beam is laterally restrained continuously, by using torsional springs. Furthermore, two rotational bending springs are employed at the two structural ends. To achieve the buckling moment, Ritz solution method is utilized. The response of critical buckling moment of the beam is obtained by minimizing the total potential energy relation. The lateral and torsional displacement fields of the beam are interpolated by harmonic functions. These functions satisfy the boundary conditions. Two different support conditions are considered in this study. The obtained formulation is validated by solving benchmark problems. Moreover, some numerical studies are implemented to show the accuracy, efficiency and high performance of the proposed formulation.

The Development of Mono-sized Micro Silicon Particles for Spherical Solar Cells by Pulsated Orifice Ejection Method

  • Dong, Wei;Masuda, Satoshi;Takagi, Kenta;Kawasaki, Akira
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09a
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    • pp.426-427
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    • 2006
  • Mono-sized silicon particles were effectively fabricated by a novel way named pulsated orifice ejection method (POEM). The particles are with very narrow particles size distribution and very small standard deviation of mean particle size. There are two different types spherical silicon particles were found. One consists of many grains mainly in random boundaries. The other consists of two or three grains with only twin orientation relationships, even single crystal in cross-section was also found within this type of spherical silicon particles.

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Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60

  • Choi, Jeom-Il;Lee, Sang-Yull;Kim, Koan-Hoi;Choi, Bong-Kyu;Kim, Myung-Jin
    • Journal of Periodontal and Implant Science
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    • v.41 no.2
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    • pp.54-59
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    • 2011
  • Purpose: The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis. Methods: In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog. Results: Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK). Conclusions: Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immuno-pathogenic mechanism of bacterial antigen-triggered autoimmune diseases.