Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60

  • Choi, Jeom-Il (Department of Periodontolog, Pusan National University School of Dentistry) ;
  • Lee, Sang-Yull (Department of Biochemistry, Pusan National University School of Medicine) ;
  • Kim, Koan-Hoi (Department of Pharmacology, Pusan National University School of Medicine) ;
  • Choi, Bong-Kyu (Department of Microbiology and Immunology, Seoul National University School of Dentistry) ;
  • Kim, Myung-Jin (Department of Periodontolog, Pusan National University School of Dentistry)
  • Received : 2010.11.15
  • Accepted : 2011.02.03
  • Published : 2011.04.30
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Abstract

Purpose: The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis. Methods: In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog. Results: Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK). Conclusions: Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immuno-pathogenic mechanism of bacterial antigen-triggered autoimmune diseases.

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References

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