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http://dx.doi.org/10.5051/jpis.2011.41.2.54

Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60  

Choi, Jeom-Il (Department of Periodontolog, Pusan National University School of Dentistry)
Lee, Sang-Yull (Department of Biochemistry, Pusan National University School of Medicine)
Kim, Koan-Hoi (Department of Pharmacology, Pusan National University School of Medicine)
Choi, Bong-Kyu (Department of Microbiology and Immunology, Seoul National University School of Dentistry)
Kim, Myung-Jin (Department of Periodontolog, Pusan National University School of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.41, no.2, 2011 , pp. 54-59 More about this Journal
Abstract
Purpose: The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis. Methods: In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog. Results: Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK). Conclusions: Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immuno-pathogenic mechanism of bacterial antigen-triggered autoimmune diseases.
Keywords
Antibodies; Periodontitis; Porphyromonas gingivalis;
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