• Korean Journal of Environmental Agriculture
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• v.32 no.2
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• pp.148-154
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• 2013

BACKGROUND: Genetically modified(GM) trigonal cactus(Hylocereus trigonus Saff.) contained a coat protein gene of cactus virus X (CVX), which conferred resistance to the virus, phosphinothricin acetyltransferase (bar) gene, which conferred herbicide resistance, and a cauliflower mosaic virus 35S promoter (CaMV 35S). This study was conducted to evaluate the possible impact of GM trigonal cactus cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM trigonal cactus cultivation soils. The total numbers of bacteria, and actinomycete in the rhizosphere soils cultivated GM and non-GM trigonal cactus were similar to each other, and there was no significant difference. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM trigonal cactus were Proteobacteria, Uncultured archaeon, and Uncultured bacterium. The denaturing gradient gel electrophoresis (DGGE) profiles show a similar patterns, significant difference was not observed in each other. DNA was isolated from soil cultivated GM and non-GM trigonal cactus, we analyzed the persistence of the inserted gene by PCR. Amplification of the inserted genes was not observed in the soil DNA, which was collected after harvest. CONCLUSION(S): This result suggests that the GM trigonal cactus cultivation does not change significantly the microbial community.

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.181-186
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• 2003

This study was performed to analyze quantitatively the number of living bacteria in forest soil samples collected from Mt. Keryong using improved direct viable count (DVC) and plate count (PC) methods. The number of living bacteria by DVC comprised 18～44% of the total direct count (TDC), whereas the number of living bacteria by PC was less than 1% of TDC. These results showed that viable but non-culturable (VBNC) bacteria existed in the soil with high percentages. Besides, DVC was proved to make it possible to make a quantitative detection of the VBNC bacteria. On the other hand, upon measuring the value from the conventional nutrient broth (NB) and $10^{-2}$ folded diluted nutrient broth (DNB), the values from the DNB showed 5 to 10 times higher than those from the conventional NB medium. These results indicate that oligotrophic bacterial groups, which could multiply in the low nutrient broth, abundantly exist in the soil ecosystem. It would also be possible to apply this kind of method to other substrate to make a quantitative detection of soil bacterial groups.

• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.335-344
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• 2021

BACKGROUND: Cyantraniliprole is a systemic diamide insecticide that has been used to control lepidopteran pests in agriculture. Cyantraniliprole has become an issue due to its potentiality of unexpectable contamination in rotational crop cultivation. Thus, studies on the evaluation of cyantraniliprole translocated from soil into rotational crops are required. METHODS AND RESULTS: Cyantraniliprole was treated at a yearly maximum application level onto bare soil under greenhouse conditions in two geographically different regions. Lettuce was transplanted and spinach and radish were sown onto the soil 30 and 60 days-plant back intervals (PBIs) after cyantraniliprole treatment. The QuEChERS method was modified and coupled with LC/MS/MS analysis to determine the residues of cyantraniliprole in soil and plant samples. The methods for sample preparation and instrumental conditions were validated to meet the criteria of Codex guidelines and were successful to determine cyantraniliprole quantitatively and qualitatively in the samples. Cyantraniliprole residues in lettuce samples were 0.01 mg/kg for PBI 60 and 0.02 mg/kg for PBI 30, respectively. The residues in spinach samples were 0.01 mg/kg for PBI 60 and 0.01~0.02 mg/kg for PBI 30, respectively. Less than limit of the quantitation (LOQ) level (0.01 mg/kg) of cyantraniliprole was observed in radish samples. The residues in the plant samples were found as the levels less than maximum residue limit (MRL) for leafy and root vegetables. CONCLUSION(S): This study suggests PBI 30~60 days for rotational cultivation of lettuce, spinach and radish in greenhouse soil treated with cyantraniliprole at a yearly maximum application level.

• Korean Journal of Environmental Agriculture
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• v.38 no.2
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• pp.110-118
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• 2019

BACKGROUND: Residual organochlorine pesticides (OCPs) are designated as persistent organic pollutants (POPs) by Stockholm Convention because they bioaccumulate through the food web, and pose a risk of causing adverse effect to human health and the environment. Apple and pear is economic crop in agriculture, and its cultivation area and yield has been increased. Therefore, we tried to investigate the OCPs residue in apple and pear orchard soils and fruits. METHODS AND RESULTS: Extraction and clean-up method were developed using the modified QuEChERS method for residual organochlorine pesticides (ROCPs) in apple and pear orchard soil and fruits. Recovery and limit of quantitation (LOQ) of ROCPs in soil and fruits were 75.4-101.4 and 76.9-93.4%, 0.03-0.21 and $0.6-1.2{\mu}g/kg$, respectively. Detected ROCPs in apple and pear orchard soil was 2,4-DDT, 4,4-DDD, 4,4-DDE, 4,4-DDT, and endosulfan sulfate, the residues were 2.2, 1.9-48.0, 1.3-84.1, 90.6-863.1, and $11.3-239.0{\mu}g/kg$, respectively. But five pesticides in all fruit samples were not detected. CONCLUSION: These results showed that ROCPs residues in apple and pear orchard soil had no effect on safety of agricultural products.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.1
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• pp.65-70
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• 2003

This research aims to classify wetland types and evaluate functions of a wetland created by abandoned rice paddies. The case study area is Ja-un wetland in Daejeon Metropolitan City. In this study, wetland types were classified based on the basic elements of wetlands such as hydrology, vegetation, and soil conditions. This study was carried out based upon field surveys, drawing maps and publication, and modified-RAM which can evaluate the general functions and conservation values of wetlands. The analysis shows that several types of wetlands were identified, such as Palustrine/ Perennial/ Hydrophytes, Palustrine/ Perennial/ Openwater, Palustrine/ Seasonal/Herbal, Palustrine/ Seasonal/ Shrub and Scrub, Palustrine/ Seasonal/ Hydrophye, and Riparian/ Seasonal/ Shrub and Scrub in the Ja-un wetland. The average level of functions of the wetlands is very "HIGH", and it is recommended that prompt conservation measures should be taken.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2005.04a
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• pp.393-399
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• 2005

The purpose of this study is to suggest some references of maintenance and design of concrete gravity dams by analyzing dynamic characteristics in x, y, z directions. It is considered as additional mass, soil interaction for numerical dynamic analysis for gravity concrete dams in Han River basin as some cases. The result shows that the overflow structure can be possibly underestimated for the evaluation of the seismic performance using seismic intensity, modified seismic intensity methods. A much more research is still necessary for the evaluation of comprehensive seismic performance of concrete gravity dam

• Geomechanics and Engineering
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• v.14 no.6
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• pp.509-517
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• 2018

This paper proposes a numerical methodology for capturing the complete curve of a pressuremeter test including initial or disturbed parts and loops through a stiffness-based approach adopted in three dimensional finite difference code, FLAC3D. In order to enable this, a new hyperbolic model was used to replace the conventional linear elastic model prior to peak strength of Mohr-Coulomb soil model and update or degradation of shear modulus was considered. Presented modeling approach and implemented constitutive model are impressively successful. It leads to obtain the whole set of parameters for characterizing sands and seems promising for modeling the most of geotechnical structures.

• Economic and Environmental Geology
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• v.33 no.6
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• pp.557-563
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• 2000

Subsidence hazard has never been considered seriously until recent yews in Korea, although its socioeconomic impact on Korea becomes more and more enormous. There have been a few studies for the application of GIS analysis technique to the prediction of subsidence hazard. For GIS analysis, several factors, which are represented by coverage, are considered and selected for building a GIS model. Numerical method was used to quantify the importance of each factor in GIS model and the result from numerical modeling using FLAC was compared with that from previous research based on empirical methods. Analysis in 3-D needs more computer resources (i.e. memory). Therefore that in 2.5-D was considered to overcome the problem. Not only maximum vertical subsidence but also maximum horizontal strain and maximum slope have been considered for the assessment of subsidence hazard. The model can be easily modified for the purpose of applications in any subsidence area, especially cavern or abandoned mines under thick soil layer.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.3
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• pp.409-413
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• 2006

The accumulated deformation due to the undrained creep causes the general stability problem for the overall soil mass. In this study, the time-dependent constitutive equation, into which a damage law, modified cam clay model, and Perzyna's generalized viscous theory were incorporated, was derived microscopically. The model prediction agreed well with the experimental result including the case of the undrained creep rupture.

• Journal of The Korean Society of Agricultural Engineers
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• v.48 no.7
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• pp.35-43
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• 2006

This study was carried out to investigate the liquefaction behaviour and predict deviator stress with matric suction, of unsaturated silty sand. The unsaturated soil tests were conducted using a modified triaxial cell and specimens were prepared using the moisture tamping method. The axis translation technique was used to create the desired matric suctions in the specimen. Undrained triaxial compression tests were carried out at matric suction of 0, 2, 5, 10 and 25 kPa. The specimens were sheared to axial strains of about 20% to obtain steady state conditions. The results showed that liquefaction of silty sand only occurs at matric suction of 0 kPa and 2 kPa. The results also show that at matric suctions of 5, 10 and 25 kPa, the resistance to liquefaction increases. As the suction increases, the undrained effective stress path approached the drained stress path. Also, the predicted and measured maximum deviator stress for unsaturated soils using the effective stress concept showed good agreement as matric suction increases. The deviator stress increase is nonlinear as matric suction increases.