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The Detection and a Quantitative Evaluation of Viable but Non-Culturable Soil Bacteria Using a Modified Direct Viable Count Method  

황경숙 (목원대학교 생명과학부 미생물학과)
양희찬 (목원대학교 생명과학부 미생물학과)
염곡효 (좌하대학 농학부 응용생물과학과)
Publication Information
Korean Journal of Microbiology / v.39, no.3, 2003 , pp. 181-186 More about this Journal
Abstract
This study was performed to analyze quantitatively the number of living bacteria in forest soil samples collected from Mt. Keryong using improved direct viable count (DVC) and plate count (PC) methods. The number of living bacteria by DVC comprised 18～44% of the total direct count (TDC), whereas the number of living bacteria by PC was less than 1% of TDC. These results showed that viable but non-culturable (VBNC) bacteria existed in the soil with high percentages. Besides, DVC was proved to make it possible to make a quantitative detection of the VBNC bacteria. On the other hand, upon measuring the value from the conventional nutrient broth (NB) and $10^{-2}$ folded diluted nutrient broth (DNB), the values from the DNB showed 5 to 10 times higher than those from the conventional NB medium. These results indicate that oligotrophic bacterial groups, which could multiply in the low nutrient broth, abundantly exist in the soil ecosystem. It would also be possible to apply this kind of method to other substrate to make a quantitative detection of soil bacterial groups.
Keywords
DNB (diluted nutrient broth); DVC (direct viable count); EB (ethidium bromide); oligotrophic bacteria; VBNC (viable but non-culturable) bacteria;
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