• Genomics & Informatics
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• v.11 no.1
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• pp.46-51
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• 2013

Normal-karyotype acute myeloid leukemia (NK-AML) is a highly malignant and cytogenetically heterogeneous hematologic cancer. We searched for somatic mutations from 10 pairs of tumor and normal cells by using a highly efficient and reliable analysis workflow for whole-exome sequencing data and performed association tests between the NK-AML and somatic mutations. We identified 21 nonsynonymous single nucleotide variants (SNVs) located in a coding region of 18 genes. Among them, the SNVs of three leukemia-related genes (MUC4, CNTNAP2, and GNAS) reported in previous studies were replicated in this study. We conducted stepwise genetic risk score (GRS) models composed of the NK-AML susceptible variants and evaluated the prediction accuracy of each GRS model by computing the area under the receiver operating characteristic curve (AUC). The GRS model that was composed of five SNVs (rs75156964, rs56213454, rs6604516, rs10888338, and rs2443878) showed 100% prediction accuracy, and the combined effect of the three reported genes was validated in the current study (AUC, 0.98; 95% confidence interval, 0.92 to 1.00). Further study with large sample sizes is warranted to validate the combined effect of these somatic point mutations, and the discovery of novel markers may provide an opportunity to develop novel diagnostic and therapeutic targets for NK-AML.

• Mass Spectrometry Letters
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• v.4 no.4
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• pp.71-74
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• 2013

In the present study, we report that the charge-directed (assisted) peptide dissociation products, such as b- and y-type peptide backbone fragments, were the major products in MS/MS and $MS^3$ applications of some o-TEMPO-Bz-C(O)-peptide ions, while radical-driven dissociation products, such as a/x and c/z-type fragments, were previously shown to be the major products in the free radical initiated peptide sequencing mass spectrometry (FRIPS MS). Those o-TEMPO-Bz-C(O)-peptides share a common feature in their sequences, that is, the peptides do not include an arginine residue that has the highest proton affinity among free amino acids. The appearance of b- and y-type fragments as major products in FRIPS MS can be understood in terms of the so-called "mobile-proton model". When the proton is highly mobilized by the absence of arginine, the chare-directed peptide dissociation pathways appear to be more competitive than the radical-driven dissociation pathways, in our FRIPS experiments.

• The Journal of Korean Association of Computer Education
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• v.12 no.3
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• pp.53-62
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• 2009

As Information communication technology develops, requirements for new educational media and new contents gets bigger and bigger. Especially PDA is required for educational media on m-learning environment. We design and implement intra-educational contents sequencing model and educational contents sequencing model between educational contents for PDA as educational supplementary media for m-learning environment, and LMS supporting PDA. And we balanced the work load of PDA and LMS and constructed practical service platform for using PDA as educational supplementary device.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.51 no.6
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• pp.265-270
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• 2002

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and application area. So, the improvement of DNA hybridization detection method is very important for the determination of this hybridization reaction. Several molecular biological techniques require accurate predictions of matched versus mismatched hybridization thermodynamics, such as PCR, sequencing by hybridization, gene diagnostics and antisense oligonucleotide probes. In addition, recent developments of oligonucleotide chip arrays as means for biochemical assays and DNA sequencing requires accurate knowledge of hybridization thermodynamics and population ratios at matched and mismatched target sites. In this study, we report the characteristics of the probe and matched, mismatched target oligonucleotide hybridization reaction using thermodynamic method. Thermodynamic of 5 oligonucleotides with central and terminal mismatch sequences were obtained by measured UV-absorbance as a function of temperature. The data show that the nearest-neighbor base-pair model is adequate for predicting thermodynamics of oligonucleotides with average deviations for $\Delta$H$^{0}$ , $\Delta$S$^{0}$ , $\Delta$G$_{37}$ $^{0}$ and T$_{m}$, respectively.>$^{0}$ and T$_{m}$, respectively.

• The Journal of the Korea Contents Association
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• v.4 no.1
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• pp.8-17
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• 2004

In this paper, we design an intelligent loaming management logics which provide personalized teaming considering adaptive learning content dement and content sequencing. We enhance the existing functional model including adaptive learning management functions. Also, we present a system architecture to implement the adaptive learning management system. We realize the adaptive teaming management system based on the SCORM run-time engine.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.33 no.3
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• pp.154-161
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• 2010

This paper considers the simultaneously firing model for the artillery operations. The objective of this paper is to find the optimal fire sequence minimizing the final completion time of the firing missions of multiple artillery units for multiple targets. In the problem analysis, we derive several solution properties to reduce the solution space. Moreover, two lower bounds of objective are derived and tested along with the derived properties within a branch-and-bound scheme. Two efficient heuristic algorithms are also developed. The overall performances of the proposed branch-and-bound and heuristic algorithms are evaluated through various numerical experiments.

• Journal of Korean Institute of Industrial Engineers
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• v.19 no.4
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• pp.41-48
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• 1993

Prime advantage of flexible manufacturing systems(FMS) is a flexibility. Flexibility is expected to prolong the service life of a manufacturing facility and enable it to respond quickly and economically to dynamic market change. The FMS loading decision is concerned with the allocation of operations and tools to machines subject to technological and capacity constraints of the system. Modern FMS loading problem has the multiple objectives such as processing cost, time and work load balance. We propose multi-objectives which could be used to formulate the loading/routeing problem and sequencing decision which should be adopted for each part type in order to maximize the machine flexibility by Hamming distance matrix based on Incidance matrix. Finally, a numerical example is provided to illustrate the proposed model.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.24 no.64
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• pp.21-28
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• 2001

This paper suggests a new heuristic to improve robotic cells efficiency with a finite buffer. An efficient heuristic for parts sequencing with this configuration is developed. Analyzing robot\\`s movements and defining the events for the completion times of robot\\`s segmental activities enable us to develop a mathematical model that can be used to estimate, the completion time in the robotic cell. The robotic cell is consisting of two computer numerical control machines and a finite buffer to manufacture multiple parts. The developed heuristic can be used to estimate an optimum or near optimum parts sequences for this configuration. Numerical examples are followed to show the validity of the heuristic.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04a
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• pp.106-108
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• 2003

Human Genome Project와 같은 대형 Sequencing 프로젝트와 High-throughput Sequencing 기술의 발전으로 현재 Expressed Sequence Tag (EST)와 같은 대량의 DNA 서열들이 생산되고 있다. 이를 효과적이고 효율적으로 분석해야 할 필요성이 증대되고 있다. 대부분의 실험자들이 서열 분석을 위해 우선적으로 BLAST 검색을 이용하고 있다. 하지만 대량의 서열, 검색 DB의 크기, BLAST 검색 결과의 복잡성에 의해 어려움을 겪고 있다. 이에 빠르고 정리된 결과를 보여줄 수 있는 BLAST 검색 시스템의 필요성이 커지고 있다. 이에 본 논문은 미국 생명공학연구소(NCBI)에서 제공하는 유전자 서열 검색 툴인 BLAST(Basic Logical Alignment Tool)를 클러스터 수퍼 컴퓨터 구축 기술을 기반으로 한 병렬처리와 Gene Ontology를 이용하여 방대한 양의 서열 검색 결과를 요약하는 모형을 제시한다. 이것은 신약개발 및 유전자 발굴 등의 연구기간을 획기적으로 단축시켜 신약 개 발, 농업, 화학, 의료, 환경 등 생명공학 연구에 핵심적인 역할을 할 수 있다. 또한 성능 실험을 통하여 분석결과 대기시간을 최소화하는 병렬처리모형의 효율성을 검증하였다.

• Journal of the Korea Safety Management & Science
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• v.3 no.3
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• pp.97-106
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• 2001

This paper considers automated storage and retrieval systems with double shuttle. We developed the expected travel time model based on the first come first served rule. An heuristic procedure by retrieval order sequencing was presented which aimed to improve the operation efficiency laking advantage of ＂No cost zone＂. Through sensitivity study, we evaluated the performance of the double shuttle system working on the four command cycle.