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Chemical Composition of Cultured and Wild Codonopsis lanceolata Roots of Different Age Groups -I. Proximate Composition, Minerals and Protein Fractions- (더덕(沙蔘)의 년근별(年根別) 화학성분(化學成分)에 관(關)한 연구(硏究) -제1보(第1報) : 일반성분(一般成分), 무기질(無機質) 및 단백질(蛋白質) 분획(分劃)-)

  • Park, Boo-Duck;Park, Yong-Gone;Choi, Kwang-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.3
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    • pp.274-279
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    • 1985
  • Proximate compositions, minerals and protein fractions of the roots of cultured and wild Codonopsis lanceolata of different age groups were examined as the basic research for the study of their source of processed foods. The most abundant proximate composition of the roots of C. lanceolata was observed to be total sugars and next come crude protein, crude fiber, crude fat and ash in descending order irrespective of cultured and wild ones. The richest mineral contained in the roots was noticed to be K and followed by Mg and Ca. Generally increased tendency of crude protein, fat, ash, K, Mg, Ca, Mn, Zn, Cu and P contents were observed with older roots, however, decreased total sugars and Fe content. Lead and cadmium content was far bellow the authorized tolerance limits. The quantitative fractionation of the protein of the roots ranked albumin the highest content, followed by globuin, prolamin and glutelin. Decreased albumin content was observed with the older age roots, while increased globulin, prolamin and glutelin content. The minimum solubility of the soluble protein of the roots was found to be at pH 4.0 and maximum, at pH 10.0. Disc gel electrophoresis of the soluble protein of C. lanceolata roots showed almost similar patterns and numbers of bands. The molecular weight for main band protein was estimated to be about 90,000.

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Studies on Processing Techniques in Barley II. The Processing and Cooking Quality of Cut-polished Barley in Naked Barley (보리의 가공기술 개선연구 II. 쌀보리의 할맥가공특성과 취반성)

  • Kim, Y.S.;Chang, H.K.;Park, N.P.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.287-291
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    • 1988
  • These studies were carried out to find out the polishing properties and cooking quality of the cut-polished barley. Naked barley, Youngsanbori which was produced in Chonnam province, Korea in 1981, was applied for this experiment. Polished barley was produced by the conventional method and cut-barley was manufactured by the method established by Wheat and Barley Research Institute. The yield of cut-polished barley was 68.2% and that of conventionally polished barley was 70.1 %. The ratio of length to width was 2.88 in cut-polished barley and that of conventionally polished barley was 1.36. And weight of 1,000 kernel was 9.5g in cut-polished barley and 18.5g in conventionally polished barley. Energy consumption was found to be 91.1kW/1,000kg in conventionally polished barley and 105kW/1,000kg in cut-polished barley. Whiteness, water uptake ratio and expanded volume of cooked barley were 45.5, 225.7 and 283% in conventionally polished barley and 49.5, 312.7 and 318% in cut-polished barley, respectively.

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Trace element concentrations profiles in the hair of normal children living in the northern area of Seoul (서울 북부지역에 거주하는 정상 아동의 모발 미네랄 함량)

  • Kwon, Ji Won;Kim, Byung Eui;Park, Mi Jung;Kim, Sang Woo
    • Clinical and Experimental Pediatrics
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    • v.49 no.1
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    • pp.18-23
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    • 2006
  • Purpose : The reliability of hair mineral analyses regarding nutritional status, environmental exposure or diseases is controversial. The aim of this study was to determine the normal reference range of hair mineral concentration of Korean children. Methods : We examined hair mineral concentrations of 223 children(3-12 yrs old, 110 boys, 113 girls, mean age $8.8{\pm}2.2$ yrs old) living in the northern area of Seoul. The trace elements including six toxic elements(Al, As, Cd, Ba, Hg, Pb) and 11 nutritional elements(Na, Mg, P, K, Ca, Cr, Mn, Fe, Cu, Zn, Se) were analyzed by inductive coupled plasma mass spectrometry(ICP-MS). Results : The mean concentrations of Ca and Mg were higher in girls than in boys. The mean concentrations of Cd, Pb and Cr were higher in boys than girls. The Zn, Ca, Mg, Cu and Hg levels in hair samples were positively correlated with increasing age. The Zn levels of the Korean children's hair samples appear to be lower than that of other countries' reference values. Conclusion : There are considerable differences in hair mineral concentrations by age, sex and race. Additional research is needed to establish Korean reference values, and to evaluate the usefulness of hair mineral analyses as a screening tool for nutrition- and environment-related childhood diseases.

Stability of the prosthetic screws of three types of craniofacial prostheses retention systems

  • Lanata-Flores, Antonio Gabriel;Sigua-Rodriguez, Eder Alberto;Goulart, Douglas Rangel;Bomfim-Azevedo, Veber Luiz;Olate, Sergio;de Albergaria-Barbosa, Jose Ricardo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.42 no.6
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    • pp.352-357
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    • 2016
  • Objectives: This study aimed to evaluate the stability of prosthetic screws from three types of craniofacial prostheses retention systems (bar-clip, ball/O-ring, and magnet) when submitted to mechanical cycling. Materials and Methods: Twelve models of acrylic resin were used with implants placed 20 mm from each other and separated into three groups: (1) bar-clip (Sistema INP, São Paulo, Brazil), (2) ball/O-ring (Sistema INP), and (3) magnet (Metalmag, São Paulo, Brazil), with four samples in each group. Each sample underwent a mechanical cycling removal and insertion test (f=0.5 Hz) to determine the torque and the detorque values of the retention screws. A servo-hydraulic MTS machine (810-Flextest 40; MTS Systems, Eden Prairie, MN, USA) was used to perform the cycling with 2.5 mm and a displacement of 10 mm/s. The screws of the retention systems received an initial torque of 30 Ncm and the torque values required for loosening the screw values were obtained in three cycles (1,080, 2,160, and 3,240). The screws were retorqued to 30 Ncm before each new cycle. Results: The sample was composed of 24 screws grouped as follows: bar-clip (n=8), ball/O-ring (n=8), and magnet (n=8). There were significant differences between the groups, with greater detorque values observed in the ball/O-ring group when compared to the bar-clip and magnet groups for the first cycle. However, the detorque value was greater in the bar-clip group for the second cycle. Conclusion: The results of this study indicate that all prosthetic screws will loosen slightly after an initial tightening torque, also the bar-clip retention system demonstrated greater loosening of the screws when compared with ball/O-ring and magnet retention systems.

Ingredients Analysis and Biological Activity of Fermented Angelica gigas Nakai by Mold (곰팡이 발효 참당귀의 유효성분 분석 및 생리활성 작용)

  • Cha, Jae-Young;Kim, Hyun-Woo;Heo, Jin-Sun;Ahn, Hee-Young;Eom, Kyung-Eun;Heo, Su-Jin;Cho, Young-Su
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1385-1393
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    • 2010
  • Three mold strains, Aspergillus oryzae (AOFAG), Aspergillus kawachii (AKFAG), and Monascus purpureus (MPFAG) were used for fermentation of Angelica gigas Nakai powder. The contents of polyphenolic compounds, flavonoids, minerals, decursin and decursinol angelate and the activities of DPPH (${\alpha},{\alpha}'$-diphenyl-$\beta$-picrylhydrazyl) free radical scavenging, reducing power, and tyrosinase were measured. The highest contents of phenolic compound and flavonoid were NFAG at 2.78% and MPFAG at 1.18%, respectively. Major minerals were K, Mg, Fe, Na and Ca. Decursin and decursinol angelate were the major ingredients of Angelica gigas according to HPLC analysis. Decursin area was higher in all fermented Angelica gigas than in NFAG. The activities of free radical scavenging and tyrosinase were stronger in all fermented Angelica gigas than NFAG. However, the Fe/Cu reducing powers were stronger in NFAG than all fermented Angelica gigas. Overall, these results may provide the basic data needed to understand the biological activities and chemical characteristics of Angelica gigas fermented by mold for the development of functional foods.

Characteristics of Vanadium Leaching from Basaltic Soils of Jeju Island, Korea (제주도 현무암 기원 토양의 바나듐 용출 특성)

  • Hyun, Ik-Hyun;Yang, Cheol-Shin;Yun, Seong-Taek;Kim, Horim;Lee, Min-Gyu;Kam, Sang-Kyu
    • Journal of Environmental Science International
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    • v.25 no.11
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    • pp.1541-1554
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    • 2016
  • To understand the characteristics of vanadium leaching from soils formed by the weathering of basalts, paleo soil at Gosan, Jeju Island, Korea, and several present-day soils from neighboring areas were collected. Leaching experiments were carried out by two approaches: 1) batch experiments under various geochemical conditions (redox potential (Eh) and pH) and 2) continuous leaching experiments under conditions similar to those of natural environments. From the batch experiments, leached vanadium concentrations were highest under alkaline (NaOH) conditions, with a maximum value of $2,870{\mu}g/L$, and were meaningful (maximum value, $114{\mu}g/L$) under oxidizing ($H_2O_2$) conditions, whereas concentrations under other conditions (acidic-HCl, $neutral-NaHCO_3$, and $reducing-Na_2S_2O_3$) were negligible. This indicated that the geochemical conditions, in which soil-water reactions occurred to form groundwater with high vanadium concentrations, were under alkaline-oxidizing conditions. From the continuous leaching experiments, the pH and leached vanadium concentrations of the solution were in the ranges of 5.45~5.58 and $6{\sim}9{\mu}g/L$, respectively, under $CO_2$ supersaturation conditions for the first 15 days, whereas values under $O_2$ aeration conditions after the next 15 days increased to 8.48~8.62 and $9.7{\sim}12.2{\mu}g/L$, respectively. Vanadium concentrations from the latter continuous leaching experiments were similar to the average concentration of groundwater in Jeju Island ($11.2{\mu}g/L$). Furthermore leached vanadium concentrations in continuous leaching experiments were highly correlated with pH and Al, Cr, Fe, Mn and Zn concentrations. The results of this study showed that 1) alkaline-oxidizing conditions of water-rock (soil) interactions were essential to form vanadium-rich groundwater and 2) volcanic soils can be a potential source of vanadium in Jeju Island groundwater.

Study on the Mineral and Heavy Metal Contents in the Hair of Preschool Aged Autistic Children (자폐아동 모발에서의 미네랄 및 중금속 함량에 관한 연구)

  • Jung, Myung-Ae;Jang, Hyun-Seo;Park, Eun-Ju;Lee, Han-Woo;Choi, Jeong-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.11
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    • pp.1422-1426
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    • 2008
  • The purpose of this study was to test characteristics between normal and autistic children via comparison of nutrient intakes, hair mineral, blood free radical, and serotonin contents. A total of 50 children aged 3-9 were divided into two main groups of normal control children (n=22) and autistic children (n=28) tested by child psychiatrist. The nutrient intakes by 24-hour recall method were no significantly different between the two groups. The concentrations of toxic mineral, such as cadmium (Cd) and lead (Pb) in hair of autistic children were significantly higher, while concentration of antioxidant mineral (Cu, Zn, Fe) was lower than that of normal children. The autistic children had significantly higher concentrations of blood free radical than that of normal children. No significant difference was observed in serotonin concentration between the two groups. Our results suggest a possible role of increased toxic mineral and free radical, both of which may be relevant to the pathophysiology of autism in children with developmental delay.

Cloning and Characterization of a Novel Mannanase from Paenibacillus sp. BME-14

  • Fu, Xiaoyu;Huang, Xiaoluo;Liu, Pengfu;Lin, Ling;Wu, Gaobing;Li, Chanjuan;Feng, Chunfang;Hong, Yuzhi
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.518-524
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    • 2010
  • A mannanase gene (man26B) was obtained from a sea bacterium, Paenibacillus sp. BME-14, through the constructed genomic library and inverse PCR. The gene of man26B had an open reading frame of 1,428 bp that encoded a peptide of 475- amino acid residues with a calculated molecular mass of 53 kDa. Man26B possessed two domains, a carbohydrate binding module (CBM) belonging to family 6 and a family 26 catalytic domain (CD) of glycosyl hydrolases, which showed the highest homology to Cel44C of P. polymyxa (60% identity). The optimum pH and temperature for enzymatic activity of Man26B were 4.5 and $60^{\circ}C$, respectively. The activity of Man26B was not affected by $Mg^{2+}$ and $Co^{2+}$, but was inhibited by $Hg^{2+},\;Ca^{2+},\;Cu^{2+},\;Mn^{2+},\;K^+,\;Na^+$, and $\beta$-mercaptoethanol, and slightly enhanced by $Pb^{2+}$ and $Zn^{2+}$. EDTA did not affect the activity of Man26B, which indicates that it does not require divalent ions to function. Man26B showed a high specific activity for LBG and konjac glucomannan, with $K_m,\;V_{max}$, and $k_{cat}$ values of 3.80 mg/ml, 91.70 ${\mu}mol$/min/mg protein, and 77.08/s, respectively, being observed when LBG was the substrate. Furthermore, deletion of the CBM6 domain increased the enzyme stability while enabling it to retain 80% and 60% of its initial activity after treatment at $80^{\circ}C$ and $90^{\circ}C$ for 30 min, respectively. This finding will be useful in industrial applications of Man26B, because of the harsh circumstances associated with such processes.

Designed of rPP/d2w®/ZnO Nanocomposite Flexible Film for Food Packaging and Characterization on Mechanical and Antimicrobial Properties (산화분해촉매를 함유한 rPP/ZnO 나노컴포지트 유연식품포장필름 제조 및 물성 특성 연구)

  • Lee, Jin-kyoung;Gil, Bo-min;Lee, Dong-jin;Lee, Ik-mo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.24 no.1
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    • pp.1-11
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    • 2018
  • In this study, pro-oxidant($d2w^{(R)}$) and rPP/ZnO nanocomposite flexible films for food packaging were prepared, and their mechanical and antimicrobial properties were investigated. As a result, the carbonyl index and hydroxyl index increased with exposured time to heat and UV rays. Surface analysis showed that the addition of zinc oxide improved the dispersibility and compatibility of the polymer, so that the surface of the composite film was smooth and the zinc oxide particles were smaller than the compared film. And it kept the physical properties by heat and UV ray blocking effect, and it worked to reduce decomposition. In the antimicrobial activity test, the microbial reduction rate was 3 logs or more at the use concentration of zinc oxide. The tensile strength was increased and the elongation was decreased. Oxidative degradability of multi-layered film in UV exposured for 72 hours, the molecular weight of the film decreased by 75.6%, 1,294 g/mol Mn and 5,920 g/mol Mw. In the safety analysis of food packaging materials, we obtained that are in standard of polypropylene, a food contact material of domestic law.

The Detection of Magnetic Properties in Blood and Nanoparticles using Spin Valve Biosensor (스핀밸브 바이오 센서를 이용한 혈액과 나노입자의 자성특성 검출)

  • Park, Sang-Hyun;Soh, Kwang-Sup;Ahn, Myung-Cheon;Hwang, Do-Guwn;Lee, Sang-Suk
    • Journal of the Korean Magnetics Society
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    • v.16 no.3
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    • pp.157-162
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    • 2006
  • In this study, a high sensitive giant magnetoresistance-spin valve (GMR-SV) bio-sensing device with high linearity and very low hysteresis was fabricated by photolithography and ion beam deposition sputtering system. Detection of the Fe-hemoglobin inside in a red blood and magnetic nanoparticles using the GMR-SV bio-sensing device was investigated. Here a human's red blood includes hemoglobin, and the nanoparticles are the Co-ferrite magnetic particles coated with a shell of amorphous silica which the average size of the water-soluble bare cobalt nanoparticles was about 9 nm with total size of about 50 nm. When 1 mA sensing current was applied to the current electrode in the patterned active GMR-SV devices with areas of $5x10{\mu}m^2 $ and $2x6{\mu}m^2 $, the output signals of the GMRSV sensor were about 100 mV and 14 mV, respectively. In addition, the maximum sensitivity of the fabricated GMR-SV sensor was about $0.1{\sim}0.8%/Oe$. The magnitude of output voltage signals was obtained from four-probe magnetoresistive measured system, and the picture of real-time motion images was monitored by an optical microscope. Even one drop of human blood and nanopartices in distilled water were found to be enough for detecting and analyzing their signals clearly.