• Journal of Wetlands Research
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• v.23 no.4
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• pp.352-363
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• 2021

The purpose of this study was to evaluate the load of nutrients contained in the drainage discharged from the facility horticultural complex and to use them for re-use of fluids and design for introduction of water treatment plants. Representative hydroponic cultivation crops were selected as tomato, paprika, cucumber, and strawberry, and the total number of samples analyzed for water quality was 80. As a result of the analysis, since various fertilizer components such as N, P, K⁺, Na⁺, Mg²⁺, Ca²⁺, Si₄⁺, HCO₃^-, Cl^-, S^2-, Fe, Mn, Cu, Zn, Mo and B are contained at very high concentrations in the drainage, the need for water treatment was confirmed. Through statistical analysis, it was analyzed that the drainage concentration of strawberries was lower than that of tomatoes, paprika, and cucumbers. In the case of tomatoes, these essential ion concentrations are the highest, so it was confirmed that they are subject to valuable resources in terms of reuse of fertilizers. The load of N and P of the drainage discharged from the facility horticultural complex 1m² was analyzed. For N, the daily processing capacity of 4.0 kg of tomatoes, 3.3 kg of paprika, 3.0 kg of cucumbers, and 1.5 kg of strawberries was calculated based on 1 ha. It was suggested that the P concentration needs a scale and capacity that can handle 0.5 kg of tomatoes, 0.6 kg of paprika, 0.4 kg of cucumber, and 0.2 kg of strawberries per day. Through this study, the amount of nitrogen and phosphorus contained in the drainage discharged from the greenhouse of each crop was evaluated to analyze the economy. In addition, it was expected to be used as basic data that can be used to calculate the treatment capacity to be reflected when introducing water treatment facilities in facility horticultural complexes for sustainable agriculture.

• Asian Journal of Atmospheric Environment
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• v.6 no.2
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• pp.73-82
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• 2012

This study was aimed to thoroughly estimate the characteristics of indoor particulate matter (PM) collected on subway platforms by the cooperative approach of semi-bulk and single particle analyses. The size-resolved PM and its number concentration were measured on the platform in a heavily traveled subway station in Fukuoka, Japan. Particle Induced X-ray Emission (PIXE) and micro-PIXE techniques were applied to the chemical analyses of semi-bulk and single particle, respectively. There was the close resemblance of timely fluctuation between PM number concentration and train service on the third basement floor (B3F) platform compared to the second basement floor (B2F) and its maximum level was marked in rush hour. Higher number counts in large particles ($>1{\mu}m$) and lower number counts in fine particles ($<1{\mu}m$) were shown on the platform compared to an above ground. PM2.5 accounted for 58.2% and 38.2 % of TSP on B3F and on B2F, respectively. The elements that were ranked at high concentration in size-resolved semi-bulk PM were Fe, Si, Ca, S, and Na. The major elements tending to have more elevated levels on B3F than B2F were Fe (4.4 times), Ca (17.3 times), and Si (46.4 times). Although concentrations were very low, Cr ($11.9ng\;m^{-3}$ on B3F, $2.4ng\;m^{-3}$ on B2F), Mn ($3.4ng\;m^{-3}$ on B3F, $0.9ng\;m^{-3}$ on B2F), and Pb ($0.6ng\;m^{-3}$ on B3F, $1.6ng\;m^{-3}$ on B2F) were detected from PM2.5. Individual PM was nearly all enriched in Fe with Si and Ca. Classifying and source profiling of the individual particles by elemental maps and particle morphology were tried and particles were presumably divided into four groups (i.e., train/rail friction, train-rail sparking, ballast/abrasive, and cement).

• Journal of fish pathology
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• v.14 no.1
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• pp.31-36
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• 2001

A novel clonidine binding sites were characterized in the intestinal membrane isolated from seawater eels, Anguilla japonica. The specific clonidine binding sites consisted of at least two classes, high affinity ($K_d=1.4{\pm}0.3$ nM n = 5) and low affinity ($K_d=175{\pm}34$ nM n = 5) sites. The specific binding of 2 nM [$^3H$]clonidine was most enhanced at $20^{\circ}C$ and pH 7.5, and reversed by unlabelled clonidine. Such binding was hardly inhibited by adrenaline, yohimbine or rauwolscine, indicating that most binding sites are distinct from $\alpha_2$-adrenoceptor. The specific clonidine binding sites was inhibited by various imidazoline/guanidinium drugs, indicating existence of imidazoline/guanidinium receptive sites (IGRS) or imidazoline receptors in the eel intestine. Competition experiments revealed that rank order to displace 2 nM [$^3H$]clonidine from their binding sites was as follows : guanabenz > cirazoline = naphazoline = UK14,304 = ST587 $\geq$ clonidine $\geq$ idazoxan = RX821002 = tolazoline > ST93 = oxymetazoline = amiloride = ST91 > yohimbine = efaroxan = rauwolscine $\geq$ adrenaline = ST567 = histamine = agmatine. Although physiological role of IGRS is not clear yet even in mammalian cell/tissues, eel intestine may be a good model to elucidate how the IGRS act in the cell and to decide what is the endogenous ligand for the IGRS.

• Microbiology and Biotechnology Letters
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• v.24 no.1
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• pp.9-18
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• 1996

Streptococcus mutans has been implicated as primary causative agents of dental caries by insoluble glucan (IG) in human and experimental animals. An attempt was made to search for the ${\alpha}$-1,3 glucanase that degrades IG produced by S. mutans. ${\alpha}$-1,3 glucanase was detected in the culture supernatant of microorganisms, which are isolated from soils on agar medium containing IG as a sole carbon source. This Streptomyces sp. hydrolysed IG produced by immobilized S. mutans and was named as Y9373. This enzyme required ${\alpha}$-1,3 glucan (IG) as an inducer. The optimum conditions for enzyme production were studied. The enzyme was purified by 30~70% $(NH_4)_2SO_4$ precipitation, anion exchange chroma tography on DEAE-cellulose and gel filtration on Sepadex G-75. The purified enzyme has a specific activity of 7840.0 U/mg protein giving 32.1-fold purification and final yield of 0.53%. The molecular weight was estimated to be about 22.5 kDa by SDS-PAGE. The optimum pH and temperature for enzyme reaction were 6.5 and 37$^{\circ}C$, respectively and the enzyme was relatively stable at the temperature below 60$^{\circ}C$. The activity of purified enzyme was enhanced by adding $Co^{2+},\;Mn^{2+}\;and\;Mg^{2+}$ into the medium, whereas inhibited by adding $Hg^{2+},\;Zn^{2+}$ and SDS. The $K_m\;and\;V_{max}$ value of ${\alpha}$-1,3 glucanase for IG were estimated to be 2.50 mM and 0.0431 mM/min, respectively. The thin layer chromatographic analysis of hydrolysates from IG with ${\alpha}$-1,3 glucanase showed that glucose was the main product of reaction. This enzyme activity was about 14 times higher than marketing dextranase as preventive agent against artificial dental caries by S. mutans in TH medium including 5% sucrose after 30 minutes.

• Korean Journal of Microbiology
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• v.52 no.3
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• pp.254-259
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• 2016

Acinetobacter sp. B-W producing siderophore, 2, 3-dihydroxybenzoic acid (DHB) was analyzed for plasmid content. Strain B-W harbored plasmid of 20 kb in size. Growth at $43^{\circ}C$ was effective in producing mutant cured of plasmid of strain B-W. This mutant lost the ability to produce 2, 3-DHB. Formation of siderophore halos on the chrome azurol S (CAS) agar medium was not detected by cured strain B-W. pHs of supernatants of wild type strain B-W and cured mutant grown in glucose and $MnSO_4$ containing medium at $28^{\circ}C$ for 3 days were 4.5 and 8.5, respectively. Antibiotic resistance against ampicillin, actinomycin D, bacitracin, lincomycin, and vancomycin was lost in cured mutant. Plasmid curing of strain B-W resulted in drastic reduction of minimal inhibitory concentration (MIC) of several antibiotics. E. coli $DH5{\alpha}$ was transformed with plasmid isolated from strain B-W. The transformant E. coli $DH5{\alpha}$ harbored a plasmid of the same molecular size as that of the donor plasmid. Transformant E. coli $DH5{\alpha}$ produced 2, 3-DHB and contained antibiotic resistant ability. Thus a single plasmid of 20 kb seemed to be involved in 2, 3-DHB production. Genes encoding resistance to antibiotics were also supposed to be located on this plasmid.

• Journal of Soil and Groundwater Environment
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• v.14 no.4
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• pp.33-44
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• 2009

Water curtain of an underground LPG storage cavern is a facility to prevent leakage of high pressure gases, for which groundwater should flow freely towards the cavern and groundwater level also must be stably maintained. In this study, in order to evaluate qualities of seepage water and surrounding groundwater of an underground LPG storage cavern in Yeosu, 4 rounds of samplings, field measurements and laboratory analyses (February, May, August, October of 2007) were conducted. According to field measurements, pH was weak acidic to neutral but it gradually increased with time. Electrical conductivity (EC) of groundwater near a salt stack showed very high values between 10.47 and 38.50 mS/cm. Dissolved oxygen (DO) showed a very wide range of 0.20~8.74 mg/L and a mean of oxidation-reduction potential (ORP) was 159 mV, which indicated an oxidized condition. Levels of $Fe^{2+}$ and $Mn^{2+}$ were mostly less than 3 mg/L. All of seepage waters showed a Na-Cl type while only groundwater near the salt stack showed a Na-Cl type with a high total dissolved solid. The other groundwaters exhibited typical $Ca-HCO_3$ types. Levels of aerobic bacteria were mostly very high (573-39,520 CFU/mL). Based on the analyses of these hydrochemistry and biological characteristics, it is concluded that there are no particular problems in groundwater and seepage water, which not causing a trouble in the cavern operation. However, both for control of bio-clogging and for sustainable operation of the water curtain system, a regular hydrochemical and microbiological monitoring is required for the seepage water and surrounding groundwater.

• Korean Journal of Ecology and Environment
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• v.45 no.4
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• pp.459-473
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• 2012

This study evaluated the rainfall-runoff characteristics of Non-point Pollution Source (NPS) of the impervious area through on-site monitoring. In this study, trend analysis was performed by various runoff analysis method of non-point pollution source. The characteristics of rainfall at impervious area appeared to be influenced by rainfall strength. It is judged that the measure is required to be prepared against that now that concentration difference of non-point pollution source appeared to be big by precedent number of days of no rainfall. However, it appeared that Rainfall Sustaining Time (RST) has nothing to do with effluent concentration of non-point pollution source, however, the rising tendency that effluent concentration did not appear because the tendency that concentration of non-point pollution source reduces more than 50% within initial 60 min due to first flushing effects and rainfall sustaining time is long. If looking into the outflow tendency of non-point pollution source at the impervious area, it showed the tendency that the concentration lowers gradually as time goes by after initial concentration appeared very high. However, it could be recognized that the concentration of non-point pollution source appeared to be high as the pollutants integrated on the surface of the road during dry season. The Event Mean Concentrations (EMCs) in impervious area were ranged $9.2{\sim}199.3mg{\cdot}L^{-1}$ for TSS, $8.1{\sim}24.2mg{\cdot}L^{-1}$ for $COD_{Mn}$, $0.070{\sim}1.860mg{\cdot}L^{-1}$ for T-N. Based on such runoff characteristics of non-point pollution source, it is judged that it would be desirable to process initial rain efficiently as the measure against initial rain phenomenon at the impervious area.

• Journal of environmental and Sanitary engineering
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• v.15 no.4
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• pp.59-77
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• 2000

This study was carried out to investigate on several factors, which contaminative the water quality through the water pipe during feeding water, in 42 largescaled apart-ments(total 84 cases) and assayed the Volatile Organic Compounds(VOCs) and concen-tration of heavy metals that inflow and outflow in reservior water in Jeonnam area(Mokpo, Suncheon, Yeosu) from January 1999 to December 1999. The results obtained were summarized as follows ; 1. The quality of the water pipe composition in the order of frequency in the quality of water pipes were Copper(45.2%)> Zinc(38.9%)> Stainless steel(9.5%)> PVC(4.8%)> PM(2.4%) in observing 42 sites. All of the drain pipes were used the cast iron quality. 2. The result of certification curve from 12 items(17kind) of VOCs was $1.0-4.0{\mu{g}}/{\ell}$ range, a coefficient of correlation was shown 0.99 over. A MDL of each substance range was within $0.1-1.0{\mu{g}}/{\ell}$. 3. The result of the assay, 5 kinds(Viny chloride, Dichloromethane, Ethylbenzene, M,P-xylene, Styrene) of the VOCs of 14 kinds was not detected and the other items were detected slightly. The detection rate of one item and over in total VOCs samples, were 25.9% in inflow and 27.9% in outflow. And frequency of detect in inflow/outflow of THM(Chloroform, Bromodichloro-methane, Dibromochloromethane, Bromoform) were shown higher than 94.1%, 97.0% each stages. It comes to the conclusion that all of the samples were reason able for drinking water standards. 4. The coefficient of correlation were reasonable, it shown 0.999 over in $0.1-1.0{\mu{g}}/{\ell}$ of a measuring range conditions of 4kinds in organic substance(Zn, Cu, Fe, Mn). 5. The results were showed suitability in 78 cases(92.9%) and unsuitability in 6 cases (7.1%), in 84 cases of in organic substances. Compare to inflow stage, mean concentrations of heavy metal, were increased slightly in Zn, Cu, Fe except Mn than outflow stage. The result of the mean concentration in organic substance inflow and outflow in the apartment water tank using Pair-compared T-test, in 95% reliance index, were $0.179mg/{\ell}(0.151-0.307mg/{\ell})$ in Zinc, $0.136mg/{\ell}(0.113-0.230mg/{\ell})$ in Copper, $0.052mg/{\ell}(0.048-0.098mg/{\ell})$ in Fe, and there was a bit growing tendency but there was no differece in Mn. 6. The mean concentration of Copper which used Cu pipe as a water supply pipe in apartment were $0.216mg/{\ell}(0.161-0.338mg/{\ell})$ in case of the Zine pipe were $0.286mg/{\ell}(0.204-0.435mg/{\ell})$. It shows that the detection rate was more higher than the other material used in Cu or Zn as the water supply pipe. We supposed to Cu and Zn substance were gushing out water supply pipe.

• The korean journal of orthodontics
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• v.39 no.5
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• pp.300-309
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• 2009

Objective: The purpose of this study was to evaluate the correlation between menton deviation and dental compensation in facial asymmetry. Methods: Tooth axis and distance of first molar and canine to the reference plane were investigated by cone-beam computerized tomography. The subjects consisted of 50 patients with asymmetric mandibles (male 21, female 29, mean age 24.3 years). Control groups were also assessed (male 11, female 9, mean age 25.6 years). Nine measurements (5 linear measurements and 4 angular measurements) were measured in order to evaluate the correlation between menton deviation and the linear and angular difference of first molar and canine in the deviated and none-deviated sides using the defined MPR images. The differences between deviated and non-deviated side, according to menton deviation, were statistically analyzed using stepwise multiple regression analysis. Results: From the result, Menton deviation was negatively correlated with mandibular first molar's angular measurement (${\Delta\angle}LM6$-Mn plane (dev.-ndev.)) and positively with maxillary fist molar's angular measurement (${\Delta\angle}UM6$-FH plane (dev.-ndev.)) (p < 0.01). Two angular measurements (${\Delta\angle}LM6$-Mn plane (dev.-ndev.), ${\Delta\angle}UM6$-FH plane (dev.-ndev.)) explained the variability in menton deviation with a significant $r^2$ value of 0.589. Conclusions: This study suggests that the tooth axis of upper and lower first molars leans towards the deviated side of Menton when there is mandibular asymmetry with Menton deviation.

• Economic and Environmental Geology
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• v.41 no.1
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• pp.63-79
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• 2008

Physicochemical characteristics of stream water, leachate, mine water and groundwater were investigated to estimate the influences of the tailing and waste rock from the abandoned Uljin mine area. Total extraction analysis and mineralogical studies were carried out to understand sulfide weathering and to determine the distributions of trace elements in the soil affected by mine waste (tailing, waste rock and leachate). The pH and EC value of the leachate from the tailing disposal ranged 2.9-6.0, $99{\sim}3,990{\mu}S/cm$, respectively, and the concentrations of dissolved major (up to 492 mg/l Ca; 83.8 mg/l Mg; 45.2 mg/l Na; 44.7 mg/l K, 50.8 mg/l Si) and trace elements (up to $826,060{\mu}g/l$ Fe; $131,230{\mu}g/l$ Mn; $333,600{\mu}g/l$ Al; $61,340{\mu}g/l$ Zn; $2,530{\mu}g/l$ Cu; $573{\mu}g/l$ Cd; $476{\mu}g/l$ Pb) were relatively high. The stream water showed the variation of dissolved metal concentrations in seasonally and spatially. The dissolved metal contents of the stream water increased by influx the leachate from the tailing disposal, but these of the down stream have been considerably decreased by mixing of dilute tributaries. The dissolved metal concentrations of the stream water at dry season (as February) were lower than these at rainy season (as May and July). These represent that the amounts of the leachate varied with season. However, stream water could not be effectively diluted by confluence with uncontaminated tributaries, because the flux of tributaries and streams reduced at dry season. Thus attenuations by dilution had been dominantly happened in rainy seasons. The order of accumulations of trace element in soils compared with background values revealed Mn>Fe>Pb>Cu>Zn. Sulfide minerals were mainly pyrrhotite, sphalerite and galena and chalcopyrite. Pyrrhotite was rapidly weathered along the edge and fractures, and results in the formation of Fe-(oxy)hydroxides, which absorbed a little amount of Zn.