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http://dx.doi.org/10.7845/kjm.2016.6043

Effect of plasmid curing on the 2, 3-dihydroxybenzoic acid production and antibiotic resistance of Acinetobacter sp. B-W  

Kim, Kyoung-Ja (Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University)
Kim, Jin-Woo (Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University)
Yang, Yong-Joon (Department of Plant and Food Science, Sangmyung University)
Publication Information
Korean Journal of Microbiology / v.52, no.3, 2016 , pp. 254-259 More about this Journal
Abstract
Acinetobacter sp. B-W producing siderophore, 2, 3-dihydroxybenzoic acid (DHB) was analyzed for plasmid content. Strain B-W harbored plasmid of 20 kb in size. Growth at $43^{\circ}C$ was effective in producing mutant cured of plasmid of strain B-W. This mutant lost the ability to produce 2, 3-DHB. Formation of siderophore halos on the chrome azurol S (CAS) agar medium was not detected by cured strain B-W. pHs of supernatants of wild type strain B-W and cured mutant grown in glucose and $MnSO_4$ containing medium at $28^{\circ}C$ for 3 days were 4.5 and 8.5, respectively. Antibiotic resistance against ampicillin, actinomycin D, bacitracin, lincomycin, and vancomycin was lost in cured mutant. Plasmid curing of strain B-W resulted in drastic reduction of minimal inhibitory concentration (MIC) of several antibiotics. E. coli $DH5{\alpha}$ was transformed with plasmid isolated from strain B-W. The transformant E. coli $DH5{\alpha}$ harbored a plasmid of the same molecular size as that of the donor plasmid. Transformant E. coli $DH5{\alpha}$ produced 2, 3-DHB and contained antibiotic resistant ability. Thus a single plasmid of 20 kb seemed to be involved in 2, 3-DHB production. Genes encoding resistance to antibiotics were also supposed to be located on this plasmid.
Keywords
Acinetobacter sp. B-W; antibiotic resistance; 2, 3-dihydroxybenzoic acid; plasmid curing;
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