• Title/Summary/Keyword: Mixed Soil

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Aerobic Liquid Fermentation of Residual Food Waste by Thermophilic Bacteria (고온세균을 이용한 남은 음식물의 호기적 액상발효)

  • Ryu, Seung-Yong;Park, Myoung-Ju;Kim, So-Young;Lee, Ki-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.3
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    • pp.126-131
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    • 2002
  • For the probiotic feed production from residual food waste, aerobic liquid fermentation was conducted by thermophilic bacteria. 11 Strains of bacteria were isolated from several soil sources and residual food waste. Screening was carried by shaking incubator for the separation of thermophilic strain at $55^{\circ}C$. The isolated strains were tested for enzyme activities such as ${\alpha}$-amylase and protease. 6 Bacterial strains were chosen and were adapted by repeated fermentation processes in food waste substrate. The viable cell count of them at final fermentation stages were shown as $3-7{\times}10^9/ml$ in 2L-jar fermenter. Among them B3, B6 showed higher enzyme activity. By the mixed fermentation of B3, B6 and Bacillus stearothermophilus, the highest viable cell count reached to $1.4{\times}10^{10}/ml$ in 8 hours.

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Engineering Characteristics of Coal Ash from Thermal Power Plants (화력발전소(火力發電所)에서 부산(副産)되는 석탄회(石炭灰)의 공학적(工學的) 특성(特性))

  • Chun, Byung Sik;Cho, Young Ku
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.8 no.2
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    • pp.59-66
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    • 1988
  • At the present time, annually about 2 million tons of coal ashes are generated from thermal power plants in Korea, however, they are dumped into ash ponds mixed with sea water very expensively. In this thesis, engineering characteristics of bituminous and anthracite ashes are studied to utilize them as construction materials. The coal ash is non-plactic material and its grain size falls in the range of silt, but it has better soil engineering characteristics than general soils of same grain size. For example, the permeability, shearing strength, CBR, and consolidation properties match to that of sandy soils, moreover, strengthening by hydration can be expected with the lapse of year because of CaO presence in the components. So, utilizing those coal ashes in a productive way as reclamation or banking materials is expected.

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Treatment of Acid Mine Drainage Using Immobilized Beads Carrying Sulfate Reducing Bacteria (황산염환원균 고정화 담체를 이용한 산성광산배수 처리)

  • Kim, Gyoung-Man;Hur, Won;Baek, Hwan-Jo
    • Economic and Environmental Geology
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    • v.41 no.1
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    • pp.57-62
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    • 2008
  • The application of constructed subsurface-flow wetlands for treatment of wastewater from abandoned mines is being increased. Crushed limestone, oak chips, and mushroom composites are often employed in a bulk form, as the substrates in the bed media. Efficiency of the subsurface-flow treatment system drops with time as the hydraulic conductivity of the wetland soil decreases significantly, presumably due to chemical reactions with the wastewater. The purpose of this study is to investigate the applicability of immobilized beads carrying sulfate reducing bacteria for acid mine drainage treatment system. The ingredients of immobilized beads are organic materials such as mushroom composite and oak chips, limestone powder for a pH buffer, mixed with a modified Coleville Synthetic Brine. It was found that immobilized beads are more efficient than the bulk form for pH recovery, sulfate and heavy metal removal.

Exploring Planting Strategies through Monitoring of a Greenspace Established in the Riparian Zone - The Case of an Implementation Site in Gapyeong County - (수변구역 조성 녹지의 모니터링을 통한 식재방안 모색 - 가평군 시공지를 대상으로 -)

  • Jo, Hyun-Kil;Park, Hye-Mi
    • Journal of Environmental Science International
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    • v.25 no.12
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    • pp.1689-1699
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    • 2016
  • The growth conditions of planted trees, invasion of nuisance herbaceous species, competition between species, and effects of erosion control were monitored over five years in a riparian greenspace in Gapyeong County that was established through multilayered and grouped ecological planting. Of 156 trees planted in the upper and middle layers, 5.8% died. This tree death was attributed to poor drainage or aeration in the rooting zone from the clay-added root ball and too deep planting as well as a small-sized root ball and scanty fine roots. Of all the trees, 21.6% grew poorly due to transplant stress in the first year after planting, but they started to grow vigorously in the third year. This good growth was largely associated with soil improvement before planting, selection of appropriate tree species based on growth ground, and control of dryness and invasive climbing plants through surface mulching and multilayered/grouped planting. Mixed planting of fast-growing species as temporary trees was desirable for accelerating planting effect and increasing planting density. Thinning of fast-growing trees was required in the fifth year after planting to avoid considerable competition with target species. To reduce the invasion of herbaceous and climbing plants that oppress normal growth of planted trees, higher density planting of trees (crown opening of about 15%), woodchip mulching to a 10-cm depth, and edge planting 2 m wide were more effective than lower density planting (crown opening of 70%), no surface mulching, and no edge planting, respectively. This reduction effect was especially great during the first three years after planting. Nuisance herbaceous plants rarely invaded higher density planting with woodchip mulching over the five years. Higher density planting or woodchip mulching also showed much greater erosion control through rainfall interception and buffering than lower density planting with no mulching did. Based on these results, desirable planting and management strategies are suggested to improve the functions of riparian greenspaces.

Control Effects of Benzylideneacetone Isolated from Xenorabdus nematophilla K1 on the Diseases of Redpepper Plants (Xenorhabdus nematophilla 유래물질 벤질리덴아세톤의 고추 병해 방제 효과)

  • Park, Su-Jin;Jun, Mi-Hyun;Chun, Won-Su;Seo, Ji-Ae;Yi, Young-Keun;Kim, Yong-Gyun
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.170-175
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    • 2010
  • A monoterpenoid benzylideneacetone (BZA) is a bacterial metabolite isolated from culture broth of an entomopathogenic bacterium, Xenorhabdus nematophila K1. It was tested in this study the control efficacy of the metabolite against two major fungal diseases occurring in red-pepper plants. BZA exhibited significant antifungal activities against Phytophthora capsici and Colletotrichum acutatum. Under natural light conditions, the antifungal activity of BZA was maintained for more than sixty days. The antifungal activity of BZA was not lost even in soil because the incidence of Phytophthora blight against red-pepper plants was significantly reduced when the suspensions of P. capsici were poured to the rhizosphere soils mixed with BZA. Application of the BZA suspension spray to the fruit surface infected with C. acutatum significantly suppressed the disease occurrence of anthracnose on the red-pepper plants. These results suggest that BZA can be used to develop a promising agrochemical to control phytophthora blight and anthracnose of redpepper plants.

Inhibitory Activity of Surfactants against Tobacco Mosaic Virus Infection (계면활성제의 TMV 감염저지 효과)

  • 박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.11 no.1
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    • pp.11-17
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    • 1989
  • Inhibition of tobacco mosaic virus (TMV) infection by 4 surfactants, sodium salts of alpha olefin (AOS), linear alkylbenzene (LAS), dioctyl sulfosuccinate (OSS), and dodecyl benzene sulfonic acid (SAS), was examined on tobacco cv. Xanthi-nc and NC 82. Infection of virions or TMV RNA was inhibited over 98% by the surfactants (2500 rpm). However, symptom development and viral concentration in tobacco plants treated with the surfactants into the rhizosphere soil 3 days before inoculation with TMV on leaves were not different from those in untreated tobacco plants. This indicates no significant systemic effects of the surfactants on the inhibition of TMV infection. The surfactants, except LAS, had no effect on the inhibition of viral infection when purified virions mixed with each surfactant and ultracentrifuged were inoculated on the tobacco plants. The virus was almost inactivated by LAS, showing that the viral infection was reduced more than 96%. The virus particles treated with the surfactants were not distinguishable in size and dimension from untreated normal particles, suggesting that the inhibitory action of the surfactants to TMV infection may not involve disintergration or uncoating of the virus at the early stage of infection.

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Micropropagation of Philodendron wend-imbe through Adventitious Multi-bud Cluster Formation (다아체 형성에 의한 Philodendron Wend-imbe의 대량번식)

  • Han, Bong-Hee;Yae, Byeoung-Woo;Goo, Dae-Hoe;Yu, Hee-Ju
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.115-119
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    • 2004
  • In order to micropropagate uniform plantlets of Philodendron wend-imbe, the shoot tips were cultured on media supplemented with 0.5-10.0mg/L BA or 0.01-1.0 mg/L thidiazuron (TDZ). The multi-bud clusters from basal part of shoots formed vigorously on media containing 5.0-10.0 mg/L BA or 0.05-0.1 mg/L TDZ. Shoot formation from the bud cluster sections (5-7mm) was achieved favorably on medium with 5.0mg/L BA and 20 g/L sucrose. Lowering of sucrose in medium to 20 g/L was effective for the inhibition of callus growth from basal part of shoots. Growth of shoots and their rooting were favorable on media containing 1.0-2.0 mg/L IBA or 0.1mg/L NAA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1: peat moss 1 or peat moss alone.

Effects of Growth Regulators and Sucrose Concentrations on the Bulblet Formation through In Vitro Culture of Scale Segment in Nerine bowdenii (네리네(Nerine bowdenii)의 기내 인편배양시 자구형성에 미치는 생장조절제와 Sucrose 농도의 영향)

  • Lee, Seung-Yeob;Ahn, Jeong-Ho;Park, Yun-Jum
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.139-143
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    • 2004
  • The twin-scale segments of nerine (Nerine bowdenii) were cultured to investigate the influence of NAA, BA and sucrose concentrations on in vitro bulblet formation. The formation of bulblets from twin-scale segments showed a good response both the percentage of bulblet formation and the number of bulblets per explant on MS medium supplemented with 1mg/L NAA and 2 mg/L BA. Formation of bulblet showed the highest efficiency on medium containing 30g/L, and the formation of bulblets was strongly inhibited on medium containing over 90g/L. When the twin-scale segments formed bulblets were subcultured three times to the same medium by 60 day subculture interval, the number of bulblets per explant was 6.5, 7.3 and 8.2 in order of first, second and third. The bulblets over 3mm in diameter were hypertrophied and rooted after transferring to the hormone-free MS medium. The plantlets over 50mm in height were successfully acclimatized in the soil mixed with the same volume of vermiculite and perlite, and the survival rate was over 95%.

Characteristics of the symbionts Pseudomonas sp. J2W strain and Xanthomonas sp. J2Y strain which utilize polyvinyl alcohol (Polyvinyl alcohol 이용 공생균 Pseudomonas sp. J2W와 Xanthomonas sp. J2Y의 특성)

  • Jo, Youn-Lae
    • Applied Biological Chemistry
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    • v.35 no.1
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    • pp.30-35
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    • 1992
  • Two strains J2W and J2Y which were isolated from soil can utilize polyvinyl alcohol(PVA) as a sole carbon source. PVA was utilized symbiotically by the mixed culture of these two strains which could not utilize PVA in each respective pure culture. Effect of degree of PVA polymerization on the its utilization was examed, and there was remarkable difference among three kind of PVA(PVA 500, 1500 and 2000). The reconstruction of there two strains was carried out with other symbionts Pseudomonas sp. PW and Pseudomonas sp. G5Y which were able to utilize PVA. PVA utilization occured in each remixed culture of J2Y strain with Pseudomonas sp. PW J2W strain with Pseudomonas sp. G5Y, respectively. Identification of bacteria was based on morphological and biological chatacteristics, J2W and J2Y strain were similar to a strain of Pseudomonas pseudimallei and Xanthomonas campestris, respectively.

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Growth and Mycorrhizal Formation of Pinus thunbergii Seedlings Grown in Growth Chamber (Growth Chamber 내(內)에서 생육(生育)한 해송묘(海松苗)의 생장(生長)과 균근형성(菌根形成))

  • Oh, Kwang In;Park, Whoa Shig
    • Journal of Korean Society of Forest Science
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    • v.79 no.3
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    • pp.316-321
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    • 1990
  • This study was carried out to indentify the mycorrhizal development and growth stimulation of Pinus thunbergii seedlings grown on soil growth media which mixed with various amounts of pine bark. The results were follow ; 1. Seedlings inoculated with Pisolithus tinctorius were significantly increased in number of short roots and mycorrhizal short roots, height, maximum growth rate, and leaf area than those of no-inoculation. 2. Mycorrhizal formation according to bark contents was highest by 25% bark treatment, and decreased in 50 and 75% bark contents. 3. Within the same treatment of bark content, seedlings inoculated with Pisolithus tinctorius showed more increased height and leaf, stem, and root dry weight than those of no-inoculated.

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