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http://dx.doi.org/10.5010/JPB.2004.31.2.115

Micropropagation of Philodendron wend-imbe through Adventitious Multi-bud Cluster Formation  

Han, Bong-Hee (원예연구소 원예생명공학과)
Yae, Byeoung-Woo (농촌진흥청 연구관리과)
Goo, Dae-Hoe (원예연구소 화훼과)
Yu, Hee-Ju (원예연구소 원예생명공학과)
Publication Information
Journal of Plant Biotechnology / v.31, no.2, 2004 , pp. 115-119 More about this Journal
Abstract
In order to micropropagate uniform plantlets of Philodendron wend-imbe, the shoot tips were cultured on media supplemented with 0.5-10.0mg/L BA or 0.01-1.0 mg/L thidiazuron (TDZ). The multi-bud clusters from basal part of shoots formed vigorously on media containing 5.0-10.0 mg/L BA or 0.05-0.1 mg/L TDZ. Shoot formation from the bud cluster sections (5-7mm) was achieved favorably on medium with 5.0mg/L BA and 20 g/L sucrose. Lowering of sucrose in medium to 20 g/L was effective for the inhibition of callus growth from basal part of shoots. Growth of shoots and their rooting were favorable on media containing 1.0-2.0 mg/L IBA or 0.1mg/L NAA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1: peat moss 1 or peat moss alone.
Keywords
BA; micropropagation; Philodendron; sucrose; thidiazuron;
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