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http://dx.doi.org/10.5010/JPB.2004.31.2.139

Effects of Growth Regulators and Sucrose Concentrations on the Bulblet Formation through In Vitro Culture of Scale Segment in Nerine bowdenii  

Lee, Seung-Yeob (원광대학교 식물자원과학부 생명자원과학연구소)
Ahn, Jeong-Ho (원광대학교 식물자원과학부 생명자원과학연구소)
Park, Yun-Jum (원광대학교 식물자원과학부 생명자원과학연구소)
Publication Information
Journal of Plant Biotechnology / v.31, no.2, 2004 , pp. 139-143 More about this Journal
Abstract
The twin-scale segments of nerine (Nerine bowdenii) were cultured to investigate the influence of NAA, BA and sucrose concentrations on in vitro bulblet formation. The formation of bulblets from twin-scale segments showed a good response both the percentage of bulblet formation and the number of bulblets per explant on MS medium supplemented with 1mg/L NAA and 2 mg/L BA. Formation of bulblet showed the highest efficiency on medium containing 30g/L, and the formation of bulblets was strongly inhibited on medium containing over 90g/L. When the twin-scale segments formed bulblets were subcultured three times to the same medium by 60 day subculture interval, the number of bulblets per explant was 6.5, 7.3 and 8.2 in order of first, second and third. The bulblets over 3mm in diameter were hypertrophied and rooted after transferring to the hormone-free MS medium. The plantlets over 50mm in height were successfully acclimatized in the soil mixed with the same volume of vermiculite and perlite, and the survival rate was over 95％.
Keywords
Bulblet formation; growth regulator; Nerine bowdenii; sucrose; twin scales;
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