• ALGAE
• /
• v.31 no.4
• /
• pp.391-401
• /
• 2016

To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

• Journal of Plant Biology
• /
• v.37 no.4
• /
• pp.403-410
• /
• 1994

The taxonomic criterion between Erythroglossum and Sorella was reappraised based on Korean plants of E. minimum Okamura and S. repens (Okamura) Hollenberg. Development of the reproductive organs was observed from field collected plants as well as cultured plants. The Polyneura-tuype procarp which comprised one group of sterile cells and two carpogonial branches arising from a supporting cell was observed in both species. Distribution of tetrasporangial sori was irregular in both species, so it is not relevant to distinguish Sorella from Erythroglossum by the characteristic of tetrasporangial distribution. E. minimum and S. repens were very similar in vegetative form, but were reproductively isolated. Chromosome numbers were given as n=ca. 30 in S. repens and n=ca. 41 in E. minimum, respectively.

• Journal of Korea Spatial Information System Society
• /
• v.6 no.2 s.12
• /
• pp.23-37
• /
• 2004

Location Based Services(LBSs) in mobile environments become very popular recently. For efficient LBSs, spatial database management systems must need a spatial indexing scheme such as R-trees in order to manage the huge spatial database. However, it may need unnecessary disk accesses since it needs to access objects which are not actually concerned to user's location-based queries. In this paper, to support the location-based queries efficiently, we propose a CLR-tree(Cell Leveling R-tree) in which a dynamic cell is built up within the minimum bounding rectangle of R-trees' node. The cell level of nodes is compared with the query's cell level in location-based query processing and determines the minimum search space. Also, we propose the insertion, split, deletion, and search algorithms for CRL-trees. From the experimental results, we see that a CLR-tree is able to decrease $5{\sim}20%$ of disk accesses from those of R-trees. So, a CLR-tree can be used for fast accessing spatial objects to user's location-based queries in LBSs.

• Transactions of the Korean hydrogen and new energy society
• /
• v.14 no.3
• /
• pp.236-246
• /
• 2003

This paper focuses on developing a model of a PEM fuel cell stack and to integrate it with realistic model of the air supply system for fuel cell vehicle application. The fuel cell system model is realistically and accurately simulated air supply operation and its effect on the system power and efficiency using simulation tool Matlab/Simulink. The Peak performance found at a pressure ratio of 3, and it give a 15mV increase per cell. The limit imposed is a minimum SR(Stoichiometric Ratio) of 2 at low fuel cell load and 2.5 at high fuel cell load.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.7 no.2
• /
• pp.207-216
• /
• 1995

Flow and heat transfer characteristics of mixed convection heat transfer in a rectangular en-closure with various outlets are numerically investigated. The parameters considered here include Reynolds number, Grashof number and the position of outlet. The results show streamlines, isotherms, Nusselt numbers, velocity and temperature distributions. It has been shown that as Reynolds number increases, the size of cell decreases at Re$\leq$100 and increases at Re>100 for $Gr=10^4$. There is a minimum size of cells at Re=100, $Gr=10^4$. The maximum mean Nusselt number occurs at Re=400, $Gr=10^4$ and one right outlet. The mean Nusselt numbers can be formulated by the correlation equation $Nu=C{\cdot}Gr^a{\cdot}Re^b$.

• Journal of Ocean Engineering and Technology
• /
• v.10 no.4
• /
• pp.109-117
• /
• 1996

The numerical and experimental studies on the mixed convection of a rectangular enclosure for indoor air control has been studied numerically using the finite volume method and experimentally with holographic interferometry and Laser apparatus. The parameters studied here are Pr= 0.71, $30{\leq}Re{\leq}600$, $700{\leq}Gr{\leq}100000$ and the positions of an outlet. The results show streamlines, isothrems, velocity distributions and photograph of holographic interferometry and streamlines. It is found that the minimum cell occurs at Re=100, Gr=10000. The mean Nusselt numbers can be expressed by the correlation equation $Nu=C{\cdot}Gr^a$ $Re^b.$ The comparison of numerical and experimental results shows a good agreement.

• Proceedings of the KIEE Conference
• /
• 2008.07a
• /
• pp.1110-1111
• /
• 2008

The life time of PV module is semi-permanent. But, because of installation and module fabrication process, its important part can not be finished. In this paper, we analyze 15 years old modules made from different company. Among the PV modules, the maximum power drop ratio was 12.23% minimum and 80.63% maximum. Also the effect of solar cell's short circuit current difference was analyzed. The PV module exposed about 65days, its the maximum power drop ratio was 1.29% minimum and 23.43% maximum. It is for reduction of current value. And the reason for current reduction was due to reduction of parallel resistance of solar cell. To prevent early degradation, it is need to have attention to fabrication, installation and maintenance.

• ALGAE
• /
• v.20 no.3
• /
• pp.233-238
• /
• 2005

The photosynthetic parameters of dark- adapted minimum fluorescence (Fo) and maximum quantum yield of charge separation in PSII (Fv/Fm) were measured in transverse sections of eight species of marine Phaeophyceae (species of Laminariales, Fucales, Desmarestiales, Chordariales) using pulse amplified modulation (PAM) fluorometry. Within each transverse section fluorescence was measured in three regions corresponding to outer cortical and meristoderm cells, inner cortical cells and innermost medullary cells. Minimum fluorescence declined from 19-74% (mean of 39%) from outermost to innermost cells. Maximum quantum yield varied from 0.51-0.59 in outermost cell layers and this was reduced to 0.23-0.40 in innermost cell layers, with an average reduction of 50%. Despite the reduction Fo in medullary cells (inner), medullas of all species showed maximum quantum yields consistent with a photosynthetic role in carbon fixation. These results show that medullary cells of complex brown algae have more than a role in structure, storage or transport, and may also provide an important role in carbon fixation.

• Mycobiology
• /
• v.30 no.2
• /
• pp.93-95
• /
• 2002

The antifungal activities of propolis on Cryptococcus neoformans and Candida albicans were evaluated. In microbroth culture assay, the MIC(minimum inhibitory concentration) of propolis for C. neoformans and C. albicans were 2 and 16 mg/ml, respectively. In propolis-included solid medium assay, the MIC of propolis for C. neoformans and C. albicans were 4 and 16 mg/ml, respectively. Propolis showed fungicidal activity against C. neoformans, whereas propolis possesed fungistatic activity against C. albicans. The MFC(minimum fungicidal concentration) for C. neoformans was 8 mg/ml. Cell morphology of C. neoformans was affected by treatment of propolis. In scanning electron microscope, the appearance of cell rupture was observed.

• The Journal of Korea Robotics Society
• /
• v.1 no.1
• /
• pp.9-16
• /
• 2006

The Mobile robots are increasingly being used to perform tasks in unknown environments. The potential of robots to undertake such tasks lies in their ability to intelligently and efficiently search in an environment. An algorithm has been developed for robots which explore the environment to measure the physical properties (dust in this paper). While the robot is moving, it measures the amount of dust and registers the value in the corresponding grid cell. The robot moves from local maximum to local minimum, then to another local maximum, and repeats. To reach the local maximum or minimum, simple gradient following is used. Robust estimation of the gradient using perturbation/correlation, which is very effective when analytical solution is not available, is described. By introducing the probability of each grid cell, and considering the probability distribution, the robot doesn't have to visit all the grid cells in the environment still providing fast and efficient sensing. The extended algorithm to coordinate multiple robots is presented with simulation results.