• Journal of Veterinary Science
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• 제22권6호
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• pp.41.1-41.14
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• 2021

Background: Our previously prepared ceftiofur (CEF) hydrochloride oily suspension shows potential wide applications for controlling swine Streptococcus suis infections, while the irrational dose has not been formulated. Objectives: The rational dose regimens of CEF oily suspension against S. suis were systematically studied using a pharmacokinetic-pharmacodynamic model method. Methods: The healthy and infected pigs were intramuscularly administered CEF hydrochloride oily suspension at a single dose of 5 mg/kg, and then the plasma and pulmonary epithelial lining fluid (PELF) were collected at different times. The minimum inhibitory concentration (MIC), minimal bactericidal concentration, mutant prevention concentration (MPC), post-antibiotic effect (PAE), and time-killing curves were determined. Subsequently, the area under the curve by the MIC (AUC_0-24h/MIC) values of desfuroylceftiofur (DFC) in the PELF was obtained by integrating in vivo pharmacokinetic data of the infected pigs and ex vivo pharmacodynamic data using the sigmoid E_max (Hill) equation. The dose was calculated based on the AUC_0-24h/MIC values for bacteriostatic action, bactericidal action, and bacterial elimination. Results: The peak concentration, the area under the concentration-time curve, and the time to peak for PELF's DFC were 24.76 ± 0.92 ㎍/mL, 811.99 ± 54.70 ㎍·h/mL, and 8.00 h in healthy pigs, and 33.04 ± 0.99 ㎍/mL, 735.85 ± 26.20 ㎍·h/mL, and 8.00 h in infected pigs, respectively. The MIC of PELF's DFC against S. suis strain was 0.25 ㎍/mL. There was strong concentration-dependent activity as determined by MPC, PAE, and the time-killing curves. The AUC_0-24h/MIC values of PELF's DFC for bacteriostatic activity, bactericidal activity, and virtual eradication of bacteria were 6.54 h, 9.69 h, and 11.49 h, respectively. Thus, a dosage regimen of 1.94 mg/kg every 72 h could be sufficient to reach bactericidal activity. Conclusions: A rational dosage regimen was recommended, and it could assist in increasing the treatment effectiveness of CEF hydrochloride oily suspension against S. Suis infections.

• The Korean Journal of Pain
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• 제18권2호
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• pp.118-123
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• 2005

Background: A nerve ligation injury may produce a tactile allodynia. The effects of intrathecally delivered lamotrigine on allodynia induced due to fifth and sixth lumbar spinal nerves ligation in rats, using lumbar intrathecal catheters were examined. Methods: Sprague-Dawley rats (body weight 160-180 g) were prepared by tightly ligating the fifth and sixth left lumbar spinal nerves, with the implantation of a chronic intrathecal catheter for drug administration. Mechanical allodynia and allodynic threshold were measured using von Frey filaments and the updown method, respectively. After the baseline hind paw withdrawal thresholds had been obtained, lamotrigine (10, 30, 100 and $300{\mu}g$) was administered intrathecally. Thereafter, the dose-response curves and 50% effective dose ($ED_{50}$) were obtained. Motor dysfunction was assessed by observing the righting/stepping reflex responses and abnormal weight bearing. Results: Intrathecal administration of lamotrigine produced a dose-dependent antiallodynic action ($ED_{50}=61.7{\mu}g$). Mild motor weakness was observed with $300{\mu}g$ lamotrigine, but no severe motor impairment was found. Conclusions: It is suggested that intrathecal lamotrigine could produce moderate antagonism of mechanical allodynia at the spinal level in a rat neuropathic pain model with minimal motor weakness.

• 치위생과학회지
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• 제11권1호
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• pp.37-40
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• 2011

Triterpenoid saponins 유도체인 OA와 UA가 치아우식증 원인균종의 하나인 Streptococcus downei의 표준균주(ATCC $33748^T$) 및 임상균주(4주)에 대하여, 액체배지희석법을 이용한 MIC값 측정 및 time-kill 분석법을 통한 살균작용 유무를 측정하여 다음과 같은 결과를 얻었다. 1. S. downei ATCC $33748^T$ 및 S. downei 임상균주(4주)들의 OA 대한 MIC 값은 $8{\mu}g/ml$로 동일하였고, UA의 대한 MIC 값은 S. downei 표준균주와 임상균주 간에 차이가 있었다. 2. Time-kill 분석법을 실시한 결과 OA와 UA는 S. downei $33748^T$에 대하여 $2{\times}MIC$ (각각 $16{\mu}g/ml$과 $8{\mu}g/ml$) 이상의 농도에서 살균작용을 갖는다는 것을 알 수 있었다. 이상의 연구결과를 종합할 때, triterpenoid saponins 유도체인 OA와 UA는 뮤탄스 연쇄상구균 중 최근에 사람의 구강 내 치면세균막에 서식하는 것으로 밝혀진 S. downei,에 대해 살균제로 작용할 수 있음을 알 수 있었다.

• 한국식품조리과학회지
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• 제31권5호
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• pp.515-523
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• 2015

In the study reported here, the antimicrobial and antioxidant activities of the ethanol leaf extract of Dendropanax morbiferus Lev. from Jeju Island was investigated. Of the 14 strains of 12 species of microorganisms tested, the extract exhibited antimicrobial activity observed against seven Gram-positive bacteria of four species, but not against six Gram-negative bacteria and a yeast strain. Using the disc diffusion method, the diameter of the inhibition zone increased with application of the extract with every strain and the highest growth inhibition was exhibited with Staphylococcus aureus KCTC 1916 at 5 mg/ml. The minimal inhibitory concentration of the extract (MIC) by turbidity was 2.5 mg/ml against Bacillus cereus KACC 12672 and 15 mg/ml when with Enterococcus faecalis KCTC 3206. The minimum bacterial concentration (MBC) values defined as being $${\geq_-}99.9%$$ reduction in viable cells against the tested strains was higher than the MIC values. Time killing curves using the optimum MIC were performed on seven strains incubated for 48 hr. The growth of B. cereus KACC 12672 was detected after 12 hr and no significant growth was found in the others strains after 48 hr (p<0.05). The 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity of the ethanol leaf extract was similar to that of butylated hydroxyanisole (BHA) at the same concentration. These results indicate that leaf extract of D. morbiferus Lev. can be utilized as a natural preservative and an antioxidant.

• International Journal of Oral Biology
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• 제35권4호
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• pp.191-195
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• 2010

Oleanolic acid is a natural triterpenoid that exists widely in foods and some medicinal herbs. The purpose of this study was to determine the antimicrobial activity of oleanolic acid against Streptococcus mutans strains isolated from a Korean population. Antimicrobial activity against these bacteria was evaluated by minimal inhibitory concentration (MIC) and time kill curves. The tolerance of human gingival fibroblasts and human periodontal ligaments to oleanolic acid was tested using a methyl thiazolyl tetrazolium (MTT) assay. The $MIC_{90}$ value of oleanolic acid for both S. mutans and S. sobrinus isolated from Koreans was 8 ${\mu}g/ml$. Oleanolic acid showed bactericidal effects against S. mutans ATCC $25175^T$ and S. sobrinus ATCC $33478^T$ at $1\;{\times}\;MIC$ ($8{\mu}g/ml$) and had no cytotoxic effects against KB cells at this dose. The results suggest that oleanolic acid could be useful in the future development of oral hygiene products for the prevention of dental caries.

• 한국지반공학회논문집
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• 제29권11호
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• pp.107-118
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• 2013

보강토 옹벽의 적용사례가 날로 증가하고, 일반화됨에 따라 그에 따른 피해도 종종 발생하고 있다. 최근 들어 게릴라성 집중강우 등으로 인해 보강토 옹벽이 붕괴되는 사례가 종종 발생되면서 붕괴된 옹벽의 복구방법에 대한 관심이 커지고 있다. 이와 같은 배경 하에 이 논문에서는 (1) 피해상태로 쏘일네일링을 사용하여 복구하는 안과 (2) 붕괴된 보강토 옹벽에 대해 보강토체 제거 후 재시공하는 안에 대해 한계평형해석과 수치해석을 수행하여 안정성과 거동을 분석하여 설계하고 시공한 사례를 소개하였다. 검토결과, 쏘일네일링 복구 옹벽의 경우 보강콘크리트 전면벽체에서 수평변위와 전단변형률 등은 거의 발생하지 않았으나, 재시공 옹벽은 수평변위의 경우 벽체 중앙부에서 발달하고 전단변형률은 옹벽의 저부를 중심으로 나선 형태로 발달하는 것으로 나타났다. 따라서, 피해옹벽은 쏘일네일링으로 복구하였으며, 일련의 시공과정을 소개하였다.

• 한국안전학회지
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• 제25권6호
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• pp.115-122
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• 2010

최근 들어 교량과 같은 구조물에 대한 생애주기비용(Life Cycle Cost, LCC) 분석의 중요성이 점차 커지고 있다. 그러나 교량의 공용수명 동안 발생할 수 있는 생애주기비용은 설계 및 시공조건 그리고 사용환경에 따라 많은 불확실성을 내포하고 있기 때문에 정확히 예측하기 힘들다. 본 논문에서는 교량의 생애주기비용 최적설계를 위한 설계방법을 제시하였다. 교량의 총생애주기비용은 초기비용, 손상비용 유지관리비용, 보수/보강비용, 사용자비용, 해제/폐기비용의 합으로 산정하였다. 생애주기비용을 목적함수로 하고 도로교설계기준을 제약조건으로 최적화를 수행하였다. 초기비용은 종합불가정보 및 참고자료를 근거로 산출하였으며, 하중 및 부재에 대한 불확실성을 고려하기 위해 손상확률의 개념을 도입하여 손상비용을 산출하였다. 교량의 내하율곡선을 이용하여 교량의 보수/보강 비용을 추정하였으며, 차량운행비용과 시간지연비용으로부터 사용자비용을 산정하였다. 이로부터 교량에 대한 생애주기비용 최적설계를 수행하고 주요인자들에 대한 영향을 살펴보았다.

• Animal Bioscience
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• 제34권6호
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• pp.949-956
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• 2021

Objective: This study was focused on the estimation of parameters of Wood's model and description of the lactation curve using the cows which were lactated over 24 months on the first lactation. Methods: The database included 1,333 pure-bred dairy Simmental primiparous cows which lactated for 24 months (732 days). The initial dataset entering the procedure of assessment of parameters of Wood's function included 35,826 milk yield records. Milk yield was recorded throughout lactation, with the earliest record taken on day 6 and the latest on day 1,348 of lactation. This dataset was used for the assessment of parameters a, b, c of Wood's model using the non-linear statistical procedure. These parameters were estimated for different length of lactation. The assessed parameters were used for calculation of some characteristics of lactation curves. Results: The lowest value of a parameter (15.2317) of Wood's model of lactation curve was found out in lactations up to 305 days long, contrary to b and c parameters which were highest in those lactations (0.1029 and 0.0015, respectively). The maximum value of a parameter (17.4329) was found out in lactations up to 640 days long, unlike b and c parameters which were minimal in those lactations (0.0603 and 0.0010, respectively). Conclusion: It can be concluded that the parameters of Wood's model and the shape of lactation curve are changing with the growing number of milk yield records. Also, the assessed parameters revealed a significant milk production potential after 305 days of lactation.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권7호
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• pp.2610-2630
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• 2021

The aim of this paper is to propose the alternative algorithm to finish the process in public key cryptography. In general, the proposed method can be selected to finish both of modular exponentiation and point multiplication. Although this method is not the best method in all cases, it may be the most efficient method when the condition responds well to this approach. Assuming that the binary system of the exponent or the multiplier is considered and it is divided into groups, the binary system is in excellent condition when the number of groups is small. Each group is generated from a number of 0 that is adjacent to each other. The main idea behind the proposed method is to convert the exponent or the multiplier as the subtraction between two integers. For these integers, it is impossible that the bit which is equal to 1 will be assigned in the same position. The experiment is split into two sections. The first section is an experiment to examine the modular exponentiation. The results demonstrate that the cost of completing the modular multiplication is decreased if the number of groups is very small. In tables 7 - 9, four modular multiplications are required when there is one group, although number of bits which are equal to 0 in each table is different. The second component is the experiment to examine the point multiplication process in Elliptic Curves Cryptography. The findings demonstrate that if the number of groups is small, the costs to compute point additions are low. In tables 10 - 12, assigning one group is appeared, number of point addition is one when the multiplier of a point is an even number. However, three-point additions are required when the multiplier is an odd number. As a result, the proposed method is an alternative way that should be used when the number of groups is minimal in order to save the costs.

• Journal of Veterinary Science
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• 제23권5호
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• pp.78.1-78.13
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• 2022

Background: Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis. Objectives: In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites. Methods: The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied. Results: The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively. The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively. Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks. The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline. The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 ㎍/mL, respectively. The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent. Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis. Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver. Conclusions: Florfenicol composite nanogels might improve the treatment of SCV infections.