International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Antimicrobial Effects of Oleanolic Acid against Streptococcus mutans and Streptococcus sobrinus Isolated from a Korean Population

  • Kim, Min-Jung (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Kim, Chun-Sung (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Ha, Woo-Hyung (Department of Dental Materials, School of Dentistry, Chosun University) ;
  • Kim, Byung-Hoon (Department of Dental Materials, School of Dentistry, Chosun University) ;
  • Lim, Yun-Kyong (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Park, Soon-Nang (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Cho, Yu-Jin (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Kim, Myung-Mi (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Ko, Jang-Hyuk (Department of Dental Materials, School of Dentistry, Chosun University) ;
  • Kwon, Soon-Sung (Department of Dental Materials, School of Dentistry, Chosun University) ;
  • Ko, Yeong-Mu (Department of Dental Materials, School of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • Received : 2010.11.03
  • Accepted : 2010.12.16
  • Published : 2010.12.31
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Abstract

Oleanolic acid is a natural triterpenoid that exists widely in foods and some medicinal herbs. The purpose of this study was to determine the antimicrobial activity of oleanolic acid against Streptococcus mutans strains isolated from a Korean population. Antimicrobial activity against these bacteria was evaluated by minimal inhibitory concentration (MIC) and time kill curves. The tolerance of human gingival fibroblasts and human periodontal ligaments to oleanolic acid was tested using a methyl thiazolyl tetrazolium (MTT) assay. The $MIC_{90}$ value of oleanolic acid for both S. mutans and S. sobrinus isolated from Koreans was 8 ${\mu}g/ml$. Oleanolic acid showed bactericidal effects against S. mutans ATCC $25175^T$ and S. sobrinus ATCC $33478^T$ at $1\;{\times}\;MIC$ ($8{\mu}g/ml$) and had no cytotoxic effects against KB cells at this dose. The results suggest that oleanolic acid could be useful in the future development of oral hygiene products for the prevention of dental caries.

Keywords

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