• 생약학회지
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• 제35권1호통권136호
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• pp.41-44
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• 2004

An Chung Hwan(安中丸) is a Chinesε patent medicine, which has been used for various purposes in Korea. An Chung Hwan is composed of 15 kinds of powdered crude drugs and has been used for stomachic disorder, acute and chronic indigestion, dyspepsia, and diarrhea. For the identification of individual crude drugs in such powdery mixtures, microscopic method may advantageously be used as it requires only a small amount of specimens. In this study, it is demonstrated that the microscopic method is very effective for the identification of 15 crude drug ingredients in An Chung Hwan.

• The Journal of Korean Physical Therapy
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• 제3권1호
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• pp.229-250
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• 1991

The study was carried out to investigate and review the principles and practices of immunocytochemical method in light microscopic level. The results were as follows. 1. Immunocytochemistry is the method to search out the intracellular position of the specific meterials using antigen -antibody reaction. 2. The chief items in immunocytochemistry are antigen, antibody and chromogen. 3. The identifical fixation is cardiac perfusion fixation. 4. The tissue slides must be prepared by vibratomy. 5. All stainings are carried out with free floating staining method. 6. There are polyclonal and monoclonal antibodies used in immunocytochemistry.

• Geomechanics and Engineering
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• 제20권6호
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• pp.517-525
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• 2020

The long-term stability of rock engineering is significantly affected by the time-dependent deformation behavior of rock, which is an important mechanical property of rock for engineering design. Although the hard rocks show small creep deformation, it cannot be ignored under high-stress condition during deep excavation. The inner mechanism of creep is complicated, therefore, it is necessary to investigate the relationship between microscopic creep mechanism and the macro creep behavior of rock. Microscopic numerical modeling of sandstone creep was performed in the investigation. A numerical sandstone sample was generated and Parallel Bond contact and Burger's contact model were assigned to the contacts between particles in DEM simulation. Sensitivity analysis of the microscopic creep parameters was conducted to explore how microscopic parameters affect the macroscopic creep deformation. The results show that the microscopic creep parameters have linear correlations with the corresponding macroscopic creep parameters, whereas the friction coefficient shows power function with peak strength and Young's modulus, respectively. Moreover, the microscopic parameters were calibrated. The creep modeling curve is in good agreement with the verification test result. Finally, the creep curves under one-step loading and multi-step loading were compared. This investigation can act as a helpful reference for modeling rock creep behavior from a microscopic mechanism perspective.

• 한국기계가공학회지
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• 제10권3호
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• pp.100-107
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• 2011

To evaluate the wear characteristics of very thin ceramic coated layer, it is very important to investigate its wear process in great detail. An effective method for investigating the wear of a thin layer is the observation of wear process in microscopic detail, using in-situ system. In this study, based on the SEM Tribosystem as in-situ system, the microscopic wear mode of TiN coatings was investigated in repeated sliding. Consequently, four modes were revealed for TiN coatings: Ploughing, powder formation, flake formation and coating delimitation. Sc(Severity of contact) can clarify transition condition of those microscopic wear modes.

• 한국정밀공학회지
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• 제14권9호
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• pp.146-154
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• 1997

Ion implantation has been used successfully as a surface treatment technology to improve the wear. fatigue and corrosion resistances of materials. A modified surface layer by ion implantation is very thin(under 1 m), but it has different mechanical properties from the substrate. It has also different wear characteristics. Since wear is a dynamic phenomenon on interacting surfaces with relative motion, an effective method for investigtating the wear of a thin layer is the observation of wear process in microscopic detail using in-situ system. The change of wear properties produces the transition of wear mode. To know the microscopic wear mechanism of this thin layer, it is very important to clarify its microscopic wear mode. In this paper, using the SEM and AFM Rribosystems as in-situ system, the microscopic wear of Ti ion-implanted 1C-3Cr steel, a material for roller in the cold working process, was investigated in repeated sliding. The depth of wear groove and the speciffc wear amount were changed with transition of microscopic wear mode. The depth of wear groove with friction cycles in AFM tribosystem and specific wear amount of Ti ion-implanted 1C-3Cr steel were less about 2-3 times than those of non-implanted 1C-3Cr steel. The microscopic wear mechansim of Ti ion-implanted 1C-3Cr steel was also clarified. The microscopic wear property was quantitatively evaluated in terms of microscopic wear mode and specific wear amount.

• Applied Microscopy
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• 제42권1호
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• pp.49-52
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• 2012

For TEM study of biological samples or polymers that are contained in organic structure, it is often required that the sample is prepared by using ultramicrotome and stained with proper agents to increase the contrast of organic structure. In this study, we investigated an efficient TEM sample preparation method for ductile heterogeneity material by using ultramicrotomy. Cryo-ultramicrotomy is a suitable method that is capable of rendering sample hardness for various ductile materials. However, it has several factors to consider, such as experimental cost, working time and finding the optimal staining conditions. To satisfy these considerations, we prepared TEM sample by using ultramicrotome without cryofunction, and secured the sample hardness by applying the staining process prior to ultrathin sectioning. The cross-linked polyethylene structure in the sample was stained with the 2% $RuO_4$ solution in a sealed test tube for 24 hours at $4^{\circ}C$. After the sample staining, ultrathin sections of sample were prepared using ultramicrotome. As a result, it was revealed that the difficulties associated with staining of ultrathin sections prepared by low-temperature conditions were improved. In addition, appropriate staining depth of sample could be selected for sectioning process. The quality of TEM sample obtained by using this method was better than that of cryo-ultramicroscopy. Finally, it is expected that our method could be effectively applied in TEM sample preparation for a variety of nano-bio convergence materials.

• 한국가금학회지
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• 제50권4호
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• pp.267-272
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• 2023

본 연구에서는 닭진드기에 감염된 산란계 농장을 대상으로 3point 5score 현미경 모니터링 방법을 이용하여 방제 골든타임(golden time)과 충란제거제를 결합한 닭진드기 종합방제법(Integrated Pest Management; IPM)의 효과를 조사하기 위해 수행되었다. 처리구는 닭진드기에 감염된 총 8개 산란계 농장을 바탕으로 1) 닭진드기 종합방제법을 이용한 농가 그리고 2) 일반적인 닭진드기 방제법을 이용한 농가로 2가지 처리구로 구분하였다. 또한, 농가의 닭진드기 오염도는 3point 5score 현미경 모니터링 방법을 이용하여 평가하였다. 그 결과, 산란계 농장에 대한 IPM 적용의 유무에 따른 청소 및 소독 전, 후 그리고 4주부터 20주까지의 score는 유의적인 차이를 보이지 않았다(P>0.05). 하지만, 24주에는 IPM을 적용한 농가가 IPM을 적용하지 않은 농가보다 유의적으로 낮은(P<0.05) Score를 보였다. 따라서 본 연구의 결과를 바탕으로, 3point 5score 현미경 모니터링 방법으로 지속적인 모니터링과 보완과 함께 충란제거제를 포함한 IPM을 이용하여 닭진드기를 제어하는 것은 궁극적으로 목표로 하는 닭진드기의 감염률을 감소시키는 데 효과적인 것으로 판단된다.

• 한국방재학회 논문집
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• 제8권1호
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• pp.31-37
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• 2008

기존의 교통영향평가 지침으로 규정된 사업 시행 전후의 교통흐름은 가로구간의 경우 교통량, V/C, 평균통행속도와 서비스수준, 교차로의 경우 서비스 수준 등의 교통류 분석지표에 의하여 분석되고 있다. 이러한 교통류 분석지표는 확률론적(stochastic)인 교통류의 특성을 효율적으로 번영하지 못하며, 특히 개별 가로구간과 교차로에 국한하여 분석됨으로서 사업 시행으로 인한 교통영향 분석 범위 전체 구간의 교통흐름을 종합적으로 분석하기에는 미흡하다. 본 연구는 차량추종모형(Car-Following Theory)에 의한 미시적 시뮬레이션(microscopic-simulation) 기법을 적용하여, 개별 구간과 교차로 분석 범위를 넘어서 사업 대상 교통영향 반경 내의 교통흐름을 가시적(visual)하게 보여줄 수 있는 방안과 이를 바탕으로 공간적 범위 설정 개선방안, 개선대책 수립에 따른 효과분석 방안 등 다양한 분석결과를 도출하였다.

• 콘크리트학회지
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• 제10권6호
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• pp.203-211
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• 1998

고체내부의 미소파괴시 발생하는 탄성파 방출을 이용하는 음향방출기법은 구조물 또는 재료 내부의 미시적 변형기구를 이해하는데 매우 유익한 수단으로 최근 각 분야에서 다양하게 응용되고 있다. 따라서 본 연구에서는 모르타르 부재의 휨재하 시험시 부재 내부에 발생하는 미시적 손상거동 및 파괴특성을 시험시 연속적으로 모니터링한 AE 신호특성으로부터 평가하였다. 나아가 삼각법을 이용한 2차원 AE 발생원 위치추정으로부터 시험체 노치선단 주변에 대한 AE 발생원 위치를 명확히 하였으며 이들 결과로부터 미소균열의 성장 거동을 연속적으로 모니터링 하였다.

• 한국토양비료학회지
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• 제6권4호
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• pp.253-255
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• 1973

Microscopic method was compared with X-ray diflraction analysis for the identification of rockforming minerals using 11 main rock samples in Korea. 1. There was no difference between X-ray diffraction analysis and microscopic one in major minerals, but some accessary minerals. 2. The rock-forming minerals of main rocks presented in this study occured almost in crystalline state so that they could be easily identified by X-ray analysis alone.