• Journal of Environmental Science International
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• v.28 no.1
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• pp.159-162
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• 2019

This study aimed to evaluate the effects of dietary microbial-fermented molasses on egg production and egg quality in laying hens.In total, 90 Hy-line Brown laying hens were divided into two treatment groups (control and 1% microbial-fermented molasses)with three replicates of 15 birds each. During the experimental period, supplementation of hen diets with 1% microbial-fermented molassesdid not influence egg weight, hen-day egg production, egg mass, and feed conversion ratio (p > 0.05), except for feed intake. Regarding egg quality, diets containing 1% microbial-fermented molasses significantly affected eggshell thickness, Haugh unit, and albumen height (p < 0.05). However, there were no remarkable differences between control and 1% microbial-fermented molasses in eggshell color and egg yolk color (p > 0.05). These results indicate that supplementing 1% microbial-fermented molasses to the diet of laying hens improved egg quality parameters such as eggshell thickness, Haugh unit, and albumen height rather than egg production.

• Journal of the Korean Society of Clothing and Textiles
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• v.42 no.3
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• pp.516-529
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• 2018

This study investigated the dyeing properties and bio-functions of cotton fabrics dyed with naturally fermented Ecklonia cava extract in order to compare it with a comparison of unfermented extract. Hot water-extracted Ecklonia cava was fermented naturally under the various conditions of a fermenting period (2-8 days) and amount of molasses (0.1-1.8% v/v); in addition, it was also tested for characterization by FT-IR, antioxidant activity, total polyphenol content, and anti-microbial activity. For dyed cotton fabrics, color strength (K/S), physical color properties, dyeing fastness, sun protective property, and anti-microbial activity were evaluated considering dyeing conditions. As a result, the fermented dye under fermentation condition of 0.1% v/v with molasses during 4 days was revealed as having a similar chemical structure to the unfermented one and showed a total polyphenol content with 32.88mg/g and better antioxidant activity than the unfermented one. As for dyed fabrics, the color strength value by K/S was the highest under the condition of 0.1% v/v of molasses during 4 days among all fermenting conditions. The dyed fabrics had a reasonably good fastness (except for light). Anti-microbial activity against K. pneumoniae was better for the fermented extract-dyed fabric especially with lower dye concentrations.

• Journal of Life Science
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• v.26 no.5
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• pp.571-583
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• 2016

Biogenic amines are produced primarily by microorganisms found in fermented foods and are often implicated in poisoning incidents in humans. In this study, 620 strains of microorganisms were isolated from traditional Korean fermented food in Sunchang in order to screen for non-biogenicamine-producing microorganisms present in these foods. One strain was identified and named Bacillus subtilis SCJ1, by using 16S rRNA sequencing and biochemical characterization. We investigated the cell growth of this organism in order to understand its potential for industrial application. To this end, we optimized the culture medium constituents by using the response surface methodology. The Plackett-Burman experimental design was used for screening of the medium constituents, such as molasses, yeast extract and peptone, for improving cell growth. In order to determine the optimal concentration of each constituent, we used a central composite design. Consequently, the optimized concentrations of molasses, yeast extract and peptone were predicted to be 27.5 g/l, 7.5 g/l and 17.5 g/l, respectively. By model verification, we confirmed that a 1.49-fold increase in dry cell weight compared to the basal medium-from 1.32 g/l, to 1.9722 g/l-was achieved.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.35-46
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• 1997

An experiment with growing bulls were conducted to determine the effect of supplementation of a straw (S) with 15% molasses and 3% urea as an intimate mix (UMS) on its dry matter (DM) intake and digestibility (DMD) and reduction of methane ($CH_4$) production from fermentation in vitro of the straw. In the next experiment, the feeding of the UMS was compared with that of the feeding of molasses and urea in meals (DS) or in lick blocks (DSUMB) as supplements to straw. The UMS feeding increased daily intake of straw DM ($89.5 g{\cdot}kgW^{-0.75}$, p < 0.01) and digestible crude protein (DCP 333 g, p < 0.001) and nitrogen (N) balances ($508mg{\cdot}kgW^{-0.75}{\cdot}d^{-1}$, p < 0.01) of the bulls than the feeding of 'S' ($65g{\cdot}kgW^{-0.75}$, 55 g and $8.0mg{\cdot}kgW^{-0.75}{\cdot}d^{-1}$, respectively). It also increased the digestibility of DM ($594g{\cdot}kg^{-1}$, p < 0.05), organic matter (OM, $641g{\cdot}kg^{-1}$, p < 0.05), CP ($619g{\cdot}kg^{-1}$, p < 0.001) and acid detergent fibre (ADF, 773, p < 0.05). The $CH_4$ emitted per g of DOM apparently fermented in the rumen (DOMR) was 91.0 ml in the 'S' and reduced (p < 0.05) to 61.6 ml in the UMS. The feeding of the UMS when compared with that of the DS or DSUMB also gave a higher straw intake (1.77% of live weight, LW, p <0.01) and ADF digestibility ($516g{\cdot}kg^{-1}$, p < 0.05) than the other diets (1.52% or 1.55% LW and 472 or $490g{\cdot}kg^{-1}$, respectively) in association with the increased microbial N yield in the rumen (14.1, 5.62 or $17.0g{\cdot}kg^{-1}$ DOMR, respectively, p < 0.05), daily LW gains (233, 125 or 93 g, respectively, p < 0.05) and feed conversion ratios of the diets (26.0, 56.1, or 57.6 g feed/g LW gain, p > 0.05, respectively). It can be concluded that molasses and urea feeding as an intimate mix with straw (UMS) increased its digestion and intake in association with a reduced methane emissions in the rumen. When compared with that of their feeding in meals or in lick blocks as supplements to straw the UMS gave the highest straw in take and digestion and live weight gains of growing bulls concurring the finding that the UMS system may be the best way of molasses and urea feeding to ruminants fed straws.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.9
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• pp.1248-1254
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• 2012

The effect of silage additives on ensiling characteristics and nutritive value of Napier grass (Pennisetum purpureum) silages was studied. Napier grass silages were made with no additive, fermented juice of epiphytic lactic acid bacteria (FJLB), molasses or cassava meal. The ensiling characteristics were determined by ensiling Napier grass silages in airtight plastic pouches for 2, 4, 7, 14, 21 and 45 d. The effect of Napier grass silages treated with these additives on voluntary feed intake, digestibility, rumen fermentation and microbial rumen fermentation was determined in 4 fistulated cows using $4{\times}4$ Latin square design. The pH value of the treated silages rapidly decreased, and reached to the lowest value within 7 d of the start of fermentation, as compared to the control. Lactic acid content of silages treated with FJLB was stable at 14 d of fermentation and constant until 45 d of ensiling. At 45 d of ensiling, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of silage treated with cassava meal were significantly lower (p<0.05) than the others. In the feeding trial, the intake of silage increased (p<0.05) in the cow fed with the treated silage. Among the treatments, dry matter intake was the lowest in the silage treated with cassava meal. The organic matter, crude protein and NDF digestibility of the silage treated with molasses was higher than the silage without additive and the silage treated with FJLB. The rumen parameters: ruminal pH, ammonia-nitrogen ($NH_3$-N), volatile fatty acid (VFA), blood urea nitrogen (BUN) and bacterial populations were not significantly different among the treatments. In conclusion, these studies confirmed that the applying of molasses improved fermentative quality, feed intake and digestibility of Napier grass.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.715-722
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• 1999

On a urea-molasses-straw (3:15:82, UMS) based diet, effect of graded levels of mustard oil cake (MOC) supplementation on the performances of native bulls has been studied. Four cannulated adult Bos indicus bulls of $415({\pm}44.6)kg$ live weight and 80 months old, were given daily either of 0, 200, 400 or 800 g of MOC in four periods in a $4{\times}4$ latin square design. Besides, each animal also received 200 g of each of molasses and wheat bran and a mineral mixture. For unit (1 g) increase in MOC intake, total DM intake increased by 0.8 g/d ($r^2=0.88$) but no change in the straw DM intake. With the increasing levels of MOC, crude protein (CP) digestibility increased exponentially with an asymptotic value of 72%. However, MOC level had no effect on the digestibilities of DM, OM and ADF. Similarly, rumen degradability of rice straw was also not affected by the level of dietary MOC, and mean straw DM degradabilities were 15, 21. 28, 37, 47 and 51% at 8, 16, 24, 48, 72 and 96 hours of incubation respectively. Microbial N yield per kg digestible organic matter apparently fermented in the rumen were 7.46, 8.77, 6.88 and 5.96 g respectively for 0, 200, 400 or 800 g of dietary MOC. For each gram increase in dietary MOC, N intake and N balance increased by 0.054 g/d ($r^2=0.998$) and $0.59mg\;N/kg\;W^{0.75}/d$ ($r^2=0.99$) respectively. Nitrogen balance was estimated to be attained at the N intakes of $246mg\;N/kg\;W^{0.75}/d$. Thus, on a UMS-based diet supplementation of MOC up to 800 g (10% of total intake) of the dietary intake had little or no effect on intake, digestibility, rumen parameters, and microbial N yield but slightly increased the N balance. However marginal response to MOC supplementation is probably due to the high degradability of MOC protein in the rumen. Thus, any substantial positive response of MOC supplementation on a UMS-based diet can probably be achieved by reducing its protein degradability in the rumen.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.1007-1014
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• 2019

Objective: This study was conducted to evaluate the fermentation characteristics under low mesophilic temperature of spent instant coffee ground (SICG) and to estimate the effect of fermented SICG (FSICG) as alternative feed ingredient on milk productivity of dairy cows. Methods: In the fermentation trial, fermentation of SICG was performed to investigate changes in characteristics using the microbial mixture (Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis = 1:1:1) for 21 days at $20^{\circ}C$ under anaerobic conditions. Molasses was added at 5% of dry mass. In the animal trial, eighteen Holstein Friesian cows were used to evaluate the nutritive value of the FSICG which was fermented for 14 days under the same condition as the fermentation trial. Results: In the fermentation trial, the dry matter (DM) and organic matter content linearly decreased with fermentation time (p<0.001 and p = 0.008, respectively). The acid detergent insoluble nitrogen content linearly decreased with fermentation time (p = 0.037). The microorganism counts linearly increased for Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis across fermentation time (p<0.001). In the animal trial, the DM intake of the control and FSICG treatment were not significantly different, as were milk yield, 4% fat corrected milk, fat-protein corrected milk, and feed to milk conversion content. Fat, protein, lactose, non-fat solids, milk urea nitrogen, and somatic cell counts were also not significantly different in milk composition between treatments. Conclusion: FSICG should be considered a sufficient substitute for cottonseed as a feed component, and 5% DM of a dietary FSICG level was appropriate for dairy cow diets.

• Animal Bioscience
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• v.34 no.3_spc
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• pp.371-384
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• 2021

Objective: Wheat bran (WB) was co-fermented with Aspergillus oryzae and phytase (Phy) to determine whether co-fermentation improve WB phosphorus and fiber utilization in Isa-brown layers. Methods: A total of 112 Isa brown layer were randomly divided into 7 treatments with 8 replicates per a treatment and 2 hens per a replicate. The treatments included basal diet (control), basal diet supplemented with 250 unit/kg Phy (control+Phy), diet with 10% WB (10% WB), diet with 5% WB and 250 unit/kg Phy (5% WB+Phy) diet with 10% WB and 250 unit/kg Phy (10% WB+Phy), diet with 5% fermented WB supplemented with molasses and phy (PCFWH) and 125 unit/kg Phy (5% PCFWH), and diet with 10% PCFWH (10% PCFWH). The intestinal microbial population, intestinal morphology, serum antioxidant enzyme activities, and excreta phosphorus content were assessed. Results: In PCFWH, spore counts, protease activity, xylanase activity, and ferulic acid were 8.50 log/g dry matter (DM), 190 unit/g DM, 120 unit/g DM, and 127 ㎍/g, respectively. Xylobiose and xylotriose were released in PCFWH, while they were not detectable in WB. Antioxidant capacity was also enhanced in PCFWH compared to WB. The 10% WB+Phy and 10% PCFWH groups produced higher egg mass, but hens fed 5% WB+Phy had the lowest amount of feed intake. Eggs from 10% PCFWH had better eggshell weight, eggshell strength, and eggshell thickness. Birds fed with 10% PCFWH also had higher serum superoxide dismutase and catalase activities. Compare to control, 10% PCFWH significantly reduced excreta phosphorus content. Conclusion: Diet inclusion of 10% PCFWH improved egg quality, antioxidant status, and excreta phosphorus content of laying hens.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.3
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• pp.326-332
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• 2001

On a urea-molasses-straw (3:15:82; UMS) based diet effect of graded levels of cottonseed cake (CSC) supplementation on the performance of native (Bos indicus) bulls has been studied for 167 days. Eighteen growing bulls of $129{\pm}13.4kg$ weight and about 14 months old were randomly allocated to three dietary treatments designed in a completely randomized design, having six animals in each treatment. Three dietary treatments were 0, 0.5 and 1.0 kg CSC per head/d. In addition, each animal also received ad lib. UMS, 4 kg Napier (Pennisetum purpureum) grass, 500 g of each of rice and wheat bran and 60 g mineral mix daily. For unit increase in CSC, total DM intake was increased by $1g/kg\;W^{0.75}/d$ but the straw DM intake decreased by $0.54g/kg\;W^{0.75}/d$. Whole gut digestibility of DM and OM was not effected but N and ADF digestibility increased with incremental increase in dietary CSC. For unit (1kg) increase in dietary CSC intake N and ADF digestibility increased by 10 (${\pm}1.155$) and 3 (${\pm}1.732$) unit respectively. Microbial N yield for the 0, 0.5 and 1.0 kg CSC were 5.63, 5.28 and 5.16 g/kg OM apparently fermented in the rumen respectively. For each gram increase in CSC, N intake and N balance increased by 0.626 (${\pm}0.015$) and 0.625 (${\pm}0.0814$) mg/kg $W^{0.75}/d$. High apparent N balance was contrasted with low live weight gain, e.g., for 1 kg increase in CSC supplementation, live weight gain increased by only 0.077 (${\pm}0.00288$) kg/d ($r^{2}=0.99$; p<0.01). The conversion efficiency was 12.98 kg CSC per kg of live weight gain. It was concluded that unless the protein is being protected from the rumen degradation, addition of CSC to UMS diet would have little nutritional or economic advantages.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.201-208
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• 2014

This study was conducted to evaluate the ensiling characteristics and the in situ degradability of a by-product feed (BF)-based silage. Before ensilation, the BF-based mixture was composed of 50% spent mushroom substrate, 21% recycled poultry bedding, 15% ryegrass straw, 10.8% rice bran, 2% molasses, 0.6% bentonite, and 0.6% microbial inoculant on a wet basis and ensiled for up to 4 weeks. The BF-based silage contained on average 39.3% moisture, 13.4% crude protein (CP), and 52.2% neutral detergent fiber (NDF), 49% total digestible nutrient, and 37.8% physically effective $NDF_{1.18}$ on a dry matter (DM) basis. Ensiling the BF-based silage for up to 4 weeks affected (p<0.01) the chemical composition to a small extent, increased (p<0.05) the lactic acid and $NH_3$-N content, and decreased (p<0.05) both the total bacterial and lactic acid bacterial counts from $10^9$ to $10^8$ cfu/g when compared to that before ensiling. These parameters indicated that the silage was fermented and stored well during the 4-week ensiling period. Compared with rice or ryegrass straws, the BF-based silage had a higher (p<0.05) water-soluble and filterable fraction, a lower insoluble degradable DM and CP fraction (p<0.05), a lower digestible NDF (p<0.05) fraction, a higher (p<0.05) DM and CP disappearance and degradability rate, and a lower (p<0.05) NDF disappearance and degradability rate. These results indicated that cheap, good-quality BF-based roughage could be produced by ensiling SMS, RPB, rice bran, and a minimal amount of straw.