• 제목/요약/키워드: Microbial survival

검색결과 118건 처리시간 0.025초

대기입자상물질의미생물메타게놈: 분석방법, 특성및영향인자 (Microbial Metagenome of Airborne Particulate Matter: Methodology, Characteristics, and Influencing Parameters)

  • 강수경;조경숙
    • 한국미생물·생명공학회지
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    • 제50권2호
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    • pp.165-192
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    • 2022
  • 본 논문에서는 실외 대기 환경의 바이오에어로졸 혹은 입자상물질의 미생물 메타게놈 특성과 이에 영향을 미치는 기후 및 환경 인자의 영향을 고찰하였다. 시료 채취 지역 및 환경 조건 특성별 대기 중 세균과 곰팡이 농도를 요약 하고, 에어로졸과 PM 시료의 세균과 곰팡이의 메타게놈 특성을 조사하기 위한 비배양법 기반 분석방법과 메타게놈 특성을 정리하였다. 또한, 세균과 곰팡이의 메타게놈 특성과 다양성 및 특성에 미치는 기상 인자와 환경 인자의 영향을 고찰하였다. 대기 중 미생물의 생존, 생장과 분산은 지역 기상 조건 및 대기 오염 물질에 의해 크게 영향을 받았다. 일반적으로 기온이 상승함에 따라 AM 농도는 증가하지만, 여름에는 고온과 강한 자외선의 영향으로 AM 농도가 감소하였다. 습도와 미생물 농도는 양의 상관성을 보이나, 습도가 너무 높으면 AM의 분산이 지연되었다. 이러한 종합적인 고찰 결과는 대기권에서 미생물의 역할과 기능을 이해하고, 이들 미생물에 의해 야기되는 환경 및 공중보건 문제를 해결하기 위한 전략 수립 및 저감 기술 개발에 활용될 수 있다.

활성탄에 침착시킨 $TiO_2$와 ZnO가 자외선에 의하여 활성화되었을때 Escherichia coli의 살균효과에 미치는 영향에 관한 연구 (The effects of UV excited $TiO_{2}$ and ZnO coating on activated carbon for Escherichia coli)

  • 최명신;정문호;김영규
    • 환경위생공학
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    • 제10권3호
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    • pp.105-114
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    • 1995
  • There has been increasing awareness on the importance of not only removal of organic materials but also sterilization of microbial cell in the drinking water purification research, so there has been many researches on that area. This study has been designed to analyze the effects of $TiO_{2}$ and ZnO coated on activated carbon on Escherichia coli. In this study, the sterilization power was analyzed by (1) variation of $TiO_{2}$ and ZnO concentration coated on activated carbon (2) variation of UV intensity. In addition, the kinetics between exposure time and sterilization velocity was viewed by the method of Chick. The results are as follows. 1. Survival ratio of E. coli decreased as time goes on in application of $TiO_{2}$, ZnO and $TiO_{2}{\cdot}ZnO$. In $TiO_{2}$ and ZnO, the effect increased upto certain concentration, but decreased there-after. In $TiO_{2}{\cdot}ZnO$, the effect of sterilization was in similar way among 3 combinations. 2. Survival ratio of E. coli decreased proportionately to an increase of light intensity in ZnO and $TiO_{2}{\cdit}ZnO$. In $TiO_{2}$, the survival ratio differed over extent of irradiation but the difference over the light intensity was not significant. 3. When Chick's law of sterilization was applied, m values of three concentrations of $TiO_{2}$ were 1.57,0.98, 1.96 respectively. M values of three concentration of ZnO were 1.10, 1.18,0. 11 respectively and those of three combination of $TiO_{2}{\cdot}ZnO$ were 1.17, 1.24, 1.74 respectively.

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무복토 직파에 의한 석탄 폐광지의 생태적 복원 기술 개발 (Development of the Ecological Restoration Technique using Direct Seeding without Soil Molding in Abandoned Coal Mine Areas)

  • 정용호;임주훈;이임균;김혜수
    • 한국환경복원기술학회지
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    • 제12권6호
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    • pp.76-85
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    • 2009
  • This study was carried to select the proper vegetation base materials which improve soil quality in abandoned coal mine areas. Also, we aimed at the feasibility of the direct seeding method without soil molding for the ecological restoration in those areas. We set total eight plots within the study site established on an abandoned coal mine area near Taebaek city, Gangwon province in April 2006. The plots were classified as two groups(straw mats mulching and no mulching), and the four treatments (C; control, MI; microbial innoculation, WC; wood chip, OF; organic fertilizer) were applied in each two groups for the soil conditioning. The survival of Pinus densiflora was highest among other species(Betula platyphylla var. japonica, Amorpha fruticosa and Arundinella hirta). For the non straw mat, the survival rate of Pinus densiflora seedlings was highest in the WC treatment($1,756trees/m^2$). For the straw mat, survival rate of Pinus densiflora seedlings was also highest in the WC treatment ($1,622trees/m^2$). In addition, for the non straw mat, the height growth of Pinus densiflora seedling was highest in the OF treatment($12.4{\pm}3.9cm$). For the straw mat, the height growth of Pinus densiflora seedling was also highest in the OF treatment($18.7{\pm}5.3cm$). In general, organic fertilizer treatment with the straw mat was most effective for seedling growth. Also, we suggested that the direct seeding method without soil molding could be sufficiently possible for revegetating abandoned coal mine, Korea.

Trypanosome Glycosylphosphatidylinositol Biosynthesis

  • Hong, Yeon-Chul;Kinoshita, Taroh
    • Parasites, Hosts and Diseases
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    • 제47권3호
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    • pp.197-204
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    • 2009
  • Trypanosoma brucei, a protozoan parasite, causes sleeping sickness in humans and Nagana disease in domestic animals in central Africa. The trypanosome surface is extensively covered by glycosylphosphatidylinositol (GPI)-anchored proteins known as variant surface glycoproteins and procyclins. GPI anchoring is suggested to be important for trypanosome survival and establishment of infection. Trypanosomes are not only pathogenically important, but also constitute a useful model for elucidating the GPI biosynthesis pathway. This review focuses on the trypanosome GPI biosynthesis pathway. Studies on GPI that will be described indicate the potential for the design of drugs that specifically inhibit trypanosome GPI biosynthesis.

Phototrophic Bacteria as Fish Feed Supplement

  • Banerjee, S.;Azad, S.A.;Vikineswary, S.;Selvaraj, O.S.;Mukherjee, T.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권7호
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    • pp.991-994
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    • 2000
  • Single cell of an indigenous phototrophic bacterium, Rhodovulum sulfidophilum, was incorporated in commercial fish feed for Oreochromis niloticus. The bacterial cell was analyzed for nutritional value and tested for toxicity and acceptability as an aquaculture feed supplement. The results showed higher survival rate and significantly higher growth rate (p<0.001) in O. niloticus fed with the bacteria incorporated fish feed. It is suggested that R sulfidophilum can be utilized as an aquaculture feed supplement.

Estimating the Viability of Bifidobacterium longum in Ca-Alginate Beads Against Simulated Gastroenteric Juices

  • Lee, Ki-Yong;Kim, Ji-Youn;Lee, Yoon-Jong;Choi, Eon-Ho;Shin, Dong-Hoon;Heo, Tae-Ryeon
    • Journal of Microbiology and Biotechnology
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    • 제11권1호
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    • pp.97-105
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    • 2001
  • The viability of Bifidobacterium longum KCTC 3128, entrapped in calcium alginate beds in simulated gastroenteric juices (gastric and bile salt solution), was tested to evaluate the influences of several parameters (gel concentration, bead size, and initial cell number). The death rate of B. longum in beads after being sequentially exposed to simulated gastric juices and bile salt solution decreased propertionally with increasing both the alginate gel concentration and bead size. The number of initial cell loading in beads affected the numbers of survivors after being exposed to these solutions, while the death rate of the viable cells were not affected. From the results obtained, the influence of entrapment parameters on the survival of bifidobacteria was quantitatively and systematically evaluated by using a mathematical method.

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Conserved Virulence Factors of Pseudomonas aeruginosa are Required for Killing Bacillus subtilis

  • Park Shin-Young;Heo Yun-Jeong;Choi Young-Seok;Deziel Eric;Cho You-Hee
    • Journal of Microbiology
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    • 제43권5호
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    • pp.443-450
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    • 2005
  • The multi-host pathogen, Pseudomonas aeruginosa, possesses an extraordinary versatility which makes it capable of surviving the adverse conditions provided by environmental, host, and, presumably, competing microbial factors in its natural habitats. Here, we investigated the P. aeruginosa-Bacillus subtilis interaction in laboratory conditions and found that some P. aeruginosa strains can outcompete B. subtilis in mixed planktonic cultures. This is accompanied by the loss of B. subtilis viability. The bactericidal activity of P. aeruginosa is measured on B. subtilis plate cultures. The bactericidal activity is attenuated in pqsA, mvfR, lasR, pilB, gacA, dsbA, rpoS, and phnAB mutants. These results suggest that P. aeruginosa utilizes a subset of conserved virulence pathways in order to survive the conditions provided by its bacterial neighbors.

An Advanced Understanding of Uterine Microbial Ecology Associated with Metritis in Dairy Cows

  • Jeon, Soo Jin;Galvao, Klibs N.
    • Genomics & Informatics
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    • 제16권4호
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    • pp.21.1-21.7
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    • 2018
  • Metritis, the inflammation of the uterus caused by polymicrobial infections, is a prevalent and costly disease to the dairy industry as it decreases milk yield, survival, and the welfare of dairy cows. Although affected cows are treated with broad-spectrum antibiotics such as ceftiofur, endometrial and ovarian function are not fully recovered, which results in subfertility and infertility. According to culture-dependent studies, uterine pathogens include Escherichia coli, Trueperella pyogenes, Fusobacterium necrophorum, and Prevotella melaninogenica. Recent studies using high-throughput sequencing observed very low relative abundance of Escherichia coli, Trueperella pyogenes, and Prevotella melaninogenica in cows with metritis. Herein, we propose that metritis is associated with a dysbiosis of the uterine microbiota, which is characterized by high abundance of Bacteroides, Porphyromonas, and Fusobacterium.

전라북도 지역 농업용수의 미생물학적 특성 및 온도와 수질에 따른 농업용수의 병원성대장균 O157:H7 밀도 변화 (Microbiological Quality of Agricultural Water in Jeollabuk-do and the Population Changes of Pathogenic Escherichia Coli O157:H7 in Agricultural Water Depending on Temperature and Water Quality)

  • 황인준;함현희;박대수;채효빈;김세리;김황용;김현주;김원일
    • 한국환경농학회지
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    • 제38권4호
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    • pp.254-261
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    • 2019
  • 농업용수는 채소류의 식중독세균 오염의 주요 경로 중 하나임에도 불구하고 우리나라에서는 농업용수의 미생물학적 안전성 대한 기초 자료가 부족한 실정이다. 본 연구에서는 전라북도에서 2018년 4월, 7월, 10월에 31지점에서 수집한 지표수 시료와 2018년 4월 7월에 20지점에서 수집한 지하수 시료의 위생지표세균 밀도를 조사하였다. 지표수에서는 평균적으로 대장균군이 2.7±0.55 log CFU/100 mL, 분원성대장균군 1.9±0.71 log CFU/100 mL, 대장균 1.4±0.58 log CFU/100 mL로 나타났고, 7월에 가장 높은 밀도를 보였다. 지하수의 경우 평균적으로 대장균군이 1.9±0.58 log CFU/100 mL, 분원성대장균군 1.4±0.37 log CFU/100 mL, 대장균 1.0±0.33 log CFU/100 mL로 나타났고 조사시기 간의 유의한 차이가 나타나지 않았다. 총질소량(T-N), 질산성질소(NO3-N) 등 유기물 함량이 높은 용수에서 E. coli O157:H7의 생존이 연장되는 것으로 나타났다. 물에서의 E. coli O157:H7 감소율은 25℃>35℃>5℃>15℃ 순으로 높게 나타났다. 이러한 결과는 전북 지역 농업용수의 미생물학적 오염도와 수질과 온도가 E. coli O157:H7의 생존에 미치는 영향을 보여준다. 이러한 결과는 농업용수의 미생물학적 오염도를 예측하고 미생물 제어 기술 개발의 기초자료로서 활용될 수 있다.

해양 준설토를 이용한 인공염습지 현장시험구 조성 후 초기 환경변화 (Initial Change of Environmental factors at Artificial Tidal Flat Constructed Using Ocean Dredged Sediment)

  • 박소영;이인철;이병호;이자연;이용민;성기준
    • 한국해양환경ㆍ에너지학회지
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    • 제11권2호
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    • pp.63-69
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    • 2008
  • 해양 준설토를 재활용하여 인공염습지를 조성하기 위한 기초연구로서 준설토 배합비가 상이한 pilot-scale의 인공염습지 현장시험구를 조성하고 대표적인 염생식물인 갈대를 이식한 후 초기의 환경변화를 조사하였다. 이식된 갈대의 정착률은 준설토의 배합비가 높을수록 낮아졌으나 새로 자라난 갈대의 지상부 길이와 직경은 준설비 배합비별로 큰 차이가 나타나지는 않았다. 준설토 100%로 조성된 시험구내 토양의 강열감량과 COD 농도는 유기물의 유출입이 비교적 자유로운 열린계(open system)로 인해 증감을 반복하는 양상을 보이긴 하였으나 초기값에 비해 각각 9%와 30%씩 감소하는 경향을 보여주었다. 시험구 조성 이후 시간이 흐름에 따라 준설토 50%와 100%의 시험구에서는 종속영양세균 개체수가 증가하는 것으로 나타났다. 이식한 갈대의 정착, 100% 준설토 시험구내의 감소된 COD 함량 및 종속미생물의 증가 등 본 연구의 결과로 염생식물의 식재를 통한 인공염습지의 조성 및 생물학적 분해를 이용하는 해양 준설토의 친환경적인 처리 가능성을 확인할 수 있었다. 하지만 준설토를 이용한 인공염습지 조성가능성에 대한 정확한 평가를 위해서는 보다 다양한 항목에 대한 지속적인 모니터링이 필요한 것으로 판단된다.

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