• Title/Summary/Keyword: Microbial decomposition

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Quality Stability of High Pressure Boiled Extract of Ogol Chicken during Storage Periods (오골계 증탕액의 저장 및 관능 특성)

  • 채현석;안종남;유영모;박범영;조수현;김진형;이종문;최양일
    • Korean Journal of Poultry Science
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    • v.29 no.4
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    • pp.279-286
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    • 2002
  • This study was conducted to examine quality stability during storage periods, herb high pressure boiled extract(HPBE)(T$_1$), Korean Ogol chicken HPBE(T$_2$), cross-bred Ogol chicken HPBE(T$_3$), cross-bred Ogol chicken meat hydrolyzed with flavourzyme(T$_4$) were pouch packaged and stored at 37$\^{C}$. After each period, TBARS, VBN, pH, total microbial counts and sensory properties were determined and the results were as follows. There was no noticeable difference in TBARS value until 42 days at the ambient environment among the treatments, but T$_4$ showed a significantly higher TBARS value at 56 days. There was a tendency for a higher protein decomposition as storage time increased, and in particular at 56 days, T$_1$ group showed a significantly higher values than other groups. Given to the sensory properties in which overall sensory preference decreased after 42 day, it was considered that the maximum storage time for the extract was less than 42 days at 37$\^{C}$.

Effects of Functional Packaging Films on the Longevity of Cut Lily Oriental Siberia Exported (기능성 포장필름이 수출 절화 백합의 수명에 미치는 영향)

  • Ko, Euisuk;Song, Kihyeon;Cho, Suhyun;Jeon, Kyubae;Kim, Chanwoo;Kim, Jaineung
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.21 no.2
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    • pp.55-60
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    • 2015
  • Packaging of export cut lilies reduces physical damage during distribution like dropping, shock, vibration, compression and serves to protect from necrosis, microbial contamination and decomposition. Study on which packaging materials must be selected is necessary because it serves to direct effect maintaining quality and the degree of freshness. newspaper, perforated OPP film, Oriented Nylon film (ON), punched OPP film, OPP films are used in this study. Plant senescence of lilies was happened rapidly during storage at $25^{\circ}C$ rather than at $5^{\circ}C$. Also, water absorption, fresh weight at during storage $25^{\circ}C$ rather than at $5^{\circ}C$ In addition, fresh weight of lilies storage at $25^{\circ}C$ appears higher than at $5^{\circ}C$ all of the packaging materials except OPP because water absorption of lily storage at $5^{\circ}C$ appears higher than at $25^{\circ}C$, thereby suppressing the fresh weight reducing Also, effect on biological activity of lilies from shape of packaging material appears higher during storage at $5^{\circ}C$ than at $25^{\circ}C$. Compared with each packaging materials, newspaper packaging is the smallest effect on changes in biological activity of cut lilies during storage. Thus, the results demonstrate that flowering date are affected mainly by the storage temperature rather than packaging materials.

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Biotransformation of natural polyacetylene in red ginseng by Chaetomium globosum

  • Wang, Bang-Yan;Yang, Xue-Qiong;Hu, Ming;Shi, Li-Jiao;Yin, Hai-Yue;Wu, Ya-Mei;Yang, Ya-Bin;Zhou, Hao;Ding, Zhong-Tao
    • Journal of Ginseng Research
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    • v.44 no.6
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    • pp.770-774
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    • 2020
  • Background: Fermentation has been shown to improve the biological properties of plants and herbs. Specifically, fermentation causes decomposition and/or biotransformation of active metabolites into high-value products. Polyacetylenes are a class of polyketides with a pleiotropic profile of bioactivity. Methods: Column chromatography was used to isolate compounds, and extensive NMR experiments were used to determine their structures. The transformation of polyacetylene in red ginseng (RG) and the production of cazaldehyde B induced by the extract of RG were identified by TLC and HPLC analyses. Results: A new metabolite was isolated from RG fermented by Chaetomium globosum, and this new metabolite can be obtained by the biotransformation of polyacetylene in RG. Panaxytriol was found to exhibit the highest antifungal activity against C. globosum compared with other major ingredients in RG. The fungus C. globosum cultured in RG extract can metabolize panaxytriol to Metabolite A to survive, with no antifungal activity against itself. Metabolites A and B showed obvious inhibition against NO production, with ratios of 42.75 ± 1.60 and 63.95 ± 1.45% at 50 µM, respectively. A higher inhibitory rate on NO production was observed for Metabolite B than for a positive drug. Conclusion: Metabolite A is a rare example of natural polyacetylene biotransformation by microbial fermentation. This biotransformation only occurred in fermented RG. The extract of RG also stimulated the production of a new natural product, cazaldehyde B, from C. globosum. The lactone in Metabolite A can decrease the cytotoxicity, which was deemed to be the intrinsic activity of polyacetylene in ginseng.

Studies on Degradation of Butachlor and Nitrofen in Different Soil Conditions (토양중(土壤中) Butachlor 와 Nitrofen의 분해(分解)에 관(關)한 연구(硏究))

  • Oh, Byung-Youl;Jeong, Young-Ho;Lee, Byung-Moo
    • Applied Biological Chemistry
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    • v.24 no.2
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    • pp.112-119
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    • 1981
  • The persistence of preemergence herbicides, butachlor [2-chloro-2,6-diethyl-N(butoxymethyl) acetanilide] and nitrofen(2,4-dichlorophenyl-4-nitrophenyl ether), at 10 ppm level in different soil conditions amended with organic matter and lime was studied under flooded and field moisture capacity. The microbial breakdown played a major role in the dissipation of the herbicides in soil. Nitrofen degradation in flooded soil was greatly accelerated, while it was slowed down in field moisture capacity as compared with butachlor. Increased amendment of rice straw to the soil shortened the half-life of butachlor under flooded condition, however it prolonged that of butachlor when the amendment was exceeded over 1% on dry weight basis. Liming the soil stimulated decomposition of the herbicides in the soil systems, which ap pears to be pH independent.

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Studies on the Microbial Decomposition of Cellulosic Materials - Part Ⅰ. Isolation of Cellulase-producing Microorganisms and Characterization of the Enzyme Activities - (섬유소분해(纖維素分解)의 미생물학적(微生物學的) 연구(硏究) - 제1보(第一報). 섬유소분해(纖維素分解) 미생물(微生物)의 분리(分離) 및 효소특성연구(醉素特性硏究) -)

  • Kim, Kyo-Chang;Kim, Chi-Kyung;Kim, Chang-Han
    • Applied Biological Chemistry
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    • v.24 no.2
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    • pp.85-93
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    • 1981
  • For the utilization of natural cellulosic materials by microorganisms, a potent cellulase-producing microorganism was isolated and identified as Trichoderma spp. Rice straw used as a substrate in this study was preliminarily treated with chemical solvents and/or additionally treated with acids and by heat, and then examined with the cellulase produced by the organism. Better results in sugar production by decomposing the straw cellulose were obtained, when the cellulase was produced by cultivating the organism in the selection medium, pH 5.0, for 5 days, and when the pretreated straw substrate was additionally treated with 0.1% $H_2SO_4$ sulfuric acid at $120^{\circ}C$ for 1 hour. The enzyme production was increased by about 20%, when 0.5% urea 0.5% phosphate, 0.1% meat extract, or 5% orange peel was added into the culture medium. For the practical purposes, the sugar production from the rice straw by the cellulase-producing microorganism can be improved by extending the reaction time of the enzyme up to 24 hr or longer.

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The Distribution of phosphorus in the Gomso Bay Tidal Flat (곰소만 조간대에서 인의 시공간적 분포)

  • 양재삼;김영태
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.7 no.3
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    • pp.171-180
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    • 2002
  • The temporal and spatial distributions of phosphorus have been investigated in the Gomso Bay, Korea. TP, PIP, TOP and DIP in sediment were found 548.8mg P kg$^{-1}$ , 426.1mg P kg$^{-1}$ , 122.6mg P kg$^{-1}$ , and 0.217mg P kg$^{-1}$ , respectively with a decreasing order of PIP>TOP>DIP. Any temporal or spatial trend has not been found on the distribution of TP in the sediment, except the high TP values near the mouth of Julpo-chun. We found seasonal patterns high TOP(28.90% of TP) and low TIP(71.10% of TP) in August, but low TOP(15.63% of TP) and high TIP(84.38% of TP) in November. There were three times higher DIP concentration in August than in November. Such case is probably not only due to the enhanced supply of DIP directly from the decomposition of organic matter from overlying water in summer, but also the released phosphate from the adsorbed particulate matter such as PIP under the low pH and Eh conditions at the subsurface layers of the sediment induced by the active microbial respiration of increased organic materials in summer. Primarily, the source of phosphorous from municipal sewage strongly influenced the early stage of the distribution of all the phosphorous in the Gomso tidal flat. Notwithstanding, through the processes of diagenesis in sediment, water temperature and organic contents probably functioned as the key parameters to control the temporal distributions of TOP, TIP and DIP in the Gomso tidal flat.

The Study of TCE Dechlorination using Geobacter lovleyi with Slow Release Substrate Applied (Slow Release Substrate를 이용한 Geobacter lovleyi의 TCE 탈염소화 연구)

  • Cha, Jae Hun;An, Sang Woo;Park, Jae Woo;Chang, Soon Woong
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.9
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    • pp.53-59
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    • 2012
  • This study investigated characteristics of decomposition of tetrabutoxysilane (TBOS) as a slow release substrate (SRS) and on effect of TBOS decompostion compounds (acetate and butylate) for anaerobic dechlorination of trichloroethylene (TCE). In the batch experiment, TCE, cis-dichloroethene (cis-DCE), 1-butanol and TBOS were analysed by GC/FID and acetate and butylate were measured by HPLC. 1M of TBOS transferred and accumulated 4M of 1-butanol by abiotically hydrolysis reaction. The hydrolysis rate was in a range of 0.186 ${\mu}M/day$. On other hand, 1-butanol fermented to butyrate and acetate with indigenous culture from natural sediments. This results showed that TBOS could be used a slow release substrate in the natural sites. The dechlorinated potential of TCE with acetate and butyrate was increased with a decreasing initial TCE concentrations. In addition, first order coefficients of dechlorination with acetate as electron donor was higher then that with butyrate. It is because that dechlorination of Geobacter lovleyi was affected by substrate affinity, biodegradability and microbial acclimation on various substrates. However, dechlorinated potential of Geobacter lovleyi was decreased with accumulation cis-DCE in the anaerobic decholoronation process. The overall results indicated that SRS with Geobacter lovleyi might be a promising material for enhancing dechlorination of TCE on natural site and cis-DCE should be treated by ZVI as reductive material or by coexisting other dechlorinated bacteria.

Hydrolytic and Metabolic Capacities of Thermophilic Geobacillus Isolated from Litter Deposit of a Lakeshore (수변 낙엽퇴적층에서 분리한 호열성 Geobacillus의 물질 분해 특성)

  • Baek, Hyun-Ju;Zo, Young-Gun;Ahn, Tae-Seok
    • Korean Journal of Microbiology
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    • v.45 no.1
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    • pp.32-40
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    • 2009
  • To understand contribution of thermophilic microorganisms in decomposition of litter deposits on shore of lakes, we surveyed a lakeshore litter deposit for bacteria growing at $60^{\circ}C$. Ten thermophilic isolates were selected for in-depth characterization, based on their high capacity to degrade high molecular weight organic compounds. Based on phylogenetic analysis on their 16S rRNA gene sequences, all isolates were identified as Geobacillus. The optimal growth temperature and pH of the strains ranged $55{\sim}60^{\circ}C$ and 6.0${\sim}$8.0, respectively. Salinity was inhibitory to the growth of the isolates, showing marked decrease of growth rates at 3% salinity. Based on activities of hydrolytic enzymes and profiles of carbohydrate utilization (determined by API 50 CHB kit), three G. stearothermophilus strains showed patterns clearly distinctive from other isolates. Two G. kaustophilus strains also demonstrated distinctiveness in their metabolic pattern and ecological parameters. However, ecological and metabolic profiles of the other five isolates were more variable and showed some degree of digression from their phylogenetic classification. Therefore, it could be concluded that endospore-forming thermophilic bacteria in lakeshore litter deposits contribute to degradation of organic materials with diverse ecological niches while having successions similar to microbial flora in compost. We propose that the thermophilic isolates and/or their thermo-tolerant enzymes can be applied to industrial processes as appropriate mixtures.

The Effect of Physico - Chemical Environmental Parameters on Early Composting of Paper - Mill Sludge Cakes (이화학적 환경변수가 제지슬러지케이크의 조기부숙에 미치는 영향)

  • 최홍림;김현태;하호성;정영윤;남상일;서형덕;정원준
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.34 no.3
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    • pp.75-84
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    • 1992
  • The paper manufactories in Sangpyeong Industrial Estates, Chinju, produce more than 80 tons of paper-mill sludge cakes every day, which costs about 840 million won for dump per year. Since the paper-mill sludge is biodegradable, the sludge can he utilized as an organic fertilizer if it is properly decomposed. This may lead to not only dramatic cut of the treatment cost but prevention from secondary environmental contamination due to dump. The primary objective of the study was to explore the quantitative range of major enviromental parameters influencing early composting of paper-mill cakes, naturally pretreated in warn and cold weather. The automatically-controlled reactor was designed, manufactured, and operated for nine days to decompose about 2201 of raw sludge cakes. Four tests(Test 1-Test 4) were implemented for the study of Phase I. Treatments of two levels of initial temperature (40˚C and 28˚C) and two levels of water content + C/N ratio (35% + 40 and 63% + 80) were made to test the significance of their parameters for decomposition of raw sludge cakes pretreated in warm weather. Another four tests (Test 5- Test 8) were implemented for the study of Phase II. Treatments of initial temperature and water content(W/C) + C/N ratio of raw sludge pretreated in cold weather were made to 16˚C and 13% +58 for Test 5, 6˚C and 53% +55 for Test 6, 7˚C and 36% +81 for Test 7, 31˚C and 30% +81 for Test 8. Natural weater condition(pretreatment condition) revealed the importance m composting of the paper-mill sludge cakes. Combination of water content adjustment to about 30% with C/N ratio amendment of about 20 and initial temperature of 30~40˚C was concluded to be the best for early composting of paper-mill sludge cakes with aeration rate and pH fixed. Temperature and C/N ratio were adapted as judging variables for composting degree. In addition, tests for microbial activity were performed to validate the experimental results. Since the temperature and C/N ratio did not coincide in some tests as judging variables for the maturity of the composting sludges, taking one of these parameters could mislead the concept of the maturity (composting conceptually new criterion to provide more reliable information for early composting of paper-mill sludge cakes.

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Designed of rPP/d2w®/ZnO Nanocomposite Flexible Film for Food Packaging and Characterization on Mechanical and Antimicrobial Properties (산화분해촉매를 함유한 rPP/ZnO 나노컴포지트 유연식품포장필름 제조 및 물성 특성 연구)

  • Lee, Jin-kyoung;Gil, Bo-min;Lee, Dong-jin;Lee, Ik-mo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.24 no.1
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    • pp.1-11
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    • 2018
  • In this study, pro-oxidant($d2w^{(R)}$) and rPP/ZnO nanocomposite flexible films for food packaging were prepared, and their mechanical and antimicrobial properties were investigated. As a result, the carbonyl index and hydroxyl index increased with exposured time to heat and UV rays. Surface analysis showed that the addition of zinc oxide improved the dispersibility and compatibility of the polymer, so that the surface of the composite film was smooth and the zinc oxide particles were smaller than the compared film. And it kept the physical properties by heat and UV ray blocking effect, and it worked to reduce decomposition. In the antimicrobial activity test, the microbial reduction rate was 3 logs or more at the use concentration of zinc oxide. The tensile strength was increased and the elongation was decreased. Oxidative degradability of multi-layered film in UV exposured for 72 hours, the molecular weight of the film decreased by 75.6%, 1,294 g/mol Mn and 5,920 g/mol Mw. In the safety analysis of food packaging materials, we obtained that are in standard of polypropylene, a food contact material of domestic law.