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The Study of TCE Dechlorination using Geobacter lovleyi with Slow Release Substrate Applied  

Cha, Jae Hun (경기대학교 환경에너지시스템공학과)
An, Sang Woo (한양대학교 건설환경공학과)
Park, Jae Woo (한양대학교 건설환경공학과)
Chang, Soon Woong (경기대학교 환경에너지시스템공학과)
Publication Information
Journal of the Korean GEO-environmental Society / v.13, no.9, 2012 , pp. 53-59 More about this Journal
Abstract
This study investigated characteristics of decomposition of tetrabutoxysilane (TBOS) as a slow release substrate (SRS) and on effect of TBOS decompostion compounds (acetate and butylate) for anaerobic dechlorination of trichloroethylene (TCE). In the batch experiment, TCE, cis-dichloroethene (cis-DCE), 1-butanol and TBOS were analysed by GC/FID and acetate and butylate were measured by HPLC. 1M of TBOS transferred and accumulated 4M of 1-butanol by abiotically hydrolysis reaction. The hydrolysis rate was in a range of 0.186 ${\mu}M/day$. On other hand, 1-butanol fermented to butyrate and acetate with indigenous culture from natural sediments. This results showed that TBOS could be used a slow release substrate in the natural sites. The dechlorinated potential of TCE with acetate and butyrate was increased with a decreasing initial TCE concentrations. In addition, first order coefficients of dechlorination with acetate as electron donor was higher then that with butyrate. It is because that dechlorination of Geobacter lovleyi was affected by substrate affinity, biodegradability and microbial acclimation on various substrates. However, dechlorinated potential of Geobacter lovleyi was decreased with accumulation cis-DCE in the anaerobic decholoronation process. The overall results indicated that SRS with Geobacter lovleyi might be a promising material for enhancing dechlorination of TCE on natural site and cis-DCE should be treated by ZVI as reductive material or by coexisting other dechlorinated bacteria.
Keywords
Slow Release Substrate; TBOS; TCE; Cis-DCE; Geobacter lovleyi;
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