• Title/Summary/Keyword: Microbial component

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Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17

  • Kim, Doc-Kyu;Chae Jong-Chan;Zylstra Gerben J.;Sohn Ho-Yong;Kwon, Gi-Seok;Kim, Eung-Bin
    • Journal of Microbiology
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    • v.43 no.1
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    • pp.49-53
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    • 2005
  • Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

Removal Efficiency of Heavy Metals in Acidic Mine Drainage from Microbial Mats (바이오매트 형성에 의한 산성광산배수 내 중금속 유출질량 제거효율)

  • Yu, Hun-Sun;Kwon, Byung-Hyuk;Kim, Park-Sa;So, Yoon-Hwan;Kang, Dong-Hwan
    • Journal of Environmental Science International
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    • v.21 no.6
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    • pp.667-676
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    • 2012
  • This research investigated to reduce mass of heavy metals in AMD(acid mine drainage) by microbial mats formed on the channel bed. As, Cd, Cu, Fe, Mn and Zn components were monitored in water and microbial mats, at three points (AMD1, AMD2 and AMD3), in a total of six times. Average daily discharge mass of heavy metals was highest in July, Fe component contained more than 76% of total discharge mass. Discharge mass of heavy metals of AMD and heavy metal contents in microbial mats decreased with downstream at channel. Heavy metal components that average daily discharge mass is over 0.5 kg were Fe, Cu and Zn, and they were highest in July. Average removal efficiency of heavy metals in AMD was highest about 21% in Fe, this microbial mats were due to form from precipitation of Fe component in AMD by aerobic iron bacteria. Relative content for As component in microbial mats than AMD was over 16 times, this As components were due to absorb at iron oxide and iron hydroxide on the surface of microbial mats.

Statistical Analysis of Sewage Plant Operation (하수처리장 운전조건의 통계분석)

  • 이찬형;문경숙
    • Journal of Environmental Science International
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    • v.11 no.1
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    • pp.63-68
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    • 2002
  • In this study, we examined statistical analysis between sewage plant operations parameters and effluent quality We got six components from principle component analysis of the operation parameters and secondary effluent quality. 91.8% of the total variance was explained by the six components. The components were identified in the following order : 1) organic matter removal by aeration basin microbe, 2) settleability on secondary clarifier load, 3) removal of nutrients, 4) microbial number increasement and species diversity, 5) microbial activity in aeration basin, 6) oxidation in aeration basin.

Studies on the constituents of the higher fungi of Korea (XI) An Antibiotic Component and a Sterol of Coriolus sanguineus Fr.

  • Chung, Kyong-Soo;Shim, Mi-Ja;Kim, Byong-Kak
    • Archives of Pharmacal Research
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    • v.1 no.1
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    • pp.33-40
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    • 1978
  • From the carpophores of Coriolus sanguineus Fr., a bright-red wood-rotting fungus, which were collected at Suwon, an antibiotic component was isolated as red crystal and identified as cinnabarin by UV and IR spectra. The antibiotic activities against five bacteria and three fungi were determined. It showed considerably high activity against Bacillus subtilis ATCC 6633 and Bacillus maceraus and also against all the three fungi tested. And from this fungus a sterol was isolated and identified as erogosterol by cochromatographing with four authentic sterols.

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Inhibition of Transglutaminase and Microbial Transglutaminase Activity by Garlic

  • Lee, Nam-Hyouck;Takeuchi, Atsuyoshi;Konno, Kunihiko
    • Food Science and Biotechnology
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    • v.16 no.2
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    • pp.223-227
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    • 2007
  • Ground garlic inhibited the cross-linking reaction of myosin and incorporation of monodansylcadaverine (MDC) in salted Alaska pollack surimi catalyzed by transglutaminase (TGase). The component responsible for the inhibition was a thermostable, low molecular weight compound. The component also inhibited microbial transglutaminase (MTGase). The inhibition by garlic was reversibly recovered upon addition of 2-mercaptoethanol. The inhibitory component was therefore hypothesized to contain sulfhydryl groups within its structure. Alliin itself did not inhibit the cross-linking reaction. However, the addition of alliin together with garlic increased the inhibition. This result suggested that compounds derived from alliin was responsible for the inhibition of TGase activity.

Analysis of the Seasonal Change in Attached Algae and Microbial Community Structure in Sediment Basin Trough of Water Treatment Plant By Using Quinone Profile and PCR-DGGE (Quinone profile과 PCR-DGGE를 이용한 정수장 침전지에서의 부착조류 및 미생물군집의 계절적 변화해석)

  • Yu, Hyun-Sun;Lim, Byung-Ran;Ahn, Kyu-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.3
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    • pp.461-467
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    • 2006
  • The seasonal change in attached algae and microbial community structure at sedimentation basin of water treatment plant was investigated by using quinone profiles and denaturing gel gradient electrophoresis (DGGE). The photosynthetic bacteria and algae contains PQ-9 and VK-1 as major quinone are major component of the total quinone fraction in attached algae and microorganisms on sedimentation basin trough. The microorganisms containing menaquinones appear to be sensitivity to the change in temperature than those containing ubiquinones. The plot of the mole fraction of dominant quinone species ($f_d$) to the DQ values showed higher sensitivity to the seasonal change in the microbial community structure. The results indicated that quinone and DGGE are useful tool for the evaluation of the changes in the microbial community structure.

A Study on Methods of Separating Soil Respiration by Source (발생원별 토양 호흡 측정 연구 방법에 대한 고찰)

  • Koo Jin-Woo;Son Yowhan;Kim Rea-Hyun;Kim Joon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.1
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    • pp.28-34
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    • 2005
  • We review three methods of separating soil respiration into root and soil microbial contribution: (1) component integration, (2) root exclusion, and (3) isotopic method. Among these methods, component integration and root exclusion are most commonly used. Root respiration contribution to soil respiration estimated by the root exclusion method is higher than those by other two methods. Trenching has little environmental disturbances in soil or on surface of site compared to other methods in root exclusion such as root removal and gap formation. Isotopic method has an advantage over other methods because of minimal soil and root disturbances, but this method is costly and requires techniques for the complex analysis. Trenching seems to be an appropriate in situ method for calculating component contributions to soil respiration with minimum disturbances in site. However, the method overestimates the contribution of microbial respiration because of root decay, and realistic results could be obtained by estimating root decay or avoiding large roots in trenched plots.

Effect of Gamma Irradiation on the Microbial Safety and Biological Activities of Tuna Cooking Juices (감마선 조사에 따른 참치 자숙액의 위생화 및 기능성 변화 연구)

  • Byun, Myung-Woo
    • KSBB Journal
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    • v.27 no.4
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    • pp.222-226
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    • 2012
  • In this study, the effect of gamma irradiation on the microbial contamination and biological activities of tuna cooking juices was investigated. Tuna cooking juice was by-produced during the canning processing, and had various functional components. But, it was shown that the tuna cooking juice was seriously contaminated. Gamma irradiation effectively reduced the microbial population in tuna cooking juice. Also, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, tyrosinse inhibitory activity, and ACE inhibitory activity of tuna cooking juices were all increased as a result of gamma irradiation. These results suggest that wasted tuna cooking juices can be used as a functional component in the food and cosmetic industries if the irradiation technology were applied.

Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE (Biolog Ecoplate와 DGGE 방법을 이용한 알칼리화 토양의 미생물군집 변화 평가)

  • Lee, Eun Young;Hong, Sun Hwa
    • KSBB Journal
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    • v.28 no.5
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    • pp.275-281
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    • 2013
  • Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.

Effects of simulated acid rain on microbial activities and litter decomposition

  • Lim, Sung-Min;Cha, Sang-Seob;Shim, Jae-Kuk
    • Journal of Ecology and Environment
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    • v.34 no.4
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    • pp.401-410
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    • 2011
  • We assayed the effects of simulated acid rain on the mass loss, $CO_2$ evolution, dehydrogenase activity, and microbial biomass-C of decomposing Sorbus alnifolia leaf litter at the microcosm. The dilute sulfuric acid solution composed the simulated acid rain, and the microcosm decomposition experiment was performed at 23$^{\circ}C$ and 40% humidity. During the early decomposition stage, decomposition rate of S. alnifolia leaf litter, and microbial biomass, $CO_2$ evolution and dehydrogenase activity were inhibited at a lower pH; however, during the late decomposition stage, these characteristics were not affected by pH level. The fungal component of the microbial community was conspicuous at lower pH levels and at the late decomposition stage. Conversely, the bacterial community was most evident during the initial decomposition phase and was especially dominant at higher pH levels. These changes in microbial community structure resulting from changes in microcosm acidity suggest that pH is an important aspect in the maintenance of the decomposition process. Litter decomposition exhibited a positive, linear relationship with both microbial respiration and microbial biomass. Fungal biomass exhibited a significant, positive relationship with $CO_2$ evolution from the decaying litter. Acid rain had a significant effect on microbial biomass and microbial community structure according to acid tolerance of each microbial species. Fungal biomass and decomposition activities were not only more important at a low pH than at a high pH but also fungal activity, such as $CO_2$ evolution, was closely related with litter decomposition rate.