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Analysis of the Seasonal Change in Attached Algae and Microbial Community Structure in Sediment Basin Trough of Water Treatment Plant By Using Quinone Profile and PCR-DGGE  

Yu, Hyun-Sun (한국과학기술연구원 수질환경 및 복원 연구센터)
Lim, Byung-Ran (명지대학교 환경생명공학부)
Ahn, Kyu-Hong (한국과학기술연구원 수질환경 및 복원 연구센터)
Publication Information
Journal of Korean Society of Water and Wastewater / v.20, no.3, 2006 , pp. 461-467 More about this Journal
Abstract
The seasonal change in attached algae and microbial community structure at sedimentation basin of water treatment plant was investigated by using quinone profiles and denaturing gel gradient electrophoresis (DGGE). The photosynthetic bacteria and algae contains PQ-9 and VK-1 as major quinone are major component of the total quinone fraction in attached algae and microorganisms on sedimentation basin trough. The microorganisms containing menaquinones appear to be sensitivity to the change in temperature than those containing ubiquinones. The plot of the mole fraction of dominant quinone species ($f_d$) to the DQ values showed higher sensitivity to the seasonal change in the microbial community structure. The results indicated that quinone and DGGE are useful tool for the evaluation of the changes in the microbial community structure.
Keywords
Attached algae; DGGE; microbial community structure; quinone profile; sedimentation basin;
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