Browse > Article

Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17  

Kim, Doc-Kyu (Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Microbial Resources Bank, Microbial Genomics and Applications Center Korea Research Institute of Bioscience and Biotechnology)
Chae Jong-Chan (Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University)
Zylstra Gerben J. (Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University)
Sohn Ho-Yong (Department of Food and Nutrition, Andong National University)
Kwon, Gi-Seok (The School of Bioresource Science, Andong National University)
Kim, Eung-Bin (Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University)
Publication Information
Journal of Microbiology / v.43, no.1, 2005 , pp. 49-53 More about this Journal
Abstract
Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
Keywords
Rhodococcus; two-component signal transduction proteins; alkylbenzenes;
Citations & Related Records

Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 8
연도 인용수 순위
1 Asturias, J.A. and K.N. Timmis. 1993. Three different 2,3-dihydroxy biphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6. J. Bacteriol. 175, 4631-4640   DOI   PUBMED
2 Haddad, S., D.M. Eby, and E.L. Neidle. 2001. Cloning and expression of the benzoate dioxygenase genes from Rhodococcus sp. strain 19070. Appl. Environ. Microbiol. 67, 2507-2514   DOI   ScienceOn
3 Kim, D., Y-S. Kim, S-K. Kim, S.W. Kim, G.J. Zylstra, Y.M. Kim, and E. Kim. 2002. Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17. Appl. Environ. Microbiol. 68, 3270-3278   DOI   ScienceOn
4 Sakai, M., K. Miyauchi, N. Kato, E. Masai, and M. Fukuda. 2003. 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 69, 427-433   DOI   ScienceOn
5 Warhurst, A.M. and C.A. Fewson. 1994. Biotransformations catalyzed by the genus Rhodococcus. Crit. Rev. Biotechnol. 14, 29-73   DOI   ScienceOn
6 Kim, D., Y-S. Kim, J.W. Jung, G.J. Zylstra, Y.M. Kim, S.-K. Kim, and E. Kim. 2003. Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17. FEMS Microbiol. 223, 211-214   DOI   ScienceOn
7 Choi, K.Y., D. Kim, S.-C. Koh, J.-S. So, J.-S. Kim, and E. Kim. 2004. Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene. J. Microbiol. 42, 160-162
8 Labbe, D., J. Garnon, and P.C. Lau. 1997. Characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/polychlorobiphenyl-degrading bacterium, Rhodococcus sp. strain M5. J. Bacteriol. 179, 2772-2776   DOI   PUBMED
9 Stock, A.M., V.L. Robinson, and P.N. Goudreau. 2000. Two-component signal transduction. Annu. Rev. Biochem. 69, 183-215   DOI   ScienceOn
10 Walker, J.E., M. Saraste, M.J. Runswick, and N.J. Gay. 1982. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 945-951   PUBMED
11 Stecker, C., A. Johann, C. Herzberg, B. Averhoff, and G. Gottschalk. 2003. Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2. J. Bacteriol. 185, 5269-5274   DOI   ScienceOn
12 Kim, D., J-C. Chae, J. Y. Jang, G. J. Zylstra, Y. M. Kim, B. S. Kang, and E. Kim. 2005. Functional characterization and molecular modeling of methylcatechol 2,3-dioxygenase from o-xylenedegrading Rhodococcus sp. strain DK17. Biochem. Biophys. Res. Commun. 326, 880-886   DOI   ScienceOn
13 Volz, K. 1993. Structural conservation in the CheY superfamily. Biochemistry 32, 11741-11753   DOI   PUBMED   ScienceOn
14 Finnerty, W.R. 1992. The biology and genetics of the genus Rhodococcus. Annu. Rev. Microbiol. 46, 193-218   DOI   PUBMED   ScienceOn
15 Parkinson, J.S. and E.C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26, 71-112   DOI   ScienceOn
16 Wang, Y., J. Garnon, D. Labbe, H. Bergeron, and P. C. Lau. 1995. Sequence and expression of the bpdC1C2BADE genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by Rhodococcus sp. M5. Gene 164, 117-122   DOI   PUBMED   ScienceOn
17 Stanier, R.Y., N.J. Palleroni, and M. Duodoroff. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43, 159-271   DOI   PUBMED   ScienceOn
18 Kim, D., J-C. Chae, G. J. Zylstra, Y-S. Kim, S-K. Kim, M. H. Nam, Y. M. Kim, and E. Kim. 2004. Identification of a novel dioxygenase involved in metabolism of o-xylene, toluene, and ethylbenzene by Rhodococcus sp. strain DK17. Appl. Environ. Microbiol. 70, 7086-7092   DOI   ScienceOn
19 Larkin, M.J., R. De Mot, L.A. Kulakov, and I. Nagy. 1998. Applied aspects of Rhodococcus genetics. Antonie Van Leeuwenhoek. 74, 133-153   DOI   ScienceOn
20 Takeda, H., A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda. 2004. Characterization of transcriptional regulatory genes for biphenyl degradation in Rhodococcus sp. strain RHA1. J. Bacteriol. 186, 2134-2146   DOI   ScienceOn
21 Kesseler, M., E.R. Dabbs, B. Averhoff, and G. Gottschalk. 1996. Studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2. Microbiology 142, 3241-3251   DOI   ScienceOn
22 Mahenthiralingam, E. 1998. Extraction of RNA from mycobacteria. Methods Mol. Biol. 101, 65-75   PUBMED