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http://dx.doi.org/10.7841/ksbbj.2013.28.5.275

Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE  

Lee, Eun Young (Department of Environmental and Energy Engineering, The University of Suwon)
Hong, Sun Hwa (Department of Environmental and Energy Engineering, The University of Suwon)
Publication Information
KSBB Journal / v.28, no.5, 2013 , pp. 275-281 More about this Journal
Abstract
Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.
Keywords
Ash; Alkaline soil; Microbial community; Ecoplate; DGGE;
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1 Muyzer, G, E. C. De-Waal, and A. G. Uitterlinden (1993) Profiling of Complex Microbial Populations by Denaturing Gradient Gel Electrophoresis Analysis of Polymerase Chain Reaction-Amplified Genes Coding for 16S rRNA. Appl. Environ. Microbiol. 59: 695-700.
2 Amann, R. I., W. Ludwig, and K. H. Schleifer (1995) Phylogenetic Identification and in Situ Detection of Individual microbial Cells Without Cultivation. Microbial. Rev. 59: 143-169.
3 Head, I. M., J. R. Saunders, and R. W. Pickup (1998) Microbial evolution diversity, and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. Microbial Ecol. 35: 1-21.   DOI   ScienceOn
4 Knight, B.P., S. P. McGrath, and A. M. Chaudri (1997) Biomass carbon measurements and substrate utilization patterns of microbial populations from soils amended with cadmium, copper or zinc. Appl. Environ. Microbiol. 63: 39-43.
5 Bruns, M. A., J. R. Stephen, G. A. Kowalchuk, J. I. Prosser, and E. A. Paul (1999) Comparative Diversity of Ammonia Oxidizer 16SrRNA Gene Sequences in Native, Tilled, and Successional Soils. Appl. Envrion. Microbiol. 65: 2994-3000.
6 Duineveld, B. M., A. S. Rosado, J. D. Elsas, and J. A. Veen (1998) Analysis of the Dynamics of Bacterial Communities in the Rhizosphere of the Chrysanthemum via Denaturing Gradient Gel Electrophoresis and Substrate Utilization Pattern. Appl. Envrion. Microbiol. 64: 4950-4957.
7 Heuer, H. M., K. P. Baker, K. Smalla, and E. M. H. Wellington (1997) Analysis of actinomycete communities by specific amplification of genes encoding 16S-rRNA and gel-electrophoretic separation in denaturing gradients. Appl. Envrion. Microbiol. 63: 3232-3241.
8 Garland, J. L. and A. L. Mills (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community level sole carbon source utilization. Appl. Envrion. Microbiol. 57: 2351-2359.
9 Ian F. S. and J. F. Peter (2003). A Tribute to Claude Shannon (1916-2001) and a Plea for More Rigorous Use of Species Richness, Species Diversity and the Shannon-Wiener Index. Global Ecol. Biogeog. 12: 177-179.   DOI   ScienceOn
10 An, Y. J., Y. H. Joo, I. Y. Hong, H. W. Ryu, and K. S. Cho (2004) Microbial characterization of toluene-degrading denitrifying consortia obtained from terrestrial and marine ecosystems. Appl. Envrion. Microbiol. 65: 611-619.
11 Preston-Mafham, J., L. Boddy, and P. F. Randerson (2002) Analysis of microbial community functional diversity using sole-carbonsource utilisation profiles - A critique. FEMS Microbiol. Ecol. 42: 1-14.
12 Betancur-Galvis, L. A., D. Alvarez-Bernal, A. C. Ramos-Valdivia, and L. Dendooven (2006) Bioremediation of polycyclic aromatic hydrocarbon-contaminated saline-alkaline soils of the former Lake Texcoco. Chemosphere 62: 1749-1760.   DOI   ScienceOn
13 Bao, T., L. Sun, T. Sun, P. Zhang, and Z. Niu (2008) Iron-deficiency induces cadmium uptake and accumulation in Solanum nigrum L. Bull. Environ. Contam. Toxicol. 82: 338-342.
14 National Center for Biotechnology Information. http://blast.ncbi.nlm.nih.gov/Blast.cgi
15 Fisher, R. F. and D. Binkley (2000) Ecology and management of forest soils. pp. 489. John Wiley & Sons, New York.
16 Shoda, M. (2000) Bacterial control of plant diseases. J. Biosci. Bioeng. 89: 515-521.   DOI   ScienceOn
17 Sholberg, P. L., A. Marchi, and J. Bechard (1995) Biocontrol of postharvest diseases of apple using Bacillus spp. isolated from stored apples. Can. J. Microbiol. 41: 247-252.   DOI   ScienceOn
18 Subhash, C. V., K. L. Jagdish, and K. T. Anil (2001) Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. J. Biotechnol. 91: 127-141.   DOI   ScienceOn
19 Choi, D. S., H. O. Jin, D. J. Chung, K. Sasa, and T. Koike (2008) Growth and physiological activity in Larix kaempferi seedlings inoculated with ectomycorrhizae as affected by soil acidification. Trees 22: 729-735.   DOI   ScienceOn
20 Izuta, T., T. Yamaoka, T. Nakaji, T. Yonekura, M. Yokoyama, H. Matsumur, S. Ishida, K. Yazaki, R. Funada, and T. Koike (2001) Growth, net photosynthetic rate, nutrientstatus and secondary xylem anatomicalcharacteristics of Fagus crenata seedlingsgrown in brown forest soil acidified withH2SO4 solution. Water Air Soil Pollut. 130: 1007-1012.   DOI   ScienceOn
21 Krause, G. H. M., U. Arndt, G. J. Brandt, J. Bucher, G. Kent, and E. Matzner (1986) Forest decline in Europe: Development and possible causes. Water Air Soil Pollut. 31: 647-668.   DOI   ScienceOn
22 Giovannoni, S. J., T. B. Brischgi, C. L. Moyer, and K. S. Field (1990) Genetic Diversity in Sargasso Sea Bacterioplankton. Nature (London) 345: 60-63.   DOI   ScienceOn
23 Ward, D. M., R. Weller, and M. M. Bateson (1990) 16S rRNA Sequences Reveal Numerous Uncultured Microorganisms in a Natural Community. Nature 345: 63-65.   DOI   ScienceOn