• 상하수도학회지
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• 제20권6호
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• pp.885-892
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• 2006

In order to investigate whether or not Total Coliforms (T.C.) and Fecal Coliforms (F.C.) are compatible as indicator microorganisms of waterbome enteric viruses, a total of 192 surface water samples from 24 locations in Korea were tested for T.C., F.C., and human enteric viruses from July 2003 to January 2006. Altogether, the number of T.C. in each samples was ranged from $0{\sim}5.3{\times}10^4$ colony forming unit(CFU)/100mL, and the number of F.C. ranged from $0{\sim}5.0{\times}10^3CFU/100mL$ per sample. Thirty-three percent of the samples tested positive for human enteric viruses after the total culturable virus assay. The results of the statistical analysis showed that T.C. and F.C. had a significant correlation with turbidity and temperature, but the waterbome enteric viruses did not. When compared to the number of T.C. or F.C. per sample, the concentration of waterbome enteric viruses was not found to be correlated. In conclusion, it is suggested that T.C. and F.C. may not be sufficient microbial indicators of waterbome enteric viruses in the samples analyzed in this study. However, further research is needed to find other microbial indicators of waterbome enteric viruses and to develop more advanced and sensitive methods to detect waterborne enteric viruses.

• 대한한방부인과학회지
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• 제19권4호
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• pp.17-32
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• 2006

Purpose : The aim of this study is to investigate the antibiotic effects of 14 herbs among blood-activating stasis-dispelling medicinals on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans, and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal normal flora. The blood-activating stasis-dispelling medicinals, Mucunae Caulis, Salviae Radix, Persicae Semen, Myrrha, Zedoariae Rhizoma, Achuranthis Radix, Leonuri Herba, Melandrii Herba, Gleditsiae Spina, Lycopi Herba, Scirpi Rhizoma, Caesalpiniae Lignum, Corydlais Tuber and Polygoni Cuspidati Radix were used in this study. In vitro antibiotic activities were observed by optical density and colony test. Results : The optical density and colony test showed that Gleditsiae Spina, Scirpi Rhizoma, Corydlais Tuber, Polygoni Cuspidati Radix and Melandrii Herba of herbs among blood-activating stasis-dispelling medicinals had antimicrobial effect. Gleditsiae Spina had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis and MRSA. Scirpi Rhizoma had antimicrobial susceptibility and selective toxicity in Staphylococcus aureus and MRSA. Corydlais Tuber had antimicrobial susceptibility and selective toxicity in MRSA. Polygoni Cuspidati Radix had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis, Staphylococcus aureus and MRSA. Melandrii Herba had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Gleditsiae Spina, Scirpi Rhizoma, Corydlais Tuber, Polygoni Cuspidati Radix and Melandrii Herba of herbs among blood-activating stasis-dispelling medicinals be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

• 대한한방부인과학회지
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• 제19권3호
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• pp.175-190
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• 2006

Purpose : This study was conducted to investigate the in vitro inhibitory effects of heat-clearing medicinal on common bacterias in gynecology. Methods : The heat-clearing medicinals ( Trichosanthis Radix, Sophorae Fructus, Phragmitis Rhizoma, Buddleiae Flos, Bambusae Folium, Anemarrhenae Rhizoma, Celosiae Semen, Gardeniae Fructus, Prunellae Spica, Sophorae Radix, Dictamni Radicis Cortex, Coptidis Rhizoma, Gentianae Scabrae Radix, Scutellariae Radix, Phellodendri Cortex) were used in this study. Staphylococcus aureus, MRSA, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Streptococcus spp., Escherichia coli HB101, Lactobacillus gasseri were used for normal vaginal florae. We evaluated antibiotic effect by the optical density and the colony test. Results : The optical density and colony test showed that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex of herbs among heat-clearing medicinal had antimircobial effect. Celosiae Semen and Prunellae Spica had antimicrobial susceptibility and selective toxicity in MRSA. Scutellariae Radix and Phellodendri Cortex had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex among heat-clearing medicinal be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

• 대한수의학회지
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• 제57권3호
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• pp.155-158
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• 2017

Surgical castration performed to reduce male-associated problems results in pain and microbial infections in male animals. Therefore, immunocontraception, which is mediated by the animal's own antibodies against reproductive hormones, has been recommended as an alternative to surgical castration when considering the animal's welfare. In this study, a new immunocontraceptive vaccine composed of six tandem copies of gonadotropin-releasing hormone (GnRH) fused to rat granulocyte-macrophage colony-stimulating factor (GM-CSF) was developed, and its efficacy was evaluated in male rats. Three different doses (10, 50, and $100{\mu}g$) of recombinant GM-CSF-GnRH protein were injected three times at intervals of two weeks into male rats. The rats vaccinated with three doses of GM-CSF-GnRH produced a significantly higher level of antibodies against GnRH than that in the negative control rats. Severe atrophy of gonads was observed in rats vaccinated with three doses of GM-CSF-GnRH but not in the negative control rats. The results reveal that the new GnRH vaccine conjugated with rat GM-CSF induces efficient immunocontraception in male rats. This formulation of the immunocontraceptive vaccine would be applicable to both domestic and pet male animals.

• 동아시아식생활학회지
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• 제4권3호
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• pp.123-133
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• 1994

The ground pork patties were made to add two level of sodium chloride(2.5%, 3.0%) and replace part(50%) of the sodium chloride(NaCl) with either potassium chloride(KCl), magnesium chloride(MgCl2) or calcium chloride(CaCl2). These samples were analyzed for their chemical composition, VBN value, TBA value, microbial counts, and cooking loss. The ground pork with NaCl 2.5% was more desirable in saltness than the ground pork with NaCl 3.0%. Replacing 50% of the sodium chloride with potassium chloride was more desirable to flavor, color, juiciness, and overall acceptability than replacing 50% of the sodium chloride with either magnesium chloride or calcium chloride. The ground pork with NaCl 2.5% or NaCl 1.25% ＋KCI 1.60% had higher pH value than the ground pork with NaCl 1.25% ＋MgCl2 0.67% or NaCl 1.25% ＋CaCl2 0.79%. The ground pork with the ground pork with NaCl 2.5% had lower VBN value than the ground pork with either NaCl 1.25%＋KCI 1.60%, NaCl 1.25% ＋ MgCl2 0.67%, or NaCl 1.25% ＋CaCl2 0.79%. The ground pork with NaCl 1.25% ＋ CaCl2 0.67% had higher increase in total colony count than the ground pork with NaCl 2.5% or NaCl 1.25% ＋ CaCl2 0.79%. Cooking loss of ground pork with NaCl 2.5% was lowest and cooking loss of ground pork with NaCl 1.25% ＋ KCl 1.60% was highest. Potassium chloride would not be a substitute for sodium chloride in cooking loss and total colony count but potassium chloride more closely approximated the sensory properties of sodium chloride than either magnesium chloride or calcium chloride.

• 미생물학회지
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• 제47권1호
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• pp.66-73
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• 2011

각 지역에서 수집한 무당벌레(JK, CK, CJ)의 소화기관을 채취하여 장내세균의 밀도를 조사한 결과, 호기배양의 경우 $6.0{\times}10^4$ CFU/gut, 혐기배양 결과 $8.0{\times}10^6$ CFU/gut로 계수되었다. 호기적 조건에서 배양된 세균 집락은 총 7가지 형태로 분류되었으며, 혐기적 조건에서 배양된 집락은 총 3가지 형태로 유사한 특징을 나타내었다. 무당벌레 각 소화기관으로부터 호기성세균 34균주와 혐기성세균 82균주, 총 116균주의 장내세균을 순수분리하였다. 호기성 세균 34균주를 대상으로 16S rRNA 유전자 염기서열을 해석한 결과, ${\alpha}$-Proteobacteria (3균주), ${\gamma}$-Proteobacteria (2 균주), Firmicutes (24 균주), Actinobacteria (4 균주) 그리고 Deinococcus-Thermus (1균주) 계통군으로 분류되었다. Firmicutes 계통군의 Bacillus thuringiensis와 Staphylococcus 속의 다양한 종은 JK, CK 및 CI 무당벌레 소화기관에서 모두 공통적으로 분리되었다. 형태적으로 유사한 혐기세균의 16S rRNA-ARDRA 패턴양상을 분석하여 유사도 70%에서 비교한 결과, 17개 ARDRA group으로 분류되었다. 각 ARDRA group에 속하는 대표 혐기성세균의 16S rRNA 유전자 염기서열을 해석한 결과, 무당벌레 소화기관에서 분리된 모든 혐기성 장내세균은 ${\gamma}$-Proteobacteria 계통군에 속하는 것으로 나타났으며 Hafnia alvei, Enterobacter ludwigii, Enterobacter kobei, Pseudomonas oryzihabitans 그리고 Pseudomonas koreensis와 높은 유연관계를 갖는 것으로 확인되었다. 무당벌레 소화기관으로부터 분리된 전체 장내세균의 약 70%가 ${\gamma}$-Proteobacteria 계통군에 속하였으며, 23%가 Firmicutes 계통군으로 무당벌레 소화기관 내 주요 계통군임이 확인되었다.

• 생태와환경
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• 제38권2호통권112호
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• pp.137-145
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• 2005

전통적인 스크린 방법으로는 자연계에 존재하는 99% 이상의 미생물 자원을 확보하지 못했다. 자연생태계의 핵산을 직접 클로닝하는 전략은 배양 가능한 미생물의 유전적인 정보보다 더 광범위한 유전적인 정보를 전체의 미생물 메타지놈에서 확보하기 위한 계획을 세웠다. 그 결과 유용한 유전자를 탐색하는 한 방법으로 다양한 환경에서 메타지놈 DNA 라이브러리를 구축하는 방법이었다. 본 연구는 국내 중부권에 위치한 대청호로부터 시료를 수집하였고, T-RFLP 방법을 사용하여 미생물 군집의 다양성을 분석하였다. 핵산의 추출은 SDS를 사용한 freeing-thawing 방법을 사용하였으며, 추출한 핵산은 $UltraClean^{TM}kit$ (MoBio, USA)을 사용하여 정제하였다. 메타지놈 라이브러리는 제작은 정제한DNA와 pBACe3.6 vector를 EcoRI, BamHI, 그리고 SacII 등의 제한효소로 partial digestion하였고, 이들을 ligation한 다음 Escherichia coli DH10B에 형질전화 시켜 제작하였다. 메타지놈 라이브러는 14 Mb 정도 확보하였는데, 평균 insert size는 약 13 ${\sim}$ 15 kb이었다. Colony hybridization으로 메타지놈 라이브러리로부터 1, 2-dichloroethane (1, 2-DCE) hydrolytic dehalogenation의 분해에 관련된 유전자를 확인하였다. 1, 2-DCE dehalogenas효소는 기질에 대한 높은 활성을 나타내었다. 1, 2-dichloroethane dehalogenase 유전자의 클론을 만들었고, 염기서열을 분석하였다. 이들 결과로 보아 대청호로부터 제작한 메타지놈에서 dhlA 유전자를 확인한 균주는 1, 2-DCE 분해에 탁월한 능력을 나타내었다.

• 한국축산식품학회지
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• 제35권4호
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• pp.541-550
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• 2015

The purpose of this study was to investigate the effect of lactic acid bacteria (LAB) cell wall extract on the proliferation and cytokine production of immune cells to select suitable probiotics for space food. Ten strains of LAB (Lactobacillus bulgaricus, L. paracasei, L. casei, L. acidophilus, L. plantarum, L. delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pedicoccus pentosaceus) were sub-cultured and further cultured for 3 d to reach 7-10 Log colony-forming units (CFU)/mL prior to cell wall extractions. All LAB cell wall extracts failed to inhibit the proliferation of BALB/c mouse splenocytes or mesenteric lymphocytes. Most LAB cell wall extracts except those of L. plantarum and L. delbrueckii induced the proliferation of both immune cells at tested concentrations. In addition, the production of T_H1 cytokine (IFN-γ) rather than that of T_H2 cytokine (IL-4) was enhanced by LAB cell wall extracts. Of ten LAB extracts, four (from L. acidophilus, L. bulgaricus, L. casei, and S. thermophiles) promoted both cell proliferating and T_H1 cytokine production. These results suggested that these LAB could be used as probiotics to maintain immunity and homeostasis for astronauts in extreme space environment and for general people in normal life.

• 한국식품저장유통학회지
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• 제6권1호
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• pp.43-47
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• 1999

To develop a wrapping film, which suppresses the microbial decay through the storage and distribute of greenhouse fresh produce, the antimicrobial packaging films were made and applied to the preservation of grapes(Campbell early). For the purpose the films were made by adding 1% grapefruit seed extract(GFSE) to LDPE film(Control). Graps were separately wrapped with packaging films in the state of closely-adhered packaging as well as modified atmosphere packaging(MAP). The wrapped grapes were stored at 5$^{\circ}C$ for 65 days and then the colony count of contaminated microorganims, decay ration of grapes, the gas component within the packages and chemical qualities were investigated. The antimicrobial film packaging showed the efficient results to suppress microbial growth as compared with control. The total number of containated microorganisms were decreased gradually through all the storage period. In the closely-adhered packaging and MAP the decay ratios of grapes was 31% and 19%, indivisually. After the storage period of 65 days, the interior gas components of MAP were 4.5% of O2 and 17.6% of CO2, which were efficient for the storage of grapes. In addition, no negative effects in sweetness and acidities occurred.

• Fisheries and Aquatic Sciences
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• 제15권2호
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• pp.91-97
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• 2012

To isolate a nonylphenol (NP)-degrading bacterium, we isolated a single colony from the NP-degrading microbial consortium SW-3, which was previously isolated from an aqueous environment. Ten colonies that exhibited different cell morphologies were isolated and the strains were named SW-3-A, -B, -C, -D, -E, -F1, -F2, -G, -H, and -I. The ability of isolates to degrade NP was evaluated by kinetic analysis by the constant of NP degradation rate ($k_1$) and the half-life time of NP degradation ($t_{1/2}$). SW-3-F1, -F2, -G, and -I strains were superior at degrading NP. The $k_1$ and $t_{1/2}$ values of the four strains were sixfold higher and one-sixth lower, respectively, than those of the consortium strain. Additionally, SW-3-F1, -G, and -I strains were tested for their ability to degrade NP during coculture. NP degradation by coculture with a combination of all three strains was inferior to that of culture conducted with single isolates, suggesting that the three strains are antagonistic toward each other during NP degradation.