• Title/Summary/Keyword: Microbial Community

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Evaluation of Fluoride Removal Effect with Growth of Attached Microbial Community in Middle and Small Stream (중·소하천에서 부착미생물군집의 성장에 따른 불소 제거 효과 평가)

  • Kim, Tae-Kyung;Ryu, Seo-Young;Park, Yoon-A;Lee, Jong-Jun;Joo, Kwang-Jin;Chang, Kwang-Hyeon;Oh, Jong-Min
    • Journal of Environmental Impact Assessment
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    • v.27 no.6
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    • pp.595-603
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    • 2018
  • This study was conducted to understand the growth of attached microbial community in small and medium streams of Gyeonggi-do and the removal efficiency of fluoride with changes in growth. The sites monitoring were carried out for 12 weeks at the P1 and P2(P1 is the point where the discharge water flows. P2 is the downstream point of about 2 km flow), and investigated the water quality and the biomass analysis of the attached microbial community. The growth of the attached microbial community increased up to the 7th investigation, and it was observed that detachment phenomenon occurred. Influencing factors about growth of the attached microbial community were flow rate, velocity, and organic material(T-N & T-P). Meanwhile, fluoride content of attached microbial community also tended to increase until the 7th investigation, and decrease from the 8th. It is assumed that fluoride content has also be reduced with the detachment phenomenon of the attached microbial community. It is expected that this will contribute to the evaluation and management of the use of attached microbial community as a means of stream management. The application of techniques using the attached microbial community should include basic investigation of factors that may affect the growth of the attached microbial community and replacement of the attachment plate according to the time of removal.

T-RFLP Analysis of Microbial Community Structure in Leachate from Landfill Sites (폐기물매립장 침출수내 미생물군집 구조 해석을 위한 T-RFLP의 활용)

  • Yu, Jae-Cheul;Ishigaki, Tomonori;Kamagata, Yoichi;Lee, Tae-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.4
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    • pp.369-378
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    • 2010
  • Microorganisms are key-role player for stabilization of landfill sites. In order to evaluate the availability of T-RFLP(Terminal Restriction Fragment Length Polymorphism) for monitoring microbial community variations during stabilization of landfill sites, the phylogenic diversity of microbial community in the leachate from 4 different full-scale landfills was characterized by T-RFLP based on bacterial 16S rDNA. Main population of microbial community analyzed by T-RFLP was significantly similar with that of microbial community analyzed by clone library analysis. The results of T-RFLP analysis for main population of microbial community in the leachate from landfills with different landfill structures, waste types and landfill ages showed apparently different microbial diversity and structures. Therefore, long-term monitoring of microbial community in leachate from landfill sites by using T-RFLP is expected to be available for evaluation of landfill stability.

Comparison between DNA- and cDNA-based gut microbial community analyses using 16S rRNA gene sequences (16S rRNA 유전자 서열 분석을 이용한 DNA 및 cDNA 기반 장내 미생물 군집 분석의 비교)

  • Jo, Hyejun;Hong, Jiwan;Unno, Tatsuya
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.220-225
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    • 2019
  • Studies based on microbial community analyses have increased in the recent decade since the development of next generation sequencing technology. Associations of gut microbiota with host's health are one of the major outcomes of microbial ecology filed. The major approach for microbial community analysis includes the sequencing of variable regions of 16S rRNA genes, which does not provide the information of bacterial activities. Here, we conducted RNA-based microbial community analysis and compared results obtained from DNA- and its cDNA-based microbial community analyses. Our results indicated that these two approaches differed in the ratio of Firmicutes and Bacteroidetes, known as an obesity indicator, as well as abundance of some key bacteria in gut metabolisms such as butyrate producers and probiotics strains. Therefore, cDNA-based microbial community may provide different insights regarding roles of gut microbiota compared to the previous studies where DNA-based microbial community analyses were performed.

Analysis of the Seasonal Change in Attached Algae and Microbial Community Structure in Sediment Basin Trough of Water Treatment Plant By Using Quinone Profile and PCR-DGGE (Quinone profile과 PCR-DGGE를 이용한 정수장 침전지에서의 부착조류 및 미생물군집의 계절적 변화해석)

  • Yu, Hyun-Sun;Lim, Byung-Ran;Ahn, Kyu-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.3
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    • pp.461-467
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    • 2006
  • The seasonal change in attached algae and microbial community structure at sedimentation basin of water treatment plant was investigated by using quinone profiles and denaturing gel gradient electrophoresis (DGGE). The photosynthetic bacteria and algae contains PQ-9 and VK-1 as major quinone are major component of the total quinone fraction in attached algae and microorganisms on sedimentation basin trough. The microorganisms containing menaquinones appear to be sensitivity to the change in temperature than those containing ubiquinones. The plot of the mole fraction of dominant quinone species ($f_d$) to the DQ values showed higher sensitivity to the seasonal change in the microbial community structure. The results indicated that quinone and DGGE are useful tool for the evaluation of the changes in the microbial community structure.

Characterization of Microbial Community in Biological Wastewater Treatment System Using Respiratory Quinone Profiles

  • Lim Byung-Ran;Ahn Kyu-Hong;Lee Yonghun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2003.05a
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    • pp.111-114
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    • 2003
  • The dynamics of microbial community structure of the various domestic wastewater treatment processes were examined using a novel approach of quinone profiles. The compositions of microbial quinone of 5 sites fer plant and lab-scale activated sludge were analyzed. More than 14 kinds of quinones were observed in the activated sludges tested in this study. The microbial community structure of the plant activated sludge processes a little differed from that of the lab-scale submerged MBR systems. The dominant quinones were UQ-8, UQ-10 followed $MK-8(H_4)$, MK-7 and MK-6. The molar ratio of ubiquinones to menaquinones (UQ/MK) changed from 0.81 to 1.9, indicating that aerobic bacteria dominated the microbial community of the activated sludge examined. The microbial diversity of the activated sludges calculated from the all quinone compositions was 9.5-11.9 and the microbial equability of the activated sludges was 0.64-0.79.

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Microbial Community Structure of Paddy Soil Under Long-term Fertilizer Treatment Using Phospholipid Fatty Acid (PLFA) Analysis

  • Daquiado, Aileen Rose;Kim, Tae Young;Lee, Yong Bok
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.6
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    • pp.474-481
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    • 2013
  • Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available $P_2O_5$ and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.

Effect of Increasing Amounts of Ammonium Nitrogen Induced by Consecutive Mixture of Poultry Manure and Cattle Slurry on the Microbial Community during Thermophilic Anaerobic Digestion

  • Alsouleman, Khulud
    • Journal of Microbiology and Biotechnology
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    • v.29 no.12
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    • pp.1993-2005
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    • 2019
  • Thermophilic anaerobic digestion (TAD) is characterized by higher biogas production rates as a result of assumedly faster microbial metabolic conversion rates compared to mesophilic AD. It was hypothesized that the thermophilic microbiome with its lower diversity than the mesophilic one is more susceptible to disturbances introduced by alterations in the operating factors, as an example, the supply of nitrogen-rich feedstock such as poultry manure (PM). Laboratory scaled TAD experiments using cattle slurry and increasing amounts of PM were carried out to investigate the (in-) stability of the process performance caused by the accumulation of ammonium and ammonia with special emphasis on the microbial community structure and its dynamic variation. The results revealed that the moderate PM addition, i.e., 25% (vol/vol based on volatile substances) PM, resulted in a reorganization of the microbial community structure which was still working sufficiently. With 50% PM application, the microbial community was further stepwise re-organized and was able to compensate for the high cytotoxic ammonia contents only for a short time resulting in consequent process disturbance and final process failure. This study demonstrated the ability of the acclimated thermophilic microbial community to tolerate a certain amount of nitrogen-rich substrate.

Influence of FA and FNA to Microbial Community in Sequencing Batch Ammonium Partial Nitrification System (암모니아 부분산화 공정의 제어와 미생물 군집의 변화)

  • Ahn, Johwan
    • Journal of Korean Society on Water Environment
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    • v.31 no.4
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    • pp.399-406
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    • 2015
  • A sequencing batch reactor was operated under different pH conditions to see the influence of free ammonia (FA) and free nitrous acid (FNA) to microbial community on ammonium partial nitrification. Long-term influences of FA and FNA were evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis and fluorescence in situ hybridization. Nitrite accumulation was successfully achieved at pH 8.2 and 6.3. The shifts in the microbial community were observed when influent ammonia concentration increased to 1 g $NH_4$-N/L at pH 8.2, and then when pH was dropped to 6.3. Both Nitrosomonas and Nitrosospira were selected during the startup of the reactor, and eventually became dominant members as ammonia-oxidizing bacteria. The results of molecular microbiological analysis strongly suggested that the composition of microbial community was changed according to the method used to control nitrite-oxidizing bacteria.

Comparison of Microbial Community of Rhizosphere and Endosphere in Kiwifruit

  • Kim, Min-Jung;Do, Heeil;Cho, Gyeongjun;Jeong, Rae-Dong;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • v.35 no.6
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    • pp.705-711
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    • 2019
  • Understanding the microbial community and function are crucial knowledge for crop management. In this study, bacterial and fungal community structures both rhizosphere and endosphere in kiwifruit were analyzed to gain our knowledge in kiwifruit microbiome. Microbial community in rhizosphere was less variation than endosphere community. Functional prediction results demonstrated that abundance of saprotrophic fungi was similar in both rhizosphere and endosphere, but potential pathogenic fungi was more abundance in endosphere than in rhizosphere. This finding suggested that maintain healthy soil is the first priority to protect the host plant against biotic stresses.

Bioinformatic Suggestions on MiSeq-Based Microbial Community Analysis

  • Unno, Tatsuya
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.765-770
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    • 2015
  • Recent sequencing technology development has revolutionized fields of microbial ecology. MiSeq-based microbial community analysis allows us to sequence more than a few hundred samples at a time, which is far more cost-effective than pyrosequencing. The approach, however, has not been preferably used owing to computational difficulties of processing huge amounts of data as well as known Illumina-derived artefact problems with amplicon sequencing. The choice of assembly software to take advantage of paired-end sequencing and methods to remove Illumina artefacts sequences are discussed. The protocol we suggest not only removed erroneous reads, but also dramatically reduced computational workload, which allows even a typical desktop computer to process a huge amount of sequence data generated with Illumina sequencers. We also developed a Web interface (http://biotech.jejunu.ac.kr/ ~abl/16s/) that allows users to conduct fastq-merging and mothur batch creation. The study presented here should provide technical advantages and supports in applying MiSeq-based microbial community analysis.