• Title/Summary/Keyword: Micro-environmental analysis

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Cross-talk between Phosphate Starvation and Other Environmental Stress Signaling Pathways in Plants

  • Baek, Dongwon;Chun, Hyun Jin;Yun, Dae-Jin;Kim, Min Chul
    • Molecules and Cells
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    • v.40 no.10
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    • pp.697-705
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    • 2017
  • The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.

Particle Spacing Analysis of Frozen Sand Specimens with Various Fine Contents by Micro X-ray Computed Tomography Scanning (Micro X-ray CT 촬영을 통한 동결 사질토 시료의 세립분 함유량에 따른 입자간 거리 분석)

  • Chae, Deokho;Lee, Jangguen;Kim, Kwang-Yeom;Cho, Wanjei
    • Journal of the Korean GEO-environmental Society
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    • v.18 no.1
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    • pp.31-35
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    • 2017
  • The mechanical characteristics of frozen sand greatly depend on the frozen temperature and the fine contents according to the previous study by Chae et al. (2015). There are two hypotheses to explain this experimental results; one is the unfrozen water contents greatly affected by the fine contents and frozen temperature and the other is the sand particle spacing greatly affected by the pore-ice. To evaluate the latter hypothesis, the micro X-ray CT scan was performed. The micro X-ray CT scanning, one of the actively performed interdisciplinary research area, has a high resolution with micrometer unit allows to investigate internal structure of soils. In this study, X-ray CT technique was applied to investigate the effect of the frozen temperature and fine contents on the sand particle minimum and average spacing with the developed image processing techniques. Based on the spacing analysis, the frozen temperature and fine contents have little influence on the sand particle spacing in the frozen sand specimens.

Evaluation of micro-channel characteristics of fused silica glass using powder blasting (Powder blasting을 이용한 Fused silica glass의 마이크로 채널 가공 및 특성 평가에 관한 연구)

  • Lee, Jung-Won;Kim, Tae-Min;Shin, Bong-Cheol
    • Design & Manufacturing
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    • v.14 no.1
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    • pp.36-41
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    • 2020
  • Recently, due to the development of MEMS technology, researches for the production of effective micro structures and shapes have been actively conducted. However, the process technology based on chemical etching has a number of problems such as environmental pollution and time problems due to multi-process. Various processes to cope with this process are being studied, and one of the mechanical etching processes is the powder blasting process. This process is a method of spraying fine particles, which has the advantage of being an effective process in manufacturing hard brittle materials. However, it is also a process that adversely affects the material surface roughness and material properties due to the impact of the injection of fine particles. In this study, after fabricating micro-channels in fused silica glass with excellent optical properties among the hard brittle materials, we used the nano indentation system to analyze the micro parts using nano-particles as well as machinability and surface roughness analysis of the processed surface. The analysis was performed for the effective processing of powder blasting.

Development of Application Block Using Geobond and Ash from Sewage Sludge Incinerator II (하수슬러지 소각재와 무기바인더를 이용한 응용 블록 개발 II)

  • Lee, Hyun-joo
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.7
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    • pp.412-417
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    • 2015
  • This study investigated to recycle geobond and ash produced in thesewage sludge incinerator using reduction/stabilization. Nonsintering process was performed by binding cement (High Early Strength Portland cement, Micro cement), geobond and sand mixed with sewage sludge ash (SSA). Chemical ingradients of the sewage sludge ash was mainly composed of $SiO_2$, $Al_2O_3$, $Fe_2O_3$, CaO and others, which were similar to those of the each binders consisting High Early Strength Portland cement, Micro cement and geobond. Results showed that unconfined the long term compressive strength could be obtained components of sewage sludge ash. It exceeded more than double score 64.6 MPa of the Korean standard ($22.54MPa=229.7kg/cm^2$). Microstructure of solidified block for the different admixture was related to the compressive strength according to SEM analysis. Optimum mixing range of the sewage sludge ash to each binders were found to be 10~40% which can widly safely regulate the confined a long term compressive strength. The best binder of long term compressive strengh was revealed Geobond more than High Early Strength Portland cement and Micro cement. This study revealed the sewage sludge ash can be partial replacement of the inorganic binder & application block for recycling.

Infection Properties of Oak Wilt Disease in Bukhansan National Park Adjacent to Metropolitan Areas in Korea

  • Choi, Jin-Woo;Yeum, Jung-Hun
    • Journal of Environmental Science International
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    • v.26 no.7
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    • pp.803-815
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    • 2017
  • In this study of the oak wilt disease that has occurred in a large scale in a protected area located near South Korea's metropolitan region, a detailed analysis has been conducted on the terrain, species and Diameters at Breast Height (DBH) of infected trees to identify the distribution of infection properties in the affected area. Taking into consideration the distribution of oak tree vegetation, a total of 4,640 quadrats in a size of 10 m by 10 m, have been set; and oak tree species, the DBH and infection damage per quadrat have been investigated. Geological properties have been analyzed according to elevation, slope, aspect and micro topography while a weighted value has been given according to the degree of infection in order to calculate an infection index. Through correlation analysis, the infection ratio of seriously-damaged and withered trees and the infection index have been analyzed with regards to the geological properties, tree species and DBH. The analysis shows that the disease tends to affect an area with medium elevation rather than those in the highest or lowest areas and that serious damage has been observed at rugged spots with a steep gradient (more than $30^{\circ}$). Although there has been no distinct tendency with regards to aspect, the infection ratio is relatively high in areas facing the north while the seriously-damaged and withered ratio are high in areas facing the south. In terms of micro topography, more damage has been spotted in valley terrain. Quercus mongolica has sustained more damage than other species. When it comes to the DBH, as seen in previous studies, large trees have suffered severe damage, but the analysis has also revealed conspicuous damage to medium trees with a DBH of 15-20 cm, which had not previously been considered at high risk.

Bio-oil production using residual sewage sludge after lipid and carbohydrate extraction

  • Supaporn, Pansuwan;Ly, Hoang Vu;Kim, Seung-Soo;Yeom, Sung Ho
    • Environmental Engineering Research
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    • v.24 no.2
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    • pp.202-210
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    • 2019
  • In order to maximize the utilization of sewage sludge, a waste from wastewater treatment facility, the residual sewage sludge generated after lipid and carbohydrate extraction for biodiesel and bioethanol production was used to produce bio-oil by pyrolysis. Thermogravimetric analysis showed that sludge pyrolysis mainly occurred between 200 and $550^{\circ}C$ (with peaks formed around 337.0 and $379.3^{\circ}C$) with the decomposition of the main components (carbohydrate, lipid, and protein). Bio-oil was produced using a micro-tubing reactor, and its yield (wt%, g-bio-oil/g-residual sewage sludge) increased with an increase in the reaction temperature and time. The maximum bio-oil yield of 33.3% was obtained after pyrolysis at $390^{\circ}C$ for 5 min, where the largest amount of energy was introduced into the reactor to break the bonds of organic compounds in the sludge. The main components of bio-oil were found to be trans-2-pentenoic acid and 2-methyl-2-pentenoic acid with the highest selectivity of 28.4% and 12.3%, respectively. The kinetic rate constants indicated that the predominant reaction pathway was sewage sludge to bio-oil ($0.1054min^{-1}$), and subsequently to gas ($0.0541min^{-1}$), rather than the direct conversion of sewage sludge to gas ($0.0318min^{-1}$).

RC deep beams with unconventional geometries: Experimental and numerical analyses

  • Vieira, Agno Alves;Melo, Guilherme Sales S.A.;Miranda, Antonio C.O.
    • Computers and Concrete
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    • v.26 no.4
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    • pp.351-365
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    • 2020
  • This work presents numerical and experimental analyses of the behavior of reinforced-concrete deep beams with unconventional geometries. The main goal here is to experimentally and numerically study these geometries to find possible new behaviors due to the material nonlinearity of reinforced concrete with complex geometries. Usually, unconventional geometries result from innovative designs; in general, studies of reinforced concrete structures are performed only on conventional members such as beams, columns, and labs. To achieve the goal, four reinforced-concrete deep beams with geometries not addressed in the literature were tested. The models were numerically analyzed with the Adaptive Micro Truss Model (AMTM), which is the proposed method, to address new geometries. This work also studied the main parameters of the constitutive model of concrete based on a statistical analysis of the finite element (FE) results. To estimate the ultimate loads, FE simulations were performed using the Monte Carlo method. Based on the obtained ultimate loads, a probabilistic distribution was created, and the final ultimate loads were computed.

Estimation of micro-biota in the Upo wetland using eukaryotic barcode molecular markers

  • Park, Hyun-Chul;Bae, Chang-Hwan;Jun, Ju-Min;Kwak, Myoung-Hai
    • Journal of Ecology and Environment
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    • v.34 no.3
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    • pp.323-331
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    • 2011
  • Biodiversity and the community composition of micro-eukaryotic organisms were investigated in the Upo wetland in Korea using molecular analysis. Molecular identification was performed using cytochrome oxidase I (COI) and small subunit ribosomal DNA (SSU rDNA). The genomic DNA was isolated directly from soil samples. The COI and SSU rDNA regions were amplified using universal primers and then sequenced after cloning. In a similarity search of the obtained sequences with BLAST in the Genbank database, the closely related sequences from NCBI were used to identify the amplified sequences. A total of six eukaryotic groups (Annelida, Arthropoda, Rotifera, Chlorophyta, Bacillariophyta, and Stramenopiles) with COI and six groups (Annelida, Arthropoda, Rotifera, Alveolata, Fungi, and Apicomplexa) with SSU rDNA genes were determined in the Upo wetland. Among 38 taxa in 20 genera, which are closely related to the amplified sequences, 10 genera (50%) were newly reported in Korea and five genera (25%) were shown to be distributed in the Upo wetland. This approach is applicable to the development of an efficient method for monitoring biodiversity without traditional taxonomic processes and is expected to produce more accurate results in depositing molecular barcode data in the near future.

Micro and Nano Engineered High Volume Ultrafine Fly Ash Cement Composite with and without Additives

  • Roychand, R.;De Silva, S.;Law, D.;Setunge, S.
    • International Journal of Concrete Structures and Materials
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    • v.10 no.1
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    • pp.113-124
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    • 2016
  • This paper presents the effect of silica fume and nano silica, used individually and in combination with the set accelerator and/or hydrated lime, on the properties of class F high volume ultra fine fly ash (HV-UFFA) cement composites, replacing 80 % of cement (OPC). Compressive strength test along with thermogravimetric analysis, X-ray diffraction and scanning electron microscopy were undertaken to study the effect of various elements on the physico-chemical behaviour of the blended composites. The results show that silica fume when used in combination with the set accelerator and hydrated lime in HV-UFFA cement mortar, improves its 7 and 28 day strength by 273 and 413 %, respectively, compared to the binary blended cement fly ash mortar. On the contrary, when nano silica is used in combination with set accelerator and hydrated lime in HV-UFFA cement mortar, the disjoining pressure in conjunction with the self-desiccation effect induces high early age micro cracking, resulting in hindering the development of compressive strength. However, when nano silica is used without the additives, it improves the 7 and 28 day strengths of HV-UFFA cement mortar by 918 and 567 %, respectively and the compressive strengths are comparable to that of OPC.

Analysis of Microcystins(LR, YR and RR) in Water (Micro-HPLC/IS/MS에 의한 수 중의 Microcystins (LR, YR 및 RR)의 분석)

  • Park, Kwan Su;Bae, Jun Hyun;Kang, Jun Gil;Kim, Youn Doo
    • Journal of the Korean Chemical Society
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    • v.45 no.6
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    • pp.524-531
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    • 2001
  • Micro-HPLC/IS/MS after solid phase extraction has been employed to enhance the accu-racy in the determination of toxic microcystins, such as microcystin-LR, -YR and -RR. The absorbance at 238 nm in HPLC/UV and characteristic spectra of 135 m/z and $[M+H]^+$ m/z in MS have been widely monitored to identify those microcystin-LR, -YR and -RR. In this study, new lines at 507 m/z for LR, 520 m/z for RR and 532 m/z for YR have been additionally detected in the micro-HPLC/IS/MS spectrum, corresponding to double charge. The micro-HPLC/IS/MS methodology has been applied to investigate the presence of the toxic microcystins in Taecheong lake.

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