• 생명과학회지
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• 제33권2호
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• pp.169-175
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• 2023

자외선으로 야기되는 피부조직의 손상에 대한 방어기전으로 멜라닌세포는 멜라닌 색소를 생산한다. 이러한 멜라닌 생성에는 tyrosinase 효소가 중추적인 역할을 수행한다. 그러나 과다한 멜라닌 생성은 피부조직에 주근깨와 검버섯 등의 과다 색소침착 병변을 일으킨다. 따라서 tyrosinase 발현이나 활성을 조절함으로써, 과도한 멜라닌 생성을 억제하는 물질을 발굴하는 것은 임상적인 의미가 있다. Catechin은 tyrosinase 발현을 저해함으로써 멜라닌 생성을 억제하는 화합물로 알려져 있다. 본 연구에서는 B16F10 마우스 흑색종 세포주와 버섯에서 추출된 tyrosinase를 사용하여, 참느릅나무의 줄기 껍질에서 추출 및 동정된 catechin 유도체 화합물(C5A, C7A, C7G, 및 C7X)의 멜라닌 생성 억제 효능을 검증하였다. 먼저, B16F10 세포주에서, 세포독성을 나타내지 않는 농도의 catechin 유도체 화합물 처리는, 모두α-MSH로 촉진된 멜라닌 생합성을 억제하는 효능을 보였다. Catechin 유도체 화합물의 이러한 효능은 멜라닌 생합성 저해제로 잘 알려진 kojic acid와 비슷한 수준이였다. 또한, C5A와 C7A는 버섯에서 추출된 tyrosinase의 효소 활성을 통계적으로 유의하게 억제하였다. 또한, 컴퓨터 분석을 통해 C5A와 C7A는 kojic acid와 유사한 수준으로 tyrosinase의 active site에 결합할 것으로 예측되었다. 이에 더하여, 4종류의 catechin 유도체 화합물 모두가 α-MSH 처리된 B16F10 세포주에서 tyrosinase 단백질 발현을 감소시켰다. 본 연구결과를 종합하면, 참느릅나무에서 추출/동정된 catechin 유도체 화합물은 멜라닌세포의 멜라닌 생성을 효과적으로 억제하였다. 따라서, 본 연구결과는 이러한 catechin 유도체 화합물이 피부의 과다 색소침착 질환 제어를 위한 새로운 조절 물질로 응용될 수 있음을 제시한다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.794-798
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• 2003

천연추출물로서 미백 재료로 널리 쓰이고 있는 옥용산에 대해서 in vitro 미백활성을 UV 흡수능, tyrosinase 저해활성 그리고 free radical 소거활성을 측정함으로서 검정해 보았다. 옥용산 UV-B와 C 영역에 걸쳐서 높은 자외선 흡수능을 보이는 것으로 관찰이 되었으며 0.8 % 이상의 농도에서 80% 이상의 tyrosinase 저해활성을 가지는 것을 관찰하였다. 또한 조협, 승마, 감송, 정향, 찹쌀분과 옥용산의 추출물은 높은 free radical 소거능을 가지는 것으로 확인되었다.

• 대한세포병리학회지
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• 제9권1호
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• pp.111-115
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• 1998

Although the characteristic cytologic features of melanoma have been well described the diagnosis of metastatic melanoma by fine needle aspiration cytology (FNAC) may be difficult in the case of amelanotic melanoma and in the absence of awareness of clinical history. Furthermore, when the breast is the site of initial presentation, it could simulate a primary breast carcinoma leading to misdiagnosis. The recognition of metastatic malignant melanoma in FNAC material is essential to avoid an unnecessary mastectomy and to ensure appropriate chemotherapy. We experienced a case of metastatic melanoma of breast which presented as solitary breast mass in a 56-year-old woman. She had a history of surgical excision of right foot for melanoma one year ago. The cytologic smears were composed of noncohesive epithelioid cells with round or eccentric nuclei, bi-or multi-nucleation, prominent nucleoli, fine chromatin, and intranuclear inclusions. The cytoplasm of tumor cells had scanty melanin pigment but were diffusely positive for S-100 protein.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.204-208
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• 2002

A Streptomyces sp. isolated from a soil sample collected in Taejeon, Korea has previously been found to produce two new polyene antibiotics. The two new antibiotics were named "16-methyloxazolomycin (antibacterial)" and "tetrin C (antifungal)", and their chemical structures are presented elsewhere [10, 11]. In the current study, chemotaxonomy, numerical taxonomy, and ISP methods were all employed for the taxonomic study. The spore chains were spirales and the spore surface was smooth. The spore mass was a gray series and no melanin pigment was produced. On the basis of the morphological and physiological properties, the microorganism was identified to be Streptomyces erumpens, belonging to the gray series of category IV, as defined by Bergey′s Manual. Tetrin C at the concentration of 20 ${\mu}g$/ml demonstrated a potent in vivo (pot test) preventive effect against rice blast, rice sheath blight, cucumber gray mold, wheat powdery mildew, and barley leaf rust.

• 생약학회지
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• 제36권4호통권143호
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• pp.273-277
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• 2005

In mammalian melanocytes, melanin synthesis is controlled by tyrosinase, the key enzyme in the pigment synthesis. In this study, to develop a new whitening agent, we have investigated the antioxidant and the inhibitory effect of Artemisia anomala extract on tyrosinase activity and melanigenesis in the B16/F1 melanoma cells. The inhibition ratio of tyrosinase activity of butanol fraction from A. anomala was higher than that of arbutin ($97.5{\pm}0.5%$ at the concentration of 2 mg/ml). The butanol fraction was shown scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals in a dose dependent manner. The highest inhibitory activity of melanogenesis was also butanol fraction ($25.0{\pm}3%$ at the concentration of $200\;{\mu}g/ml$). From these results, we suggest that the A. anomala extract might be used to be a potential agent for skin whitening.

• Applied Microscopy
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• 제18권1호
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• pp.49-59
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• 1988

Fine structures of the testis and vas deferens in the fall-web worm, Hyphantria cunea Drury, are studied with electron microscope. Adult worms have single testis close to the midlines of the abdomen. Testis is composed of 4 follicles which are incompletly separated from each other and bounded together by a peritoneal sheath. The peritoneal sheath consisted of outer cuticular layer and two kinds of inner layers, in which glycogen particles are dispersed commonly. These two layers are divided by the morphology of cytoplasmic granules. Follicular epithelium forming the wall of the follicles have melanin pigment granules, and trachea or tracheoles are extended through this epithelium. In the cysts of adult testis, matured spermatozoa are grouped together in bundles and after releasing the sperm bundles to the vas deferens, lamellar shaped lysosomes appeared in the cytoplasms of the cyst cells. The number of spermatozoa per cyst is exactly 256 ($2^8$), this number is characteristics of the Lepidoperan species. Vas deferens is a tube with a fairly thick bounding epithelium, a basement membrane and a layer of circular muscle outside it. At the apical portion of the epithelial cells, microvilli are well developed. And in the cytoplasms of these cells, numerous excretory granules are observed.

• Journal of Periodontal and Implant Science
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• 제41권4호
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• pp.201-207
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• 2011

Purpose: The aim of this study is to compare two different gingival depigmentation techniques using an erbium:yttrium-aluminum-garnet (Er:YAG) laser and rotary instruments. Methods: Two patients with melanin pigmentation of gingiva were treated with different gingival depigmentation techniques. Ablation of the gingiva by Er:YAG laser was performed on the right side, and abrasion with a rotary round bur on the opposite side. Results: The patients were satisfied with the esthetically significant improvement with each method. However, some pigment still remained on the marginal gingival and papilla. The visual analog scale did not yield much difference between the two methods, with slightly more pain on the Er:YAG laser treated site. Conclusions: The results of these cases suggest that ablation of the gingiva by an Er:YAG laser and abrasion with a rotary round bur is good enough to achieve esthetic satisfaction and fair wound healing without infection or severe pain. Prudent care about the gingival condition, such as the gingival thickness and degree of pigmentation along with appropriate assessment is needed in ablation by the Er:YAG laser procedure.

• 대한수의학회지
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• 제45권3호
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• pp.335-339
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• 2005

The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo, we performed a modified PCR-RFLP analysis of MC1R gene using single nucleotide polymorphism (SNP) within MC1R as a target. A size of 538 bp (537 bp for Hanwoo) was amplified by PCR, digested with Hpa II, and electrophoresed on a 1.5% agarose gel. A PCR product from Hanwoo showed a single band of 537 bp, whereas two fragments of 328 bp and 210 bp were detected in both Holstein and Black angus. The current result suggests that the PCR-RFLP using our primers and enzyme digestion system would be very accurate, easy and reproducible method to discriminate between Hanwoo and Holstein/Black angus meat.

• 대한수의학회지
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• 제45권1호
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• pp.25-28
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• 2005

The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo meat, we performed a single nucleotide polymorphism (SNP) analysis in Melanocortin 1 receptor (MC1R) gene using TaqMan$^{(R)}$ MGB probe-based real-time PCR. Two specific probes (one for Hanwoo and the other for Holstein and Black angus) were designed. At the 5' end of 2 TaqMan$^{(R)}$ MGB probes, 6-carboxyfluorescein (FAM) was labeled for Hanwoo, and VIC for Holstein and Black angus. As a result, Hanwoo samples showed FAM-positive signal only, whereas other samples showed VIC-positive. This result suggests that the TaqMan$^{(R)}$ MGB probe based real-time PCR technique would be very accurate, easy and reproducible method to discriminate between Hanwoo meat and Holstein/Black angus meat.

• 미생물학회지
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• 제42권3호
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• pp.235-237
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• 2006

먹물버섯은 버섯 시원체로부터 버섯이 성숙되는 과정에서 자가소화가 일어나 먹물이라 불리는 검은 액체를 생성한다. 이 과정에서 멜라닌을 생성하는 laccase, 균류 세포벽 성분의 하나인 키틴을 분해하는 chitinase의 관련을 분석하고자 Northern hybridization 방법을 이용하여 유전자의 발현을 분석하였다. 시원체가 생성되고 버섯이 성숙되어 먹물을 생성하는 시기에 따라 멜라닌색소 생성 효소인 laccase와 킨틴분해효소인 chitinase의 발현이 증가하는 것이 확인되었다.