Browse > Article

Identification of Streptomyces sp. Producing New Polyene Antibiotics and In Vivo Antimicrobial Activity of Tetrin C Against Phytopathogenic Fungi  

CHOI, WON-CHANG (Department of Clinical Pathology, Hyechun College)
SEOK-YEON HWANG (Department of Clinical Pathology, Chungbuk National University Hospital)
TAE-KYU PARK (Department of Life Science, College of Natural Science, Konkuk University)
SI-KWAN KIM (Department of Life Science, College of Natural Science, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.2, 2002 , pp. 204-208 More about this Journal
Abstract
A Streptomyces sp. isolated from a soil sample collected in Taejeon, Korea has previously been found to produce two new polyene antibiotics. The two new antibiotics were named "16-methyloxazolomycin (antibacterial)" and "tetrin C (antifungal)", and their chemical structures are presented elsewhere [10, 11]. In the current study, chemotaxonomy, numerical taxonomy, and ISP methods were all employed for the taxonomic study. The spore chains were spirales and the spore surface was smooth. The spore mass was a gray series and no melanin pigment was produced. On the basis of the morphological and physiological properties, the microorganism was identified to be Streptomyces erumpens, belonging to the gray series of category IV, as defined by Bergey′s Manual. Tetrin C at the concentration of 20 ${\mu}g$/ml demonstrated a potent in vivo (pot test) preventive effect against rice blast, rice sheath blight, cucumber gray mold, wheat powdery mildew, and barley leaf rust.
Keywords
Streptomyces erumpens; taxonomy; tetrin C; phytopathogenic fungi; preventive effect;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 10  (Related Records In Web of Science)
연도 인용수 순위
1 Harper, J. J. and G. H. G. Davis. 1979. Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. International J. Systematic Bacteriol. 29: 56-58
2 ISCC-NBS Color-Name Charts Illustrated with Centroid Colors. National Bureau of Standards, U.S.A
3 Ryu, G. S., S. J. Hwang, and S. K. Kim. 1997. 16- Methyloxazolomycin, a new antimicrobial and cytotoxic substance produced by a Streptomyces sp. J. Antibiotics 50: 1064-1066
4 Shirling, E. B. and D. Gottlieb. 1968. Cooperative description of type cultures of Streptomyces. II. Species description from first study. International J. Systematic Bacteriol. 18: 69- 189
5 Shirling, E. B. and D. Gottlieb. 1968. Cooperative description of type cultures of Streptomyces. IV. Species description from the second, third and fourth studies. International J. Systematic Bacteriol. 19: 391-512
6 Waksman, S. A. 1959. Strain specificity and production of antibiotic substances. Proc. Nat. Acad. Sci. USA 45: 1043- 1047   DOI   ScienceOn
7 Williams, S. T., M. E. Sharpe, and J. G. Holt. 1989. Bergey’s Manual of Systematic Bacteriology (Vol. 4). Williams & Wilkins, Baltimore, U.S.A
8 Williams, S. T., M. Goodfellow, G. Alderson, E. M. H. Wellington, P. H. A. Sneath, and M. J. Sackin. 1983. Numerical classification of Streptomyces and related genera. J. General Microbiol. 129: 1743-1813
9 Ogura, M., H. Nakayama, K. Furihata, A. Shimatsu, H. Seto, and N. Otake. 1985. Isolation and structural determination of a new antibiotic curromycin B. Agri. Biol. Chem. 49: 1909- 1910
10 Ogura, M., H. Nakayama, K. Furihata, H. Seto, and N. Otake. 1985. Structure of a new antibiotic curromycin A produced by a genetically modified strain of Streptomyces hygroscopicus, a polyether antibiotic producing organism. J. Antibiotics 38: 669-673
11 Shirling, E. B. and D. Gottlieb. 1972. Cooperative description of type strains of Streptomyces. V. Additional descriptions. International J. Systematic Bacteriol. 22: 265-394
12 Calot, L. and A. P. Cercos. 1963. Streptomyces ornatus, nov. sp. et Streptomyces erumpens, nov. sp. producteurs dornamicine et antibiotique 117732. Ann. Inst. Pasteur (Paris) 105: 159-161
13 Williams, S. T., M. Goodfellow, E. M. H. Wellington, J. C. Vickers, G. Alderson, P. H. A. Sneath, and M. J. Sackin. 1983. A probability matrix for identification of some Streptomyces. J. General Microbiol. 129: 1815-1830
14 Ryu, G. S., W. C. Choi, S. J. Hwang, W. H. Yeo, C. S. Lee, and S. K. Kim. 1999. Tetrin C, a new glycosylated polyene macrolide antibiotic produced by Streptomyces sp. GK9244. J. Nat. Prod. 62: 917-919
15 Goodfellow, M. and G. Alderson. 1979. Numerical taxonomy of Actinomadura and related actinomycetes. J. General Microbiol. 112: 95-111   DOI
16 Shirling, E. B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. International J. Systematic Bacteriol. 16: 313-340
17 Hsu, S. C. and J. L. Lockwood. 1975. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied Microbiol. 29: 422-426
18 Mori, T., K. Takahashi, M. Kashiwabara, D. Uemura, C. Katayama, S. Iwadare, Y. Shizuri, R. Mitomo, F. Nakano, and A. Matsuzaki. 1985. Structure of oxazolomycin, a novel blactone antibiotic. Tetrahedron Lett. 26: 1073-1076
19 Shirling, E. B. and D. Gottlieb. 1968. Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. International J. Systematic Bacteriol. 18: 279-392
20 Kim, S. K., S. S. Kim, and C. H. Kim. 1990. New antibiotics produced by Streptomyces melanosporofaciens. I. Taxonomy of the producing microorganism. Kor. J. Appl. Microbiol. Biotechnol. 18: 624-632