• Plant Resources
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• v.8 no.2
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• pp.116-121
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• 2005

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous or methanol extracts and plant residues from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine its allelopathic effects, and the results showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Aqueous leaf extracts of $40g\;L^{-1}$ were completely inhibitory on root growth of alfalfa, while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Leaf residue incorporation at $100g\;kg^{-1}$ into soil on seedling growth of barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) inhibited both shoot and root fresh weights of barnyard grass by 94 and $96\%$, respectively. Methanol extracts from BuOn and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did those from hexane and water fractions. The results based on bioassay of extracts and residues show that X. occidentale had potent an allelopathic activity against other plant species.

• Plant Resources
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• v.7 no.1
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• pp.47-53
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• 2004

Several wild plant species are known to contain biologically active substances that are allelopathic to weed species as well as antioxidant to foods. Plant extracts or residues from leaves of 4 species, Achyranthes japonica (speedwell), Cucumis sativus (Cucumber), Trifolium repens (white clover), and Vicia angustifolia (narrowleaf vetch) were bioassayed against Medicago sativa (alfalfa) or Echinochloa crus-galli (barnyard grass) to determine their allelopathic effects, and used for measurement of antioxidant activities. The aqueous extracts applied on filter paper significantly inhibited root growth of alfalfa. Aqueous extracts or residues from V. angustifolia showed the most inhibitory effect on alfalfa or barnyard grass seedling growth and followed by A. japonica and T. repens. Oxidative stability by Rancimat method, antioxidant activity by TBA (2-thiobarbituric acid) method and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity for the ground samples or methanol extracts were the greatest in V. angustifolia, although were less than those of commonly used antioxidants, BHT (butylated hydroxytoluene) and ascorbic acid. These results suggest that the wild plant species had potent allelopathic and antioxidant activities, and that their activities differed depending on plant species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.4
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• pp.298-302
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• 2019

Acidic soil significantly reduces crop productivity mainly due to aluminum (Al) toxicity. Alfalfa (Medicago sativa L.) roots were exposed to aluminum stress (Al₃⁺) in calcium chloride (CaCl₂) solution (pH4.5) and root growth, physiological and antioxidant enzyme responses were investigated. The root growth (length) was significantly inhibited after 48 h of aluminum stress imposition. Histochemical staining with hematoxylin indicated significant accumulation of aluminum in Al stress-treated root tissues. Histochemical assay were also performed to detect superoxide anion, hydrogen peroxide and lipid peroxidation, which were found to be more in root tissues treated with higher aluminum concentrations. The enzymatic activity of CAT, POD and GR in root tissues was slightly increased after Al stress treatment. The result suggests that Al stress alters root growth in alfalfa and induces reactive oxygen species (ROS) production, and demonstrates that antioxidant enzymes involved in detoxification of Al-mediated oxidative stress.

• The Korean Journal of Ecology
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• v.27 no.1
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• pp.43-47
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• 2004

This research was investigated to prepare basic data in a study on the copper tolerance of herbaceous plants through the growth rate and the elimination rate dependent on Cu concentration of 6 species; Commelina communis, Medicago sativa, Echinochloa frumentancea, Zea mays, Helianthus annuus and Abutilon avicennae, We examined the germination rate, root and shoot growth of seedling and fresh biomass of 6 species (Commelina communis, Medicago sativa, Echinochloa frumentancea, Zea mays, Helianthus annuus and Abutilon avicennae) painted to Cu contaminated soil (50, 100, 200, 300-CuCl₂/㎏) and control for 14 days. The germination rate of H. annuus, E. frumentancea and C. communis were not affected by Cu concentration. However, root and shoot growth of H. annuus was about 7% of control and the biomass was 35% of control at 300 ㎎-CuCl₂/㎏. E. frumentancea and C. communis that showed good growth rate at higher Cu contaminated soil (>200 -CuCl₂/㎏), were the most tolerant plant to Cu concentration. Especially, E. frumentancea eliminated over 30% of Cu in soil and the amount of Cu uptake increased with increasing Cu concentration; 1,020㎎ Cu per 1 ㎏ of soil at 300 ㎎-CuCl₂/㎏. From these results, we concluded that E. frumentancea would be used for phytoremediation.

• Korean Journal of Weed Science
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• v.15 no.2
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• pp.121-130
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• 1995

Greenhouse and laboratory studies were conducted to investigate allelopathic potential of some weed species on alfalfa(Medicago sativa L.) germination and seedling growth. In the comparison between top(leaves+stems) and root extracts, top extract exhibited greater allelopathic effects on alfalfa germination than that of root. The various weed species extract differently responded to alfalfa test species, WL-320, in terms of allelopathic effect. Top and root aqueous extracts of lambsquarter(Chenopodium album L.), giant foxtail(Setaria faberii Herrm.), redroot pigweed (Amaranthus retroflexus L.), velvetleaf(Abutilon theophrasti Medic.), crabgrass(Digitaria sanguinalis L.), canada thistle(Cirsium arvense L.) and prostrate knotweed(Polygonium aviculare L.) significantly inhibited germination, seedling length, weight, vigor, and rate of germination of alfalfa. The regression slopes of various top extracts showed that velvetleaf(b=3.69) extracts were the most inhibitory, while large crabgrass(b=2.39) extracts had the least allelopathic effect on alfalfa germination. Germination, seedling length and weight of alfalfa were inversely proportional to the concentration of dried velvetleaf extracts. Also, more of the toxic effects were observed from the dried extracts compared to the fresh extracts. Residue of velvetleaf inhibited significantly alfalfa emergence and survival percentage compared to the control. The emergence and survival percentage of alfalfa were 44%, 57% at 1.0% residue treatment, respectively. When weed residues were mixed with silica sand with incubation time, velvetleaf residue most inhibited alfalfa growth. The degree of inhibition increased as incubation time increased. An incubation for 72h caused the greatest inhibition of alfalfa growth. These results demonstrate the different allelopathic activity of weed species extracts on alfalfa and suggest that weed may affect alfalfa growth and development through the inhibitory effects of allelochemicals present in weed tissue.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.1
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• pp.101-110
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• 2009

The vascular plants in the Mt. Hoemun were listed 541 taxa composed of 113 families, 354 genera, 473 species, 1 subspecies, 64 varieties and 3 forms. Divided into 541 taxa; woody plants were 151 taxa (27.9%) and herbaceous plants were 390 taxa (72.1%). Based on the list of rare plants by the Korea Forest Service, 10 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order: No. 159), Lilium callosum (No. 191), Tricyrtis dilatata (No. 97), Iris ensata var. sponianea (No. 197), Gastrodia elata (No.9), Aristolochia contorta (No. 151), Paeonia obovata (No. 30: Legal protection species), Prunus yedoensis (No. 110 : Planted species), Viola albida (No. 202), Schpolia japonica (No. 208). Based on the list of Korean endemic plants, 15 taxa were recorded; Cephalotaxus koreana, Lilium amabile, Salix purpurea var. japonica, Pseudostellaria multiflora, Aconitum pseudolaeve var. erectum, Filipendula glaberrima, Weigela subsessilis, etc. Based on the list of approved for delivering overseas of plants, 10 taxa were recorded; Carex okamotoi, Lilium distichum, Clematis trichotoma, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha; etc. Specific plant species by floral region were total 4S taxa (8.3% of all 541 taxa of vascular plants); Gastrodia elata, Paeonia obovata in class V, Carex arenicola, Corydalis grandicalyx in class IV, 5 taxa (Spiraea salicifolia, Schpolia japonica, Asperula lasiantha, etc.) in class III, 7 taxa (Lilium distichum, Iris ensata var. spontanea, Caryopteris incana, etc.) in class II and 28 taxa (Camptosorus sibiricus, Orixa japonica, Lonicera praeflorens, etc.) in class I. The naturalized plants in the surveyed sites were 13 families, 38 genera, 43 species, 2 varieties, 45 taxa (Phleum pratense, Medicago sativa, Sonchus asper, etc.) and naturalization rate was 8.3% of all 541 taxa of vascular plants. Ambrosia artemisiifolia var. elatior an ecosystem disturbing wild plants have been increasing. Therefore, the counter plants for continuous control and conservation are needed on the ecosystem of Mt. Hoemun.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.3
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• pp.141-146
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• 1990

Changes in dry matter yield, crude components, nutrients uptake($P_2O_5$, $K_2O$, CaO, MgO) and sulphur containing amino acid(cysteine, methionine) of orchardgrass(Dacty1is glomerata. L) and alfalfa(A4edicago sativa. L) by gypsum application(as sulphur source, 0, 2. 5, 10, 20kg SIlOa) were investigated to understand the effect of sulphur on herbage production in pasture, which was established in 1987 as means of hand broadcasting. The effect of gypsum on dry matter yield at different cutting times during growing seasons has not been found both in orchardgrass and in alfalfa, but in respect to annual total dry matter yield there were increment in herbage yield (P<0.05) of alfalfa at 5, 10, 20kg SIlOa in 1989 and the amounts of sulphur taken up in herbage slightly increased according to the rates of gypsum application. Maximum apparent recovery of sulphur was 7.55% at 2kg SIlOa in orchardgrass and was 17.8% at 5kg S/lOa in alfalfa. There were no any great differences in the content of crude components of both species and this trend was similar with the mineral contents of orchardgrass. But in alfalfa, the amounts of $K_2O$, CaO, and $P_2O_5$ taken up were increased by gypsum application and the increment in the amounts of minerals taken up in herbage at 20kg SIlOa were 14.9 of $K_2O$, 9.1 of CaO, and 2.5kgIlOa of $P_2O_5$ as compared to those of at untreated plot. Cysteine and methionine were not influenced by gypsum applicaton not only in orchardgrass but also cysteine in alfalfa, however, the content of methionine in alfalfa was slightly increased at 2, 5, lOkg SIlOa and at 20kg SIlOa was reverse.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.61-70
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• 2008

The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.313-322
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• 2007

The object of this experiment was to investigate the food habits of dairy goats fed with various roughage sources. The experimental trials were conducted at Chungnam National University in 2007. The experimental roughages include five sources and 25 species in all; grass sources: 5 species (Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass, legume sources: 5 species (Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources : 5 species (Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources: 5 species (Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea (Roxb.) Wight and Rumex crispus), and native grass sources: 5 species (Zoysia japonica Steudel, Agropyron tsukushiense var. transiens (Hack) Ohwi., Arundinella hirta (Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Ten dairy goats(Saanen) were selected which had nearly the same body weight(average 31kg). Experimental roughages were prepared by 0.5kg per 1 species fed to dairy goats, and the experiment was carried out from 09:00 to 18:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The dairy goats ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species order by dairy goat was observed like this: perennial ryegrass, tall fescue and red clover, respectively, and the lower intake species order was Rumex crispus. On the other hand, compared to each roughage source, total intake amount by dairy goats was showed as forage grasses (59.0%) which contained grasses(33.3%) and legumes (25.7%), browse (19.6%), weeds (15.9%) and native grasses (5.5%). Based on the result, the food intake type of dairy goats seems to be closer to grazer type.