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http://dx.doi.org/10.5333/KGFS.2019.39.4.298

Aluminum Stress Inhibits Root Growth and Alters Physiological and Antioxidant Enzyme Responses in Alfalfa (Medicago sativa L.) Roots  

Min, Chang-Woo (Division of Applied Life Sciences (BK21 Plus), IALS, Gyeongsang National University)
Khan, Inam (Division of Applied Life Sciences (BK21 Plus), IALS, Gyeongsang National University)
Lee, Byung-Hyun (Division of Applied Life Sciences (BK21 Plus), IALS, Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.39, no.4, 2019 , pp. 298-302 More about this Journal
Abstract
Acidic soil significantly reduces crop productivity mainly due to aluminum (Al) toxicity. Alfalfa (Medicago sativa L.) roots were exposed to aluminum stress (Al3+) in calcium chloride (CaCl2) solution (pH4.5) and root growth, physiological and antioxidant enzyme responses were investigated. The root growth (length) was significantly inhibited after 48 h of aluminum stress imposition. Histochemical staining with hematoxylin indicated significant accumulation of aluminum in Al stress-treated root tissues. Histochemical assay were also performed to detect superoxide anion, hydrogen peroxide and lipid peroxidation, which were found to be more in root tissues treated with higher aluminum concentrations. The enzymatic activity of CAT, POD and GR in root tissues was slightly increased after Al stress treatment. The result suggests that Al stress alters root growth in alfalfa and induces reactive oxygen species (ROS) production, and demonstrates that antioxidant enzymes involved in detoxification of Al-mediated oxidative stress.
Keywords
Alfalfa root; Aluminum; Antioxidant; ROS;
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